Yokohama, Japan
Yokohama, Japan

Yokohama City University is a public university, in Yokohama, Japan. As of 2013, YCU has two faculties with a total of around 4,850 students, 111 of whom are foreign. YCU also has four campuses and two hospitals . YCU is a member of the Port-City University League , and a core member of the Japanese University Network in the Bay Area . Wikipedia.


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Patent
Yokohama City University and Saitama University | Date: 2015-02-26

The present invention finds out find out the requirements necessary for preparing a cell condensate in vitro from a large number of cells (several ten thousand to several million cells) and provides a method of forming a cell condensate for self-organization which is capable of realizing complex higher structures (such as liver and kidney) and interactions with other organs. A method of preparing a cell condensate in vitro, comprising culturing a mixture of cells and/or tissues of a desired type in a total cell count of 400,000 or more and 100,000 to 400,000 mesenchymal cells to form a cell condensate of 1 mm or more in size. A cell condensate prepared by the above-described method. A method of preparing a three-dimensional tissue structure, comprising allowing self-organization of a cell condensate prepared by the above-described method to form a three-dimensional tissue structure that has been provided with higher structures. A gel-like support wherein the side on which culture is to be performed has a U- or V-shaped cross-section.


Patent
Yokohama City University and Saitama University | Date: 2017-02-01

The present invention finds out find out the requirements necessary for preparing a cell condensate in vitro from a large number of cells (several ten thousand to several million cells) and provides a method of forming a cell condensate for self-organization which is capable of realizing complex higher structures (such as liver and kidney) and interactions with other organs. A method of preparing a cell condensate in vitro, comprising culturing a mixture of cells and/or tissues of a desired type in a total cell count of 400,000 or more and 100,000 to 400,000 mesenchymal cells to form a cell condensate of 1 mm or more in size. A cell condensate prepared by the above-described method. A method of preparing a three-dimensional tissue structure, comprising allowing self-organization of a cell condensate prepared by the above-described method to form a three-dimensional tissue structure that has been provided with higher structures. A gel-like support wherein the side on which culture is to be performed has a U- or V-shaped cross-section.


Patent
Kuraray Co. and Yokohama City University | Date: 2017-04-05

The present invention provides a culture method for culturing, in recesses (10), a population including two or more cells including a cell derived from a stem cell and a mesenchymal cell. The cell derived from a stem cell is a cell obtained by differentiating a stem cell in vitro. The cell is a cell of one or more types selected from the group consisting of an endodermal cell, an ectodermal cell, and a mesodermal cell. The population is cultured in the recesses (10) together with a vascular cell or a secretor factor. Each recess (10) includes a space in which cells are movable. When a volume of the space is represented by V mm^(3) and the number of mesenchymal cells seeded in the space is represented by N, V is 400 or less and NN is in a range from 35 to 3000.


We analyzed 1,900 Turkish Behçet's disease cases and 1,779 controls genotyped with the Immunochip. The most significantly associated SNP was rs1050502, a tag SNP for HLA-B*51. In the Turkish discovery set, we identified three new risk loci, IL1A–IL1B, IRF8, and CEBPB–PTPN1, with genome-wide significance (P < 5 × 10-8) by direct genotyping and ADO–EGR2 by imputation. We replicated the ADO–EGR2, IRF8, and CEBPB–PTPN1 loci by genotyping 969 Iranian cases and 826 controls. Imputed data in 608 Japanese cases and 737 controls further replicated ADO–EGR2 and IRF8, and meta-analysis additionally identified RIPK2 and LACC1. The disease-associated allele of rs4402765, the lead marker at IL1A–IL1B, was associated with both decreased IL-1α and increased IL-1β production. ABO non-secretor genotypes for two ancestry-specific FUT2 SNPs showed strong disease association (P = 5.89 × 10-15). Our findings extend the list of susceptibility genes shared with Crohn's disease and leprosy and implicate mucosal factors and the innate immune response to microbial exposure in Behçet's disease susceptibility. © 2017 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.


