PubMed | Yuebei Peoples Hospital, Guangzhou Hospital of Traditional Chinese Medicine, Shenzhen Hospital of Traditional Chinese Medicine, Guangzhou University and 5 more.
Type: | Journal: Journal of ethnopharmacology | Year: 2016
Shenmai injection (SMI) is a traditional Chinese herbal medicine extracted from Panax ginseng (Panax ginseng C.A. Mey, steamed and dry) and Ophiopogon japonicus (Ophiopogon japonicus (L.f.) Ker-Gawl, root). It has been widely used for the treatment of chronic heart failure (CHF) in China. However, the evidence supporting its effects remains unclear due to lack of high quality trials. The aim of this study was to investigate the efficacy and safety of SMI in CHF patients with coronary artery disease (CAD).This double-blind, multicenter study randomized 240 eligible patients equally to receive SMI or placebo (100ml/day) in addition to standard medicines for the treatment of CHF. The primary endpoint was the New York Heart Association (NYHA) functional classification. The secondary endpoints were 6-min walking distance (6MWD), short-form 36 (SF-36) hearth survey score, traditional Chinese medicines (TCM) syndrome score, left ventricular ejection fractions (LVEF) and B-type natriuretic peptide (BNP) level.During treatment of 1 week, the NYHA functional classification was gradually improved in both groups, but the SMI group demonstrated a significantly greater improvement compared with the placebo group (p=0.001). Moreover, the improvement in patients received SMI was superior to those in control group with respect to 6MWD, SF-36 score and TCM syndrome score. Treatment with SMI within 1 week was well tolerated with no apparent safety concerns.The integrative treatment with standard medicines plus SMI can further improve NYHA functional classification for patients with CHF and CAD. Therefore, SMI could be recommended in the combination therapy for CHF accompanied with CAD.
Li Y.,Jinan University |
Li Y.,Southern Medical University |
Liu H.,Guangdong Women and Children Hospital |
Lai C.,Jinan University |
And 3 more authors.
Cell and Tissue Research | Year: 2013
We investigate the role of the Lin28/let-7a/c-Myc pathway in non-muscle invasive bladder cancer (NMIBC). Using RT-PCR, western blot and immunohistochemistry techniques, the levels of pre-let-7a, let-7a, Lin28 and c-Myc RNA and/or proteins were determined in samples of normal bladder tissue and bladder cancer. Expression of pre-let-7a was found to be negatively correlated with the pathological grade of bladder cancer, while let-7a showed a positive correlation with bladder cancer pathological grade. Expression of Lin28 RNA and protein was not significantly different between normal bladder tissue and low-grade transitional cell carcinoma of bladder (TCC) but the expression levels in high-grade TCC were remarkably increased. Expression of c-Myc RNA and protein was significantly higher in bladder cancer samples in comparison to normal bladder tissue without correlation with cancer differentiation. Expression of all the above RNAs and proteins showed no significant difference in Ta and T1 stages. The Lin28/let-7a/c-Myc pathway plays an important role in NMIBC. In particular, expression levels of let-7a correlate with the degree of cancer differentiation but not cancer stage. © 2013 Springer-Verlag Berlin Heidelberg.
Tang H.,Sun Yat Sen University |
Wang H.,Chinese PLA General Hospital |
Bai J.,Sun Yat Sen University |
Ding M.,Yuebei Peoples Hospital |
And 6 more authors.
American Journal of Rhinology and Allergy | Year: 2012
Background: Although Interleukin (IL)-17A has been suggested to play a role in corticosteroid hyporesponsiveness, whether IL-17A is able to affect the sensitivity of MUC5AC to intranasal corticosteroid treatment in patients with allergic rhinitis (AR) is unclear. Methods: Twenty patients with moderate to severe AR were enrolled in this study and the expression of MUC5AC, IL-17A, and glucocorticoid receptor beta (GR beta) was detected using immunochemical staining and quantitative reverse transcription polymerase chain reaction (RT-PCR) before and after treatment with fluticasone propionate (FP) nasal spray for 4 weeks, respectively. In addition, the effects of FP on IL-13- and IL-17A-induced MUC5AC and GR beta were also evaluated in the primarily cultured human nasal epithelial cells (HNECs) in vitro. Results: The increased MUC5AC expression was associated with IL-17A levels in AR, and IL-17A was found to affect the inhibition of MUC5AC by corticosteroid treatment. Both IL-13 and IL-17A significantly promoted MUC5AC mRNA expression in HNECs, and FP treatment was able to significantly inhibit MUC5AC mRNA expression in HNECs induced by IL-13 but not for that induced by IL-17A. Also, IL-17A but not IL-13 promoted GR beta mRNA expression in HNECs, which was not affected by administration of corticosteroid. Conclusion: Our results suggest that the sensitivity of MUC5AC to topical corticosteroid is negatively associated with IL-17A in AR patients. This might help us to gain more insight into the pathophysiology and the pharmacotherapeutic mechanisms on AR treatment. Copyright © 2012, OceanSide Publications, Inc.
