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Dou H.,Yidu Central Hospital of Weifang | Tang S.,Jinan University
Turkish Neurosurgery | Year: 2014

AIm: To determine whether nucleotomy may aggravate lumbar degeneration at the operated level. Mat erIal and Methods: A normal healthy finite element model, two degeneration models and corresponding nucleotomy models of the L4-5 segment were created. Combined with 800 N preloading, 10 Nm moment was applied on the L4 superior endplate to simulate extension, flexion, left axial rotation and lateral bending, respectively. The shear stress of annulus fibrosus, posterior annulus bulge along with the contact force of facet joint at L4-5 were investigated. Result s: In all loading directions, the shear stress and contact force of the facet joint in two nucleotomy models were higher than those in mildly or moderately degenerative models, while the posterior annulus bulge was lower than those in mildly or moderately degenerative models. ConclusIon: Nucleotomy may adversely aggravate lumbar degeneration at the operated level.

Huan C.,Shandong University | Cui G.,Yidu Central Hospital of Weifang | Ren Z.,Shandong Normal University
Pakistan journal of pharmaceutical sciences | Year: 2015

A substantial proportion of acromegalic patients have co-existent hyperprolactinaemia. To compare this group of population (AC+HPRL) to those of patients with merely hyperprolactinemia (HPRL), a retrospective analysis of patients was conducted. Data regarding clinical and immunohistochemical features, and outcome for patients were reviewed throughout the follow-up period. Four hundred and twenty-three patients were enrolled, with 329 in patients with HPRL and 94 in patients with AC+HPRL. Patients in the AC+HPRL group had a younger age at diagnosis (38.13 ± 13.31 vs. 41.95 ± 14.70 years; p=0.025) and a higher rate of invasion (p=0.007) than those in the HPRL group. The AC+HPRL group had higher GH levels but relatively lower PRL levels than the HPRL group before and after surgery. The rates of positive staining for GH and PRL in HPRL group were 15.20% and 93.01%, and the rates in AC+HPRL group were 84.04% and 87.23%. Patients with HPRL had a lower recurrence rate compared with patients in AC+HPRL group (p=0.018). Additionally, there were no significant correlations between the mean degree of preoperative GH or PRL and the positive rates of immunostaining (p>0.05, p>0.05). The Ki-67 indexes in HPRL group and AC+HPRL group were 3.07% ± 2.13 and 2.33% ± 1.71, respectively (p=0.001). In conclusion, acromegalic patients with hyperprolactinemia need careful and long-term follow-up following an operation.

Zhang Q.-B.,Shandong University | Chen L.-M.,Shandong University | Li M.,Shandong University | Cui Y.-Q.,Shandong University | And 2 more authors.
American Journal of Translational Research | Year: 2016

Objective: Chronic kidney disease (CKD) predicted a poor prognosis in patients with coronary artery disease. There is a paucity of data on outcomes after revascularization in patients with chronic total occlusion (CTO) and CKD. This study aims to investigate the impact of CKD on the revascularization of CTO. Methods: This study enrolled 1,092 CTO patients received treatments in our hospital between February 2009 and January 2014. Major adverse cardiac and cerebrovascular events (MACCE) and all-cause mortality were compared to evaluate medium-and long-term outcomes. Median follow-up was 39 months (interquartile range, 27-52 months). Result: CKD decreased cumulative MACCE-free survival rate (54.4 ± 6.2% vs. 70.9 ± 2.5%, P < 0.001) and cumulative survival rate (68.6 ± 6.3% vs. 90.5 ± 1.6%, P < 0.001). Revascularization was associated with better outcomes among patients with (MACCE-free survival rate: 64.8 ± 5.7% vs. 20.1 ± 15.3%, P = 0.009; survival rate 78.4 ± 5.6% vs. 38.7 ± 17.4%, P = 0.006) or without CKD (MACCE-free survival rate 73.9 ± 2.7% vs. 61.0 ± 5.4%, P = 0.001; survival rate 92.9 ± 1.5% vs. 83.8 ± 4.0%, P = 0.009). The benefit from revascularization was attenuated by CKD. Compared with percutaneous coronary intervention (PCI), coronary artery bypass grafting (CABG) had similar cumulative survival rates among patients, whether with or without CKD, but was associated with a higher cumulative MACCE-free survival rate (80.5 ± 3.4% vs. 68.5 ± 4.0%, P = 0.017) among patients without CKD. Conclusion: CKD attenuated the benefit from revascularization for CTO. Moreover, CABG was not superior to PCI among CTO patients, but with a reduction in MACCE in patients without CKD. © 2016, E-Century Publishing Corporation. All rights reserved.

