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Bian G.-K.,Jiangsu University | Qin S.,Jiangsu University | Yuan B.,Jiangsu University | Zhang Y.-J.,Jiangsu University | And 4 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

A novel actinomycete, designated strain KLBMP 4601 T, was isolated from the root of the medicinal plant Curcuma phaeocaulis collected from Sichuan Province, south-west China. The strain produced extensively branched substrate and aerial hyphae that carried straight to flexuous spore chains. Chemotaxonomic properties of this strain were consistent with those of members of the genus Streptomyces. The cell wall of strain KLBMP 4601 T contained LL-diaminopimelic acid as the characteristic diamino acid. The major menaquinone was MK-9(H 4), with minor amounts of MK-9(H 6), MK-9(H 8) and MK-10(H 2). The major fatty acids were C 16:0, iso-C 16:0, C18:1x9c and C 16:1, iso G. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KLBMP 4601 T belongs to the genus Streptomyces and is most closely related to Streptomyces armeniacus JCM 3070 T (97.9 %), Streptomyces pharmamarensis PM267 T (97.6 %) and Streptomyces artemisiae YIM 63135T (97.5 %). The 16S rRNA gene sequence similarity between strain KLBMP 4601 T and other members of this genus were lower than 97.5 %. DNA-DNA hybridization studies of strain KLBMP 4601 T with the three closest species showed relatedness values of 36.3 ± 4.2 %, 27.3 ± 0.6 %, and 30.9 ± 2.5 %, respectively. On the basis of chemotaxonomic, phenotypic and genotypic characteristics, it is evident that strain KLBMP 4601 T represents a novel species of the genus Streptomyces, for which the name Streptomyces phytohabitans sp. nov. is proposed. The type strain is KLBMP 4601 T (=KCTC 19892 T = NBRC 108772 T). © Springer Science+Business Media B.V. 2012. Source

Dong J.,Yunnan University | Zhao L.,Yunnan Institute of Microbiology | Cai L.,Yunnan University | Fang H.,Yunnan University | And 2 more authors.
Journal of Bioscience and Bioengineering

The tubers of Bletilla formosana were fermented with eight plant pathogen fungi, respectively, and antioxidant activities and total phenolic content (TPC) of the crude extracts of fermented products and non-fermented products were investigated. The antioxidant activities were evaluated in three different test systems [DPPH, ABTS radical-scavenging activity, and ferric reducing-antioxidant power (FRAP)]. It was found that the extract of Helminthosporium maydis fermented B.formosana (FBF) possessed the highest TPC and exhibited a significant antioxidant activity compared with non-fermented product and other fermented products. Correlation analysis between antioxidant activities and TPC was also investigated. The good correlation between antioxidant activities and TPC revealed that the phenolic compounds might be the major contributors for the high antioxidant activities of the fermented B.formosana. Two phenolic compounds, curvularin and dehydrocurvularin, were isolated from H.maydis FBF, which had never been reported from plant of orchidaceae or H.maydis. Curvularin exhibited significant antioxidant activities, and was also present at a high concentration (0.373mg/mg extract sample), implying an important role for the antioxidant activity of H.maydis FBF. This study suggested that proper fermentation processing could improve TPC and antioxidant activities of B.formosana. © 2014 The Society for Biotechnology, Japan. Source

Yuan L.-J.,Peking Union Medical College | Yuan L.-J.,Tangshan College | Zhang Y.-Q.,Peking Union Medical College | Yu L.-Y.,Peking Union Medical College | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology

An actinomycete, strain I06-2230T, was isolated from rhizosphere soil of the plant Cephalotaxus fortunei, collected from Yunnan province, south China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Actinopolymorpha. Cells grew on agar surfaces, with no penetration even after prolonged cultivation. Aerial hyphae were absent. Cells were irregularly shaped and remained attached as chains or aggregates. Chemotaxonomic data, which showed LL-diaminopimelic acid in the cell wall, glucose as the whole-cell sugar, type PI phospholipids and MK-9(H4) as the predominant menaquinone, supported the affiliation of strain I06-2230T to the genus Actinopolymorpha. The major fatty acids were iso-C15:0, iso-C16:0 and iso-C16:1 H. The genomic DNA G+C content was 69.3 mol%. DNA-DNA hybridization data, in combination with chemotaxonomic, physiological and biochemical data, demonstrated that strain I06-2230T should be classified as representing a novel species of the genus Actinopolymorpha. The name Actinopolymorpha cephalotaxi sp. nov. is proposed, with strain I06-2230T (=DSM 45117T =CCM 7466T =KCTC 19293T) as the type strain. © 2010 IUMS. Source

Wang Z.,Nanjing Agricultural University | Xu J.,Nanjing Agricultural University | Li Y.,Yunnan Institute of Microbiology | Wang K.,Nanjing Agricultural University | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology

A novel carbendazim-degrading actinobacterium, designated djl-6-2T, was isolated from the sludge of a carbendazim wastewater treatment facility in Jiangsu province, China. The morphological and chemotaxonomic properties of the isolate were typical of members of the genus Rhodococcus. Strain djl-6-2T formed a coherent cluster with Rhodococcus qingshengii djl-6T, Rhodococcus baikonurensis DSM 44587T, Rhodococcus erythropolis DSM 43066T and Rhodococcus globerulus DSM 43954T in 16S rRNA gene sequence analysis. The results of DNA-DNA hybridization with the above strains (27.7, 19.3, 18.6 and 10.6% relatedness, respectively), in combination with differences in biochemical and physiological properties, suggest that strain djl-6-2T should be classified within a novel species of the genus Rhodococcus, for which the name Rhodococcus jialingiae sp. nov. is proposed, with djl-6-2T (=DSM 45257T =CCTCC AB 208292T) as the type strain. © 2010 IUMS. Source

Prabhu D.M.,Sun Yat Sen University | Prabhu D.M.,Gulbarga University | Quadri S.R.,University of Medical Sciences and Technology | Cheng J.,Yunnan Institute of Microbiology | And 7 more authors.
Journal of Antibiotics

A moderate growing strain designated as MPKL 26T was isolated from a soil sample of Bidar Fort, Karnataka, India. The strain MPKL 26T was Gram positive, bent rod in shape. The optimum pH and temperature for growth was 7.0 and 30 °C, respectively. The 16S ribosomal RNA gene sequence analysis revealed that strain MPKL 26T was closely related to S. atrocyanea DSM 20127T (98.09%), S. flava CW 108T (98.04%), S. soli CW 59T (97.99%) and S. notoginsengisoli SYP-B575T (97.0%) and showed DNA-DNA hybridization relatedness (46.05±1.2, 33.56±2.55, 32.56±1.7 and 26.79±2.5, respectively, between these strains) less than the threshold value for the delineation of genomic species. The peptidoglycon type was A3α type with glycine, alanine, lysine and glutamic acid as the amino acids. The whole-cell sugars ere fructose, ribose, mannose, glucose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol along with three unknown polar lipids. The fatty acid profile contained C14:0, C16:0, iso-C14:0, iso-C15:0, iso-C16:0, iso-C17:0, anteiso-C15:0, anteiso-C17:0 and summed feature 4 (17:1 iso I/anteiso B). The predominant respiratory quinine was MK-9(H2) with MK-10(H2), MK-8(H2) and MK-8(H4) as minor respiratory quinines. The G+C content of the genomic DNA was 68.8 mol%. On the basis of phenotypic, chemotaxonomic and molecular characteristics, the strain MPKL 26T represents a novel species of the genus Sinomonas, for which the name Sinomonas mesophila sp. nov. is proposed with MPKL 26T as the type strain (=NCIM 5552T= JCM 30094T). © 2015 Japan Antibiotics Research Association All rights reserved. Source

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