Patent
Yokohama City University and Nihon Medi Physics Co. | Date: 2017-05-17

A novel technique of quantifying nuclear medicine data is provided. The novel technique is characterized in that information acquired from nuclear medicine image data is normalized with bone mineral content (BMC) or bone mineral density (BMD). Some embodiments use, instead of the conventional SUV, the SUVbone that has been invented by the inventors of the present application such as the following: SUVbone = {Amount of attenuation-corrected radioactivity in region of interest (kBq) Volume of region of interest (ml)} / {Administered radioactivity dose (kBq) Bone mineral content (g)} or SUVbone = {Amount of attenuation-corrected radioactivity in region of interest (kBq) Volume of region of interest (ml)} / {Administered radioactivity dose (kBq) Bone mineral density (g/m^(2))}. BMC or BMD may be estimated from sex, age, height, or weight of a subject.


Patent
Yokohama City University | Date: 2016-10-18

The present invention provides a means for reconstituting tissues and organs having mature functions. A method of preparing a tissue or an organ, comprising coculturing an organ cell with a vascular endothelial cell and a mesenchymal cell, generating an organ bud, transplanting the organ bud into a non-human animal, and then isolating from the non-human animal the transplanted organ bud-derived tissue or organ.


Patent
KURARAY Co. and Yokohama City University | Date: 2015-05-29

The present invention provides a culture method for culturing, in recesses (10), a population including two or more cells including a cell derived from a stem cell and a mesenchymal cell. The cell derived from a stem cell is a cell obtained by differentiating a stem cell in vitro. The cell is a cell of one or more types selected from the group consisting of an endodermal cell, an ectodermal cell, and a mesodermal cell. The population is cultured in the recesses (10) together with a vascular cell or a secretor factor. Each recess (10) includes a space in which cells are movable. When a volume of the space is represented by V mm^(3 )and the number of mesenchymal cells seeded in the space is represented by N, V is 400 or less and N/V is in a range from 35 to 3000.


Patent
Yokohama City University, Osaka University and Nagoya University | Date: 2017-08-09

Means which enables preparation of a thick cell aggregate by a simple process without an operation of detaching and stacking of cells is disclosed. The method for preparing a three-dimensional cell aggregate by the present invention comprises: a cell encasing step of placing a cell suspension in a cell container; and a pressure application step of applying pressure to cells in the container. The cell encasing step and the pressure application step may be carried out a plurality of times. By the present invention, a thick, robust cell aggregate can be obtained by a simple operation of applying pressure to a cell suspension or a medium containing cells. Since the method does not require an operation of stacking a plurality of cell sheets, the cells are hardly damaged, and the conditions of the cells can be favorably maintained, so that the cells can be advantageously used as a tissue piece for transplantation.


Patent
Yokohama City University, Biomedica Solution Co. and Shibuya Corporation | Date: 2017-08-02

An operation isolator 21 forms an aseptic space. An incubator 33 is connected to the operation isolator 21, in which cells are stored and cultured. A storage chamber 52 stores articles used in the operation isolator 21. In order to carry articles from the outside into the storage chamber 52, a decontamination pass-box is provided. The storage chamber 52 and the operation isolator 21 are directly or indirectly connected to each other.


Patent
Yokohama City University, Osaka University and Nagoya University | Date: 2015-09-29

Means which enables preparation of a thick cell aggregate by a simple process without an operation of detaching and stacking of cells is disclosed. The method for preparing a three-dimensional cell aggregate by the present invention comprises: a cell encasing step of placing a cell suspension in a cell container; and a pressure application step of applying pressure to cells in the container. The cell encasing step and the pressure application step may be carried out a plurality of times. By the present invention, a thick, robust cell aggregate can be obtained by a simple operation of applying pressure to a cell suspension or a medium containing cells. Since the method does not require an operation of stacking a plurality of cell sheets, the cells are hardly damaged, and the conditions of the cells can be favorably maintained, so that the cells can be advantageously used as a tissue piece for transplantation.

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