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Wuxi, China

Shen L.,Nanjing Medical University | Du M.,Nanjing Medical University | Wang C.,Nanjing Medical University | Gu D.,Nanjing Medical University | And 10 more authors.
International Journal of Molecular Sciences | Year: 2014

This study aimed to investigate the association between POU class5 homeobox 1 pseudogene 1 gene (POU5F1P1) rs10505477 polymorphism and the prognosis of Chinese gastric cancer patients, who received cisplatin-based chemotherapy after surgical resection. POU5F1P1 rs10505477 was genotyped using the SNaPshot method in 944 gastric cancer patients who received gastrectomy. The association of rs10505477 G > A polymorphism with the progression and prognosis in gastric cancer patients was statistically analyzed using the SPSS version 18.0 for Windows. The results reveal that rs10505477 polymorphism has a negatively effect on the overall survival of gastric cancer patients in cisplatin-based chemotherapy subgroup (HR = 1.764, 95% CI = 1.069-2.911, p = 0.023). Our preliminary study indicates for the first time that POU5F1P1 rs10505477 is correlated with survival of gastric cancer patients who receving cisplatin-based chemotherapy after gastrectomy. Further studies are warranted to investigate the mechanism and to verify our results in different populations. © 2014 by the authors; licensee MDPI, Basel, Switzerland. Source


Zhang X.,Nanjing Medical University | Gu D.,Nanjing Medical University | Du M.,Nanjing Medical University | Wang M.,Nanjing Medical University | And 10 more authors.
International Journal of Molecular Sciences | Year: 2014

The orphan nuclear receptor (NR5A2), which belongs to the NR5A subfamily of nuclear receptors, is expressed in developing and adult tissues of endodermal origin, and can contribute to the development of several cancers through regulating cell proliferation. NR5A2 (rs3790843 and rs3790844) single nucleotide polymorphisms (SNPs) genotyping were examined in DNA samples, extracted from paraffin-embedded cancer tissue. Clinicopathologic and follow-up data were collected from 944 patients with gastric cancer (GC). Associations of the 2 SNPs with the progression and prognosis in gastric cancer patients were analyzed using the SPSS version 18.0. We found that NR5A2 rs3790843 polymorphism was significantly associated with the risk of GC which had regional lymph node metastasis (p = 0.044) or distant metastasis (p = 0.020). Our results also indicated that rs3790844 polymorphism was associated with the increased overall survival (OS) of GC patients in the dominant model (GG vs. GA/AA, HR (hazard ratio) = 0.823, 95% CI (confidence interval) = 0.679–0.997), suggesting a potential protective role of the variant A allele. Additionally, in the stratified analysis, both NR5A2 rs3790843 and rs3790844 polymorphism were associated with significantly lower risk of death in the groups of female, tumor size >5 cm in a dominant model. Our results represent the first demonstration that the NR5A2 rs3790844 polymorphism is associated with increased OS of GC patients in the dominant model, and similar results were found among the female group and tumor size >5 cm group for NR5A2 rs3790843 polymorphism. Further validation in other larger studies with different ethnic populations and functional evaluations are needed. © 2014 by the authors; licensee MDPI, Basel, Switzerland. Source


Gu H.-M.,Yixing Tumor Hospital
Molecular and Cellular Toxicology | Year: 2015

adix ranunculus temate saponins (RRTS), one of the main constituents extracted from the popular traditional Chinese medicine Radix Ranunculi ternati, has been reported to have various biological activities including anti-cancer effect. The aim of this study is to investigate the effect of RRTS on the cell proliferation and apoptosis in human gastric adenocarcinoma SGC-7901 cells. The data showed that exposure to RRTS for 24 h produced cytotoxic effects on SGC- 7901 cells in a dose-dependent manner (with an IC50 value of 21.22±2.76 μg/mL), which was accompanied by apoptosis induction (from 2.18±0.89% (control) to 63.72±13.16% (100 μg/mL)). Both the extrinsic or death receptor pathway and the intrinsic or mitochondrial pathway were involved in RRTS-induced apoptosis in SGC-7901 cells. Furthermore, apoptotic signaling induced by RRTS was amplified by cross-link between the two pathways via the signal-integrating protein Bid. In conclusion, our findings contribute to better understanding the molecular mechanism of RRTS’ effect on gastric cancer cells and form the basis of the therapeutic development of RRTS in treating gastric cancer in the future. © 2015, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht. Source


