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Wang R.,Henan Agricultural University | Shi H.,Henan Agricultural University | Zhou J.,Beijing Cigarette Factory | Bai R.,Beijing Cigarette Factory | And 5 more authors.
Acta Tabacaria Sinica | Year: 2014

Samples of Binchuan burley tobacco, Dazhou sun-cured tobacco and Baofeng flue-cured tobacco were collected to investigate changes in TSNAs contents during storage and their response to different temperature treatment. Results showed that both total and individual TSNAs contents in both burley and sun-cured tobacco increased with increase of storage time. The most significant increase occurred in high temperature months, for example, from middle of April until August, especially for NNN in burley tobacco. Both total and individual TSNAs contents in flue-cured tobacco which had less nitrate contents were very low and showed non-significant changes after 45°C treatment. While both total and individual TSNAs contents in burley tobacco and sun-cured tobacco were much higher and increased rapidly after the treatment of 45°C, with nitrate decreasing slightly and nitrite rising significantly.


Zhou H.,Henan Agricultural University | Shi H.,Henan Agricultural University | Xu F.,Yunnan Dali Tobacco Company | Sun J.,Yunnan Dali Tobacco Company | And 6 more authors.
Acta Tabacaria Sinica | Year: 2013

Effect of environmental humidity and temperature during air-curing on contents of neutral aroma components and leaf quality was investigated using burley tobaccos harvested in Binchuan and Yunlong, where climatic conditions are distinctively different. Results showed that difference in temperature between the two locations was not significant, while difference in humidity was significant. Humidity in Yunlong was higher than in Binchuan by 4.42-16.54 percentage point in the whole curing period. The time of curing leaf in Binchuan was 9 days shorter than in Yunlong. Leaf quality cured in Yunlong was better than that cured in Binchuan with deeper color, thinner and looser body and more oil. Total aroma components content of leaves cured in Yunlong were significantly higher than that cured in Binchuan. Total aroma compounds in upper leaves and middle leaves were increased by 31.26% and 34.38% respectively. Content of carotenoid catabolities was increased by 27.55% and 29.75% respectively. Leaves cured in Yunlong had significantly less contents of residual chlorophyll and carotenoids. Sensory evaluation indicated that Yunlong cured tobacco had higher aroma quantity and less irritation and better quality, indicating that humidity and leaf quality was correlated.


Li Q.-Q.,China Agricultural University | Wu L.-J.,China Agricultural University | Liu W.,China Agricultural University | Cao J.-L.,Yunnan Dali Tobacco Company | And 3 more authors.
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | Year: 2012

In the present study, sucrose was used as a chiral selector to detect the molar fraction of R-metalaxyl and S-ibuprofen due to the UV spectral difference caused by the interaction of the R-and S-isomer with sucrose. The quantitative model of the molar fraction of R-metalaxyl was established by partial least squares (PLS) regression and the robustness of the models was evaluated by 6 independent validation samples. The determination coefficient R 2 and the standard error of calibration set (SEC) was 99.98% and 0.003 respectively. The correlation coefficient of estimated value and specified value, the standard error and the relative standard deviation (RSD) of the independent validation samples was 0.9998, 0.0004 and 0.054% respectively. The quantitative models of the molar fraction of S-ibuprofen were established by PLS and the robustness of models was evaluated. The determination coefficient R 2 and the standard error of calibration set (SEC) was 99.82% and 0.007 respectively. The correlation coefficient of estimated value and specified value of the independent validation samples was 0.9981. The standard error of prediction (SEP) was 0.002 and the relative standard deviation (RSD) was 0.2%. The result demonstrates that sucrose is an ideal chiral selector for building a stable regression model to determine the enantiomeric composition.


Sun H.,Henan Agricultural University | Zhou H.,Henan Agricultural University | Wang R.,Henan Agricultural University | Song Y.,Henan Agricultural University | And 4 more authors.
Acta Tabacaria Sinica | Year: 2014

Different improved TN86 varieties with different screening standards for identifying nicotine converter and non-converters were compared for economic character, nicotine conversion ratio, and content of TSNAs. Results showed that the improved variety 1(PNC<2.5) with nicotine conversion ratio of less than 2.5% as benchmark was more stable with ratio of upper tobacco 37.7% lower than conventional breed and 38.3% lower for middle part tobacco. Total content of TSNAs was 17.9% lower than conventional breed, and yield, output value and the proportion of middle and superior tobacco were better than other improved varieties with the lowest variable coefficient. The separations of nicotine conversion characters of improved variety 2(PNC<3) and improved variety 3(PNC<5) were significant, and the content of TSNAs was higher. Generally speaking, there was reliable difference between converter and no-converter with nicotine conversion ratio of 2.5%. Choosing tobacco with PNC<2.5 can ensure lower ratio of appearance of converted plant, and more effectively reduce formation and accumulation of nornicotine and TSNAs, thereby improving aroma quality.


Zhang G.,Henan Agricultural University | Shi H.,Henan Agricultural University | Yang X.,Sichuan Dazhou Municipal Tobacco Company | Sun J.,Yunnan Dali Tobacco Company | And 2 more authors.
Acta Tabacaria Sinica | Year: 2015

Field experiments with burley tobacco and flue-cured tobacco material were set up in Dazhou tobacco growing area of Sichuan and Dali tobacco growing area of Yunnan to explore the effect of curing methods on the contents of neutral aroma substances in tobacco. Matured tobacco leaves of the same tobacco type were subjected to flue-curing and air-curing separately, and contents of neutral aroma substances were measured after curing. Results showed that contents and proportions of neutral aroma substances in cured leaves with different curing methods displayed significant difference while their components were the same. The air-curing was unfavorable for the accumulation of aroma substances and neophytadiene in flue-cured tobacco, but it could significantly increase the contents of solanone and Maillard reaction products in flue-cured tobacco. The flue-curing was unfavorable for the accumulation of solanone, linalool, geranyl, dihydroactinidiolided and famesyl aeclonr in burley tobacco, but good for the accumulation of neophytadiene, dehydrogenation, ketoisophorone, megastigmatrienone, solavetivone and aromatic amino acid degradation products. Under air-curing condition, flue-cured tobacco showed little of its due characteristics with decreased aroma quality and sweetness and enhanced irritation; under flue-curing condition, burley tobacco presented little of its burley style with improved aroma quality and sweetness and less irritation. ©, 2015, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.

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