Yuhuangding Hospital of Yantai
Yuhuangding Hospital of Yantai
Zhu W.,Yuhuangding Hospital of Yantai |
Liu J.,Yuhuangding Hospital of Yantai |
Zou P.,Yuhuangding Hospital of Yantai |
Chen H.,Yuhuangding Hospital of Yantai
International Journal of Clinical and Experimental Medicine | Year: 2017
The methylation of the O(6)-methylguanine DNA methyltransferase (MGMT) promoter is frequently observed in several cancer types. The potential correlation between the C>T allele at SNP rs16906252 and methylation in MGMT are being actively investigated and remain largely elusive. We studied the allelic pattern of MGMT methylation through meta-analysis. All eligible studies were identified by searching PubMed, Web of Science, Embase, and Chinese National Knowledge Infrastructure Database. Pooled odds ratio (OR) and 95% confidence interval (95% CI) were calculated using fixed- or random-effect model. Genotype and allelic frequency compared between cases and controls together with further subgroup analyses were carried out by cancer type. A total of 6 studies involving 1775 cancer cases were analyzed. Meta-analysis from this study indicated that the T allele of the rs16906252 SNP were strongly associated with increased risk for MGMT methylation in allelic model (OR = 9.70, 95% CI 4.12-22.86, Pheterogeneity = 0.021), heterozygote comparison OR = 11.28, 95% CI 4.46-28.52, Pheterogeneity = 0.024) and dominant model (OR = 6.61, 95% CI 3.23-13.54, Pheterogeneity = 0.003). Stratified analysis by cancer type indicated that the association became more prominent among glioblastoma, colorectal cancer and other cancer. Our study provides strong evidence that the T allele of a MGMT promoter-enhancer SNP is a key determinant for MGMT methylation in cancer. Clarifying this association could advance our knowledge of the influence of MGMT promoter-enhancer single nucleotide polymorphism rs16906252 on MGMT promoter methylation in cancer. © 2017, E-Century Publishing Corporation. All rights reserved.
Wang X.,Yuhuangding Hospital of Yantai |
Yu X.,Yuhuangding Hospital of Yantai |
Wang Q.,The Peoples Hospital Of Zhangqiu |
Lu Y.,Peoples Hospital Of Rizhao |
Chen H.,Yeda Hospital of Yantai
Oncology Letters | Year: 2017
This study investigated the expression of special AT-rich sequence-binding protein 1 (SATB1) and toll-like receptor 4 (TLR4) protein in breast cancer and its clinical significance. We collected breast cancer tissues from 120 patients and adjacent non-cancerous tissue from 53 patients. SATB1 was expressed in 89 cases of breast cancer (74.17%) and in 7 cases of adjacent non-cancerous tissue (13.21%). TLR4 was expressed in 70 cases of breast cancer tissues (58.33%) and in 48 cases of adjacent non-cancerous tissue (90.57%). The differences of SATB and TLR4 in breast cancer and adjacent non-cancerous tissue were statistically significant. We found a negative correlation between the expression of SATB1 and TLR4 (r=-0.624, P<0.05). The expression of SATB1 and TLR4 were not significantly correlated with age, menopause, and PR and HER-2 protein expression, but were significantly correlated with tumor size, local lymphatic metastasis, histopathological grade, tumor stage, and ER protein expression (P<0.05). Overall, SATB1 and TLR4 proteins are involved in the development of breast cancer, a finding of great significance to identify therapeutic targets and prognosis markers for breast cancer. © 2017, Spandidos Publications. All rights reserved.
