Xie X.,Sun Yat Sen University |
Xie X.,Hainan University |
Peng J.,Yue Bei Peoples Hospital |
Chang X.,Sun Yat Sen University |
And 6 more authors.
Molecular and Cellular Endocrinology | Year: 2013
Both RhoA/ROCK and NF-κB signaling pathways play important roles in the pathogenesis of diabetic nephropathy (DN). However, it remains unknown whether and how RhoA/ROCK regulates NF-κB signaling in diabetic kidneys. In cultured glomerular mesangial cells (GMCs), the high glucose-activated NF-κB nuclear translocation and DNA binding activity were attenuated by ROCK inhibitor Y27632 or dominant-negative RhoA mutant, indicating that RhoA/ROCK signaling regulates high glucose-activated NF-κB pathway. Furthermore, NF-κB-regulated inflammatory factors ICAM-1 and TGF-β1 were markedly increased in high glucose-treated GMCs, leading to accumulation of fibronectin (FN), an important component of extracellular matrix (ECM), This effect was also effectively attenuated by Y27632 or dominant-negative RhoA mutant. In STZ-induced diabetic rats, treatment with ROCK inhibitor fasudil suppressed the RhoA/ROCK activation and NF-κB nuclear translocation, and significantly reduced the renal FN, ICAM-1 and TGF-β1 protein levels. Thus, the RhoA/ROCK pathway may regulate NF-κB to upregulate inflammatory genes and mediate the development of DN. © 2013 Elsevier Ireland Ltd.
Zhao Y.,Guangdong Provincial Hospital of Chinese Medicine |
Zhao M.,Southern Medical University |
Li X.,JiangmenWuyi Traditional Chinese Medicine Hospital |
Ma X.,Yue Bei Peoples Hospital |
And 4 more authors.
PLoS ONE | Year: 2014
Objective: Evaluate the predictive value of Boston Acute Stroke Imaging Scale (BASIS) in acute ischemic stroke in Chinese population.Methods: This was a retrospective study. 566 patients of acute ischemic stroke were classified as having a major stroke or minor stroke based on BASIS. We compared short-term outcome (death, occurrence of complications, admission to intensive care unit [ICU] or neurological intensive care unit [NICU]), long-term outcome (death, recurrence of stroke, myocardial infarction, modified Rankin scale) and economic index including in-hospital cost and length of hospitalization. Continuous variables were compared by using the Student t test or Kruskal-Wallis test. Categorical variables were tested with the Chisquare test. Cox regression analysis was applied to identify whether BASIS was the independent predictive variable of death.Results: During hospitalization, 9 patients (4.6%) died in major stroke group while no patients died in minor stroke group (p,0.001), 12 patients in the major stroke group and 5 patients in minor stroke group were admitted to ICU/NICU (p<0.001). There were more complications (cerebral hernia, pneumonia, urinary tract infection) in major stroke group than minor stroke group (p,0.05). Meanwhile, the average cost of hospitalization in major stroke group was 3,100 US$ and 1,740 US$ in minor stroke group (p<0.001); the average length of stay in major and minor stroke group was 21.3 days and 17.3 days respectively (p<0.001). Results of the followup showed that 52 patients (26.7%) died in major stroke group while 56 patients (15.1%) died in minor stroke group (P<0.001). 62.2% of the patients in major stroke group and 80.4% of the patients in minor stroke group were able to live independently (P<0.002). The survival analysis showed that patients with major stroke had 80% higher of risk of death than patients with minor stroke even after adjusting traditional atherosclerotic factors and NIHSS at baseline (HR51.8, 95% CI: 1.1-3.1).Conclusion: BASIS can predict in-hospital mortality, occurrence of complication, length of stay and hospitalization cost of the acute ischemic stroke patients and can also estimate the long term outcome (death and the dependency). BASIS could and should be used as a dichotomous stroke classification system in the daily practice. © 2014 Zhao et al.
