Yiwu City Central Hospital

Yiwu, China

Yiwu City Central Hospital

Yiwu, China
SEARCH FILTERS
Time filter
Source Type

Wang L.-X.,Changhai Hospital | Li J.-W.,Yiwu City Central Hospital | Huang J.-Y.,Yiwu City Central Hospital | Li J.-H.,Yiwu City Central Hospital | And 3 more authors.
Tumor Biology | Year: 2015

Chlorin derivatives are promising photosensitive agents for photodynamic therapy (PDT) of tumors. The aim of the current study is to investigate the PDT therapeutic effects of a novel chlorin-based photosensitizer, meso-tetra[3-(N,N-diethyl)aminomethyl-4-methoxy]phenyl chlorin (TMPC) for gliomas in vitro and in vivo. Physicochemical characteristics of TMPC were recorded by ultraviolet visible spectrophotometer and fluorescence spectrometer. The rate of singlet oxygen generation of TMPC upon photo-excitation was detected by using 1,3-diphenylisobenzofuran (DPBF). The accumulation of TMPC in gliomas U87 MG cells was measured by fluorescence spectrometer. The efficiency of TMPC-PDT in vitro was analyzed by MTT assay and clonogenic assay. The biodistribution and clearance of TMPC were determined by fluorescence measuring. Human gliomas U87 MG tumor-bearing mice model was used to evaluate the antitumor effects of TMPC-PDT. TMPC shows a singlet oxygen generation rate of 0.05 and displays a characteristic long wavelength absorption peak at 653 nm (ε = 15,400). The accumulation of TMPC increased with the increase of incubation time. In vitro, PDT using TMPC and laser showed laser dose- and concentration-dependent cytotoxicity to U87 MG cells. In U87 MG tumor-bearing mice, TMPC-PDT significantly reduced the growth of the tumors. Both in vitro and in vivo, TMPC showed little dark toxicity. In vitro and in vivo studies, it found that TMPC has excellent antitumor activities. It suggests that TMPC is a potential photosensitizer of photodynamic therapy for cancer. © 2015, International Society of Oncology and BioMarkers (ISOBM).


PubMed | Yiwu City Central Hospital, Changhai Hospital, University College Dublin and Donghua University
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2015

Chlorin derivatives are promising photosensitive agents for photodynamic therapy (PDT) of tumors. The aim of the current study is to investigate the PDT therapeutic effects of a novel chlorin-based photosensitizer, meso-tetra[3-(N,N-diethyl)aminomethyl-4-methoxy]phenyl chlorin (TMPC) for gliomas in vitro and in vivo. Physicochemical characteristics of TMPC were recorded by ultraviolet visible spectrophotometer and fluorescence spectrometer. The rate of singlet oxygen generation of TMPC upon photo-excitation was detected by using 1,3-diphenylisobenzofuran (DPBF). The accumulation of TMPC in gliomas U87 MG cells was measured by fluorescence spectrometer. The efficiency of TMPC-PDT in vitro was analyzed by MTT assay and clonogenic assay. The biodistribution and clearance of TMPC were determined by fluorescence measuring. Human gliomas U87 MG tumor-bearing mice model was used to evaluate the antitumor effects of TMPC-PDT. TMPC shows a singlet oxygen generation rate of 0.05 and displays a characteristic long wavelength absorption peak at 653 nm (=15,400). The accumulation of TMPC increased with the increase of incubation time. In vitro, PDT using TMPC and laser showed laser dose- and concentration-dependent cytotoxicity to U87 MG cells. In U87 MG tumor-bearing mice, TMPC-PDT significantly reduced the growth of the tumors. Both in vitro and in vivo, TMPC showed little dark toxicity. In vitro and in vivo studies, it found that TMPC has excellent antitumor activities. It suggests that TMPC is a potential photosensitizer of photodynamic therapy for cancer.


PubMed | Yiwu City Central Hospital and Zhejiang Provincial Peoples Hospital
Type: | Journal: Journal of clinical laboratory analysis | Year: 2017

To discover how NLRP3 and TNFRSF1A polymorphisms affect the efficacy of traditional medicine and etanercept for ankylosing spondylitis (AS) patients.Single nucleotide polymorphism (SNP) and haplotype analyses were conducted based on determined NLRP3 and TNFRSF1A among AS patients. We subsequently analyzed the relationship between relevant clinical indexes and polymorphisms of NLRP3 and TNFRSF1A.The 4 SNP loci on NLRP3 and 3 SNP loci on TNFRSF1A showed significant linkage disequilibrium, respectively. The T allele of NLRP3 rs4612666 and the T allele of TFRSF1A rs4149570 are both associated with AS (P<.05). The T-A-C-T haplotype of NLRP3 as well as the G-C-C, T-C-C, T-C-T, and T-T-T haplotypes of TFRSF1A are associated with AS (P<.05). The morning stiffness time, BASDAI scoring, and ESR of patients receiving etanercept were significantly higher than those receiving traditional medicine. T allele of NLRP3 rs4612666 had a significantly greater negative impact on the ASAS20 improvement than C allele. Whereas the A allele of NLRP3 rs3806268 had a significantly greater positive impact on the ASAS20 improvement than G allele. There is no significant association between SNP and efficacy of traditional medicine in the treatment of AS.NLRP3 and TFRSF1A (rs4149570) are associated with AS susceptibility. There is a significant association between NLRP3 polymorphisms and treatment of etanercept.


