Yinzhou the Second Hospital

Ningbo, China

Yinzhou the Second Hospital

Ningbo, China

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Wu K.-W.,Yinzhou the Second Hospital | Chen L.,Fudan University | Gu W.,Fudan University
Fudan University Journal of Medical Sciences | Year: 2010

Objective To establish an effective rat model of middle cervical sympathetic ganglion block. Methods A total of 18 male SD rats were used in this study, 6 rats were randomly selected for the anatomy of middle sympathetic ganglia ; another 6 were used for simulated blockade of the middle cervical sympathetic ganglion by dorsally injecting 0. 4 mL ink via the interval of the first and second costotransverse joints, where the spinal process of the second thoracic vertebra was employed as a marker to show the range of infiltration; and the remaining 6 were used for blocking middle cervical sympathetic ganglion with 0. 4 mL of 0. 25% bupicaine. Results There was a middle sympathetic ganglion with a length of 3. 5 - 5 mm on both left and right sides, lying in front of the first and second costotransverse joints and deeply below subclavicular artery and vein. The middle cervical ganglia with simulated blockade were completely infiltrated by ink. Horner sign appeared on the blocked sides within 1-2 min in all rats injected with bupicaine, which lasted for 2 - 2. 5 h. Conclusions The method of middle cervical ganglion blockage we developed is effective, convenient, accurate and safe, which takes advantage of the spinous process of the second thoracic vertebra as a bony marker and administers bupicaine directly into the middle cervical ganlion via the interval of the first and second costotransverse joints.

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