Li J.,Yue Bei Peoples Hospital
Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation | Year: 2011
The work presented here was to research into a kind of automatic control system that would be automatically linked to the working status of electronic endoscope. The image video output signal and signal of negative pressure sensor were used to be a sampling signal of automatic control circuit. An electronic switch of automatic control circuit was challenged the manual control device of the negative pressure system. This system simplifies the operation procedures, enhances work efficiency, reduces energy consumption and improves the service life of the machine. The paper concludes that this negative pressure automatic control system has many strengths like stable performance, easy installation and strong versatility. It also can be widely applied in different brand and different types of electronic endoscope in China.
Xie X.,Sun Yat Sen University |
Peng J.,Yue Bei peoples Hospital |
Huang K.,Sun Yat Sen University |
Huang J.,Sun Yat Sen University |
And 3 more authors.
Molecular and Cellular Endocrinology | Year: 2012
A number of studies have recently demonstrated the involvement of nuclear factor-kappa B (NF-κB) activation and the subsequent coordinated inflammatory responses in the pathogenesis of diabetic nephropathy (DN). Polydatin has been shown to have the ability of anti-adhesive inflammation. However, the possible protective and beneficial effects of polydatin on DN via suppressing inflammatory damage and extracellular matrix (ECM) accumulation are not fully elucidated. We found that the polydatin could inhibit the induction and activity of NF-κB, and meanwhile ameliorating ECM accumulation in streptozotocin-diabetic rats. We aimed to investigate the effect of polydatin on fibronectin (FN) protein expression, and to elucidate its potential mechanism involving the NF-κB inflammatory signaling pathway in rat glomerular mesangial cells (GMCs) cultured under high glucose. The results revealed that polydatin significantly suppressed high glucose-induced FN production, inhibited NF-κB nuclear translocation, reduced the DNA-binding activity of NF-κB, as well as decreased the protein expression of ICAM-1 and TGF-β in GMCs. These findings suggested that polydatin significantly represses high glucose-induced FN expression in rat GMCs, which may be closely related to its inhibition of the NF-κB signaling pathway. Hence, we elucidated the potential mechanisms of the anti-inflammatory effects and ECM accumulation alleviation of polydatin in GMCs of DN in vitro. © 2012 Elsevier Ireland Ltd.
Lin X.,Southern Medical University |
Xu W.,Southern Medical University |
Shao M.,Southern Medical University |
Fan Q.,Southern Medical University |
And 4 more authors.
BMC Complementary and Alternative Medicine | Year: 2015
Background: Shenling Baizhu San (SBS) is a well-known and classical Chinese medicine formula. It has been used for treatment of gastrointestinal disorders for about nine hundred years. Recent reports showed that it was effective in curing colitis and ameliorating the major manifestations of postoperational colorectal cancer (CRC). This study was to evaluate the effects of SBS on azoxymethane (AOM) and dextran sodium sulfate (DSS) induced colitis associated CRC (caCRC) and to analyze the underlying mechanism of SBS in preventing CRC. Methods: The colon tissue of mice in different group was determined by immunohistochemistry and western blot. TGF-β1 in serum was measured by ELISA. Myeloid-derived suppressor cells (MDSCs) were identified by flow cytometry and immunohistochemistry. Results: The formed neoplasms phenotypically resembled human caCRC with upregulated β-catenin, p53 and proliferating cell nuclear antigen (PCNA). SBS treatment reduced the death rate of mice and decreased the incidence and multiplicity of colonic neoplasms. SBS decreased the number of MDSCs and the level of transforming growth factor β1 (TGF-β1). SBS alleviated epithelial mesenchymal transition (EMT) through downregulating N-cadherin (N-cad), Vimentin, Fibronectin, Snail, and upregulating E-cadherin (E-cad). It reduced the activation of Wnt5a and EMT induced by TGF-β1. Conclusions: SBS reduced the death rate through decreasing the incidence and multiplicity of colonic tumors. SBS lowered MDSCs infiltration and inhibited TGF-β1 induced EMT to exert its anti-caCRC effects. © Lin et al.; licensee BioMed Central.
Chen B.-F.,Yue Bei Peoples Hospital |
Xu X.,Yue Bei Peoples Hospital |
Deng Y.,Yue Bei Peoples Hospital |
Ma S.-C.,Yue Bei Peoples Hospital |
And 3 more authors.