Ding Y.,Qingdao University |
Pan Y.,Yuhuangding Hospital of Yantai |
Liu S.,Central Hospital of Qingdao |
Jiang F.,Peoples Hospital of Zhangqiu |
Jiao J.,Jining No1 Peoples Hospital
Cancer Biology and Therapy | Year: 2017
MicroRNAs had been proved to be pivotal regulators in nasopharyngeal carcinoma (NPC) by regulating a large amount of genes' expression. In our research, we aim to explore the functions of miR-9–3p on the metastases of NPC and figure out the potential mechanisms. First, we revealed downregulation of miR-9–3p and upregulation of fibronectin 1 (FN1), β1 integrin (ITGB1) and α5 integrin (ITGAV) expression in NPC tissues and cells compared with the normal using RNA-seq analysis, RT-qPCR, western blot and immunohistochemistry. By transfection of miR-9–3p mimics in CNE-1, CNE-2 and HONE-1 cells, we confirmed tumor-suppressing roles of miR-9–3p via suppressing EMT process by MTT, wound scratch, transwell assay and western blot. After constructing luciferase reporting plasmids and transient transfection in HEK 293T cells, we proved that FN1, ITGB1 and ITGAV were all targets of miR-9–3p. Then we manipulated the expression of miR-9–3p, FN1, ITGB1 and ITGAV in HONE-1 cells, verifying the tumor-promoting effect of FN1, ITGB1 and ITGAV on cell proliferation and metastases via facilitating EMT process of cells. Additionally, these functions of FN1, ITGB1 and ITGAV could be efficiently abrogated by overexpression of miR-9–3p. Taken together, we demonstrated that elevation of miR-9–3p suppresses the proliferation and metastases of NPC via downregulating FN1, ITGB1, ITGAV and inhibiting the EMT process, which provided a series of therapeutic targets for the treatment of NPC. © 2017 Taylor & Francis Group, LLC
PubMed | Chinese University of Hong Kong, Yuhuangding Hospital of Yantai, Shanghai JiaoTong University and Soochow University of China
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2014
Infection caused by bacteria is one of the crucial risk factors for tendon adhesion formation. Silver nanoparticles (AgNP)-loaded physical barriers were reported to be effective in anti-infection and anti-adhesion. However, high silver load may lead to kidney and liver damages. This study was designed for Ibuprofen (IBU)-loaded poly(L-lactide) (PLLA) electrospun fibrous membranes containing a low dosage of Ag to evaluate its potential in maintaining suitable anti-infection and good anti-adhesion effects. The in vitro drug release study showed a sustained release of Ag ions and IBU from the membrane. Inferior adherence and proliferation of fibroblasts were found on the Ag4%-IBU4%-loaded PLLA electrospun fibrous membranes in comparison with pure PLLA and 4% Ag-loaded PLLA membranes. In the antibacterial test, all Ag-loaded PLLA electrospun fibrous membranes prevented the adhesion of Staphylococcus aureus and Staphylococcus epidermidis. Taken together, these results demonstrate that Ibuprofen is effective in enhancing the anti-adhesion and anti-proliferation effects of 4% Ag-loaded PLLA fibrous membrane. The medical potential of infection reduction and adhesion prevention of Ag4%-IBU4%-loaded PLLA electrospun fibrous membrane deserves to be further studied.
PubMed | Nanjing Institute of Technology, Nanjing Medical University and Yuhuangding Hospital of Yantai
Type: | Journal: Scientific reports | Year: 2016
Preeclampsia is a common, pregnancy-specific disease and a major contributor to maternal and foetal morbidity and mortality. Some placental abnormalities, including deficient implantation, abnormal trophoblast cell function, and improper placental vascular development, are believed to lead to preeclampsia. The long noncoding RNA SPRY4-IT1 is more highly expressed in preeclamptic human placentas than in normal placentas. We assessed the role of epithelial-mesenchymal transition (EMT)-associated invasion and migration in HTR-8/SVneo trophoblast cells. Overexpression of SPRY4-IT1 suppressed trophoblast cell migration and invasion, whereas reduced expression of SPRY4-IT1 prevented the EMT process. Mechanistically, an RNA immunoprecipitation experiment showed that SPRY4-IT1 bound directly to HuR and mediated the -catenin expression associated with EMT in HTR-8/SVneo cells. Moreover, the expression levels of genes in the WNT family, such as WNT3 and WNT5B, were changed after transfection of HTR-8/SVneo with SPRY4-IT1. Together, our results highlight the roles of SPRY4-IT1 in causing trophoblast cell dysfunction by acting through the Wnt/-catenin pathway, and consequently in impairing spiral artery remodelling. These results suggest a new potential therapeutic target for intervention against preeclampsia.
