Yuncheng Central Hospital

Yuncheng, China

Yuncheng Central Hospital

Yuncheng, China
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Objective To explore the clinical significance of immunohistochemistry (IHC) for determining the primary site of adenocarcinoma cells in pleural effusion cell block. Methods One hundred and eight cases of pleural effusion diagnosed as adenocarcinoma were collected by using liquid-based cytology from January 2013 to June 2016 in Yuncheng Central Hospital. Sediment cells were gathered through centrifuging and paraffin block was made, then 94 cases were selected for IHC detection. Results Among 94 cases of adenocarcinoma diagnosed by using liquid-based cytology, 92 cases were still diagnosed as adenocarcinoma by IHC, including 70 cases of lung source, 2 cases of breast, 6 cases of ovary, 8 cases of alimentary canal, 1 case of peritoneal serouspapillary carcinoma, and 5 cases of unknown source. The other 2 cases were diagnosed as mesothelial cells and mesothelial tumors respectively. Conclusion Pleural effusion cells block and multiple IHC can improve the tumor cell detection for the judgement of the benign and malignant tumor cells and differential diagnosis to find the primary site of tumors.


Zhang Q.,Yuncheng Central Hospital
Chinese journal of traumatology = Zhonghua chuang shang za zhi | Year: 2014

OBJECTIVE: Autophagy is involved in several neurodegenerative diseases and recently its role in acute brain injury has won increasing interest. Spinal cord injuries (SCIs) often lead to permanent neurological deficit. Therefore, in this study, we examined the pro?les of autophagy-linked proteins (MAP-LC3) after SCI to investigate whether the expression of autophagy contributes to neurological deficit after SCI.METHODS: Adult female Sprague-Dawley rats were used and randomly divided into control and SCI groups. All the rates received laminectomy at T8-T10 level. Those in the SCI group received additional exposure of the dorsal surface of the spinal cord, followed by a weight- drop injury. Thereafter we investigated the expression levels of MAP-LC3, beclin-1, Cathepsin D and the beclin-1-binding protein bcl-2 by western blot analysis at 12 h, 24 h, 3 d, 7 d, 21 d and 28 d. One-way ANOVA with Tukey post hoc test was used to compare data between groups.RESULTS: We observed significant increase in the level of LC3 (LC3-II/LC3-I) at 3 d and 7 d after SCI when compared with the sham group. While the level of beclin-1 and ratio of beclin-1/bcl-2 was found to have increased from 12 h to 24 h after injury. Cathepsin D expression was also elevated at 7 d (P<0.01).CONCLUSION: Based on the above mentioned data, we proposed that autophagy plays a role in the manifestation of cell injury following SCI.


Niu Z.,Yuncheng Central Hospital | Zhao H.,Dalian Medical University | Hou X.,Yuncheng Central Hospital
International Journal of Clinical and Experimental Pathology | Year: 2017

Literature revealed that folate metabolism imbalance may be involved in predisposition to cancer. MTHFR, MTRR and MTR are essential enzymes for maintenance of folate hemostasis and homocysteine (Hcy) in the blood. We aimed to investigate the association between homocysteine metabolism gene polymorphism (MTHFR rs1801133, MTR 1805087 and MTRR rs1801394) and risk of breast cancer in West China. A total of 466 patients with breast cancer and 470 normal control subjects were enrolled into our study. The MTHFR rs1801133, MTR 1805087 and MTRR rs1801394 polymorphism was analyzed by the polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). By logistic regression analysis, we observed that the CT (adjusted OR=2.67, 95% CI=1.60-4.45) and TT (adjusted OR=2.67, 95% CI=1.60-4.45) genotypes of MTHFR rs1801133 were significantly associated with higher risk of breast cancer, compared with the CC genotype. In addition, the CT+TT of MTHFR rs1801133 was significantly associated with risk of breast cancer in comparison to the CC genotype (adjusted OR=1.43, 95% CI=1.09-1.88). The CC genotype of MTRR rs1801394 showed higher association with an increased risk of breast cancer as compared with the AA genotype (adjusted OR=1.96, 95% CI=1.14-3.37). The results of our study indicate that the MTHFR rs1801133 and MTRR rs1801394 polymorphisms are potential risk factors for the development of breast cancer in the Chinese population.


