Yi C.-H.,Yunnan Academy of Forestry |
He Q.-J.,Southwest forestry University
Entomological News | Year: 2013
Two new species of Epilachna Dejean from Yunnan, China, are described: Epi lach na nodaodea sp. nov and E. lingulatus sp. nov.
Zhu J.-Y.,Southwest forestry University |
Zhang L.-F.,Southwest forestry University |
Ze S.-Z.,Yunnan Academy of Forestry |
Wang D.-W.,Southwest forestry University |
Yang B.,Southwest forestry University
Journal of Insect Physiology | Year: 2013
Odorant binding proteins (OBPs) contribute to the remarkable sensitivity of the insect's olfactory system and play an important role in insect chemical communication. In this study, we identified 11 putative cDNAs encoding OBPs (namely SexiOBP1-11) from the antennal full length cDNA library of the beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) and examined their expression profiles in different adult body tissues (antennae, heads, thoraxes, abdomens, legs and wings) by real-time quantitative PCR (qPCR). All SexiOBPs had the characteristic typical features of the OBP family, with the exception of SexiOBP11, which lacked the predicted signal peptide sequence at the N-terminus. qPCR revealed that all of these genes were highly transcribed in the antennae. SexiOBP1-4 and SexiOBP10 were dominantly restricted to antennae. Within antennae, SexiOBP2-4 and SexiOBP10 exhibited female-biased expression patterns, while the expression of SexiOBP7 was male-biased, indicating that they might be involved in interacting with sex pheromones. In general, these OBPs were mainly expressed in chemosensory-specific tissues, although some displayed non-chemosensory or ubiquitous tissue expression. The data is helpful for further determining the potential physiological functions of S. exigua OBPs, and paves the way towards a better understanding of the chemosensory perception of this pest, which may help to uncover new targets for behavioral interference used as a control strategy. © 2013 Elsevier Ltd.
Yang Y.,Yunnan Academy of Forestry |
Wang J.,Southwest forestry University
Acta Horticulturae | Year: 2013
Yunnan province has the richest bamboo resources, and most of them are sympodial bamboo. We have done a large experiment on the cultivation of sympodial bamboo and exploitation of bamboo products. This paper summarizes some key techniques concerning the whole process from cultivation to integrated development of sympodial bamboo, which includes the techniques of propagation and seedling growing, silvicultural activities, management techniques, integrated development and utilization. And this paper also analyzed problems existing in the process and some recommendations are put forward.
Li W.,Southwest forestry University |
Tan R.,Southwest forestry University |
Yang Y.-M.,Yunnan Academy of Forestry |
Wang J.,Yunnan Academy of Forestry
Journal of Mountain Science | Year: 2014
Biotic indicators have been widely used to monitor wetland health. However, few studies have explicitly evaluated if plant diversity could serve as a useful community-level indicator of wetland stability, especially when wetlands are confronted with anthropogenic perturbations. Based on three-year record of wetland plant species abundance in Napahai plateau wetland, Shangri-la under the influence of varying anthropogenic perturbation types, our study tests the impact of such perturbations on plant richness and the relationship between ecosystem temporal stability and plant richness, and further assesses the effectiveness of using plant diversity indicator to probe ecosystem temporal stability of Napahai plateau wetland and the potential mechanisms. The results showed that anthropogenic perturbations could have contributed significantly to realistic variation in plant diversity, and further demonstrated that ecosystem temporal stability was positively related to realistic variation in plant diversity. In particular, communities with high levels of diversity might have better capacity to dampen perturbation impacts than communities with low levels of diversity, and statistical averaging could have played an important role in causing greater stability in more diverse communities. Also, asynchrony might have a stabilizing effect on community stability, and diversity could have stabilized communities through both species asynchrony and population stability propagation. Therefore, our results suggest that plant diversity could be used as a useful indicator of the stability conditions of plateau wetland ecosystems confronted with anthropogenic perturbations, and the preservation of plant communities at sufficient abundance and diversity is necessary for maintaining healthy plateau wetlands and for sustaining their essential ecosystem functions and services. © Science Press and Institute of Mountain Hazards and Environment, CAS and Springer-Verlag Berlin Heidelberg 2014.
