Jiang X.-W.,309th Hospital of Chinese PLA |
Li X.-R.,Yuncheng Central Hospital |
Zhang Y.-P.,309th Hospital of Chinese PLA
International Journal of Clinical and Experimental Medicine | Year: 2015
To investigate the changes of ribbon synapses (RS) number in cochlear hair cells in C57BL/6J mice with age. Basilar membranes within the cochlea of C57BL/6J mice aged 2, 6, 10 and 12 months were harvested (5 mice in each age group). The presynaptic and postsynaptic membranes were subject to double immunohistochemical staining and observed with a laser confocal microscope. The number of RS in each segment of basilar membrane was counted by using 3D reconstruction technique. Compared with 2-month-old mice, reduction of RS number in basilar membrane inside cochlea mainly occurred to the basal turn among C57BL/6J mice aged 6 months. The number of RS in each turn among 10-month-old mice decreased considerably, and such decrease continued in the top turn and middle turn in mice aged 12 months. In contrast, the number of RS in the basal turn increased slightly. Reduction of RS probably takes place in the early stage of C57BL/6J mice presbycusis. Early prevention of presbycusis can be achieved through measures to mitigate the reduction of RS. © 2015, E-Century Publishing Corporation. All rights reserved.
Li T.,Huazhong University of Science and Technology |
Ren J.-H.,Huazhong University of Science and Technology |
Ma Y.-Q.,Huazhong University of Science and Technology |
Lei Y.-R.,Huazhong University of Science and Technology |
And 2 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2012
OBJECTIVE: To investigate the possible association between transforming growth factor-beta1(TGF-β1) -509C/T polymorphism and susceptibility to non-small cell lung cancer(NSCLC) in Chinese population. METHODS: The associations of TGF-β1 -509C/T polymorphism with the susceptibility and clinical tumor characteristics of 210 NSCLC cases and 208 controls matched with sex and age were investi gated. The polymorphism were detemined by using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Logistic regression analysis was employed to assess the associations between genotypes and the risk of NSCLC, adjusting for multiple confounding factors. RESULTS: The TGF-β1-509C/T T allele had a significantly increased risk for NSCLC (P=0.001; OR: 1.617; 95%CI: 1.210-2.161) as compared with the C allele. The T-allele carries(CT or TT genotypes) had a significantly increased risk for NSCLC(P=0.007, OR=2.297, 95%CI: 1.250-4.219) when compared with CC genotype. People who smoked heavily had a increased NSCLC risk(P=0.021, OR=1.783, 95%CI: 1.089-2.918). CONCLUSION: It's suggested that the T allele of TGF-β1-509C/T was associated with the increased risk for NSCLC and the TGF-β1-509C/T polymorphism may be a important marker for susceptibility to NSCLC in Chinese population.
Tian L.-L.,Shihezi University |
Tian L.-L.,Ophthalmic Center |
Ren B.,Ophthalmic Center |
Gao X.-W.,Ophthalmic Center |
And 6 more authors.
International Journal of Ophthalmology | Year: 2014
AIM To investigate the effect of intravitreal injection administered sorafenib, a multikinase inhibitor, in a rat model of oxygen-induced retinopathy (OIR). METHODS Seven-day-old Spr ague-Daw ley rats (72=144) were randomly assigned to six groups. Group A received normal partial oxygen pressure and groups B, C, D, E and F were exposed to hy peroxia (75 ±2)% from postnatal yd (P7) to Pi 2 to induce retinopathy of prematurity . The rats in groups C, D, E and F were received intravitreal injections of either vehicle (DMSO) or sorafenib atPi2 (5, 20 and8o \ig, respectively). Then they returned to normoxia after Pi 2. The retinas were whole-mounted and imaged with a confocal microscopy. The vascular branching points were counted to quantify neovascularization at Pi 7 . Cross-sections of the retina were stained with hematoxylin and eosin (HE). The nuclei of new vessels breaking the internal limiting membrane were counted to quantify the proliferative neovascular response. RESULTS The retinal vessel in groups B and C turned into tortuosity and a great deal of neovascularization were observed. Sorafenib-treated rats had significantly less neovascularization as compared with vehicle-treated and control rats in a dose dependent manner (P
Zhang Q.,Yuncheng Central Hospital |
Zhang Q.,Soochow University of China |
Huang C.,Soochow University of China |
Meng B.,Soochow University of China |
And 3 more authors.