Helicobacter | Year: 2013
Background: Helicobacter pylori (H. pylori) infection stimulates the production of proinflammatory cytokines associated with the development of atherosclerosis. Levels of circulating interleukin-18 (IL-18) have been positively correlated with carotid intima-media thickness (IMT) and coronary plaque area and have identified IL-18 levels as important predictors of coronary events and cardiovascular mortality. This study aimed to examine the relationship between serum IL-18 and H. pylori-IgG antibody as a sign of H. pylori infection in patients with carotid atherosclerosis. Methods: The carotid IMT, traditional atherosclerotic risk factors, levels of serum H. pylori-IgG and IL-18 were measured in 573 health checkup examinees. Results: Serum IL-18 and H. pylori-IgG levels were significantly increased in subjects with increased IMT in comparison with those with normal IMT. In subjects with increased IMT, serum H. pylori-IgG was positively correlated with serum IL-18 (r = .402, p = .002), and the association was independent of traditional atherosclerotic risk factors (β = 0.310, p < .001). Conclusions: In health checkup examinees with increased IMT, serum IL-18 and H. pylori-IgG were independently correlated and were significantly higher than in subjects with normal IMT. © 2012 Blackwell Publishing Ltd.
Huang Y.-X.,Guangzhou University |
Huang Y.-X.,Guangzhou Nansha Central Hospital |
Jia L.,Guangzhou University |
Jia L.,Guangzhou Nansha Central Hospital |
And 5 more authors.
Pancreas | Year: 2014
OBJECTIVE: This study aimed to investigate the clinical features and incidence trends of hyperlipidemic acute pancreatitis (HLAP) of multicenter studies in Guangdong, China, for 15 years. METHODS: The medical records of 1582 patients with acute pancreatitis who were admitted to 4 general hospitals of Guangdong from January 1990 to December 2005 were reviewed. The inpatient medical and radiologic records were reviewed to determine clinical features, severity, complications, mortality, and recurrence rate. RESULTS: A total of 7.8% (123/1582) patients met the HLAP criteria. Incidence of HLAP was approximately 2.6 times increased during 15 years (3.4% in 1990-1994, 5.9% in 1995-1999, and 8.9% in 2000-2005, respectively) and ranged from 3.3% to 15.5% in 4 hospitals across Guangdong. A history of diabetes was present in 31.7% and alcohol use in 18.7%. The mean (SD) triglyceride levels were 13.6 (7.2) mmol/L. Amylase was elevated higher than normal in 81.2% but only 2 times normal in 17.1% and 3 times normal in 37.6%. The frequency of severe acute pancreatitis, organ dysfunction, rate of recurrence, and mortality of HLAP was significantly higher than biliary-induced pancreatitis. CONCLUSIONS: The incidence of HLAP had significantly increased during the past 15 years with a clear geographic variation and remarkable severity and recurrent trend. Copyright © 2014 Lippincott Williams & Wilkins.
PubMed | Chongqing Medical University and Yue Bei Peoples Hospital
Type: | Journal: Annals of transplantation | Year: 2015
This study aimed to investigate the efficacy of hepatitis B surface antigen (HBsAg)-pulsed dendritic cell (DC) complex in the induction and maintenance of anti-HBs in immunosuppressed rats after liver transplantation.Lewis-Brown Norway (BN) rat liver transplantation models were successfully established. Recipients were injected with tacrolimus (2 mg/Kg) daily post-operation for three months to maintain immunosuppression state; the recipients were then randomly divided into two groups: HBsAg-DC group (n=15) comprised rats intraperitoneally injected with HBsAg-DC complex at 14 and 28 d post-surgery and HBsAg group (n=15) comprised rats injected with HBsAg (200 L) once a week for 12 weeks. Untreated rats post-transplantation were included in the control group (n=5). Histopathological changes were detected by light microscopy and transmission electron microscopy; mRNA expressions of IL-2 and IFN- in graft liver were analyzed through real-time polymerase chain reaction. Serum IL-2 and IFN- levels and anti-HB titer were detected through enzyme-linked immunosorbent assay. Changes in CD4+ and CD8+ T cells in the blood were detected through flow cytometry.IL-2 and IFN- expressions were lower in HBsAg-DC and HBsAg groups than in the control group (P<0.05). A high FK506 dose also induced a milder allograft rejection than the control dose. These findings showed that a high FK506 dose caused immunosuppression in rats after liver transplantation. A high anti-HB titer was detected in the HBsAg-DC group in one, two, and three months post-operation; by contrast, anti-HB titer was barely detected in the HBsAg group.High anti-HB titers could be induced and maintained in immunosuppressed rats; therefore, HBsAg-DC complex may prevent HBV reinfection after recipients undergo liver transplantation.