Yuan Y.,Zhejiang University |
Wang B.,Zhejiang University |
Zhang S.,Zhejiang University |
Zhang S.,Yunnan Academy of Forestry |
And 5 more authors.
Journal of Plant Ecology | Year: 2013
Aims Why invasive plants are more competitive in their introduced range than native range is still an unanswered question in plant invasion ecology. Here, we used the model invasive plant Solidago Canadensis to test a hypothesis that enhanced production of allelopathic compounds results in greater competitive ability of invasive plants in the invaded range rather than in the native range. We also examined the degree to which the allelopathy contributes increased competitive ability of S. canadensis in the invaded range. Methods We compared allelochemical production by S. canadensis growing in its native area (the USA) and invaded area (China) and also by populations that were collected from the two countries and grown together in a 'common garden' greenhouse experiment. We also tested the allelopathic effects of S. canadensis collected from either the USA or China on the germination of Kummerowia striata (a native plant in China). Finally, we conducted a common garden, greenhouse experiment in which K. striata was grown in monoculture or with S. canadensis from the USA or China to test the effects of allelopathy on plant-plant competition with suitable controls such as adding activated carbon to the soil to absorb the allelochemicals and thereby eliminating any corresponding allopathic effects. Important findings Allelochemical contents (total phenolics, total flavones and total saponins) and allelopathic effects were greater in S. Canadensis sampled from China than those from the USA as demonstrated in a field survey and a common garden experiment. Inhibition of K. striata germination using S. canadensis extracts or previously grown in soil was greater using samples from China than from the USA. The competitive ability of S. canadensis against K. striata was also greater for plants originating from China than those from the USA. Allelopathy could explain about 46% of the difference. These findings demonstrated that S. canadensis has evolved to be more allelopathic and competitive in the introduced range and that allelopathy significantly contributes to increased competitiveness for this invasive species. © The Author 2012. Published by Oxford University Press on behalf of the Institute of Botany, Chinese Academy of Sciences and the Botanical Society of China.All rights reserved.
Mao R.,CAS Changchun Northeast Institute of Geography and Agroecology |
Song C.-C.,CAS Changchun Northeast Institute of Geography and Agroecology |
Zhang X.-H.,CAS Changchun Northeast Institute of Geography and Agroecology |
Wang X.-W.,CAS Changchun Northeast Institute of Geography and Agroecology |
Zhang Z.-H.,Yunnan Academy of Forestry
Plant and Soil | Year: 2013
Background and Aims: Increased N availability induced by agricultural fertilization applications and atmospheric N deposition may affect plant nutrient resorption in temperate wetlands. However, the relationship between nutrient resorption and N availability is still unclear, and most studies have focused on leaf nutrient resorption only. The aim of our study was to examine the response of leaf and non-leaf organ nutrient resorption to N enrichment in a temperate freshwater wetland. Methods: We conducted a 7-year N addition experiment to investigate the effects of increased N loading on leaf, sheath and stem nutrient (N and P) resorption of two dominant species (Deyeuxia angustifolia and Glyceria spiculosa) in a freshwater marsh in the Sanjiang Plain, Northeast China. Results: Our results showed that, for both leaf and non-leaf organs (sheath and stem), N addition decreased N resorption proficiency and hence increased litter N concentration. Moreover, the magnitude of N addition effect on N resorption proficiency varied with fertilization rates for D. angustifolia sheaths and stems, and G. spiculosa leaves. However, increased N loading produced inconsistent impacts on N and P resorption efficiencies and P resorption proficiency, and the effects only varied with species and plant organs. In addition, N enrichment increased litter mass and altered litter allocation among leaf, sheath and stem. Conclusions: Our results highlight that leaf and non-leaf organs respond differentially to N addition regarding N and P resorption efficiencies and P resorption proficiency, and also suggest that N enrichment in temperate freshwater wetlands would alter plant internal nutrient cycles and increase litter quality and quantity, and thus substantially influence ecosystem carbon and nutrient cycles. © 2012 Springer Science+Business Media B.V.
