Time filter

Source Type

Zhu G.Q.,Yunnan Agricultural University | Zhu G.Q.,Yunnan Engineering Research Center for Japonica Hybrid Rice | Tan X.L.,Yunnan Agricultural University | Tan X.L.,Yunnan Engineering Research Center for Japonica Hybrid Rice | And 20 more authors.
Euphytica | Year: 2015

Cytoplasmic male sterile (CMS) lines varied at pollen abortive phenotypes (PAP) have been used effectively for production of hybrid seeds in rice. To investigate the relationship between PAP of CMS lines and CMS-specific mitochondrial genes or structures in rice, CMS lines varied at PAP developed on 18 cytoplasmic sources were examined with seven PCR primers specific to three CMS-specific mitochondrial regions. The 18 CMS lines were classified into stained abortive (SA) and typical abortive (TA) types according to their PAP. Two CMS cytoplasms-specific PCR fragments, by which genotypes between the CMS lines and their maintainers were distinguished, were identified with primer LD29 or LD30. Furthermore, it was discovered that phenotypes of SA-CMS and TA-CMS were related to CMS-specific mitochondrial genes or structures, LD-orf310, atp6-orf79-like, and orf288-like structures. The results suggested that CMS phenotypes were mainly controlled by cytoplasmic genes, and it could be speculated that the SA-CMS phenotype was related to LD-orf310 and atp6-orf79-like structure, while TA-CMS phenotype was related to WA352. © 2015 Springer Science+Business Media Dordrecht

Discover hidden collaborations