Yunnan Forestry Technological College

Kunming, China

Yunnan Forestry Technological College

Kunming, China
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Zhu J.-Y.,Southwest forestry University | Wu G.-X.,Yunnan Agricultural University | Ze S.-Z.,Yunnan Forestry Technological College | Stanley D.W.,U.S. Department of Agriculture | Yang B.,Southwest forestry University
Journal of Insect Physiology | Year: 2014

Ectoparasitoid wasps deposit their eggs onto the surface and inject venom into their hosts. Venoms are chemically complex and they exert substantial impact on hosts, including permanent or temporary paralysis and developmental arrest. These visible venom effects are due to changes in expression of genes encoding physiologically relevant proteins. While the influence of parasitization on gene expression in several lepidopterans has been reported, the molecular details of parasitoid/beetle relationships remain mostly unknown. This shortcoming led us to pose the hypothesis that envenomation by the ectoparasitic ant-like bethylid wasp Scleroderma guani leads to changes in protein expression in the yellow mealworm beetle Tenebrio molitor. We tested our hypothesis by comparing the proteomes of non-parasitized and parasitized host pupae using iTRAQ-based proteomics. We identified 41 proteins that were differentially expressed (32↑- and 9↓-regulated) in parasitized pupae. We assigned these proteins to functional categories, including immunity, stress and detoxification, energy metabolism, development, cytoskeleton, signaling and others. We recorded parallel changes in mRNA levels and protein abundance in 14 selected proteins following parasitization. Our findings support our hypothesis by documenting changes in protein expression in parasitized hosts. © 2014 Elsevier Ltd.


PubMed | Nanjing Agricultural University, U.S. Department of Agriculture and Yunnan Forestry Technological College
Type: | Journal: Horticulture research | Year: 2015

Pineapple (Ananas comosus [L.] Merr.) is the third most important tropical fruit in the world after banana and mango. As a crop with vegetative propagation, genetic redundancy is a major challenge for efficient genebank management and in breeding. Using expressed sequence tag and nucleotide sequences from public databases, we developed 213 single nucleotide polymorphism (SNP) markers and validated 96 SNPs by genotyping the United States Department of Agriculture - Agricultural Research Service pineapple germplasm collection, maintained in Hilo, Hawaii. The validation resulted in designation of a set of 57 polymorphic SNP markers that revealed a high rate of duplicates in this pineapple collection. Twenty-four groups of duplicates were detected, encompassing 130 of the total 170 A cosmos accessions. The results show that somatic mutation has been the main source of intra-cultivar variations in pineapple. Multivariate clustering and a model-based population stratification suggest that the modern pineapple cultivars are comprised of progenies that are derived from different wild Ananas botanical varieties. Parentage analysis further revealed that both A. comosus var. bracteatus and A. comosus var. ananassoides are likely progenitors of pineapple cultivars. However, the traditional classification of cultivated pineapple into horticultural groups (e.g. Cayenne, Spanish, Queen) was not well supported by the present study. These SNP markers provide robust and universally comparable DNA fingerprints; thus, they can serve as an efficient genotyping tool to assist pineapple germplasm management, propagation of planting material, and pineapple cultivar protection. The high rate of genetic redundancy detected in this pineapple collection suggests the potential impact of applying this technology on other clonally propagated perennial crops.


Li G.-F.,Yunnan Forestry Technological College | Wang C.-M.,Mojiang County Nationality Middle School | Li H.-W.,Yunnan Forestry Technological College | Hou Y.-P.,Yunnan Forestry Technological Promotion Station
Zootaxa | Year: 2015

Oxyporus (Oxyporus) kunmingius sp. nov. is described from Yunnan, China. Color images of the habitus and aedeagus of the new species are included. A key to the genus Oxyporus of Yunnan species is provided. Copyright © 2015 Magnolia Press.


PubMed | Yunnan Forestry Technological Promotion Station, Yunnan Forestry Technological College and Mojiang County Nationality Middle School
Type: Journal Article | Journal: Zootaxa | Year: 2015

Oxyporus (Oxyporus) kunmingius sp. nov. is described from Yunnan, China. Color images of the habitus and aedeagus of the new species are included. A key to the genus Oxyporus of Yunnan species is provided.


PubMed | Kunming Nengxun Science and Technology Ltd Company, Yunnan Forestry Technological Promotion Station, Yunnan Forestry Technological College and Mojiang County Nationality Middle School
Type: Letter | Journal: Zootaxa | Year: 2015

Oxyporus (Oxyporus) bingshengae sp. nov. in Yunnan, China, is described. Color images of the habitus and aedeagus of the new species are included. A key to the species of Oxyporus of Yunnan is provided.


Zhu J.-Y.,Southwest forestry University | Ze S.-Z.,Yunnan Forestry Technological College | Yang B.,Southwest forestry University
Journal of Asia-Pacific Entomology | Year: 2015

Chemosensory proteins (CSPs) are believed to be important in the detection of semiochemicals, and have been reported to be involved in development, molting, leg regeneration and phase transition. In the current study, six CSP genes were identified in the beet armyworm Spodoptera exigua, namely SexiCSP1-6, from the full length cDNA library. Multiple alignment and phylogenetic analysis indicated that they were very diverse as seen in CSPs of other insect species. Expression patterns determined by quantitative real-time PCR (qPCR) revealed that they were highly expressed in antenna. SexiCSP1-3, SexiCSP5 and SexiCSP6 were male antenna-biased, while SexiCSP4 was female-biased in the antenna. In addition to antenna, they were expressed at high levels in legs and wings, suggesting potential roles in contact chemoreception and non-olfactory roles. Compared with the expression in antenna, legs and wings, only SexiCSP1 showed relatively high expression in heads, thoraxes and abdomen. These results extended olfactory gene resource in the beet armyworm and provided basis for further gaining a better understanding of CSPs roles in this pest. © 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.