Ye Y.,Donghua University | Yan Y.J.,Donghua University | Chen Z.L.,Donghua University | Li J.W.,Yiwu City Central Hospital | O'Shea D.,University College Dublin
Material Science and Environmental Engineering - Proceedings of the 3rd annual 2015 International Conference on Material Science and Environmental Engineering, ICMSEE 2015 | Year: 2016

This study was designed to investigate the efficacy of Photodynamic Therapy (PDT) in treating esophageal cancer in a preclinical study. Pyropheophorbide a (PPa), a photosensitizer, was tested on Human Esophageal Carcinoma Cell Eca-109 in culture. The cell photo damage occurred since the time the concentrations of PPa reached 0.01 μM (20 J/cm2). The in vivo distribution of PPa (30 mg/kg, intraperitoneal) was investigated on Eca-109 tumor-bearing nude mice. The tumor vs skin concentration ratio was 2.7:1 at 24 h. Our results showed that PPa was an effective photosensitizer with good photo toxicity and tumor targeting distribution. © 2016 Taylor and Francis Group, London.


Ye Y.,Donghua University | Yan Y.J.,Donghua University | Chen Z.L.,Donghua University | Li J.W.,Yiwu City Central Hospital | Margetic D.,Ruder Boskovic Institute
Material Science and Environmental Engineering - Proceedings of the 3rd annual 2015 International Conference on Material Science and Environmental Engineering, ICMSEE 2015 | Year: 2016

The effectiveness of PDT depends strongly on the type of photosensitizers. Hematoporphyrin Monomethyl Ether (HMME) is a promising second-generation porphyrin-related photosensitizer for PDT. HMME-PDT-induced cell death and its mechanisms were investigated in human hepatoma cell SMMC-7721. The survival rate of SMMC-7721 cells determined by MTT assay decreased with the increasing HMME concentration and laser dose. The expression level of TNFSF13, a member of tumor necrosis factor superfamily, increased significantly. These results imply that photodynamic therapy with HMME may therefore be a useful clinical treatment for liver cancer. © 2016 Taylor and Francis Group, London.


Li J.-W.,Yiwu City Central Hospital | Wu Z.-M.,Yiwu City Central Hospital | Magetic D.,Ruder Boskovic Institute | Zhang L.-J.,Donghua University | Chen Z.-L.,Donghua University
Tumor Biology | Year: 2015

In this paper, the antitumor activity of a novel porphyrin-based photosensitizer 5,10,15,20-tetrakis[(5-diethylamino)pentyl] porphyrin (TDPP) was reported in vitro and in vivo. The photophysical and cellular properties of TDPP were investigated. The singlet oxygen generation quantum yield of TDPP was detected; it showed a high singlet oxygen quantum yield of 0.52. The intracellular distribution of photosensitizer was detected with laser scanning confocal microscopy. The efficiency of TDPP-photodynamic therapy (PDT) in vitro was analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and in situ trypan blue exclusion test. Treated with a 630-nm laser, TDPP can kill cultured human esophageal cancer cell line (Eca-109) cells and reduce the growth of Eca-109 xenograft tumors significantly in BABL/c nude mice. And histopathological study was also used to confirm the antitumor effect. It has the perspective to be developed as a new antitumor drug in photodynamic therapy and deserves further investigation. © 2015, International Society of Oncology and BioMarkers (ISOBM).


PubMed | Yiwu City Central Hospital, Ruder Boskovic Institute and Donghua University
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2015

In this paper, the antitumor activity of a novel porphyrin-based photosensitizer 5,10,15,20-tetrakis[(5-diethylamino)pentyl] porphyrin (TDPP) was reported in vitro and in vivo. The photophysical and cellular properties of TDPP were investigated. The singlet oxygen generation quantum yield of TDPP was detected; it showed a high singlet oxygen quantum yield of 0.52. The intracellular distribution of photosensitizer was detected with laser scanning confocal microscopy. The efficiency of TDPP-photodynamic therapy (PDT) in vitro was analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and in situ trypan blue exclusion test. Treated with a 630-nm laser, TDPP can kill cultured human esophageal cancer cell line (Eca-109) cells and reduce the growth of Eca-109 xenograft tumors significantly in BABL/c nude mice. And histopathological study was also used to confirm the antitumor effect. It has the perspective to be developed as a new antitumor drug in photodynamic therapy and deserves further investigation.

Loading Yiwu City Central Hospital collaborators
Loading Yiwu City Central Hospital collaborators