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Cai Y.-F.,Yunnan Academy of Agricultural Sciences | Cai Y.-F.,Yunnan Flower Breeding Key Laboratory | Cai Y.-F.,Yunnan Flower Research and Development Center | Li S.-F.,Yunnan Academy of Agricultural Sciences | And 8 more authors.
Botanical Studies

Background: Rhododendron is one of the most well-known alpine flowers. In order to identify performances relating to Rhododendron's natural habitats we investigated the leaf anatomical structures and photosynthetic characteristics of R. yunnanense, R. irroratum and R. delavayi, which showed different responses after being transplanted into a common environment. Results: When compared with R. irroratum and R. delavayi, R. yunnanense had lower leaf dry mass per unit area (LMA) and larger stomata, but smaller stomatal density (SD) and total stomata apparatus area percent (At), lower stomatal conductance (Gs), transpiration rate (Tr), light compensation point (LCP), light saturation point (LSP), light-saturated photosynthetic rate (Amax) and leaf nitrogen content per unit area (Na). LMA was positively correlated with Amax and maximum rates of carboxylation (Vcmax). However, leaf N content was not significantly correlated with Amax. Thus, the variation in leaf photosynthesis among species was regulated largely by changes in LMA, rather than the concent of nitrogen in leaf tissue. Conclusions: R. yunnanense plants are vulnerable to moisture and light stress, while R. irroratum and R. delavayi are better suited to dry and high radiation environments. The present results contribute to our understanding physiological trait divergence in Rhododendron, as well benefit introduction and domestication efforts for the three species of Rhododendron studied in this work. © 2014 Cai et al. Source

Zhang Y.P.,Yunnan Agricultural University | Zhang Y.P.,Yunnan Academy of Agricultural Sciences | Zhang Y.P.,Yunnan Flower Breeding Key Laboratory | Zhang Y.P.,Yunnan Flower Engineering Center | And 27 more authors.
Acta Horticulturae

In this study, callus cultures derived from in vitro scales of Oriental lily 'Casa Blanca' susceptible to Fusarium oxysporum f. sp. lilii were successfully used for in vitro selection for developing resistance to this pathogenic fungus. The suitable induction callus medium is a MS supplemented with a hormone combination of TDZ 0.05 mg L-1 and Picloram 2 mg L-1. The callus was added to different concentrations of culture filtrate of F. oxysporum to determine appropriate culture filtrate concentration to be used for disease-resistant selection. And this stock is feasible as selective agent for screening for F. oxysporum wilt resistance. Resistant cell lines were selected by culturing calli on MS medium supplemented with growth regulators and containing 80% concentrations of culture filtrate of F. oxysporum. 7.0% calli regenerated shoots on MS medium supplemented with different concentrations of naphthalene acetic acid (NAA) and benzyladenine (BA). Morphologically, the selected regenerants could be grouped in two kinds of mutants. It is certificated the selected regenerants were mutated through measuring leaf length, leaf width, leaf index and chromosome analysis. The selected clones were moderate resistant to Fusarium bulb rot. Source

Peng L.-C.,Yunnan Academy of Agricultural Sciences | Peng L.-C.,Supervision and Testing Center for Flowers of Ministry of Agriculture Kunming | Peng L.-C.,Yunnan Flower Breeding Key Laboratory | Peng L.-C.,Yunnan Flower Research and Development Center | And 20 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal

The plant regeneration system of Ormosia fordiana Oliv. was studied with the pretreatment and germination induction of seed, multiplication and hardening of adventitious bud, rooting of the shoot. The results showed that the best seed germination medium was MS (Read)+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1 with 100% of germination rate, and the best multiplication medium was Read+6-BA 1.0-1.5 mg·L-1+KT 0.1 mg·L-1+NAA 0.1 mg·L-1 which has more than twenty-three multiplication buds in each bottle. Read+6-BA 0-0.5 mg·L-1+ NAA 0.1 mg·L-1 was the best hardening medium. The best rooting medium was Read+IAA 1.0 mg·L-1 with 90.54% of the rooting rate. The research would promote the reproductive efficiency of Ormosia fordiana Oliv.. It improved the maturity and application of large-scale seedling technology, and had the vital significance on development and application of Ormosia fordiana Oliv.. Source

Yan H.,Yunnan Academy of Agricultural Sciences | Yan H.,Yunnan Flower Breeding Key Laboratory | Zhang H.,Yunnan Academy of Agricultural Sciences | Zhang H.,Yunnan Flower Breeding Key Laboratory | And 19 more authors.

Rosa chinensis 'Pallida' (Rosa L.) is one of the most important ancient rose cultivars originating from China. It contributed the 'tea scent' trait to modern roses. However, little information is available on the gene regulatory networks involved in scent biosynthesis and metabolism in Rosa. In this study, the transcriptome of R. chinensis 'Pallida' petals at different developmental stages, from flower buds to senescent flowers, was investigated using Illumina sequencing technology. De novo assembly generated 89,614 clusters with an average length of 428. bp. Based on sequence similarity search with known proteins, 62.9% of total clusters were annotated. Out of these annotated transcripts, 25,705 and 37,159 sequences were assigned to gene ontology and clusters of orthologous groups, respectively. The dataset provides information on transcripts putatively associated with known scent metabolic pathways. Digital gene expression (DGE) was obtained using RNA samples from flower bud, open flower and senescent flower stages. Comparative DGE and quantitative real time PCR permitted the identification of five transcripts encoding proteins putatively associated with scent biosynthesis in roses. The study provides a foundation for scent-related gene discovery in roses. © 2014 . Source

Yan H.,Yunnan Academy of Agricultural Sciences | Yan H.,Yunnan Flower Breeding Key Laboratory | Zhang H.,Yunnan Academy of Agricultural Sciences | Zhang H.,Yunnan Flower Breeding Key Laboratory | And 10 more authors.
Molecular Biology Reports

Rose fragrances play an important role in attracting pollinators and commercial value. However, some genes involved in rose floral scent metabolism are less well understood. Here, wild-type scented rose (WR) and its spontaneous non-scented mutant rose (MR) were analyzed. SPME-GC/MS analysis showed that relative content of 1-ethenyl-4-methoxy-benzene represented was significantly different between WR and MR. We have isolated an EST encoding a MYB family of transcription factor from SSH libraries of the two roses in the previous studies, and designated RhMYB1. In the study, the full-length cDNA of RhMYB1 was identified and characterized by rapid amplification of cDNA ends (RACE). The RhMYB1 full-length cDNA was 1,125 bp containing an 882 bp open reading frame, which encodes a precursor protein of 294 amino acids. Sequence alignments revealed that RhMYB1 shared high similarity with other plants R-type MYB, and RhMYB1 contained a DNA binding domain. Northern blot analysis revealed that RhMYB1 was expressed specifically in flower petal, moreover, the expression level of RhMYB1 in WR increased along with scent emission, and decreased when the scent emission decreased. It is suggested that RhMYB1 might be a putative identification of gene involved in the biosynthesis of rose scent. © 2010 Springer Science+Business Media B.V. Source

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