International Journal of Molecular Sciences | Year: 2012
Ghrelin, a 28-amino acid peptide, is mainly secreted by the stomach. Ghrelin has been shown to have neuroprotective effects. However, whether ghrelin protects the spinal cord from ischemia/reperfusion (I/R) injury is unknown. To investigate this, 60 rats were randomly divided into three different groups: the sham group (n = 20), the vehicle group (n = 20), and the Ghrelin group (100 μg/kg, n = 20). Rats were sacrificed 12, 24, 48 and 72 h after ischemia. After the evaluation of neurologic function (48 h), the spinal cords were immediately removed for the determination of myeloperoxidase (MPO) activity (12-72 h). Apoptosis was quantitatively measured using the terminal transferase UTP nick end-labeling (TUNEL) method (24 h). The expression of bax and bcl-2 were evaluated by Western blot analysis (1 h), and GHSR-1a mRNA expression was detected using reverse transcriptase polymerase chain reaction (24 h). The neurological motor function was evaluated by 'Tarlov's score'. The neurologic outcomes in the ghrelin-group were significantly better than those in the vehicle group (p < 0.05). Serum tumor necrosis factor (TNF-α) levels were assessed in the peripheral venous blood. Ghrelin decreased the serum TNF-α levels and ameliorated the down regulation of spinal cord MPO activity. The expression of ghrelin receptors (GHSR-1a) in the rat spinal cord was decreased by I/R injury and increased by ghrelin. Ghrelin reduced the TUNEL-positive rate. Greater bcl-2, HSP27, HSP70, and attenuated bax expression were observed in the ghrelin-treated rats. Our results suggest that ghrelin administration may inhibit spinal I/R injury. Moreover, the improvement of neurologic function in rats was increased after the ghrelin treatment. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
Qin Z.,Yuncheng Central Hospital |
Chen H.,Soochow University of China |
Bin M.,Soochow University of China |
Tiansi T.,Soochow University of China |
Huilin Y.,Soochow University of China
Chinese Journal of Traumatology - English Edition | Year: 2014
Objective Autophagy is involved in several neurodegenerative diseases and recently its role in acute brain injury has won increasing interest. Spinal cord injuries (SCIs) often lead to permanent neurological deficit. Therefore, in this study, we examined the profiles of autophagy-linked proteins (MAP-LC3) after SCI to investigate whether the expression of autophagy contributes to neurological deficit after SCI Methods Adult female Sprague-Dawley rats were used and randomly divided into control and SCI groups. All the rates received laminectomy at T8-T10 level. Those in the SCI group received additional exposure of the dorsal surface of the spinal cord, followed by a weight-drop injury. Thereafter we investigated the expression levels of MAP-LC3, beclin-1, Cathepsin D and the beclin-1-binding protein bcl-2 by western blot analysis at 12 h, 24 h, 3 d, 7 d, 21 d and 28 d. One-way ANOVA with Tukey post hoc test was used to compare data between groups. Results We observed significant increase in the level of LC3 (LC3-II/LC3-I) at 3 d and 7 d after SCI when compared with the sham group. While the level of beclin-1 and ratio of beclin-1/bcl-2 was found to have increased from 12 h to 24 h after injury. Cathepsin D expression was also elevated at 7 d (P < 0.01). Conclusion Based on the above mentioned data, we proposed that autophagy plays a role in the manifestation of cell injury following SCI. © 2014 Daping hospital and the Research Institute of Surgery of the Third Military Medical University.