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Qu K.-X.,Northwest University, China | Qu K.-X.,CAS Kunming Institute of Zoology | Qu K.-X.,Yunnan Academy of Grassland and Animal Science | Nguyen S.N.,CAS Kunming Institute of Zoology | And 6 more authors.
African Journal of Biotechnology | Year: 2012

Mithun or gayal (Bos frontalis) is endemic to the Gaoligongshan Mountains and Drung River Basin in Yunnan, China; a rare and endangered Bos species. To evaluate the genetic diversity and bottleneck effect of Yunnan mithun population, we screened 16 bovine microsatellite loci of Yunnan mithun (N = 34) to provide genetic information for its conservation strategies and breeding programmes. A total of 126 microsatellite alleles and large allele size variance were detected. All loci displayed low genetic diversity with overall mean of Na = 7.88, polymorphism information content (PIC) = 0.60, and H E = 0.63, which were the lowest among the beef cattle breeds. Despite the smaller population size, the normal L-shaped distribution of allelic frequencies without any mode-shift indicate the absence of genetic bottleneck in Yunnan mithun population, suggesting that Yunnan mithun population might moderately have underwent gene introgression from zebu and yellow cattle. © 2012 Academic Journals. Source


Qu K.-X.,Northwest University, China | Qu K.-X.,CAS Kunming Institute of Zoology | He Z.-X.,Yunnan Academy of Grassland and Animal Science | Hao R.-J.,Northwest University, China | And 3 more authors.
Genetics and Molecular Research | Year: 2014

The BM2113 locus was amplified in Yunnan mithun (Bos frontalis) from the southwest mountains of China. It showed a high degree of polymorphism with a total of 12 alleles. The 121-bp polymorphic allele of the BM2113 locus that accounted for 37.1% of homozygotes was the predominant allele with a frequency of 58.57%, identified as mithun-specific for Bos species in Yunnan mithun. The polymorphism information content value was high with a mean of 0.6170, the expected and observed heterozygosity was moderate with values of 0.6427 and 0.6000, respectively, and the BM2113 locus was under Hardy-Weinberg equilibrium (P = 0.2897) in the Yunnan mithun population. This study elucidated the genetic diversity, multi-origin, specific alleles, and characterization of mithun. © FUNPEC-RP. Source


Qu K.-X.,Northwest University, China | Qu K.-X.,Yunnan Academy of Grassland and Animal Science | Qu K.-X.,CAS Kunming Institute of Zoology | Huang B.-Z.,Yunnan Academy of Grassland and Animal Science | And 4 more authors.
Russian Journal of Genetics | Year: 2012

cattle (1/2 Brahman, 1/4 Murray Grey and 1/4 Yunnan Yellow cattle) has been inter se breeding since 1980s. Genetic diversity of BMY cattle was extensively investigated using 16 microsatellite markers. A total of 130 microsatellite alleles and high allele size variance were detected. All loci displayed high genetic diversity with overall mean of Na = 8. 13, PIC = 0. 7224 and He = 0. 7666, which were higher than those of many other beef breeds. The allele-sharing neighbour-joining tree clearly displayed the new genotypic combinations and the minglement from both BMY cattle and Brahman. The results provided the genetic information to match the standards of new beef breed in South China. © 2012 Pleiades Publishing, Ltd. Source


He Z.-X.,Yunnan Academy of Grassland and Animal Science | He Z.-X.,Yunnan Beef Engineering and Technological Research Center | Huang M.-F.,Yunnan Academy of Grassland and Animal Science | Wang A.-K.,Yunnan Academy of Grassland and Animal Science | And 6 more authors.
Journal of Animal and Veterinary Advances | Year: 2014

The present study was aimed to investigate the patterns of plasma hormones including oestradiol-17(3 (E2), Progesterone (P4), Follicle-Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Testosterone (T) profiles in Mithun bulls (Bos frontalis) kept together with females under a semi-management. Blood plasmas of 16 Mithun bulls at 1, 2, 3, 4 and 8 years were collected by jugular venipuncture, respectively. Plasma E2, P4, FSH, LH and T were measured by Radioimmunoassay (RIA). As plasma T levels were increasing with age and reached a peak at 8 years (11.39±0.26ng inL-1) (p<0.01) with significant positive correlation between plasma T concentration and bull ages (r = 0.933, p<0.01), body weight (r = 0.726, p<0.01). Plasma P4 concentration was gradually decreasing with age and the peak of P4 concentration was observed at 1 year (0.43±0.14 ng mL-1) while it stayed the lowest level at 8 years (0.13±0.02 ng mL-1) (p<0.05). The plasma P4 concentrations had a significant negative relationship with age (r = -0.519, p<0.05), body weight (r = -0.468, p<0.05) in Mithun bulls. Also the plasma E2, FSH and LH concentrations trended to decrease with age (p>0.05). The plasma FSH concentration had a significant positive correlation with P4 concentration (r = 0.621, p<0.01), similar to a positive correlation between plasma LH and P4 concentrations (r = 0.259, p>0.05). The negative correlation were found between the concentrations of plasma P4 and E2 (r = -0.126, p>0.05) and between T and P4 levels (r = -0.431, p = 0.057), respectively. © Medwell Journals, 2014. Source


Zhao G.,Yunnan Academy of Grassland and Animal Science | Zhao G.,Nanjing Agricultural University | Yu M.,Yunnan Academy of Grassland and Animal Science | Cui Q.-W.,Nanjing Agricultural University | And 7 more authors.
Genetics and Molecular Research | Year: 2013

We investigated a possible association between bovine Toll-like receptor 4 (TLR4) and resistance to tick infestation in 103 cattle, including BMY cattle (1/2 Brahman, 1/4 Murray Grey, and 1/4 Yunnan Yellow cattle), Brahman, and Red Angus grazing on improved pasture. The tick infestation weight and number of Rhipicephalus microplus and the blood histamine concentration were measured and compared with those of 32 Chinese Holsteins and 30 Simmentals. A 228-bp fragment was amplified and sequenced to analyze the polymorphisms of the TLR4 gene. After SSCP and sequencing analysis, 4 SNPs, i.e., 535(A>C), 546(T>C), 605(T>A), and 618(G>C), were identified, corresponding to GenBank accession Nos. AY297041 and NW_003104150; the latter two SNPs caused Leu→Gln and Gln→His substitutions, respectively. Genotype AA was completely predominant in the Chinese Holstein and Simmental; genotypes AA and AB were detected in Red Angus, while genotypes AA, AB, BB, and BC were detected in Brahman and in BMY cattle. A negative correlation was identified between blood histamine concentration and number of tick infestation; in BMY cattle this negative association was significant. The tick infestation in cattle with genotype BB was significantly lower than in those with genotype AA. Blood histamine concentration in cattle with genotype BB was significantly higher than in those with genotype AA. The TLR4 gene mutation could affect the blood histamine level and activate the immune reaction after tick infestation. Allele B has potential as a molecular marker for tick-resistance originated from Zebu cattle for use in cattle breeding programs. © FUNPEC-RP. Source

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