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Shao M.,Linyi Peoples Hospital | Xu P.,Linyi Yishui Central Hospital | Liu J.,Binzhou Medical College | Liu W.,Linyi Peoples Hospital | Wu X.,Linyi Peoples Hospital
Patient Preference and Adherence | Year: 2016

Background: Bacterial meningitis is a serious infection in children and adults worldwide, with considerable morbidity, mortality, and severe neurological sequelae. Dexamethasone is often used before antibiotics in cases of this disease, and improves outcomes. Objective: Although several studies have identified the role of adjunctive dexamethasone therapy in the treatment of bacterial meningitis, the results are still inconclusive. The aim of this study was to systematically evaluate the therapeutic and adverse effect of adjunctive dexamethasone in patients with bacterial meningitis. Materials and methods: Relevant randomized, double-blind, placebo-controlled trials of dexamethasone in bacterial meningitis published between 2000 and 2016 were retrieved from the common electronic databases. The odds ratio (OR) and risk ratio (RR) with their 95% confidence interval (CI) were employed to calculate the effect. Results: A total of ten articles including 2,459 bacterial meningitis patients (1,245 in the dexamethasone group and 1,214 in the placebo group) were included in this meta-analysis. Our result found that dexamethasone was not associated with a significant reduction in follow-up mortality (292 of 1,245 on dexamethasone versus 314 of 1,214 on placebo; OR =0.91, 95% CI =0.80-1.03, P=0.14) and severe neurological sequelae (22.4% versus 24.1%, OR =0.84, 95% CI =0.54-1.29, P=0.42). However, dexamethasone seemed to reduce hearing loss among survivors (21.2% versus 26.1%; OR =0.76, 95% CI =0.59-0.98, P=0.03). No significant difference was found between these two groups in adverse events. Conclusion: Our results suggested that adjunctive dexamethasone might not be beneficial in the treatment of bacterial meningitis. Future studies with more data are needed to further prove the role of dexamethasone in bacterial meningitis. © 2016 Shao et al.

Weng H.-L.,Linyi Yishui Central Hospital | Wang M.-J.,Linyi Yishui Central Hospital
Molecular Medicine Reports | Year: 2016

The aim of the present study was to investigate the effects of microRNA-338-3p (miR-338-3p) on morphine (MP)-induced apoptosis, and its underlying mechanisms. Freshly-isolated mouse peritoneal macrophages were cultured in vitro and treated with MP following transfection with miR-338-3p mimic, inhibitor or controls. miR-338-3p expression levels increased significantly following MP treatment (P<0.01). This increase was enhanced following transfection with miR-338-3p mimic (P<0.05) and abrogated following transfection with miR-338-3p inhibitor (P<0.05). The apoptotic rate increased significantly in groups treated with MP (P<0.05); however, this increase was abrogated by transfection with miR-338-3p inhibitor (P<0.05). Bioinformatics software predicted that sex determining region Y-box 4 (SOX4) was the target gene of miR-338-3p and this was verified using a dual-luciferase reporter gene system. SOX4 mRNA and protein expression levels decreased significantly following MP treatment (P<0.05); however, this decrease was abrogated following transfection with miR-338-3p inhibitor (P<0.05). Caspase-3 protein expression levels increased markedly following MP treatment (P<0.05); however, this increase was inhibited by transfection with miR-338-3p inhibitor (P<0.05). Therefore, decreased expression of miR-338-3p may suppress MP-induced apoptosis, potentially via the upregulation of SOX4 expression and the caspase-3-dependent apoptotic signaling pathway.

Xu M.,Linyi Yishui Central Hospital | Wang H.-F.,Linyi Yishui Central Hospital | Zhang H.-Z.,Anqiu Peoples Hospital
Asian Pacific Journal of Cancer Prevention | Year: 2015

Objective: To explore the expression of RECK and relevant matrix metalloproteinases (MMPs) in hepatoblastoma (HB) and neuroblastoma (NB) and their clinical significance in the tumor metastasis. Materials and Methods: Forty-five wax-stone samples of HB and 43 wax-stone samples of NB removed by surgical resection and confirmed by pathology in Linyi Yishui Central Hospital were selected. According to presence and absence of metastasis, both NB and HB samples were divided into metastatic group and non-metastatic group, namely NB metastatic group (n=28), NB non-metastatic group (n=15), HB metastatic group (n=15) and HB non-metastatic group (n=30). The expression of RECK, membrane type-1 matrix metalloproteinase (MT1-MMP) in HB tissue and RECK, MMP-14 in NB tissue was detected using immunohistochemical method, and the correlation between RECK and MT1-MMP, MMP-14 was analyzed. Results: The metastatic rate of NB was dramatically higher than that of HB, with statistical significance (P=0.003). The positive rate of RECK expression in NB group (30.2%) was slightly lower than in HB group (40.0%), but no significant difference was presented (P=0.338). The positive rate of MMPs expression in NB metastatic group was evidently higher than in HB metastatic group (P=0.024). The results of Spearman correlation analysis revealed that the expression of RECK in HB and NB tissues had a significantly-negative correlation with MT1-MMP and MMP-14, respectively (r=-0.499, P=0.012; r=-0.636, P=0.000). Conclusions: In HB and NB tissues, RECK is expressed lowly, while relevant MMPs highly, and RECK inhibits the tumor invasion and metastasis through negative regulation of relevant MMPs.

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