Yunnan Institute of Microbiology

Kunming, China

Yunnan Institute of Microbiology

Kunming, China
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Nie G.-X.,Henan Normal University | Ming H.,Xinxiang Medical University | Ming H.,Yunnan Institute of Microbiology | Zhang J.,Yunnan Institute of Microbiology | And 7 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2012

An orange-coloured, non-spore-forming, motile and coccus-shaped actinobacterium, designated YIM 75677 T, was isolated from a soil sample collected from a dry-hot river valley in Dongchuan county, Yunnan Province, south-west China and its taxonomic position was investigated. Growth of strain YIM 75677 T occurred at 12-55 °C, pH 6.0-9.0 and NaCl tolerance up to 2 % (w/v). Cells adhered to agar media and were agglutinated tightly together. The peptidoglycan contained meso-diaminopimelic acid, alanine and glutamic acid. The whole-cell hydrolysates mainly contained glucose, galactose, mannose and ribose. The predominant menaquinone was MK-9 (H 2) and the major fatty acids were anteiso-C 15:0 and iso-C 15:0. Mycolic acids were not present. The DNA G?C content of strain YIM 75677 T was 74.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons clearly revealed that strain YIM 75677 T represents a novel member of the genus Kineococcus and is closely related to Kineococcus xinjiangensis S2-20 T (level of similarity, 98.6 %). Meanwhile, the result of DNA-DNA hybridization between strain YIM 75677 T and K. xinjiangensis S2-20 T demonstrated that this isolate represented a different genomic species in the genus Kineococcus. On the basis of phylogenetic, phenotypic andchemotaxonomic characteristics, strain YIM 75677 T represents a novel species of the genus Kineococcus, for which the name Kineococcus glutineturens sp. nov. is proposed. The type strain is YIM 75677 T (=CCTCC AA 209075T = JCM 18126 T). © Springer Science+Business Media B.V. 2012.


Ming H.,Xinxiang Medical University | Ming H.,Yunnan Institute of Microbiology | Nie G.-X.,Henan Normal University | Jiang H.-C.,Wuhan University | And 6 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2012

A novel cold-resistant bacterium, designated YIM 016 T, was isolated from a peat bog sample collected from Mohe County, Heilongjiang Province, Northern China and its taxonomic position was investigated using a polyphasic approach. The strain was Gram-positive, aerobic, endospore-forming, motile and rod-shaped. Phylogenetic analyses based on the 16S rRNA gene sequence clearly revealed that strain YIM 016 T is a member of the genus Paenibacillus. The strain is closely related to Paenibacillus alginolyticus DSM 5050 T, Paenibacillus chondroitinus DSM 5051 T and Paenibacillus pocheonensis Gsoil 1138 T with similarities of 99.0 %, 97.0 % and 96.3 %, respectively. Meanwhile, the low DNA-DNA relatedness levels between strain YIM 016 T and its closely related phylogenetic neighbours demonstrated that this isolate represents a new genomic species in the genus Paenibacillus. Phenotypic and chemotaxonomic tests showed that growth of strain YIM 016 T occurred at 4-37 °C, pH 6.0-8.0 and with a NaCl tolerance up to 0.5 % (w/v). The peptidoglycan contained meso-diaminopimelic acid, alanine and glutamic acid. The whole-cell hydrolysates mainly contained glucose, galactose and ribose. The predominant menaquinone was MK-7 and the major fatty acids were anteiso-C 15:0 and iso-C 16:0. The DNA G+Ccontent of strain YIM 016 T was 51.7 mol %. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain YIM 016 T could be clearly distinguished from other species of the genus Paenibacillus. It is therefore concluded that strain YIM 016 T represents a novel species in the genus Paenibacillus, for which the name Paenibacillus frigoriresistens sp. nov. is proposed. The type strain is YIM 016 T (= CCTCC AB 2011150 T = JCM 18141 T). © Springer Science+Business Media B.V. 2012.


