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Yi C.-H.,Yunnan Academy of Forestry | He Q.-J.,Southwest forestry University
Entomological News | Year: 2013

Two new species of Epilachna Dejean from Yunnan, China, are described: Epi lach na nodaodea sp. nov and E. lingulatus sp. nov.

Zhu J.-Y.,Southwest forestry University | Zhang L.-F.,Southwest forestry University | Ze S.-Z.,Yunnan Academy of Forestry | Wang D.-W.,Southwest forestry University | Yang B.,Southwest forestry University
Journal of Insect Physiology | Year: 2013

Odorant binding proteins (OBPs) contribute to the remarkable sensitivity of the insect's olfactory system and play an important role in insect chemical communication. In this study, we identified 11 putative cDNAs encoding OBPs (namely SexiOBP1-11) from the antennal full length cDNA library of the beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) and examined their expression profiles in different adult body tissues (antennae, heads, thoraxes, abdomens, legs and wings) by real-time quantitative PCR (qPCR). All SexiOBPs had the characteristic typical features of the OBP family, with the exception of SexiOBP11, which lacked the predicted signal peptide sequence at the N-terminus. qPCR revealed that all of these genes were highly transcribed in the antennae. SexiOBP1-4 and SexiOBP10 were dominantly restricted to antennae. Within antennae, SexiOBP2-4 and SexiOBP10 exhibited female-biased expression patterns, while the expression of SexiOBP7 was male-biased, indicating that they might be involved in interacting with sex pheromones. In general, these OBPs were mainly expressed in chemosensory-specific tissues, although some displayed non-chemosensory or ubiquitous tissue expression. The data is helpful for further determining the potential physiological functions of S. exigua OBPs, and paves the way towards a better understanding of the chemosensory perception of this pest, which may help to uncover new targets for behavioral interference used as a control strategy. © 2013 Elsevier Ltd.

Gu Y.,Chinese Academy of Forestry | Chen F.,Yunnan Academy of Forestry | Wu H.,Chinese Academy of Forestry
Plant Physiology Communications | Year: 2010

The techniques and measures of tissue culture and rapid propagation of Calamus nambariensis Becc. var. xishuangbannaensis S. J. Pei et S. Y. Chen were carried out from some aspects, including sample preparation, expiant differentiation, bud propagation, root generation and factors affecting test-tube plantlet formation. The results have been showed that the optimum medium for inducing bud differentiation is improved MS medium plus 0.5 mg·L-1 NAA, 0.5 mg·L-1 KT and 0.1 mg·L-1 BA, while adding 1.5-2.0 mg·L-1 IBA to the medium is favorable to root.

Mao R.,CAS Changchun Northeast Institute of Geography and Agroecology | Song C.-C.,CAS Changchun Northeast Institute of Geography and Agroecology | Zhang X.-H.,CAS Changchun Northeast Institute of Geography and Agroecology | Wang X.-W.,CAS Changchun Northeast Institute of Geography and Agroecology | Zhang Z.-H.,Yunnan Academy of Forestry
Plant and Soil | Year: 2013

Background and Aims: Increased N availability induced by agricultural fertilization applications and atmospheric N deposition may affect plant nutrient resorption in temperate wetlands. However, the relationship between nutrient resorption and N availability is still unclear, and most studies have focused on leaf nutrient resorption only. The aim of our study was to examine the response of leaf and non-leaf organ nutrient resorption to N enrichment in a temperate freshwater wetland. Methods: We conducted a 7-year N addition experiment to investigate the effects of increased N loading on leaf, sheath and stem nutrient (N and P) resorption of two dominant species (Deyeuxia angustifolia and Glyceria spiculosa) in a freshwater marsh in the Sanjiang Plain, Northeast China. Results: Our results showed that, for both leaf and non-leaf organs (sheath and stem), N addition decreased N resorption proficiency and hence increased litter N concentration. Moreover, the magnitude of N addition effect on N resorption proficiency varied with fertilization rates for D. angustifolia sheaths and stems, and G. spiculosa leaves. However, increased N loading produced inconsistent impacts on N and P resorption efficiencies and P resorption proficiency, and the effects only varied with species and plant organs. In addition, N enrichment increased litter mass and altered litter allocation among leaf, sheath and stem. Conclusions: Our results highlight that leaf and non-leaf organs respond differentially to N addition regarding N and P resorption efficiencies and P resorption proficiency, and also suggest that N enrichment in temperate freshwater wetlands would alter plant internal nutrient cycles and increase litter quality and quantity, and thus substantially influence ecosystem carbon and nutrient cycles. © 2012 Springer Science+Business Media B.V.

Yang Y.,Yunnan Academy of Forestry | Wang J.,Southwest forestry University
Acta Horticulturae | Year: 2013

Yunnan province has the richest bamboo resources, and most of them are sympodial bamboo. We have done a large experiment on the cultivation of sympodial bamboo and exploitation of bamboo products. This paper summarizes some key techniques concerning the whole process from cultivation to integrated development of sympodial bamboo, which includes the techniques of propagation and seedling growing, silvicultural activities, management techniques, integrated development and utilization. And this paper also analyzed problems existing in the process and some recommendations are put forward.

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