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Wang S.,Weifang Peoples Hospital | Rui R.,Weifang Yidu Central Hospital | Zhang A.,Weifang Peoples Hospital | Liu X.,Weifang Peoples Hospital | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2017

Acute myocardial infarction (AMI) has a high incidence and causes severe consequences. Induced pluripotent stem (iPS) cells derives from patient’s own cells and has become one research focus for treating cardiovascular disease. The role and function of iPS cells on myocardial fibrosis after AMI, however, has not been illustrated. Healthy male Wistar rats were randomly divided into control, sham and AMI group. The AMI model was generated by occlusion of left anterior coronary artery. iPS cell transplantation group received mouse derived iPS cells after AMI model. M-type ultrasound was used to evaluate cardiac function of all rats. Immunohistochemistry staining was employed to evaluate the change of myocardial fibrosis. ELISA was adopted for measuring type I collagen content. Real time PCR and Western blotting were employed to quantify mRNA and protein levels of Bax or Bcl-2 in myocardial tissues, respectively. Left ventricular end-stage systolic dimeter (LVEDS), leaf ventricular end-stage diabolic diameter (LVEDD) and left ventricular mass index (LVMI) were all significantly increased in AMI model rats, which also showed elevated myocardial fibrosis, type I collagen content and Bax expression, along with decreased Bcl-2 mRNA or protein level (P<0.05 compared to sham group). iPS cell transplantation led to lower LVESD, LVEED and LVMI levels accompanied with fewer myocardial fibrosis, type I collage, Bax expression plus increased Bcl-2 expression (P<0.05 compared to AMI group). iPS cells can improve the condition of cardiac infarction and fibrosis via modulating apoptosis balance, protecting myocardial cells and suppressing type I collagen proliferation. © 2017, E-Century Publishing Corporation. All rights reserved.


Shi Y.-F.,Shanghai JiaoTong University | Ju W.-L.,Weifang Yidu Central Hospital | Zhu Y.-P.,Shanghai JiaoTong University | Xia S.-J.,Shanghai JiaoTong University | Sun X.-W.,Shanghai JiaoTong University
Urolithiasis | Year: 2017

Ureteric stenting is an effective drainage method in patients with acute urinary tract infection caused by ureteral calculi; however, the optimal ureteral stent indwelling time has not been clearly defined. The aim of this study was to evaluate the effect of ureteric stent indwelling time on the treatment of acute infection secondary to urinary tract calculi. A total of 142 patients with acute infection caused by urinary tract calculi were identified retrospectively from January 2011 to August 2015 at our institution. 63 patients were with ureteric stenting for 7 days (A group) and 79 patients with ureteric stenting for more than 7 days (B group). The patient characteristics of two groups were analyzed and the clinical data before and after stenting were compared. The postoperative complication outcomes were collected and analyzed. Effective drainage obtained from ureteral stenting clearly abated the infection after stenting for 7 days; WBC count, WBCs in urine, and positive rate of urine culture were significantly decreased compared with the condition of immediate stenting. Both groups showed similar stone clearance rates (96.8% vs. 96.2%, p = 0.841), and there was no significant difference in the rate of postoperative complications, especially related to urinary tract infection (6.3% vs. 6.3%, p = 1.000). It is safe and effective for patients with acute urinary tract infection secondary to urinary tract calculi to be treated by ureteroscopic lithotripsy after stenting for one week. Prolonging the stenting period achieves no added benefit for patients. © 2017 Springer-Verlag Berlin Heidelberg


Chen L.,Peking University | Wu Y.,Weifang Yidu Central Hospital | Yu J.,Peking University | Jiao Z.,Weifang Yidu Central Hospital | And 4 more authors.
Knee Surgery, Sports Traumatology, Arthroscopy | Year: 2011

The increased use of allograft tissue in the reconstruction of anterior cruciate ligament has brought more focus to the effect of storage and treatment on allograft. The purpose of this study was to observe the effect of histology and biomechanics on Achilles tendon in rabbits through repeated freezing-thawing before allograft tendon transplantation. Rabbit Achilles tendons were harvested and processed according to the manufacture's protocol of tissue bank, and freezing-thawing was repeated three times (group 1) and ten times (group 2). Those received only one cycle were used as controls. Then, tendons in each group were selected randomly to make for histological observations and biomechanics test. Histological observation showed that the following changes happened as the number of freezing-thawing increased: the arrangement of tendon bundles and collagen fibrils became disordered until ruptured, cells disrupted and apparent gaps appeared between tendon bundle because the formation of ice crystals. There were significant differences between the experimental and control groups in the values of maximum load, energy of maximum load and maximum stress, whereas no significant differences existed in other values such as stiffness, maximum strain, elastic modulus, and energy density. Therefore, repeated freezing-thawing had histological and biomechanical effect on Achilles tendon in rabbits before allograft tendon transplantation. This indicates that cautions should be taken in the repeated freezing-thawing preparation of allograft tendons in clinical application. © 2010 Springer-Verlag.