Yuan L.-J.,Peking Union Medical College | Yuan L.-J.,Tangshan College | Zhang Y.-Q.,Peking Union Medical College | Yu L.-Y.,Peking Union Medical College | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

An actinomycete, strain I06-2230T, was isolated from rhizosphere soil of the plant Cephalotaxus fortunei, collected from Yunnan province, south China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Actinopolymorpha. Cells grew on agar surfaces, with no penetration even after prolonged cultivation. Aerial hyphae were absent. Cells were irregularly shaped and remained attached as chains or aggregates. Chemotaxonomic data, which showed LL-diaminopimelic acid in the cell wall, glucose as the whole-cell sugar, type PI phospholipids and MK-9(H4) as the predominant menaquinone, supported the affiliation of strain I06-2230T to the genus Actinopolymorpha. The major fatty acids were iso-C15:0, iso-C16:0 and iso-C16:1 H. The genomic DNA G+C content was 69.3 mol%. DNA-DNA hybridization data, in combination with chemotaxonomic, physiological and biochemical data, demonstrated that strain I06-2230T should be classified as representing a novel species of the genus Actinopolymorpha. The name Actinopolymorpha cephalotaxi sp. nov. is proposed, with strain I06-2230T (=DSM 45117T =CCM 7466T =KCTC 19293T) as the type strain. © 2010 IUMS.


Prabhu D.M.,Sun Yat Sen University | Prabhu D.M.,Gulbarga University | Quadri S.R.,University of Medical Sciences and Technology | Cheng J.,Yunnan Institute of Microbiology | And 7 more authors.
Journal of Antibiotics | Year: 2015

A moderate growing strain designated as MPKL 26T was isolated from a soil sample of Bidar Fort, Karnataka, India. The strain MPKL 26T was Gram positive, bent rod in shape. The optimum pH and temperature for growth was 7.0 and 30 °C, respectively. The 16S ribosomal RNA gene sequence analysis revealed that strain MPKL 26T was closely related to S. atrocyanea DSM 20127T (98.09%), S. flava CW 108T (98.04%), S. soli CW 59T (97.99%) and S. notoginsengisoli SYP-B575T (97.0%) and showed DNA-DNA hybridization relatedness (46.05±1.2, 33.56±2.55, 32.56±1.7 and 26.79±2.5, respectively, between these strains) less than the threshold value for the delineation of genomic species. The peptidoglycon type was A3α type with glycine, alanine, lysine and glutamic acid as the amino acids. The whole-cell sugars ere fructose, ribose, mannose, glucose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol along with three unknown polar lipids. The fatty acid profile contained C14:0, C16:0, iso-C14:0, iso-C15:0, iso-C16:0, iso-C17:0, anteiso-C15:0, anteiso-C17:0 and summed feature 4 (17:1 iso I/anteiso B). The predominant respiratory quinine was MK-9(H2) with MK-10(H2), MK-8(H2) and MK-8(H4) as minor respiratory quinines. The G+C content of the genomic DNA was 68.8 mol%. On the basis of phenotypic, chemotaxonomic and molecular characteristics, the strain MPKL 26T represents a novel species of the genus Sinomonas, for which the name Sinomonas mesophila sp. nov. is proposed with MPKL 26T as the type strain (=NCIM 5552T= JCM 30094T). © 2015 Japan Antibiotics Research Association All rights reserved.


Pan H.-Q.,CAS Shenyang Institute of Applied Ecology | Cheng J.,Yunnan Institute of Microbiology | Zhang D.-F.,Yunnan Institute of Microbiology | Yu S.-Y.,CAS Shenyang Institute of Applied Ecology | And 7 more authors.
Journal of Antibiotics | Year: 2015