Gu Y.,Chinese Academy of Forestry |
Chen F.,Yunnan Academy of Forestry |
Wu H.,Chinese Academy of Forestry
Plant Physiology Communications | Year: 2010
The techniques and measures of tissue culture and rapid propagation of Calamus nambariensis Becc. var. xishuangbannaensis S. J. Pei et S. Y. Chen were carried out from some aspects, including sample preparation, expiant differentiation, bud propagation, root generation and factors affecting test-tube plantlet formation. The results have been showed that the optimum medium for inducing bud differentiation is improved MS medium plus 0.5 mg·L-1 NAA, 0.5 mg·L-1 KT and 0.1 mg·L-1 BA, while adding 1.5-2.0 mg·L-1 IBA to the medium is favorable to root.
PubMed | Yunnan University, Yunnan Academy of Forestry and Southwest forestry University
Type: | Journal: Journal of insect science (Online) | Year: 2015
The oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), is considered a major economic threat in many regions worldwide. To better comprehend flight capacity of B. dorsalis and its physiological basis, a computer-monitored flight mill was used to study flight capacity of B. dorsalis adult females of various ages, and the changes of its flight muscle ultrastructures were studied by transmission electron microscopy. The flight capacity (both speed and distance) changed significantly with age of B. dorsalis female adults, peaking at about 15d; the myofibril diameter of the flight muscle of test insects at 15-d old was the longest, up to 1.56m, the sarcomere length at 15-d old was the shortest, averaging at 1.37m, volume content of mitochondria of flight muscle at 15-d old reached the peak, it was 32.64%. This study provides the important scientific data for better revealing long-distance movement mechanism of B. dorsalis.
PubMed | Zhejiang Agriculture And forestry University, Yunnan University and Yunnan Academy of Forestry
Type: Journal Article | Journal: Journal of economic entomology | Year: 2015
Spodoptera litura F. is one of the most destructive insect pests of many agricultural crops and notorious for developing insecticide resistance. Developing environmental friendly control methods such as novel pheromone and RNAi-related control strategies is imperative to control this pest. In the present study, the full-length cDNA encoding the diapause hormone and pheromone biosynthesis activating neuropeptide (DH-PBAN) was identified and characterized in S. litura. This 809-bp transcript contains a 573-nucleotide ORF encoding a 191-amino acid protein, from which five putative neuropeptides, including PBAN, DH, and -, -, and -subesophageal ganglion neuropeptides, were derived. Phylogenetic analysis showed that both the whole protein and each of the five neuropeptides have high similarities to those of DH-PBANs from other insect orders particularly Lepidoptera. Females treated with TKYFSPRLamide (the active core fragment of PBAN) produced significantly more four types of pheromone compounds (A; B; C; D) than controls. RNA interference by injection of PBAN dsRNA significantly reduced the relative expression levels of this gene in adult females (approximately reduced by 60%). As a consequence, females treated with PBAN dsRNA produced significantly less four types of pheromone compounds (A; B; C; D) than controls. These results suggest that PBAN function in activating sex pheromone biosynthesis and the RNAi of DH-PBAN gene can be induced by the injection of dsRNA into the body cavity in S. litura. This study suggests the possibility of novel pheromone-related pest control strategies based on RNAi techniques.
PubMed | Yunnan University, Yunnan Academy of Forestry and Institute of Yunnan Forestry Inventory and Planning
Type: | Journal: Scientific reports | Year: 2016
Protecting wilderness areas (WAs) is a crucial proactive approach to sustain biodiversity. However, studies identifying local-scale WAs for on-ground conservation efforts are still very limited. This paper investigated the spatial patterns of wilderness in a global biodiversity hotspot - Three Parallel Rivers Region (TPRR) in southwest China. Wilderness was classified into levels 1 to 10 based on a cluster analysis of five indicators, namely human population density, naturalness, fragmentation, remoteness, and ruggedness. Only patches characterized by wilderness level 1 and 1.0km(2) were considered WAs. The wilderness levels in the northwest were significantly higher than those in the southeast, and clearly increased with the increase in elevation. The WAs covered approximately 25% of TPRRs land, 89.3% of which was located in the >3,000m elevation zones. WAs consisted of 20 vegetation types, among which temperate conifer forest, cold temperate shrub and alpine ecosystems covered 79.4% of WAs total area. Most WAs were still not protected yet by existing reserves. Topography and human activities are the primary influencing factors on the spatial patterns of wilderness. We suggest establishing strictly protected reserves for most large WAs, while some sustainable management approaches might be more optimal solutions for many highly fragmented small WAs.