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Liu X.L.,Fujian Agriculture and forestry University | Liu X.L.,Yunnan Key Laboratory of Sugarcane Genetic Improvement | Liu X.L.,Yunnan Academy of Agricultural Sciences | Li X.J.,Yunnan Key Laboratory of Sugarcane Genetic Improvement | And 6 more authors.
Sugar Tech

Saccharum spontaneum L. plays a key role in the improvement of stress resistance and yield of sugarcane cultivars by serving as wild parent sources, especially lines of different ploidy. To better understand the genetic diversity and potential breeding value of S. spontaneum with different ploidy levels, 62 clones from four ploidy types (2n = 64, 72, 80, and 96) were screened using 30 pairs of SSR primers and with population genetics methods. The results showed that the decaploid and octaploid populations had a higher diversity than nonaploid and dodecaploid populations at 30 SSR loci with >98 % percentage of polymorphic bands and >0.92 polymorphic information content. Both the octaploid and decaploid populations shared more common bands with commonly used parents than the nonaploid or dodecaploid populations. The analysis of Nei’s genetic distance and UPGMA tree indicates that the decaploid population had the closest genetic relationship with the octaploid population and that the two populations had a close genetic relationship with commonly used parents (controls). According to the population divergence analysis, the four different ploidy populations did not show an obvious genetic divergence (Gst) (0.0880), but clearly had large amounts of gene flow (Nm) (5.1840). Octaploid and decaploid populations appeared to have undergone more genetic exchange with commonly used parents, as indicated by the low Gst value (0.1254 and 0.1276) and high Nm value (3.4872 and 3.4186). These results may provide important insight into utilizing the diversity of clones with different ploidy levels in S. spontaneum for future sugarcane breeding. © 2015 Society for Sugar Research & Promotion Source

Zhao P.,Yunnan Academy of Agricultural Sciences | Zhao P.,Yunnan Key Laboratory of Sugarcane Genetic Improvement | Todd J.,U.S. Department of Agriculture | Zhao J.,Yunnan Academy of Agricultural Sciences | And 17 more authors.

Enlarging the quantity of seedlings of elite families and discarding inferior sugarcane (Saccharum spp.) families could improve sugarcane breeding and selection efficiency. The feasibility of using the method Dynamic Technique for Order Preference by Similarity to Ideal Solution (DTOPSIS) method was explored to identify superior sugarcane families. Data on 5 traits: Brix, millable stalks per stool (MS), stalk diameter (SD), plant height (PH), and percent pith were collected from two family trials having 17 families and two check cultivars at two sites including plant-cane and frstratoon crops. The rest of the seedlings were planted into field for routine selection in the regular program. The DTOPSIS method calculates a comprehensive index (Ci) which expresses the closeness of a solution to the ideal solution and was used in this study to test the distance of each family to the ideal family. The Ci of the families was compared to the family selection rate in the regular program by determining the selection rate at Stage 1 to Stage 4 for each family in the regular program. The result indicated that the Ci values calculated from family trials were significantly (p<0.01) correlated to the selection rate at Stage 2 (r=0.8059), Stage3 (r=0.7967), and Stage 4 (r=0.8202), and indicating that promising clones were selected from families with higher Ci values in the family trial. Thus, it could be feasible to use DTOPSIS to determine elite sugarcane families and to eliminate inferior families and thereby, increasing the variety selection efficiency. © 2014, Instituto Agronomico. All rights reserved. Source

Liu X.-L.,Yunnan Key Laboratory of Sugarcane Genetic Improvement | Liu X.-L.,Yunnan Academy of Agricultural Sciences | Ma L.,Yunnan Key Laboratory of Sugarcane Genetic Improvement | Ma L.,Yunnan Academy of Agricultural Sciences | And 10 more authors.
Acta Agronomica Sinica

The purpose of this study was to provide simple sequence repeat (SSR)-based DNA fingerprinting information for sugarcane (Saccharum spp. Hybrids) cultivars that were developed in Yunan Province, China. Genomic DNAs from 27 cultivars were amplified with 8 pairs of polymorphic SSR primers. A total of 129 bands were obtained, including 123 polymorphic bands (95.35%). The mean value of polymorphic information content (PIC) was 0.9445, and the genetic similarity coefficient ranged from 0.269 to 0.767. Markers SMC1047HA and MSSCIR21 had the highest PIC values of 0.963 and could distinguish all the cultivars tested. Besides, primer combinations MSSCIR36/MSSCIR21, MSSCIR16/MSSCIR36, and MSSCIR36/SMC336BS were efficient in identifying the 27 cultivars, and their similar coefficients were lower than those of other primer combinations. These 3 primer combinations were validated with 27 cultivars and 10 staple commercial sugarcane cultivars in China, and MSSCIR16/MSSCIR36 was found to be optimal to construct DNA fingerprint identity (ID). Nomenclature of DNA fingerprint ID was suggested as serial number of National Nursery of Sugarcane Germplasm Resources (NNSGR) plus country and region code plus English initial abbreviation of breeding institution plus core primer name plus SSR marker data. This system is informative and useful in protecting new varieties and identifying cultivars because it consists of not only important information of a cultivar but also its specific molecular identify. © 2010 Crop Science Society of China and Institute of Crop Sciences, Chinese Academy of Agricultural Sciences. Source

Zhao P.,Yunnan Academy of Agricultural Sciences | Zhao P.,Yunnan Key Laboratory of Sugarcane Genetic Improvement | Liu J.,Yunnan Academy of Agricultural Sciences | Liu J.,Yunnan Key Laboratory of Sugarcane Genetic Improvement | And 12 more authors.
International Sugar Journal

Yunnan is the second largest sugarcane growing area in China. Due to the rugged nature of the province, there is usually a lag between harvest and replanting and milling. Staling of cane invariably leads to losses for both cane farmers and sugar millers. A reasonable way to avoid the losses is to select cultivars with good staling-resistance. In this study five sugarcane genotypes were staled for 0, 7, 14 or 21 days. Parameters describing characters of quality deterioration and sprouting were elicited with dynamic technique for order preference by similarity to ideal solution (DTOPSIS) to evaluate the staling-resistance of the five genotypes. Findings indicated that parameters varied not only with length of staling but also with genotype, which suggested the possibility of selecting for stalingresistant ability in sugarcane. The score calculated by DTOPSIS indicated the order of staling-resistance among the genotypes was C266-70 > CYZ04-320 > CYZ04-724 > CYZ05-301 > CYZ05-427. As far as the staling-resistance is concerned, C266-70 and CYZ04-320 were identified as most suitable for the Yunnan Province and similar landforms. Source

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