A novel actinomycete strain, designated 11A07 T, was isolated from young Scomberomorus niphonius in the Bohai Sea. Basic local alignment search tool analyses showed that this isolate had the highest 16S rRNA gene sequence similarity of 97.41% with Streptomyces rimosus subsp. paromomycinus DSM 41429 T. Phylogenetic tree revealed that strain 11A07 T formed a distinct lineage clustered with Streptomyces panacagri Gsoil 519 T, Streptomyces sodiiphilus YIM 80305 T and Streptomyces albus subsp. albus NRRL B-2365 T having similarities of 97.30%, 97.10% and 96.83%, respectively. Multilocus sequence analysis further demonstrated that the new isolate was different from the selected representatives of Streptomyces as a separate phylogenetic line. Strain 11A07 T produced straight or rectiflexibile spore chains with smooth surface, white aerial mycelia and brown diffusible pigments on international streptomyces project 2 medium. Maximum tolerated NaCl concentration for growth was 11.0%. Whole-cell sugars were mannose, ribose, glucose, galactose and xylose. The predominant menaquinones were MK-9(H2), MK-9(H4) and MK-9 (H6). The fatty-acid profile contained iso-C16:0, C 18:0 10-methyl (tuberculostearic acid) and anteiso-C17:0 as the major compositions. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unknown phospholipid. The G+C content of the genomic DNA was 71.4 mol%. These morphological, phenotypic and chemotaxonomic properties showed that strain 11A07 T could be readily distinguished from the most closely related members of the genus Streptomyces. Thus, based on the polyphasic taxonomic data, strain 11A07 T (=JCM 19630 T =CCTCC AA 2013020 T =KCTC 29263 T) represents a novel species within the genus Streptomyces, for which the name Streptomyces bohaiensis sp. nov. is proposed.


Wang Z.,Nanjing Agricultural University | Xu J.,Nanjing Agricultural University | Li Y.,Yunnan Institute of Microbiology | Wang K.,Nanjing Agricultural University | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

A novel carbendazim-degrading actinobacterium, designated djl-6-2T, was isolated from the sludge of a carbendazim wastewater treatment facility in Jiangsu province, China. The morphological and chemotaxonomic properties of the isolate were typical of members of the genus Rhodococcus. Strain djl-6-2T formed a coherent cluster with Rhodococcus qingshengii djl-6T, Rhodococcus baikonurensis DSM 44587T, Rhodococcus erythropolis DSM 43066T and Rhodococcus globerulus DSM 43954T in 16S rRNA gene sequence analysis. The results of DNA-DNA hybridization with the above strains (27.7, 19.3, 18.6 and 10.6% relatedness, respectively), in combination with differences in biochemical and physiological properties, suggest that strain djl-6-2T should be classified within a novel species of the genus Rhodococcus, for which the name Rhodococcus jialingiae sp. nov. is proposed, with djl-6-2T (=DSM 45257T =CCTCC AB 208292T) as the type strain. © 2010 IUMS.


Qin S.,Jiangsu University | Yuan B.,Jiangsu University | Zhang Y.-J.,Jiangsu University | Bian G.-K.,Jiangsu University | And 4 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2012

A novel Gram-positive, aerobic, rodshaped and mycelia-producing bacterial strain, designated KLBMP 1050 T, was isolated from the stem of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate KLBMP 1050 T belonged to the genus Nocardioides, with the highest sequence similarity to Nocardioides albus KCTC 9186 T (99.38 %) and Nocardioides luteus KCTC 9575 T (99.03 %). However, the DNA-DNA relatedness of isolate KLBMP 1050 T to these two type strains were 37.5 ± 3.5 and 33 ± 2.3 %, respectively. Strain KLBMP 1050 T grew at the pH range 6-11, temperature range 10-32 °C and with 0-12 % NaCl. The physiological properties of strain KLBMP 1050 T differ from those of N. albus KCTC 9186 T and N. luteus KCTC 9575 T. The cell-wall peptidoglycan contained LL-diaminopimelic acid and MK-8(H 4) was the major respiratory quinone. The predominant cellular fatty acid of strain KLBMP 1050 T was iso-C16:0 (23.3 %). The total DNA G+C content was 70.1 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain KLBMP 1050 T represents a novel species of the genus Nocardioides, for which the name Nocardioides panzhihuaensis sp. nov. is proposed. The type strain is KLBMP 1050 T (= KCTC 19888 T = NBRC 108680 T. © Springer Science+Business Media B.V. 2012.


