Yunnan Academy of Applied Technology

Kunming, China

Yunnan Academy of Applied Technology

Kunming, China

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Zhou Y.,Yunnan Academy of Applied Technology | Zhou Y.,Kunming Iron and Steel Group Co. | Wang Z.,Yunnan Academy of Applied Technology | Lai Y.,Yunnan Academy of Applied Technology | And 5 more authors.
Xiyou Jinshu Cailiao Yu Gongcheng/Rare Metal Materials and Engineering | Year: 2014

This paper studies the process of developing and producing titanium plate with the 1725mm double stands steckel mill. The main production equipment, technological process and manufacturing technique control of the mill were discussed, as well as heating temperature, rolling temperature, deformation per pass, dimension control, shape control technology were discussed. Based on analysis and discussion, the rolling technical parameter were identified: heating temperature 900°C, finishing temperature 800°C, coiling temperature 620°C; Rolling width 1250 mm, roll curve 1500 mm, roll crown -0.16 mm; Except roller cooling water, mill descaling water, Frit water and laminar cooling water were closed in rolling process; Air cooling after rolling. Under the rolling process parameters of titanium plate's dimension, mechanical properties, microstructure, fracture morphology were emphatically analyzed. The results show that titanium plate produced by this technique has met with National standards.


Gao Z.,CAS Shenyang Institute of Applied Ecology | Gao Z.,University of Chinese Academy of Sciences | Li Y.,CAS Shenyang Institute of Applied Ecology | Li Y.,University of Chinese Academy of Sciences | And 4 more authors.
Applied Biochemistry and Biotechnology | Year: 2015

Transformation approach is a useful tool for the study of gene function, the mechanism of molecular regulation, and increase usefulness of components by reverse genetic approach in plants. In this study, we developed a stable and rapid method for Agrobacterium-mediated transformation of a medicinal plant Chelone glabra L. using leaf explants. Stable transformants were obtained using Agrobacterium tumefaciens strains GV2260 and GV3101 that harbored the binary vector pBI121 and contained the neomycin phosphotransferase gene (NPT II) as a selectable marker and a reporter gene β-glucuronidase (GUS). Putative transformants were identified by kanamycin selection and a histochemical assay. PCR and Southern blot analysis confirmed the integration of the GUS gene into transformed genomes as well as detected stable expression of the β-glucuronidase gene (GUS) by RT-PCR. Resulting transformed plants had morphologically normal phenotypes. This method requires two changes of medium and few leaf explants as well as the transformation efficiency of 2–8 % after 2–3 months of inoculation. This method can provide a quick and economical transformation method for reverse genetic approach to change the secondary metabolic pathway to increase useful components in C. glabra. © 2014, Springer Science+Business Media New York.


Li Y.,CAS Shenyang Institute of Applied Ecology | Li Y.,University of Chinese Academy of Sciences | Gao Z.,CAS Shenyang Institute of Applied Ecology | Gao Z.,University of Chinese Academy of Sciences | And 4 more authors.
Applied Biochemistry and Biotechnology | Year: 2014

In this study, we developed a rapid and efficient method for in vitro propagation and Agrobacterium tumefaciens-mediated transformation of Digitalis purpurea L. (syn. foxglove), an important medicinal plant. Mature leaf explants of D. purpurea were used for 100 % adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 1 mg L-1 thidiazuron (TDZ) (a cytokine) and 0.1 mg L-1 1-naphthaleneacetic acid (NAA) (an auxin). Transformation was achieved by inoculating leaf explants with the A. tumefaciens strains GV2260/pBI121 or GV3101/pBI121. The binary vector pBI121 contained the reporter β-glucuronidase gene (GUS) and kanamycin selection marker nptII. Kanamycin-resistant shoots were regenerated directly on the selection medium 4-6 weeks after co-cultivation. Approximately, 52.2 and 60 % of kanamycin-resistant shoots transformed with Agrobacterium strains GV2260 and GV3101, respectively, showed strong GUS staining by histochemical assay. Furthermore, PCR and Southern blot analysis confirmed the presence of nptII and GUS on the chromosome of the transformed D. purpurea plants, and stable GUS expression was detected in the transformants by RT-PCR analysis. This efficient method of shoot regeneration and genetic transformation of D. purpurea will provide a powerful tool to increase and produce valuable components such as digitoxin, digoxin, and digoxigenin in D. purpurea through improved secondary metabolic pathways via a biotechnological approach. © 2013 Springer Science+Business Media.


Chen L.,Beijing Forestry University | Zhang Z.,Beijing Forestry University | Zha T.,Beijing Forestry University | Mo K.,Beijing Forestry University | And 2 more authors.
New Forests | Year: 2014

Influences of environmental factors on transpiration are interactive. Sensitivities of transpiration responses to both evaporative demand and rainfall under contrasting soil water conditions constitute the physiological basis of the drought tolerance of trees. Such knowledge is practically significant for plantation management, especially for irrigation management. We therefore conducted a 6-year study on the transpiration of a poplar plantation in temperate China to elucidate the existence and pattern of the influence of the soil water over stand transpiration responses to (1) vapor pressure deficit (VPD), the major indicator of air dryness and (2) the rainfall, in terms of total amount and event size. The results showed that the response of plantation transpiration (Ec) to VPD was conditioned by soil moisture. There was a significant difference in the frequency distribution of maximum sap flux under contrasting soil relative extractable water. E c after rainfall of different sizes varied under similar VPD. The increasing occurrences of only large rainfall events led to enhanced total E c during the growing season, but prolonged rainless intervals did not lead to a continuous decrease of E c, suggesting appreciable supplements from the soil water were present to sustain transpiration. In addition, the balance of soil water between replenishment and extraction also conditioned the influence of rainfall over subsequent E c during the respective rainless intervals. Based on the E c responses to VPD and rainfall under different soil moisture levels, irrigation that directly replenishes the deep soil layers in order to alleviate water stress on transpiration during the small-rain event-dominated growing season is an effective and water-saving approach to guarantee trees survival during drought period. © 2014 Springer Science+Business Media Dordrecht.

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