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Zhang W.,Shandong University | Wei L.,Qianfoshan Hospital | Li G.,Shandong University | Sun J.,Yantai Hospital of Binzhou Medical College | And 15 more authors.
International Surgery | Year: 2015

This study aimed to describe the technique details of rapid pore cranial drilling with external ventricular drainage and document its clinical outcomes by highlighting the advantages over the traditional and modified cranial drilling technique. Intraventricular hemorrhage is one of the most severe subtypes of hemorrhagic stroke with high mortality. The amount of blood in the ventricles is associated with severity of outcomes, and fast removal of the blood clot is the key to a good prognosis. Between 1977 and 2013, 3773 patients admitted for intraventricular hemorrhage underwent rapid pore cranial drilling drainage. The therapeutic effects and clinical outcomes were retrospectively analyzed. Of these patients, 1049 (27.8%) experienced complete remission, 1788 (47.4%) had improved condition, and 936 (24.8%) died. A total of 3229 (85.6%) patients gained immediate remission. One typical case was illustrated to demonstrate the efficacy of the rapid pore drilling technique. Rapid pore cranial drilling drainage in patients with intraventricular hemorrhage is fast, effective, and provides immediate relief in patients with severe conditions. It could be a better alternative to the conventional drilling approach for treatment of intraventricular hemorrhage. A randomized controlled trial for direct comparison between the rapid pore cranial drilling drainage and conventional drilling technique is in urgent need. Source

Yan X.-S.,Shandong Research Academy of Traditional Chinese Medicine | Sun D.-D.,Shandong Research Academy of Traditional Chinese Medicine | Guo Y.-W.,Weifang Yidu Central Hospital | Xu X.-G.,Shandong Research Academy of Traditional Chinese Medicine | Yu B.-B.,Shandong Research Academy of Traditional Chinese Medicine
Chinese Traditional and Herbal Drugs | Year: 2016

Objective: To improve the dissolution rate of total triterpenoids from Sclerotii Poriae Cortex. Central composite design response surface methodology was used to optimize formulation of liquid-solid compressed tablets. Methods: The types and ratio of excipients were determined by preliminary test and single factor experiments. Central composite design response surface methodology was used in the optimization of formulation, with dissolution rate as the index. Liquisolid compacts powders, crude drugs, and excipients were characterized by differential scanning calorimetry (DSC). Results: The best prescription was as follow: Liquid ratio was 1:1.67; R value was 18.25; Disintegrating agent was 8%; The ratio of PVPPXL-10 and CMSNa was 1.27 and the tablets hardness was 40-50 N. DSC showed that the characteristic peaks of drug in liquisolid tablets had vanished, and suggested that drugs might be present in liquid-solid compressed tablets as amorphous substance. Conclusion: The formulation of liquid-solid compressed tablet is reasonable. Liquisolid compacts can increase the dissolution rate of total triterpenoids from Sclerotii Poriae Cortex, and suggest that drugs may be present in liquid-solid compressed tablets as amorphous substance. © 2016, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved. Source

Chen H.,Weifang Yidu Central Hospital | Li L.,Qingzhou Peoples Hospital | Xia H.,Qingzhou Peoples Hospital
International Journal of Clinical and Experimental Medicine | Year: 2015

