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Guo C.,Guangxi Medical University | Li R.,Guilin Medical University | Zheng N.,Guangxi Medical University | Xu L.,Youjiang Medical University for Nationalities | And 2 more authors.
International Immunopharmacology

Diabetes mellitus is a clinically complex disease characterized by the dysfunctions of pancreas. In this study, we investigated the therapeutic effects of ramulus mori polysaccharides (RMP) on diabetic mice induced by streptozotocin. Our results showed that body weight and insulin level were notably increased after metformin and RMP treatments, while the blood glucose was lowered. HE-staining assay showed that the treatments mitigated the pathological lesions in pancreas tissue. In addition, the expression levels of tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), interleukin-6 (IL-6) and cyclo-oxygenase-2 (COX-2) were effectively reduced in pancreas tissue by the treatments, respectively. We also found that upon these treatments, the activities of manganese superoxide dismutase (MnSOD) and glutathione reductase (GSH-Rd) were increased; the content of malonaldehyde (MDA) was decreased in pancreas tissue; and the mRNA expression of heme oxygenase-1 (HO-1) was markedly increased in pancreas tissue. Taken together, these results suggest that RMP plays the blood glucose-lowering and metabolism-normalizing roles, and it may improve the function of pancreas through inhibiting the inflammatory response and attenuating the oxidative stress in pancreas tissue. © 2013 Elsevier B.V. All rights reserved. Source

Li R.,Guilin Medical University | Liang T.,Guangxi Medical University | Xu L.,Youjiang Medical University for Nationalities | Zheng N.,Guangxi Medical University | And 2 more authors.
Brain Research

An increasing number of studies suggest that oxidative stress is associated with the Parkinsonian process. This study evaluated the potential neuroprotective role of puerarin (PR) on lesioned substantia nigra (SN) induced by 6-hydroxydopamine (6-OHDA). Data from a rotational test showed that PR treatment significantly decreased apomorphine-induced rotations. Both the dopamine (DA) content in the SN and the endogenous expression of brain-derived neurotrophic factor (BDNF) were also elevated by the treatment. Pathological examination showed that dopaminergic neuronal degeneration in the SN was attenuated by PR treatment. Meanwhile, the contents of γ-glutamylcysteine synthetase (γ-GCS), glutathione (GSH) and catalase (CAT) in SN tissue were gradually elevated. Additionally, cytochrome c oxidase (COX) mRNA expression in the SN was markedly up-regulated. At the same time, nuclear factor E2-related factor 2 (Nrf2) and Kelch-like ECH-associated protein 1 (Keapl) levels were progressively increased by the PR treatment. Our findings indicated that puerarin effectively protects against 6-OHDA-mediated oxidative stress injury in SN neurons, in which the underlying mechanisms are involved in modulating BDNF expression and activating the Nrf2/ARE signaling pathway. © 2013 Elsevier B.V. Source

Guo C.,Guangxi Medical University | Xu L.,Youjiang Medical University for Nationalities | He Q.,Guangxi Medical University | Liang T.,Guangxi Medical University | And 2 more authors.
Food and Chemical Toxicology

Hepatic fibrosis (HF) is a chronic disease, which primarily leads to liver unregulated metabolism. In this study, we aimed to investigate the therapeutic effects of puerarin (PR), an active ingredient from kudzu root, on CCl4-induced HF rats. PR effectively ameliorated the liver metabolic function, resulting in reduced serum enzymatic activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total-bilirubin (T-bilirubin), extracellular matrix (ECM) contents and increased levels of albumin, total-protein (T-protein) in HF rats. Similarly, pathological examination showed that the CCl4-lesioned liver was mitigated by PR treatments. Meanwhile, we also detected significantly reduced levels of hydroxyproline (Hyp), type III precollagen (PCIII) and collagen I (Col I) in the liver tissue of HF rats, whereas the peroxisome proliferator-activated receptor-gamma (PPAR-γ) expression was effectively increased. Moreover, the expression of tissue inhibitor of metal protease-1 (TIMP-1) was decreased, while the expression of matrix metalloproteinase-2 (MMP-2) was increased. In addition, the expression of p-PI3K and p-Akt was significantly down-regulated by PR treatments. Taken together, PR could attenuate the CCl4-induced toxicity in the hepatocytes of HF rats. It played a protective role in the liver tissue probably through regulating the PPAR-γ expression and blocking the PI3K/Akt pathway to inhibit the excessive deposition of collagen. © 2013. Source

Li R.,Guilin Medical University | Xu L.,Youjiang Medical University for Nationalities | Liang T.,Guangxi Medical University | Li Y.,Guilin Medical University | And 2 more authors.
Food and Chemical Toxicology

This study was designed to evaluate the potential effects of puerarin (PR), an effective isoflavonoid compound purified from Pueraria lobata, in treating hepatic fibrosis (HF) rats induced by carbon tetrachloride (CCl4, 2mLkg-1d-1). Compared to model control, PR treatment effectively lowered the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (Alb), total protein (TP) in HF rats. Masson stained analysis showed that the condition of HF rats was mitigated. Meanwhile, the tumor necrosis factor alpha (TNF-α), nuclear factor-kappa B (NF-κB) expressions were significantly down-regulated at protein level by PR intervention. Additionally, the activity of superoxide dismutase (SOD) was elevated, while the content of malondialdehyde (MDA) was lessened in liver tissue. As revealed by immunohistochemistry assay, PR therapy resulted in reduced production of transforming growth factor-βl (TGF-βl). Moreover, it also was attributed to decreased mRNA level of inducible nitric oxide synthase (iNOS) using RT-PCR analysis. These findings demonstrate that puerarin successfully reverses hepatotoxicity in CCl4-induced HF rats via the underlying mechanisms of regulating serum enzymes and attenuating TNF-α/NF-κB pathway for anti-inflammation response, as well as improving metabolic function in liver tissue. © 2012 Elsevier Ltd. Source

Li R.,Guilin Medical University | Liang T.,Guangxi Medical University | Xu L.,Youjiang Medical University for Nationalities | Li Y.,Guilin Medical University | And 2 more authors.
Food and Chemical Toxicology

This study was designed to investigate the potential effects of 14days' intragastrically given of cinnamon polyphenols (CPS) in treating diabetic mice induced by intraperitoneal injection of streptozotocin (150mgkg-1) and fed high-sugar, high-fat diet. The diabetic mice model was successfully established through determining on fasting blood-glucose (FBG) test. As revealed by glucose oxidase (GOD) and radioimmunoassay (RIA), both dimethyldiguanide (DC, 0.6gkg-1d-1) and CPS (0.3, 0.6, 1.2gkg-1d-1) treatments significantly resulted in down-regulation of blood glucose and insulin levels in serum, while the levels of oxidative stress markers were markedly lowered through ELISA assay. Meanwhile, the pathological damage in islet with pancreatic beta cells was ameliorated by treatment of CPS at different doses, as shown in HE stain. At the same time, the treatments also caused notable reduction of iNOS, NF-κB expressions showing in Western blot analysis. These findings demonstrate that cinnamon polyphenols can exert the hypoglycemic and hypolipidemic effects through the mechanisms that may be associated with repairing pancreatic beta cells in diabetic mice and improving its anti-oxidative capacity, as well as attenuating cytotoxicity via inhibition of iNOS, NF-κB activation. © 2012. Source

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