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Watanabe K.,Toho University | Ikeda M.,Yokosuka General Hospital Uwamachi | Takahashi M.,Yokohama Municipal Citizens Hospital | Miki K.,Japan Research Foundation of Prediction | And 2 more authors.
Journal of the Medical Society of Toho University | Year: 2010

Background: Although the use of gene expression profiling by DNA microarray analysis in the molecular diagnosis of gastric cancer has been studied, its daily clinical application has not been successful. We attempted to prepare a practical gene diagnostic system and clarify marker genes for gastric cancer cell lines and tissues. Methods: We prepared an original DNA microarray with 338 genes selected as gastric cancer-related genes. Then, a DNA microarray and/or real-time polymerase chain reaction (PCR) was used to examine gene expression in 5 gastric cancer-derived cell lines (2 well differentiated cell lines and 3 poorly differentiated cell lines) and 17 paired tissues of patients with gastric cancer. The gene network was analyzed by Ingenuity Pathways Analysis. Results: Seven differentially expressed genes were identified between well differentiated and poorly differentiated gastric cancer cell lines, and 14 differentially expressed genes were identified between gastric cancer tissues and noncancerous tissues from sites adjacent to tumors. COL4A1 and PLS3 may be new markers for gastric cancer. Conclusions: Differentially expressed genes were observed in gastric cancer cell lines and tissues. COL4A1 and PLS3 were possible new markers for gastric cancer.

Osawa T.,Yokosuka General Hospital Uwamachi
Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society | Year: 2011

A 69-year-old man who had had a history of abnormal chest radiograph was admitted. Chest computed tomography showed an 18 x 18 mm apparently-circumscribed mass in the right posterior-basal segment. We performed right lower lobe wedge resection via video-assisted thoracic surgery to establish a definitive diagnosis. The mass was an apparently-circumscribed, gray and solid tumor, the microscopic findings of which included diffuse proliferation of plasma cells with an abnormal profile. Immunohistochemical staining revealed IgG-kappa light chain monoclonality. Absence of M protein in the serum and urine was confirmed. Bone-marrow puncture and positron-emission tomography revealed normal findings. Thus a diagnosis of extramedullary plasmacytoma (EMP) of the lung was made. We report trends of the immunoglobulin isotype of EMP in Japan. Since it has been reported that some of the cases in EMP progress to multiple myeloma (MM), careful observation and follow-up is needed hereafter. In the present case, no recurrence or progress to MM was observed within the first year after surgery.

Nishimoto H.,Yokosuka General Hospital Uwamachi | Nishimoto H.,Kitasato University | Takahashi Y.,Aichi Medical University | Kakizaki H.,Aichi Medical University
Ophthalmic Plastic and Reconstructive Surgery | Year: 2013

PURPOSE:: To examine the relationship of horizontal lower eyelid laxity, frequency of involutional entropion occurrence, and age of Asian patients. METHODS:: Three hundred seventy-six Japanese patients were examined (age range, 3-95 years). The horizontal laxity was examined with a pinch test. A pinch test of >8 mm was judged as positive. The percentage of entropion eyelids was calculated, and pinch tests were also performed. The percentage of entropion eyelids in the total positive pinch test eyelids was calculated. RESULTS:: A positive pinch test first appeared around 40 years of age and increased with age: 7.4% at 50 to 59 years, 8.5% at 60 to 69 years, 18.1% at 70 to 79 years, 37.9% at 80 to 89 years, and 50.0% at ≥90 years. The entropion first appeared around 60 years of age. All patients demonstrated unilateral entropion with a positive pinch test. The percentage of entropion occurrence showed an increase with age: 1.7% at 60 to 69 years, 2.6% at 70 to 79 years, 5.2% at 80 to 89 years, and 8.3% at ≥90 years. The percentage of entropion eyelids in the total positive pinch test eyelids was 20.0% at 60 to 69 years, 14.3% at 70 to 79 years, 13.6% at 80 to 89 years, and 16.7% at ≥90 years, and no significant difference was found among all ages (p = 0.985). CONCLUSIONS:: Lower eyelid horizontal laxity and involutional entropion occurrence increased with age, and the latter was similar over ages when the pinch test was >8 mm. © 2013 The American Society of Ophthalmic Plastic and Reconstructive Surgery, Inc..

Fujimoto N.,Okaama Rosai Hospital | Aoe K.,Yamaguchi Ube Medical Center | Oizumi S.,Hokkaido University | Kozuki T.,Shikoku Cancer Center | And 4 more authors.
Japanese Journal of Lung Cancer | Year: 2014

Objective. We evaluated the usefulness of assessing the hyaluronic acid (HA) level in pleural fluid for the differential diagnosis of malignant pleural mesothelioma (MPM).Method. The data regarding the pleural fluid HA concentration were retrospectively collected from Rosai Hospitals and related facilities in Japan.Reult. A total of 860 cases were examined, which included 139 cases of MPM, 76 of benign asbestos pleurisy (BAP), 324 of lung cancer (LC), 74 of other malignant conditions (OMC), 120 of infectious pleuritis (IP), 11 of collagen diseases (CD) and 116 cases had other conditions. The median (range) HA concentrations in the pleural fluid were 76,650 (211-33,000,000) ng ml in the MPM cases, 28,000 (165-152,000) ng ml in the BAP, 19,000 (800-134,000) ng ml in the LC, 12,200 (900-157,200) ng ml in the OMC, 23,400 (900-230,000) ng ml in the IP, 17,800 (9,000-80,800) ng ml in the CD and 11,575 (23-90,000) ng ml in patients with other diseases. The HA levels were significantly higher in MPM cases than in the patients with other diseases. The receiver operating characteristics (ROC) analysis revealed an area under the ROC curve value of 0.818 (95% confidence interval, 0.772-0.864) for the differential diagnosis of MPM. With a cut-off value of 100,000 ng ml, the sensitivity was 44.5% and the specificity was 98.2%. These results indicate that MPM should be strongly suspected in cases with an elevated concentration of pleural fluid HA.Conclusion. The pleural fluid HA concentrations might be useful for the differential diagnosis of MPM. © 2014 The Japan Lung Cancer Society.

Yuyama K.,Yokosuka General Hospital Uwamachi | Matsushita H.,Yokosuka General Hospital Uwamachi | Egawa K.,Yokosuka General Hospital Uwamachi | Osawa T.,Yokosuka General Hospital Uwamachi | And 3 more authors.
Japanese Journal of Cancer and Chemotherapy | Year: 2012

Erlotinib is a selective epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor, which shows favorable antitumor activity against chemorefractory lung carcinoma, especially with EGFR gene mutations. We experienced a case in which oxygenation and performance status improved in with low-dose erlotinib of 100 mg/day administered every other day incidentally, although a dose of erlotinib 150 mg/day daily is encouraged by the RECIST guideline. A 77-year-old male was diagnosed with bronchioloalveolar carcinoma (BAC) and given first-line combination chemotherapy of carboplatin and pemetrexed. However, antitumor activity was not satisfactory. His performance status (PS) was scored as 2 and home oxygen therapy (HOT) was introduced. As second-line chemotherapy, erlotinib was administered 100 mg/day daily. However, the patient took the medication every other day at his own discretion, due to severe eruption and itching sensation. Since this case is classified as a nonmeasurable lesion according to the RECIST guideline, it was difficult to measure antitumor activity by tumor size. But improvement of oxygenation, with a performance status score of 0 and normalization of serum CEA level suggested that erlonitib administration of 100 mg/day every other day showed antitumor activity.

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