Naka, Japan
Naka, Japan

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Iwamoto T.,Kobe Institute of Health | Arikawa K.,Kobe Institute of Health | Nakajima C.,Hokkaido University | Nakanishi N.,Kobe Institute of Health | And 9 more authors.
Infection, Genetics and Evolution | Year: 2014

The PE (Pro-Glu) and PPE (Pro-Pro-Glu) multigene families are unique to mycobacteria, and are highly expanded in the pathogenic members of this genus. We determined the intra-subspecies genetic variability of the MACPPE12 gene, which is a specific PPE gene in Mycobacterium avium subsp. hominissuis (MAH), using 334 MAH isolates obtained from different isolation sources (222 human isolates, 145 Japanese and 77 Korean; 37 bathroom isolates; and 75 pig isolates). In total, 31 single-nucleotide polymorphisms (SNPs), which consisted of 16 synonymous SNPs and 15 nonsynonymous SNPs, were determined through comparison with the MACPPE12 gene sequence of MAH strain 104 as a reference. As the result, the 334 MAH isolates were classified into 19 and 13 different sequevars at the nucleic acid level (NA types) and amino acid level (AA types), respectively. Among the 13 AA types, only one type, the AA02 type, presented various NA types (7 different types) with synonymous SNPs, whereas all other AA types had a one-to-one correspondence with the NA types. This finding suggests that AA02 is a longer discernible lineage than the other AA types. Therefore, AA02 was classified as an ancestral type of the MACPPE12 gene, whereas the other AA types were classified as modern types. The ubiquitous presence of AA02 in all of the isolation sources and all different sequevars classified by the hsp65 genotype further supports this classification. In contrast to the ancestral type, the modern types showed remarkable differences in distribution between human isolates and pig isolates, and between Japanese isolates and Korean isolates. Divergence of the MACPPE12 gene may thus be a good indicator to characterize MAH strains in certain areas and/or hosts. © 2013 Elsevier B.V.


PubMed | Rakuno Gakuen University, Kobe Institute of Health, Osaka City University, Yoshijima Hospital and 5 more.
Type: | Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases | Year: 2014

The PE (Pro-Glu) and PPE (Pro-Pro-Glu) multigene families are unique to mycobacteria, and are highly expanded in the pathogenic members of this genus. We determined the intra-subspecies genetic variability of the MACPPE12 gene, which is a specific PPE gene in Mycobacterium avium subsp. hominissuis (MAH), using 334 MAH isolates obtained from different isolation sources (222 human isolates, 145 Japanese and 77 Korean; 37 bathroom isolates; and 75 pig isolates). In total, 31 single-nucleotide polymorphisms (SNPs), which consisted of 16 synonymous SNPs and 15 nonsynonymous SNPs, were determined through comparison with the MACPPE12 gene sequence of MAH strain 104 as a reference. As the result, the 334 MAH isolates were classified into 19 and 13 different sequevars at the nucleic acid level (NA types) and amino acid level (AA types), respectively. Among the 13 AA types, only one type, the AA02 type, presented various NA types (7 different types) with synonymous SNPs, whereas all other AA types had a one-to-one correspondence with the NA types. This finding suggests that AA02 is a longer discernible lineage than the other AA types. Therefore, AA02 was classified as an ancestral type of the MACPPE12 gene, whereas the other AA types were classified as modern types. The ubiquitous presence of AA02 in all of the isolation sources and all different sequevars classified by the hsp65 genotype further supports this classification. In contrast to the ancestral type, the modern types showed remarkable differences in distribution between human isolates and pig isolates, and between Japanese isolates and Korean isolates. Divergence of the MACPPE12 gene may thus be a good indicator to characterize MAH strains in certain areas and/or hosts.


Tsubata Y.,The University of Shimane | Sutani A.,The University of Shimane | Okimoto T.,The University of Shimane | Matsuura M.,Hiroshima City Hospital | And 10 more authors.
Anticancer Research | Year: 2012

Background: Pleomorphic carcinoma (PC) of the lung is a rare tumor that usually has an aggressive clinical course and a poor prognosis. In this study, 75 cases of PC were reviewed to identify its clinical features, and we examined the expression of angiogenic factors. Patients and Methods: We immunohistochemically examined the expression of angiogenic factors in tissue specimens of PC. Results: 66 males and 9 females were examined. The median survival time was 16.5 months. The stage and symptomatical diagnosis were significantly associated with the survival. In the immunohistochemical analyses, vascular endothelial growth factor (VEGF) was expressed in many cases of PC. A high score for angiogenesis was significantly related to a poorer prognosis. Conclusion: We conclude that PC should be considered an aggressive disease, and that the stage and symptomatical diagnosis are strong prognostic factors. Furthermore, tumor angiogenesis provides significant prognostic information about the clinical outcome in PC.