Ketamine is a commonly used short-acting anesthetic and recently attempted to treat pain which is a complication of diabetes. In this study we investigated the effect of ketamine on glucose levels of normal rats and diabetic rats. The results showed that no significance between the glucose levels in ketamine treatment group and saline treatment group at all time points was observed in normal rats. Ketamine did not produce hyperglycemia in normal fasted rats. However, ketamine dose dependently elevated glucose in diabetic rats from 80 mg/kg to 120 mg/kg at 1 hour after injection. The glucose did not return to the levels before treatment in streptozotocin (STZ) induced diabetic rats. Insulin revealed a powerful potency in decreasing glucose levels in diabetic rats. Ketamine did not induce acute hyperglycemia any more after diabetic rats pretreated with insulin. Serum corticosterone was significantly increased in all treatment groups including saline group after 1 hour treatment compared with baseline values. Then the corticosterone declined in both saline treatment groups. However, ketamine induced a more significant increase in corticosterone at 1 hour after injection compared with that of saline control group of diabetic rats. And no decline trend of corticosterone was observed after ketamine treatment 2 hours. Insulin did not reduce the elevated corticosterone level induced by ketamine either. The results suggested that the diabetic rats had a risk of hyperglycaemia when they were treated with ketamine. Pretreatment with insulin is a good symptomatic treatment for hyperglycaemia induced by ketamine. © 2015, E-Century Publishing Corporation. All rights reserved. Source

Yang Y.-C.,Weifang Yidu Central Hospital | Li Y.,Weifang Yidu Central Hospital | Guo M.-T.,Shouguang Peoples Hospital | Zhao J.-M.,Jining No. 1 Peoples Hospital
International Journal of Clinical and Experimental Pathology | Year: 2016

Present study aimed to investigate the effect of cantharidin on reduction in cell viability and induction of apoptosis in KOSC-2 oral cancer cells. Cantharidin treatment for 48 h caused a concentration dependent reduction in KOSC-2 cell viability. The IC50 of cantharidin against KOSC-2 cells was found to be 50 nM after 48 h. KOSC-2 cells showed reduction in size, protrusion of membrane and condensation of nuclear material on treatment with 50 nM concentration of cantharidin for 48 h. Flow cytometry using propydium iodide staining revealed significant (P<0.05) increase in the population of cells in G0/G1 phase of cell cycle in cantharidin treated compared to the control cells. Results from western blot analysis showed increase in the expression of pro-apoptotic BAD and decrease in the expression of anti-apoptotic protein Bcl-2 on treatment with cantharidin in KOSC-2 cells. Cantharidin treatment for 48 h also resulted in a significant increase in the expression of Apaf-1 and AIF as well as translocation of cytochrome c into the cytosol. The activation of caspase-3 in KOSC-2 cells was enhanced significant (P<0.05) in cantharidin treated compared to the control cells. Thus cantharidin treatment inhibits KOSC-2 cell viability and induces apoptosis through mitochondrial pathway. Therefore, cantharidin can be used for the treatment of oral cancer. Source

Chen L.,Peking University | Wu Y.,Weifang Yidu Central Hospital | Yu J.,Peking University | Jiao Z.,Weifang Yidu Central Hospital | And 4 more authors.
Knee Surgery, Sports Traumatology, Arthroscopy | Year: 2011

The increased use of allograft tissue in the reconstruction of anterior cruciate ligament has brought more focus to the effect of storage and treatment on allograft. The purpose of this study was to observe the effect of histology and biomechanics on Achilles tendon in rabbits through repeated freezing-thawing before allograft tendon transplantation. Rabbit Achilles tendons were harvested and processed according to the manufacture's protocol of tissue bank, and freezing-thawing was repeated three times (group 1) and ten times (group 2). Those received only one cycle were used as controls. Then, tendons in each group were selected randomly to make for histological observations and biomechanics test. Histological observation showed that the following changes happened as the number of freezing-thawing increased: the arrangement of tendon bundles and collagen fibrils became disordered until ruptured, cells disrupted and apparent gaps appeared between tendon bundle because the formation of ice crystals. There were significant differences between the experimental and control groups in the values of maximum load, energy of maximum load and maximum stress, whereas no significant differences existed in other values such as stiffness, maximum strain, elastic modulus, and energy density. Therefore, repeated freezing-thawing had histological and biomechanical effect on Achilles tendon in rabbits before allograft tendon transplantation. This indicates that cautions should be taken in the repeated freezing-thawing preparation of allograft tendons in clinical application. © 2010 Springer-Verlag. Source

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