Wang S.,Nanjing Medical University | Tao G.,Nanjing Medical University | Wu D.,Nanjing Medical University | Zhu H.,Core Laboratory of Nantong Cancer Hospital | And 7 more authors.
Molecular Carcinogenesis | Year: 2013

Genetic variations in miRNAs have been demonstrated to be capable of altering miRNA expression, consequently affecting many cancer-related biological processes. The MIR196A2 rs11614913 (T>C) polymorphism has been reported to be associated with various cancers development and progression. In our study, we aim to explore whether this polymorphism is relevant to the genetic susceptibility and prognosis of gastric cancer in a Chinese population. We analyzed the correlations of rs11614913 polymorphism with gastric cancer susceptibility in test and validation sets. The test set comprised 749 cases and 900 controls, while the validation set enrolled 940 cases and 1046 controls. Moreover, we evaluated the association between the polymorphism and gastric cancer prognosis in the validation set with follow-up information. The variant rs11614913 CC genotype was associated with a significantly reduced risk of gastric cancer in both sets (adjusted odds ratio [OR]=0.78, 95% confidence interval [CI]=0.62-0.99 for the test set and 0.64, 0.52-0.80 for the validation set) compared with the CT/TT genotypes. Furthermore, the CC genotype was associated with a significantly increased survival of gastric cancer compared with the CT/TT genotypes (adjusted hazard ratio [HR]=0.72, 95% CI=0.55-0.95), and the association was more prominent among patients with non-cardia gastric cancer than those with cardia gastric cancer (adjusted HR=0.57, 95% CI=0.40-0.83 for NCGC and 1.00, 0.65-1.53 for CGC). Our results suggested that the genetic variation of MIR196A2 may play a role in gastric cancer tumorigenesis. © 2013 Wiley Periodicals, Inc. Source


Wang S.,Nanjing Medical University | Tian Y.,Nanjing Medical University | Wu D.,Nanjing Medical University | Zhu H.,Nanjing Medical University | And 6 more authors.
Mutagenesis | Year: 2012

Gastric cancer is the second leading cause of cancer-related death worldwide with a low 5-year survival (S5y) after initial diagnosis. Although aberrant Wnt/β-catenin (CTNNB1) signaling has been observed in multiple human cancers, there is no information on the role of CTNNB1 polymorphisms in gastric cancer risk and S5y. We performed a genetic association study to analyse the correlation between the five tagged SNPs (tSNPs) (rs4135385, rs1798808, rs1880481, rs11564465 and rs2293303) of CTNNB1 and gastric cancer risk and survival. A total of 944 patients with complete follow-up information and 848 cancer-free controls were enrolled in this study. The rs1880481 polymorphism was correlated with decreased risk of gastric cancer [AC/AA vs. CC: adjusted odds ratio (OR) = 0.76, 95% confidence interval (CI) = 0.63-0.91], whereas the three other SNPs showed opposite effect (AG/AA vs. GG: adjusted OR = 1.31, 95% CI = 1.08-1.57 for rs4135385; GG vs. AA/AG: 2.09, 1.02-4.28 for rs11564475; TT vs. CC/CT: 4.87, 2.72-8.71 for rs2293303). We further investigated if these tSNPs were related to the S5y of gastric cancer, and the results displayed that only the SNP rs4135385 AG/AA genotypes were significantly associated with a favorable gastric cancer survival compared with the GG genotype [adjusted hazard ratio (HR) = 0.80, 95% CI = 0.66-0.97], and the association was more prominent among patients with non-cardia gastric cancer (NCGC) than those with cardia gastric cancer (CGC) (Log-rank P = 0.007 for NCGC and 0.417 for CGC). Our results indicated that the genetic variants of CTNNB1 could be used as predictors of gastric cancer susceptibility and prognosis. © The Author 2012. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. Source

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