PubMed | Shandong Provincial Hospital, Zhengzhou University, Guangdong General Hospital, PLA Fourth Military Medical University and 29 more.
Type: Journal Article | Journal: Chinese medical journal | Year: 2016

Over the years, the mechanical ventilation (MV) strategy has changed worldwide. The aim of the present study was to describe the ventilation practices, particularly lung-protective ventilation (LPV), among brain-injured patients in China.This study was a multicenter, 1-day, cross-sectional study in 47 Intensive Care Units (ICUs) across China. Mechanically ventilated patients (18 years and older) with brain injury in a participating ICU during the time of the study, including traumatic brain injury, stroke, postoperation with intracranial tumor, hypoxic-ischemic encephalopathy, intracranial infection, and idiopathic epilepsy, were enrolled. Demographic data, primary diagnoses, indications for MV, MV modes and settings, and prognoses on the 60th day were collected. Multivariable logistic analysis was used to assess factors that might affect the use of LPV.A total of 104 patients were enrolled in the present study, 87 (83.7%) of whom were identified with severe brain injury based on a Glasgow Coma Scale 8 points. Synchronized intermittent mandatory ventilation (SIMV) was the most frequent ventilator mode, accounting for 46.2% of the entire cohort. The median tidal volume was set to 8.0 ml/kg (interquartile range [IQR], 7.0-8.9 ml/kg) of the predicted body weight; 50 (48.1%) patients received LPV. The median positive end-expiratory pressure (PEEP) was set to 5 cmH2O (IQR, 5-6 cmH2O). No PEEP values were higher than 10 cmH2O. Compared with partially mandatory ventilation, supportive and spontaneous ventilation practices were associated with LPV. There were no significant differences in mortality and MV duration between patients subjected to LPV and those were not.Among brain-injured patients in China, SIMV was the most frequent ventilation mode. Nearly one-half of the brain-injured patients received LPV. Patients under supportive and spontaneous ventilation were more likely to receive LPV.ClinicalTrials.org NCT02517073 https://clinicaltrials.gov/ct2/show/NCT02517073.


PubMed | CAS Beijing Institute of Genomics, Childrens Hospital of Shanxi and Yuncheng Central Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Albinism is a diverse group of hypopigmentary disorders caused by multiple-genetic defects. The genetic diagnosis of patients affected with albinism by Sanger sequencing is often complex, expensive, and time-consuming. In this study, we performed targeted next-generation sequencing to screen for 16 genes in a patient with albinism, and identified 21 genetic variants, including 19 known single nucleotide polymorphisms, one novel missense mutation (c.1456 G>A), and one disease-causing mutation (c.478 G>C). The novel mutation was not observed in 100 controls, and was predicted to be a damaging mutation by SIFT and Polyphen. Thus, we identified a novel mutation in SLC45A2 in a Chinese family, expanding the mutational spectrum of albinism. Our results also demonstrate that targeted next-generation sequencing is an effective genetic test for albinism.


Jiang X.-W.,309th Hospital of Chinese PLA | Li X.-R.,Yuncheng Central Hospital | Zhang Y.-P.,309th Hospital of Chinese PLA
International Journal of Clinical and Experimental Medicine | Year: 2015

To investigate the changes of ribbon synapses (RS) number in cochlear hair cells in C57BL/6J mice with age. Basilar membranes within the cochlea of C57BL/6J mice aged 2, 6, 10 and 12 months were harvested (5 mice in each age group). The presynaptic and postsynaptic membranes were subject to double immunohistochemical staining and observed with a laser confocal microscope. The number of RS in each segment of basilar membrane was counted by using 3D reconstruction technique. Compared with 2-month-old mice, reduction of RS number in basilar membrane inside cochlea mainly occurred to the basal turn among C57BL/6J mice aged 6 months. The number of RS in each turn among 10-month-old mice decreased considerably, and such decrease continued in the top turn and middle turn in mice aged 12 months. In contrast, the number of RS in the basal turn increased slightly. Reduction of RS probably takes place in the early stage of C57BL/6J mice presbycusis. Early prevention of presbycusis can be achieved through measures to mitigate the reduction of RS. © 2015, E-Century Publishing Corporation. All rights reserved.


Zhang Q.,Yuncheng Central Hospital | Zhang Q.,Soochow University of China | Huang C.,Soochow University of China | Meng B.,Soochow University of China | And 3 more authors.
International Journal of Molecular Sciences | Year: 2012