PubMed | U.S. Center for Disease Control and Prevention, Yuebei Peoples Hospital, Sun Yat Sen University and Shijie Hospital of Dongguan
Type: Journal Article | Journal: International journal of environmental research and public health | Year: 2016
Epidemics of hand, foot and mouth disease (HFMD) among children have caused concern in China since 2007. We have conducted a retrospective study to investigate risk factors associated with HFMD. In this non-matching case-control study, 99 HFMD patients and 126 control from Guangdong Province were enlisted as participants. Data comprising demographic, socio-economic, clinical and behavior factors were collected from childrens parents through face-to-face interviews by trained interviewers using a standardized questionnaire. Results of the primary logistic regression analyses revealed that age, history of cold food consumption, hand-washing routines, and airing out bedding were significantly associated with HFMD cases. Results of further multivariate analysis indicated that older age (OR = 0.44, 95% CI: 0.34-0.56) and hand-washing before meals (OR = 0.3, 95% CI: 0.13-0.70) are protective factors, whereas airing out bedding more than thrice a month (OR = 4.55, 95% CI: 1.19-17.37) was associated with increased risk for HFMD. Therefore, hand-washing should be recommended to prevent HFMD, and the potential threat of airing out bedding should be carefully considered. However, further studies are needed to examine other possible risk factors.
PubMed | U.S. Center for Disease Control and Prevention, Yuebei Peoples Hospital and Sun Yat Sen University
Type: | Journal: Scientific reports | Year: 2017
The objective of this study was to identify potential risk factors for severe hand, foot and mouth disease (HFMD). In this case-control study, 459 severe HFMD patients and 246 mild HFMD patients from Guangdong province and Henan province, China were included. Data comprising demographic characteristics, clinical symptoms and signs, laboratory findings and other factors were collected. Univariate analysis revealed 30 factors associated with severe cases. Further multivariate analysis indicated four independent risk factors: fatigue (p<0.01, odd ratio [OR]=204.7), the use of glucocorticoids (p=0.03, OR=10.44), the use of dehydrant drugs (p<0.01, OR=73.7) and maculopapular rash (p<0.01, OR=84.4); and one independent protective factor: herpes or ulcers in mouth (p=0.01, OR=0.02). However, more systematic research and validation are needed to understand the underlying risk factors for severe HFMD.
Weng W.,Tongji University |
Yang Q.,Tongji University |
Huang M.,Yuebei Peoples Hospital |
Qiao Y.,Tongji University |
And 5 more authors.
Biochemical and Biophysical Research Communications | Year: 2011
Tumor protein p53-induced nuclear protein 1 (TP53INP1) is a well known stress-induced protein that plays a role in both cell cycle arrest and p53-mediated apoptosis. Loss of TP53INP1 expression has been reported in human melanoma, breast carcinoma, and gastric cancer. However, TP53INP1 expression and its regulatory mechanism in esophageal squamous cell carcinoma (ESCC) remain unclear. Our findings are in agreement with previous reports in that the expression of TP53INP1 was downregulated in 28% (10/36 cases) of ESCC lesions, and this was accompanied by significant promoter methylation. Overexpression of TP53INP1 induced G1 cell cycle arrest and increased apoptosis in ESCC cell lines (EC-1, EC-109, EC-9706). Furthermore, our study showed that the oncoprotein c-Myc bound to the core promoter of TP53INP1 and recruited DNA methyltransferase 3A to methylate the local promoter region, leading to the inhibition of TP53INP1 expression. Our findings revealed that TP53INP1 is a tumor suppressor in ESCC and that c-Myc-mediated DNA methylation-associated silencing of TP53INP1 contributed to the pathogenesis of human ESCC. © 2011 Elsevier Inc.
PubMed | Yuebei Peoples Hospital and Southern Medical University
Type: Journal Article | Journal: Oncology letters | Year: 2016
Despite extensive investigations of therapeutic improvements for surgical techniques, chemotherapy and chemoradiotherapy, esophageal squamous cell carcinoma (ESCC) remains one of the most aggressive forms of cancer, and the prognosis for patients with advanced ESCC remains poor. Therefore, effective therapies are urgently required in order to improve the prognosis of patients with ESCC. TE-1 cells were treated with gambogic acid (GA), and then subjected to western blot analysis, TUNEL assay and caspase activity analysis. GA significantly induced apoptosis in ESCC TE-1 cells. In addition, the antitumor activity of GA was accompanied by the decreased expression of phosphorylated-protein kinase B (p-AKT) and nuclear factor of light polypeptide gene enhancer in B-cells 1 (NF-B). The inhibition of protein kinase B (AKT) and NF-B activation by chemical inhibitors augmented the apoptotic effect responses to GA in the TE-1 cells. The pan-caspase inhibitor z-VAD-fmk (zVAD) decreased GA-induced apoptosis. Furthermore, zVAD attenuated GA-induced growth inhibition in TE-1 cells. GA induced apoptosis in ESCC TE-1 via suppression of NF-B pathway. The findings of the present study may provide a novel insight into ESCC treatment.