PubMed | Sun Yat Sen University and Yue Bei Peoples Hospital
Type: Journal Article | Journal: Clinical pharmacokinetics | Year: 2016
5-Fluorouracil plus cisplatin is the most commonly used chemotherapy regimen for nasopharyngeal carcinoma (NPC). The objective of this study was to establish an individualized 5-fluorouracil treatment model based on pharmacokinetic and pharmacodynamic analyses of 5-fluorouracil in East-Asian NPC patients.A total of 122 NPC patients were administered 5-fluorouracil plus cisplatin treatment. Blood samples were collected to calculate the area under the concentration-time curve (AUC) for 5-fluorouracil, and expressions of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) at both protein and messenger RNA (mRNA) levels were analyzed in the tumor tissues from 73 patients in the same cohort.The results showed a wide (sevenfold) pharmacokinetic variability of 5-fluorouracil exposure (measured as AUC) based on body surface area (BSA) dosing. Pharmacokinetic analyses revealed that the 5-fluorouracil AUC range had a significant impact on the response of patients to 5-fluorouracil and related toxicities. Patients with 5-fluorouracil AUC<25mgh/L responded unsatisfactorily to 5-fluorouracil (overall response rate [ORR] 17.5% lower than patients with AUC 25-35, p=0.176; and 26.1% lower than patients with AUC>35, p=0.031). On the other hand, patients with 5-fluorouracil AUC >35mgh/L experienced more 5-fluorouracil-related toxicities (a grade 3 or higher toxicity rate 57.1% higher than patients with AUC25-35, p<0.001; and 60.0% higher than AUC >35, p<0.001). The established 5-fluorouracil therapeutic window in head and neck cancer (HNC) [AUC 25-35mgh/L) was verified in our study. Pharmacodynamic analyses indicated a positive correlation between TS and DPD expression (p<0.001) and, despite the pharmacokinetic influences, low expression of TS mRNA in tumor tended to have a better ORR (81.0 vs. 54.3%, p=0.051). No significant association was found between DPD expression and ORR.The therapeutic window of 5-fluorouracil for East-Asian NPC patients was verified as 25-35mgh/L based on lower toxicity and higher efficacy. TS mRNA expression showed potentially predictive value in 5-fluorouracil treatment, and personalized treatment based on pharmacokinetics and pharmacodynamics proved to be clinically beneficial and is worthy of further clinical studies.
PubMed | Guangzhou University, Shaoguan University, Sun Yat Sen University and Yue Bei Peoples Hospital
Type: Journal Article | Journal: Neural regeneration research | Year: 2016
NgR, the receptor for the neurite outgrowth inhibitor Nogo-66, plays a critical role in the plasticity and regeneration of the nervous system after injury such as ischemic stroke. In the present study, we used immunohistochemistry to investigate the regional expression of NgR in rat brain following middle cerebral artery occlusion (MCAO). NgR protein expression was not observed in the center of the lesion, but was elevated in the marginal zone compared with control and sham-operated rats. The cerebral cortex and hippocampus (CA1, CA2, and CA3) showed the greatest expression of NgR. Furthermore, NgR expression was higher in the ipsilesional hemisphere than on the control side in the same coronal section. Although time-dependent changes in NgR expression across brain regions had their own characteristics, the overall trend complied with the following rules: NgR expression changes with time showed two peaks and one trough; the first peak in expression appeared between 1 and 3 days after MCAO; expression declined at 5 days; and the second peak occurred at 28 days.