Ghrelin, a 28-amino acid peptide, is mainly secreted by the stomach. Ghrelin has been shown to have neuroprotective effects. However, whether ghrelin protects the spinal cord from ischemia/reperfusion (I/R) injury is unknown. To investigate this, 60 rats were randomly divided into three different groups: the sham group (n = 20), the vehicle group (n = 20), and the Ghrelin group (100 μg/kg, n = 20). Rats were sacrificed 12, 24, 48 and 72 h after ischemia. After the evaluation of neurologic function (48 h), the spinal cords were immediately removed for the determination of myeloperoxidase (MPO) activity (12-72 h). Apoptosis was quantitatively measured using the terminal transferase UTP nick end-labeling (TUNEL) method (24 h). The expression of bax and bcl-2 were evaluated by Western blot analysis (1 h), and GHSR-1a mRNA expression was detected using reverse transcriptase polymerase chain reaction (24 h). The neurological motor function was evaluated by 'Tarlov's score'. The neurologic outcomes in the ghrelin-group were significantly better than those in the vehicle group (p < 0.05). Serum tumor necrosis factor (TNF-α) levels were assessed in the peripheral venous blood. Ghrelin decreased the serum TNF-α levels and ameliorated the down regulation of spinal cord MPO activity. The expression of ghrelin receptors (GHSR-1a) in the rat spinal cord was decreased by I/R injury and increased by ghrelin. Ghrelin reduced the TUNEL-positive rate. Greater bcl-2, HSP27, HSP70, and attenuated bax expression were observed in the ghrelin-treated rats. Our results suggest that ghrelin administration may inhibit spinal I/R injury. Moreover, the improvement of neurologic function in rats was increased after the ghrelin treatment. © 2012 by the authors; licensee MDPI, Basel, Switzerland.


Meng B.,Soochow University of China | Zhang Q.,Yuncheng Central Hospital | Huang C.,Soochow University of China | Zhang H.T.,Soochow University of China | And 2 more authors.
Journal of International Medical Research | Year: 2011

Acute spinal cord lesions result in dramatic changes in neuronal function. Studies have shown that the peroxisome proliferator-activated receptor-g agonist, rosiglitazone, has neuroprotective properties. The effect of rosiglitazone after acute spinal cord injury was examined in the present study. Rats were subjected to laminectomy only; laminectomy with spinal cord contusion injury; laminectomy with contusion injury plus 30 mg/kg body weight methylprednisolone administered 5 min after surgery; or laminectomy with contusion injury plus 2 mg/kg body weight rosiglitazone administered intra-peritoneally 5 min, 6 h and 24 h after surgery. Both drugs increased neurotrophin gene and protein expression 24 h after injury compared with injured rats without drug treatment. Rosiglitazone increased neurotrophin expression at 7 days to a greater extent than methylprednisolone. Early functional recovery was observed in rats treated with rosiglitazone. The greater increase in rosiglitazone-induced nerve growth factor expression soon after injury could explain, at least in part, the improved recovery of motor function compared with methylprednisolone or saline. ©2011 Field House Publishing LLP.


Qin Z.,Yuncheng Central Hospital | Chen H.,Soochow University of China | Bin M.,Soochow University of China | Tiansi T.,Soochow University of China | Huilin Y.,Soochow University of China
Chinese Journal of Traumatology - English Edition | Year: 2014

Objective Autophagy is involved in several neurodegenerative diseases and recently its role in acute brain injury has won increasing interest. Spinal cord injuries (SCIs) often lead to permanent neurological deficit. Therefore, in this study, we examined the profiles of autophagy-linked proteins (MAP-LC3) after SCI to investigate whether the expression of autophagy contributes to neurological deficit after SCI Methods Adult female Sprague-Dawley rats were used and randomly divided into control and SCI groups. All the rates received laminectomy at T8-T10 level. Those in the SCI group received additional exposure of the dorsal surface of the spinal cord, followed by a weight-drop injury. Thereafter we investigated the expression levels of MAP-LC3, beclin-1, Cathepsin D and the beclin-1-binding protein bcl-2 by western blot analysis at 12 h, 24 h, 3 d, 7 d, 21 d and 28 d. One-way ANOVA with Tukey post hoc test was used to compare data between groups. Results We observed significant increase in the level of LC3 (LC3-II/LC3-I) at 3 d and 7 d after SCI when compared with the sham group. While the level of beclin-1 and ratio of beclin-1/bcl-2 was found to have increased from 12 h to 24 h after injury. Cathepsin D expression was also elevated at 7 d (P < 0.01). Conclusion Based on the above mentioned data, we proposed that autophagy plays a role in the manifestation of cell injury following SCI. © 2014 Daping hospital and the Research Institute of Surgery of the Third Military Medical University.


PubMed | Lanzhou University and Yuncheng Central Hospital
Type: | Journal: Clinical neurology and neurosurgery | Year: 2016

Lumbar disc herniation (LDH) is a common disease that induces back pain and radicular pain. The most efficient method for the treatment of lumbar disc herniation is still controversial. Spontaneous regression of LDH has been recognized with the advancement of radiological diagnostic tools and can explain the reason of spontaneous relief of symptoms without treatment. The proposed hypotheses are; dehydration, retraction of the disc to the hernia in the annulus fibrosis, enzymatic catabolism and phagocytosis. In this study, the case of a patient with huge lumbar disc hernia regressing by itself has been presented and the potential mechanisms of disc regression have been discussed.

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