Kobashi Y.,Kawasaki Medical School | Abe T.,National Ehime Hospital | Shigeto E.,National Hiroshima Hospital | Yano S.,National Matsue Hospital | And 2 more authors.
Internal Medicine | Year: 2010

Objective We retrospectively evaluated the clinical usefulness of desensitization therapy for many patients showing allergic reactions to anti-mycobacterial drugs (INH and RFP) according to the proposition reported by the Japanese Society for Tuberculosis (JST). Methods Desensitization therapy for anti-mycobacterial drugs was performed according to the propositions of JST for forty-six patients with mycobacterial disease in several hospitals partcipating in the Chugoku- Shikoku Mycobacterial Disease Committee between January 1999 and December 2009. Results Adverse reactions occurred as drug-induced skin eruptions in 23 patients, drug-induced fever in 16, and drug-induced fever plus eruption in 7. The causative drugs suggested by the clinical course or DLST were RFP in 30 patients and INH in 16. The clinical effects of desensitization therapy for individual drugs was good in 23 of 30 patients (77%) receiving RFP, and in 13 of 16 (81%) receiving INH. Ten patients showing failure of desensitization included 5 elderly patients and 2 patients with a history of drug allergies. The interval until initiation of desensitization therapy ranged from 5 to 30 days after the disappearance of adverse reactions and the interval until the appearance of adverse reactions during desensitization therapy ranged from 3 to 18 days. A comparative study between the patient group with successful desensitization therapy and that with failure of desensitization did not show any significant differences except for the interval until initiation of desensitization therapy. Conclusion We confirmed the clinical effectiveness of desensitization therapy for anti-mycobacterial drugs according to the propositions of JST in this multicenter study. © 2010 The Japanese Society of Internal Medicine.


Nakasaki N.,Yoshijima Hospital | Harada Y.,Hiroshima University
Japanese Journal of Clinical Ophthalmology | Year: 2012

Purpose: To report two cases of suprachoroidal hemorrhage that developed after trabeculectomy. Silicone oil tamponade was eventually effective in both cases. Cases: Both were males and were aged 66 and 79 years respectively. One case had received cataract surgery during infancy. The other had been treated by peroral anticoagulant for artificial pace maker. Both underwent trabeculectomy for glaucoma. Subchoroidal hemorrhage developed on day 2 and 9 respectively. Removal of blood through sclerectomy was followed by recurrence of subchoroidal hemorrhage. Final cure was obtained after vitrectomy and silicone oil tamponade. Conclusion: These cases illustrate that vitrectomy with silicone oil tamponade may be effective for recurrent subchoroidal hemorrhage after trabeculectomy.


Tsubata Y.,The University of Shimane | Sutani A.,The University of Shimane | Okimoto T.,The University of Shimane | Murakami I.,Higashi hiroshima Medical Center | And 9 more authors.
Anticancer Research | Year: 2015

Background/Aim: Pleomorphic carcinoma (PC) of the lung is a rare tumor that usually has an aggressive clinical course and a poor prognosis. Clinical and pathological features remain unclear. The aim of this study was to determine whether tumor angiogenesis of PC is upregulated compared to that in adenocarcinoma (AD). Materials and Methods: We collected 55 cases of PC and AD in which the patients had undergone either lung resection or autopsy and immunohistochemically examined the expression of vascular endothelial growth factor (VEGF), hypoxia-inducible factor (HIF)-1α and microvessel density (MVD) in tissue specimens.Results: VEGF was expressed in many cases of both PC and AD with no significant differences between the groups. In contrast, the expression of HIF-1α and MVD were significantly greater in PC than AD. Median survival time of the PC group was 14.7 months and significantly shorter than that of the AD group.Conclusion: MVD and expression of HIF-1α are associated with angiogenesis in PC and confer a poorer prognosis. Tumor angiogenesis provides significant prognostic information regarding clinical outcome in patients with PC.


Yamashita Y.,Yoshijima Hospital | Maeda S.,Yoshijima Hospital | Sakatani T.,Yoshijima Hospital | Sakashita Y.,Yoshijima Hospital
Journal of the Japan Diabetes Society | Year: 2012

Whether diabetes mellitus (DM) is associated with nontuberculous mycobacterial lung disease (NTMLD) remains unknown. DM is suspected to be a risk factor of NTMLD similar to pulmonary tuberculosis because of its immunosuppressive effect. To clarify the effect of DM on NTMLD, we first investigated the prevalence of DM among NTMLD patients and then compared characteristics of DM patients (those with HbA1c (JDS value) ≧6.1% and receiving treatment for diabetes) with those of non-DM patients. Subjects included 103 patients hospitalized between January 2005 and December 2007 for treatment or examination of NTMLD. Prevalence of diabetes among all patients was 26.2%, 38.8% in men and 14.8% in women. The prevalence of diabetes in NTMLD patient was significantly higher in men than in women. Additionally, the prevalence of diabetes in male NTMLD patients was higher than in the general Japanese male population. These results suggest that diabetes is associated with NTMLD in men.

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