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Zhou S.,South China University of Technology | Zhou S.,Guangxi Yulin Normal College | Wei C.,South China University of Technology | Liao C.,South China University of Technology | Wu H.,South China University of Technology
World Journal of Microbiology and Biotechnology | Year: 2010

This study was conducted to evaluate the relationship between the function of microbes and the change in their communities during the treatment of coking wastewater using a bio-fluidized bed Anaerobic-Oxic-Oxic (A/O/O) process. Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), Gas Chromatography (GC) analysis, and the triphenyl-tetrazolium chloride (TTC) dehydrogenase activity test (TTC test) were simultaneously conducted to analyze the dynamics of microbe communities during the process of coking wastewater treatment. The study suggests that combined approaches of PCR-DGGE, GC, and TTC provided a simple and accurate method to evaluate the changes in microbial activity, microbial structure, and population size with the shifting of contaminants in different treatment processes. It demonstrates that the structure and components of a microbial community are influenced by selecting pressure during the wastewater treatment process, which in turn determines the overall functionality of the system. © 2009 Springer Science+Business Media B.V. Source


Zhou S.,South China University of Technology | Zhou S.,Guangxi Yulin Normal College | Wei C.,South China University of Technology | Ke L.,South China University of Technology | Wu H.,South China University of Technology
Journal of Microbiology and Biotechnology | Year: 2010

To verify the dominance of microorganisms in wastewater biological treatment, PCR-DGGE (denaturing gradient gel electrophoresis) was performed as a supplementary support method for screening of the dominant microorganisms from activated sludge. Results suggest that the dominant microorganisms in activated sludge are primarily responsible for strengthening its effectiveness as a biological treatment system, followed by the non-main dominant microorganisms, whereas the non-dominant microorganisms showed no effects. The degree of microbial abundance present on the profile of PCR-DGGE was in line with the treatment efficiency of augmented activated sludge with isolated cultures, suggesting that PCR-DGGE can be used as an effective supplementary method for verifying culturable dominant microorganisms in activated sludge of coking wastewater. © The Korean Society for Microbiology and Biotechnology. Source

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