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Zhu J.-Y.,Southwest forestry University | Ze S.-Z.,Yunnan Forestry Technological College | Yang B.,Southwest forestry University
Journal of Asia-Pacific Entomology | Year: 2015

Chemosensory proteins (CSPs) are believed to be important in the detection of semiochemicals, and have been reported to be involved in development, molting, leg regeneration and phase transition. In the current study, six CSP genes were identified in the beet armyworm Spodoptera exigua, namely SexiCSP1-6, from the full length cDNA library. Multiple alignment and phylogenetic analysis indicated that they were very diverse as seen in CSPs of other insect species. Expression patterns determined by quantitative real-time PCR (qPCR) revealed that they were highly expressed in antenna. SexiCSP1-3, SexiCSP5 and SexiCSP6 were male antenna-biased, while SexiCSP4 was female-biased in the antenna. In addition to antenna, they were expressed at high levels in legs and wings, suggesting potential roles in contact chemoreception and non-olfactory roles. Compared with the expression in antenna, legs and wings, only SexiCSP1 showed relatively high expression in heads, thoraxes and abdomen. These results extended olfactory gene resource in the beet armyworm and provided basis for further gaining a better understanding of CSPs roles in this pest. © 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.

Song B.,CAS Kunming Institute of Botany | Stocklin J.,University of Basel | Peng D.,CAS Kunming Institute of Botany | Gao Y.,Yunnan Forestry Technological College | Sun H.,CAS Kunming Institute of Botany
Botanical Journal of the Linnean Society | Year: 2015

Environmental conditions are known to affect the reproductive fitness of insects, as do host plants. Although the highly specialized bracts of Himalayan 'glasshouse' plants are thought to be an adaptive morphology that allows the plant to reproduce in harsh alpine environments, little information appears to exist concerning the benefit of these bracts for the pollinating insects. In this study, we examine whether the semi-translucent bracts of Rheum alexandrae, a giant herb endemic to the alpine zone of the Hengduan Mountains in south-western China, enhance the reproductive fitness of its pollinator. Rheum alexandrae depends mainly on mutualistic seed-consuming Bradysia flies for pollination. Bracts increased interior temperature on sunny days, acted as a buffer against fluctuating air humidity, greatly decreased the intensities of ultraviolet B/C radiation and provided shelter from strong winds. Bract removal significantly decreased adult oviposition and offspring performance of pollinators during flowering and fruiting, respectively. Our results indicate that the bracts of R.alexandrae enhance the reproductive fitness of its pollinating seed-consuming mutualist in alpine environments, which may be attributed to the positive effects of the bracts on the interior microenvironment. © 2015 The Linnean Society of London.

Li G.-F.,Yunnan Forestry Technological College | Wang C.-M.,Mojiang County Nationality Middle School | Li H.-F.,Kunming Nengxun Science and Technology Ltd Company | Hou Y.-P.,Yunnan Forestry Technological Promotion Station
Zootaxa | Year: 2015

Oxyporus (Oxyporus) bingshengae sp. nov. in Yunnan, China, is described. Color images of the habitus and aedeagus of the new species are included. A key to the species of Oxyporus of Yunnan is provided. Copyright © 2015 Magnolia Press.

Du H.-Y.,Changchun University of Science and Technology | Wei Z.-P.,Changchun University of Science and Technology | Sun L.-J.,Yunnan Forestry Technological College | Chu X.-Y.,Changchun University of Science and Technology | And 5 more authors.
Chinese Optics | Year: 2013

Mn-doped ZnS nanoparticles were prepared by the Sol-Gel process in this paper, and the influence of doping ion concentration on crystal structures and luminescent properties of ZnS:[KG-*3/5]Mn nanoparticles was discussed. The structures of the samples were characterized by an X-ray diffractometer(XRD). The results show that the as-prepared ZnS:[KG-*3/5]Mn nanoparticles belong to the cubic sphalerite structure. The parvafacies do not occur when Mn2+-doping concentration reaches 6% and the average particle sizes of nanoparticles decrease with the increase of doping concentration. Photoluminescence(PL) spectroscopy and fluorescence spectrum results show that the emission wavelengths of ZnS:[KG-*3/5]Mn nanoparticles around 590 nm can be adjusted by changing the concentration of the ions. In addition, the influence of temperature on morphology and luminescent properties of nanoparticles was studied. The result observed from a high resolution transmission electron microscopy(HRTEM) shows that the average particle sizes of ZnS:[KG-*3/5]Mn samples increase to about 20 nm after ageing for 1 h at the temperature of 50 °C. The heating ageing is beneficial to Mn2+ fluorescence produced at 590 nm for ZnS:[KG-*3/5]Mn nanoparticles.

Zhang S.,Chinese Academy of Forestry | Kong X.,Chinese Academy of Forestry | Ze S.,Bureau of Forest Pest Control and Quarantine of Yunnan Province | Wang H.,Chinese Academy of Forestry | And 3 more authors.
Insect Biochemistry and Molecular Biology | Year: 2016

Pheromone-binding proteins (PBPs) play an important role in the recognition of pheromones by insects. However, the abilities of these PBPs to discriminate pheromone components and recognize the isomers are unclear. Dendrolimus houi and Dendrolimus kikuchii are two sympatric coniferous pests whose pheromones have cis-trans isomers. We used these insect species to detect the precise recognition abilities of PBPs. The four PBPs examined showed male-biased antenna-intensive expression patterns, whereas PBP1 showed higher expression than PBP2 in the antenna. DhouPBP1 only bound to a minor interspecific pheromone component, whereas DhouPBP2 bound to all three intraspecific components and another minor interspecific component. DkikPBP1 and DkikPBP2 could recognize all three intraspecific components with affinities negatively correlated with their ratios, and they bound to interspecific pheromones with affinity that was positively correlated with the ratios. The four PBPs have different cis-trans isomer discrimination abilities, i.e., DhouPBP1 and DkikPBP1 could not discriminate the two cis-trans isomer pairs of pheromones from the two species, whereas DhouPBP2 could discriminate between both pairs, and DkikPBP2 could only discriminate one pair. Overall, PBPs from D. houi and D. kikuchii use different strategies to help the moths to discriminate the intra- and interspecific pheromone components. Our work will contribute to better understanding of the sex pheromone recognition mechanism in these two sister species of moths and provide insights into more effective management practices of these pest species. © 2016 Elsevier Ltd.

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