Du H.-Y.,Changchun University of Science and Technology | Wei Z.-P.,Changchun University of Science and Technology | Sun L.-J.,Yunnan Forestry Technological College | Chu X.-Y.,Changchun University of Science and Technology | And 5 more authors.
Chinese Optics | Year: 2013

Mn-doped ZnS nanoparticles were prepared by the Sol-Gel process in this paper, and the influence of doping ion concentration on crystal structures and luminescent properties of ZnS:[KG-*3/5]Mn nanoparticles was discussed. The structures of the samples were characterized by an X-ray diffractometer(XRD). The results show that the as-prepared ZnS:[KG-*3/5]Mn nanoparticles belong to the cubic sphalerite structure. The parvafacies do not occur when Mn2+-doping concentration reaches 6% and the average particle sizes of nanoparticles decrease with the increase of doping concentration. Photoluminescence(PL) spectroscopy and fluorescence spectrum results show that the emission wavelengths of ZnS:[KG-*3/5]Mn nanoparticles around 590 nm can be adjusted by changing the concentration of the ions. In addition, the influence of temperature on morphology and luminescent properties of nanoparticles was studied. The result observed from a high resolution transmission electron microscopy(HRTEM) shows that the average particle sizes of ZnS:[KG-*3/5]Mn samples increase to about 20 nm after ageing for 1 h at the temperature of 50 °C. The heating ageing is beneficial to Mn2+ fluorescence produced at 590 nm for ZnS:[KG-*3/5]Mn nanoparticles.


PubMed | Chinese Academy of Forestry, Bureau of Forest Pest Control and Quarantine of Yunnan Province and Yunnan Forestry Technological College
Type: | Journal: Insect biochemistry and molecular biology | Year: 2016

Pheromone-binding proteins (PBPs) play an important role in the recognition of pheromones by insects. However, the abilities of these PBPs to discriminate pheromone components and recognize the isomers are unclear. Dendrolimus houi and Dendrolimus kikuchii are two sympatric coniferous pests whose pheromones have cis-trans isomers. We used these insect species to detect the precise recognition abilities of PBPs. The four PBPs examined showed male-biased antenna-intensive expression patterns, whereas PBP1 showed higher expression than PBP2 in the antenna. DhouPBP1 only bound to a minor interspecific pheromone component, whereas DhouPBP2 bound to all three intraspecific components and another minor interspecific component. DkikPBP1 and DkikPBP2 could recognize all three intraspecific components with affinities negatively correlated with their ratios, and they bound to interspecific pheromones with affinity that was positively correlated with the ratios. The four PBPs have different cis-trans isomer discrimination abilities, i.e., DhouPBP1 and DkikPBP1 could not discriminate the two cis-trans isomer pairs of pheromones from the two species, whereas DhouPBP2 could discriminate between both pairs, and DkikPBP2 could only discriminate one pair. Overall, PBPs from D.houi and D.kikuchii use different strategies to help the moths to discriminate the intra- and interspecific pheromone components. Our work will contribute to better understanding of the sex pheromone recognition mechanism in these two sister species of moths and provide insights into more effective management practices of these pest species.


PubMed | University of Basel, Yunnan Forestry Technological College and CAS Kunming Institute of Botany
Type: | Journal: Scientific reports | Year: 2016

A prerequisite for the evolutionary stability of pollinating seed-consuming mutualisms is that each partner benefits from the association. However, few studies of such mutualism have considered the benefit gained by the pollinators. Here, we determined how the pollinating seed-predators ensure the provisioning of their offspring in the recently discovered mutualism between Rheum nobile and Bradysia flies. The correlation between flower fate and fly oviposition was examined. Floral traits and patterns of variation in fruit abortion and fly oviposition were investigated to determine whether female flies exhibit preferences for particular flowers when laying eggs. Indole-3-acetic acid (IAA) was quantified to determine whether female flies manipulate host physiology. Flowers that flies oviposited on had a significantly lower probability of fruit abortion compared with intact flowers. Females did not exhibit oviposition preference for any of the floral traits examined. There was no significant correlation between fruit abortion and fly oviposition in terms of either flower position or timing of flowering. IAA concentrations in oviposited flowers were significantly higher than in intact flowers. Our results suggest that oviposition by the mutualistic seed-consuming pollinator Bradysia sp., greatly reduces the probability of fruit abortion of its host, R. nobile; this may be attributed to the manipulation of host physiology through regulating IAA levels.


PubMed | Yunnan Forestry Technological College
Type: | Journal: Zootaxa | Year: 2015

Plastus (Stigmatochirus) menglaius sp. nov. is described from Yunnan, China. Color images of the habitus and aedeagu of the new species are included. A key to the subgenus Stigmatochirus of World species is provided.

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