PubMed | 1 Shandong Medical College, Shandong Cancer Hospital and Yuhuangding Hospital of Yantai
Type: Clinical Trial, Phase I | Journal: The British journal of radiology | Year: 2016
This Phase I study aimed to assess the safety and efficacy of concurrent selective lymph node (SLN) late-course accelerated hyperfractionated (LCAF) intensity-modulated radiotherapy (IMRT) and S-1 plus cisplatin (CDDP) for the locally advanced oesophageal squamous-cell carcinoma (ESCC).The total dose of SLN LCAF IMRT was 59.6Gy/34 fractions in 5.4 weeks. The concurrent chemotherapy (CCRT) was administered as follows: CDDP 25mgm(-2) on Days 1-3 and Days 22-24; S-1 was applied in a de-escalating dosage with a decrement of 10mgm(-2) per day, from its full dose level of 80mgm(-2), orally twice daily on Days 1-14 and Days 22-35. We inferred the maximum-tolerated dose (MTD), dose-limiting toxicities (DLTs) and recommended dose, according to adverse reaction during CCRT.Totally, 15 patients with ESCC with T2-4N0-1M0-1a were enrolled in Dose Level 1 (80mgm(-2)). In the initial five patients, two patients developed DLTs. As MTD was not reached, five additional patients were treated with the same dose level, and DLTs occurred in only one patient. Similar results were found in the last five patients. After CCRT, the objective response rates were 100% for primary tumours and 86.2% for metastatic lymph nodes, respectively. Totally, the observed Grade 3 toxicities during CCRT were leukopenia (20%), neutropenia (20%) and dermatitis (13.3%), and no Grade 4 toxicity occurred. The Kaplan-Meier-estimated overall and progression survival rates were 86.7% and 66.7% (1 year), 73.3% and 60% (2 years) and 73.3% and 60% (3 years).The concurrent SLN LCAF IMRT and chemotherapy with S-1 and CDDP was well tolerated and showed promising efficacy. The dose of S-1 in this regimen was recommended with 80mgm(-2) orally twice daily on Days 1-14 and Days 22-35.CCRT with S-1 plus CDDP exhibited encouraging results with milder toxicities, high objective response rates and ideal overall survival time.
Sun Y.,Yuhuangding Hospital of Yantai
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013
Research for surgical methods and experience of sinus fibrous dysplasia treatment by auxiliary nasal endoscopy image navigation. Retrospective analysis of the records of 4 patients from March 2006 to December 2011 who were diagnosed as fibrous dysplasia of nasal sinuses, 2 male and 2 female, aged from 19 yrs to 55 yrs and average age was 38 yrs; 3 of them were destroyed of sinus floor, the other who had the bone fiber of sphenoidal sinus. By using 16 multi slice spiral CT and 3D reconstruction, the 4 cases were all treated by drill grinding abnormal fibrous tissue in the nasal sinus cavities with auxiliary nasal endoscopy image navigation, to achieve contour in the bone fiber cavities. Lesions of 4 cases were accurately found and grinded under auxiliary nasal endoscopy image navigation. For 3 patients, headache, nasal congestion, facial discomfort and other symptoms have gradually disappeared after operation. However, one patient with sinus floor destroyed suffered facial swelling and pain 3 month after operation, with ineffective conservation treatment outcomes. After CT re-scan, it showed that there were a large number of bone residue in maxillary sinus during the re-operation. The reason was that the surgical cavity was not rinsed off which leaded to maxillary sinus congestion. With the methods of expansion of the maxillary sinus, flushing the cavity, and postoperative nasal and maxillary sinus rinse, symptoms disappeared 1 month later without recurrence. Patients with fibrous dysplasia of nasal sinuses are treated thoroughly, securely, and effectually, by drill grinding abnormal fibrous tissue in the nasal sinus cavities with auxiliary nasal endoscopy image navigation, to achieve contour in the bone fiber cavities.
Zhao W.,Yuhuangding Hospital of Yantai |
Bian Y.,Yuhuangding Hospital of Yantai |
Zhu W.,Yuhuangding Hospital of Yantai |
Zou P.,Yuhuangding Hospital of Yantai |
Tang G.,Yuhuangding Hospital of Yantai
Tumor Biology | Year: 2014
Regulator of telomere elongation helicase 1 (RTEL1) is critical for genome stability and tumor avoidance. Many studies have reported the associations of RTEL1 rs6010620 with glioma risk, but individually published results were inconclusive. This meta-analysis was performed to quantitatively summarize the evidence for such a relationship. The PubMed, Embase, and Web of Science were systematically searched to identify relevant studies. The odds ratio (OR) and 95 % confidence interval (95 % CI) were computed to estimate the strength of the association using a fixed or random effects model. Ten studies were eligible for meta-analysis including data on glioma with 6,490 cases and 9,288 controls. Overall, there was a significant association between RTEL1 rs6010620 polymorphism and glioma risk in all four genetic models (GG vs. AA: OR=1.87, 95 % CI=1.60-2.18, Pheterogeneity =0.552; GA vs. AA: OR=1.30, 95 % CI=1.16-1.46, Pheterogeneity =0.495; dominant model-GG+GA vs. AA: OR=1.46, 95 % CI=1.31-1.63, Pheterogeneity =0.528; recessive model-GG vs. GA+AA: OR=1.36, 95 % CI=1.27-1.46, Pheterogeneity =0.093). Subgroup analyses by ethnicity showed that RTEL1 rs6010620 polymorphism resulted in a higher risk of glioma among both Asians and Caucasians. In the stratified analysis by ethnicity and source of controls, significantly increased risk was observed for Asians and Europeans in all genetic models, population-based studies in all genetic models, and hospital-based studies in three genetic models (heterozygote comparison, homozygote comparison, and dominant model). Our meta-analysis suggested that RTEL1 rs6010620 polymorphism is likely to be associated with increased glioma risk, which lends further biological plausibility to these findings. © 2014 International Society of Oncology and BioMarkers (ISOBM).