Tian X.-P.,CAS South China Sea Institute of Oceanology | Xu Y.,Yunnan Institute of Microbiology | Zhang J.,Yunnan Institute of Microbiology | Li J.,CAS South China Sea Institute of Oceanology | And 5 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2012

A novel aerobic actinomycete strain, designated as SCSIO 02100 T, was isolated from a deep sea sediment sample collected from Northern South China Sea at a depth of 578 m. This isolate requires sea water or a sodium-supplemented medium for growth. BLAST searches based on the almost full length of the 16S rRNA gene sequence, showed that strain SCSIO 02100 T had the highest similarities with Streptomyces armeniacus (JCM 3070 T) (97.1 %). Phylogenetic trees reconstructed on the basis of 16S rRNA gene sequences revealed that strain SCSIO 02100 T formed a distinct lineage with S. nanshensis SCSIO 01066 T with 96.9 % similarity. Further analysis of the polyphasic taxonomic data, including morphological, phenotypic and chemotaxonomic properties, showed that strain SCSIO 02100 T could be readily distinguished from the most closely related members of the genus Streptomyces. Thus, based on the polyphasic taxonomic data, a novel species, Streptomyces oceani sp. nov., is proposed, with the type strain SCSIO 02100 T (=DSM 42043 T = CGMCC 4.7007 T). © Springer Science+Business Media B.V. 2012.


Bian G.-K.,Jiangsu University | Qin S.,Jiangsu University | Yuan B.,Jiangsu University | Zhang Y.-J.,Jiangsu University | And 4 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2012

A novel actinomycete, designated strain KLBMP 4601 T, was isolated from the root of the medicinal plant Curcuma phaeocaulis collected from Sichuan Province, south-west China. The strain produced extensively branched substrate and aerial hyphae that carried straight to flexuous spore chains. Chemotaxonomic properties of this strain were consistent with those of members of the genus Streptomyces. The cell wall of strain KLBMP 4601 T contained LL-diaminopimelic acid as the characteristic diamino acid. The major menaquinone was MK-9(H 4), with minor amounts of MK-9(H 6), MK-9(H 8) and MK-10(H 2). The major fatty acids were C 16:0, iso-C 16:0, C18:1x9c and C 16:1, iso G. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KLBMP 4601 T belongs to the genus Streptomyces and is most closely related to Streptomyces armeniacus JCM 3070 T (97.9 %), Streptomyces pharmamarensis PM267 T (97.6 %) and Streptomyces artemisiae YIM 63135T (97.5 %). The 16S rRNA gene sequence similarity between strain KLBMP 4601 T and other members of this genus were lower than 97.5 %. DNA-DNA hybridization studies of strain KLBMP 4601 T with the three closest species showed relatedness values of 36.3 ± 4.2 %, 27.3 ± 0.6 %, and 30.9 ± 2.5 %, respectively. On the basis of chemotaxonomic, phenotypic and genotypic characteristics, it is evident that strain KLBMP 4601 T represents a novel species of the genus Streptomyces, for which the name Streptomyces phytohabitans sp. nov. is proposed. The type strain is KLBMP 4601 T (=KCTC 19892 T = NBRC 108772 T). © Springer Science+Business Media B.V. 2012.


Dong J.,Yunnan University | Zhao L.,Yunnan Institute of Microbiology | Cai L.,Yunnan University | Fang H.,Yunnan University | And 2 more authors.
Journal of Bioscience and Bioengineering | Year: 2014

The tubers of Bletilla formosana were fermented with eight plant pathogen fungi, respectively, and antioxidant activities and total phenolic content (TPC) of the crude extracts of fermented products and non-fermented products were investigated. The antioxidant activities were evaluated in three different test systems [DPPH, ABTS radical-scavenging activity, and ferric reducing-antioxidant power (FRAP)]. It was found that the extract of Helminthosporium maydis fermented B.formosana (FBF) possessed the highest TPC and exhibited a significant antioxidant activity compared with non-fermented product and other fermented products. Correlation analysis between antioxidant activities and TPC was also investigated. The good correlation between antioxidant activities and TPC revealed that the phenolic compounds might be the major contributors for the high antioxidant activities of the fermented B.formosana. Two phenolic compounds, curvularin and dehydrocurvularin, were isolated from H.maydis FBF, which had never been reported from plant of orchidaceae or H.maydis. Curvularin exhibited significant antioxidant activities, and was also present at a high concentration (0.373mg/mg extract sample), implying an important role for the antioxidant activity of H.maydis FBF. This study suggested that proper fermentation processing could improve TPC and antioxidant activities of B.formosana. © 2014 The Society for Biotechnology, Japan.

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