Liu S.-P.,Yuebei Peoples Hospital |
Liu G.-Q.,Yuebei Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Once traumatic arthritis appears, degeneration of the joints will be inevitable. In the clinical treatment of moderate or less stiff elbow, traditional elbow stiffness release is the preferred repair method. However, its therapeutic effect is controversial in the clinical treatment of very severe elbow stiffness. The focus on conflict is articular surface damage after the implementation of the traditional release of elbow stiffness. OBJECTIVE: To observe the repair effect of open release combined with hinged external fixator on extremely severe elbow stiffness. METHODS: A total of 52 patients with extremely severe elbow joint stiffness were selected from the Third Department of Orthopedics, Yuebei People’s Hospital from May 2012 to July 2014. All patients signed the informed consent. In accordance with the approval of Hospital Ethics Committee, they were divided into control and study groups (n=26). Control group received the traditional elbow stiffness release. Study group received open release combined with hinged external fixator. The changes in flexion-extension range-of-motion and Mayo elbow joint function score were compared between the two groups. RESULTS AND CONCLUSION: After treatment with open release combined with hinged external fixator, significant differences in excellent and good rate, range-of-motion of flexion-extension and Mayo elbow joint function score were detected between the study group [96%, (117.5±20.5)°, (88.5±11.5) points] and the control group [77%, (93.5±18.5)°, (76.5±9.5) points] (P < 0.05). These results indicated that open release combined with hinged external fixator significantly improved elbow joint function in patients with extremely severe elbow stiffness, facilitated postoperative rehabilitation exercise activities, and had high value of clinical application. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Xie D.,Yuebei Peoples Hospital |
Zhu L.,Yuebei Peoples Hospital |
Liu H.,Yuebei Peoples Hospital |
Zeng C.,Yuebei Peoples Hospital
Journal of Acupuncture and Tuina Science | Year: 2012
Objective: To observe the event related potential 300 (P300) after scalp acupuncture treatment in patients with cognitive disorder due to cerebral infarction, and evaluate its therapeutic effect. Methods: Sixty-six patients aged below 70 years old with cognitive disorder after initial onset of cerebral infarction were randomized into a treatment group and a control group. Conventional neurological drugs and physical trainings were prescribed to both groups. In addition, the treatment group received scalp acupuncture. The two groups were compared by evaluating the latency and amplitude of P300 before and after the 3-month treatment. Results: After treatment, the latency of P300 was shortened by 39.1 ms in the treatment group, versus 16.7 ms in the control group, and the difference was statistically significant (P<0.05). Conclusion: P300 can objectively evaluate the therapeutic effect of scalp acupuncture in treating the patients with cognitive disorder due to cerebral infarction. Scalp acupuncture has positive effect in treating this group of patients. © 2012 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.
Liu Z.,Harbin Medical University |
Guo Y.,Yuebei Peoples Hospital |
Li J.,Harbin Medical University |
Xu J.,Harbin Medical University |
Liu B.,Harbin Medical University
Digestive Diseases and Sciences | Year: 2013
Introduction: Colorectal cancer is one of the common malignant tumors in humans, and the incidence rate is gradually increasing year by year. Survivin and CD44v3 are ideal targets for gene therapy due to their overexpression in colorectal cells. Studies show that downregulation of survivin could promote apoptosis and depress proliferation, and reduction of CD44v3 expression could inhibit tumor invasive capacity. It is difficult to achieve satisfactory curative effect. Objective: In this study, we use survivin and CD44v3 short hairpin RNAs (shRNA) combined transfection into colorectal cancer cell line SW480 to investigate its effects on the cell apoptosis, proliferation and invasiveness. Methods: ShRNA plasmids targeting survivin and CD44v3 were singly or co-transfected into SW480 cells. Results: The co-transfection group exhibited the most significant inhibitory effect on cell growth (P < 0.05) and the highest apoptosis rate (P < 0.05). In addition, the invasive capacity in the co-transfected group was the least. The tumor inhibition rate of the cotransfected group in xenograft tumor mice was significantly higher than other groups (P < 0.05). Moreover, the microvessel density of the co-transfected group was significantly decreased compared with other groups (P < 0.05). Conclusion: These results suggest combined transfection of survivin shRNA and CD44v3 shRNA may produce a synergistic effect on gene therapy in colorectal cancer. © 2013 Springer Science+Business Media New York.