Xiu C.,Yuhuangding Hospital of Yantai |
He Q.,Yuhuangding Hospital of Yantai |
Zou P.,Yuhuangding Hospital of Yantai |
Zhang H.,Yuhuangding Hospital of Yantai
International Journal of Clinical and Experimental Medicine | Year: 2016
X-ray cross-complementing group 4 (XRCC4) is crucial for cells to maintain genetic stability thereby inflicting carcinogenesis. To date, epidemiologic findings have reached conflicting and ambiguous conclusions on the role of XRCC4 rs1805377 polymorphism in cancer risks. We made a comprehensive quantitative evaluation by performing a meta-analysis. Eligible publications assessing the association between XRCC4 rs1805377 polymorphism and cancer risks from PubMed, Embase and China national knowledge infrastructure (CNKI) databases were indentified. We used odds ratios (ORs) with 95% confidence intervals (CIs) to assess association strengths with the fixed-effect model or the random-effects model dependent on the heterogeneity. At the same time, subgroup analysis and sensitivity analysis were conducted. A total of 8 studies including 1911 cases and 2688 controls were included based on the search criteria. It was revealed by this meta-analysis that, in the Asian population, there was significant correlation between XRCC4 rs1805377 polymorphism and the risk of cancers (GG vs. AA: OR = 1.28, 95% CI = 1.05-1.57, Pheterogeneity = 0.392). In further stratified analyses, XRCC4 rs1805377 polymorphism was associated with increased glioma risk among Asians in homozygote comparison (GG vs. AA: OR = 1.59, 95% CI = 1.12-2.25, Pheterogeneity = 0.261). Significantly elevated cancers risk were also observed in population-based studies (GG versus AA: OR = 1.38, 95% CI = 1.11-1.72, Pheterogeneity = 0.571) and using other method studies (GG versus AA: OR = 1.63, 95% CI = 1.11-2.39, Pheterogeneity = 0.275). This meta-analysis indicated that XRCC4 rs1805377 polymorphism probably was associated with gliomas susceptibility in Asians. © 2016, E-Century Publishing Corporation. All rights reserved.
Ma Z.-G.,Yuhuangding Hospital of Yantai
Journal of Leukemia and Lymphoma | Year: 2010
Objective: To study cytotoxic and antineoplastic effect in vitro of tephroseris kirilowii turez, houlub extract on U266 multiple myeloma cell line. Methods: U266 cells were cocultured with the tephroseris kirilowii turez, houlub extract. Cytotoxicity assay was used by CCK-8 detection kit. Cell cycle and apoptosis were determined using flow cytometry (FCM) analysis. Results: Extract of tephroseris kirilowii turez, houlub showed strong cytotoxicity against U266 cells.The IC 50 was about 3.2 mg/L. After exposure of U266 cells to the drug, the distribution of cell cycle was changed compared with that of the controls. When the durg concentration was 1.25, 2.5, 5 and 10 mg/L, respectively, the pencentages of cells in the G 0/G 1 phase were decreased with (34.12±0.49)%, (38.06±0.63)%, (27.46±0.61)%, (15.91±0.32)%, respectively, while those in the S phase were increased with (4.98±0.50)%, (4.01±0.22)%, (4.16±0.15)% and (5.04±0.12)% in G 2/M phase were increased with (50.05±1.12)%, (51.27±0.71)%, (51.84±0.73)% and (55.11±0.25)%, respectively, and apoptosis cells were increased. Apoptosis of U266 cells inadose-depadeut manner could be deteted with staining of Annexix V FITC/PI testing through FCM. Conclusion: Tephroseris kirilowii turez extract showed strong cytotoxic effect on U266 cells. The antineoplsastic mechanism of the drug can be partly due to its induced apoptotic effect.