Zhao C.-Y.,Yidu Central Hospital of Weifang | Wang X.,Qingzhou Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: An animal model of myocardial infarction is of great significance for the study of the pathogenesis, pathophysiology and evaluation of treatment methods of human coronary artery disease. Many researchers aim to improve survival rate and the success rate of model induction. OBJECTIVE: To improve the method of making a rat model of myocardial infarction. METHODS: One hundred Wistar rats were divided into model group (n=80) and sham-operated group (n=20). The rats were intraperitoneally anesthetized with 3% sodium pentobarbital. Tracheostomy was operated. An automatic breathing apparatus was utilized for assisted breathing. A left anterior thoracotomy was performed at the fourth intercostal space. The left anterior descending coronary artery was ligated. Sham-operated rats underwent similar surgery but without coronary artery ligation. The thoracic cavity was closed layer by layer. The breathing apparatus was removed and tracheal intubation was also removed. To prevent suffocation, the trachea and the neck incision were not closed. RESULTS AND CONCLUSION: Heart morphology and hematoxylin-eosin staining demonstrated that the success rate of establishing myocardial infarction model was 98.6%. The survival rate of model group at 3 weeks after surgery was 88.75%. Totally nine rats died, including seven dead rats during operation and within 24 hours after operation, and two dead rats in later stage. The survival rate of the sham-operated group was 100%. We modified the method of establishing a rat model of myocardial infarction and improved the operation success rate and survival rate of rats, such as anesthesia method, longitudinal incision during tracheotomy, without closing the trachea after operation, and the method of opening and closing the chest.

Zhang L.,Yidu Central Hospital of Weifang | Wang H.,Yidu Central Hospital of Weifang | Tian L.,Yidu Central Hospital of Weifang | Li H.,Women & Childrens Health Care Hospital of Linyi
Tohoku Journal of Experimental Medicine | Year: 2016

Epithelial ovarian cancer (EOC) is one of the worst malignancies in females with poor overall survival due to the rapid metastasis and the absence of ideal biomarkers. Ubiquitin-specific protease 7 (USP7), an important deubiquitinating enzyme, was reported to be upregulated in several cancers, including liver, prostate and colon cancers. Membrane associated RING-CH protein 7 (MARCH7) belongs to the member of the E3 ubiquitin ligases. In addition, MARCH7 regulates T cell proliferation and the neuronal development and participates in the membrane trafficking and protein degradation. Importantly, MARCH7 itself is ubiquitinated and acts as a potential substrate of USP7. However, the roles of USP7 and MARCH7 in EOC remain to be investigated. We collected 121 EOC patients and analyzed the expression levels of USP7 and MARCH7 in tumor tissues with immunohistochemical staining. We found that the high expression of the two proteins was correlated with lymph node metastasis in EOC patients. Univariate and multivariate analyses revealed that the patients with high expression of the two proteins showed poorer prognosis compared with other patients. Subsequently, using SKOV3 human ovarian adenocarcinoma cells, we showed that either USP7 or MARCH7 enhanced the proliferation and invasion abilities. Moreover, USP7 could regulate the expression levels of E-cadherin and β-catenin through the MARCH7 signaling pathway. Our findings indicate that USP7 and MARCH7 are involved in the progression of EOC. In conclusion, analyzing the expression of USP7 and MARCH7 has high prognostic value in predicting EOC prognosis. © 2016 Tohoku University Medical Press.

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