Yang Y.-C.,Weifang Yidu Central Hospital | Li Y.,Weifang Yidu Central Hospital | Guo M.-T.,Shouguang Peoples Hospital | Zhao J.-M.,Jining No 1 Peoples Hospital
International Journal of Clinical and Experimental Pathology | Year: 2016

Present study aimed to investigate the effect of cantharidin on reduction in cell viability and induction of apoptosis in KOSC-2 oral cancer cells. Cantharidin treatment for 48 h caused a concentration dependent reduction in KOSC-2 cell viability. The IC50 of cantharidin against KOSC-2 cells was found to be 50 nM after 48 h. KOSC-2 cells showed reduction in size, protrusion of membrane and condensation of nuclear material on treatment with 50 nM concentration of cantharidin for 48 h. Flow cytometry using propydium iodide staining revealed significant (P<0.05) increase in the population of cells in G0/G1 phase of cell cycle in cantharidin treated compared to the control cells. Results from western blot analysis showed increase in the expression of pro-apoptotic BAD and decrease in the expression of anti-apoptotic protein Bcl-2 on treatment with cantharidin in KOSC-2 cells. Cantharidin treatment for 48 h also resulted in a significant increase in the expression of Apaf-1 and AIF as well as translocation of cytochrome c into the cytosol. The activation of caspase-3 in KOSC-2 cells was enhanced significant (P<0.05) in cantharidin treated compared to the control cells. Thus cantharidin treatment inhibits KOSC-2 cell viability and induces apoptosis through mitochondrial pathway. Therefore, cantharidin can be used for the treatment of oral cancer.


Yan X.-S.,Shandong Research Academy of Traditional Chinese Medicine | Sun D.-D.,Shandong Research Academy of Traditional Chinese Medicine | Guo Y.-W.,Weifang Yidu Central Hospital | Xu X.-G.,Shandong Research Academy of Traditional Chinese Medicine | Yu B.-B.,Shandong Research Academy of Traditional Chinese Medicine
Chinese Traditional and Herbal Drugs | Year: 2016

Objective: To improve the dissolution rate of total triterpenoids from Sclerotii Poriae Cortex. Central composite design response surface methodology was used to optimize formulation of liquid-solid compressed tablets. Methods: The types and ratio of excipients were determined by preliminary test and single factor experiments. Central composite design response surface methodology was used in the optimization of formulation, with dissolution rate as the index. Liquisolid compacts powders, crude drugs, and excipients were characterized by differential scanning calorimetry (DSC). Results: The best prescription was as follow: Liquid ratio was 1:1.67; R value was 18.25; Disintegrating agent was 8%; The ratio of PVPPXL-10 and CMSNa was 1.27 and the tablets hardness was 40-50 N. DSC showed that the characteristic peaks of drug in liquisolid tablets had vanished, and suggested that drugs might be present in liquid-solid compressed tablets as amorphous substance. Conclusion: The formulation of liquid-solid compressed tablet is reasonable. Liquisolid compacts can increase the dissolution rate of total triterpenoids from Sclerotii Poriae Cortex, and suggest that drugs may be present in liquid-solid compressed tablets as amorphous substance. © 2016, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


Chen H.,Weifang Yidu Central Hospital | Li L.,Qingzhou Peoples Hospital | Xia H.,Qingzhou Peoples Hospital
International Journal of Clinical and Experimental Medicine | Year: 2015

Ketamine is a commonly used short-acting anesthetic and recently attempted to treat pain which is a complication of diabetes. In this study we investigated the effect of ketamine on glucose levels of normal rats and diabetic rats. The results showed that no significance between the glucose levels in ketamine treatment group and saline treatment group at all time points was observed in normal rats. Ketamine did not produce hyperglycemia in normal fasted rats. However, ketamine dose dependently elevated glucose in diabetic rats from 80 mg/kg to 120 mg/kg at 1 hour after injection. The glucose did not return to the levels before treatment in streptozotocin (STZ) induced diabetic rats. Insulin revealed a powerful potency in decreasing glucose levels in diabetic rats. Ketamine did not induce acute hyperglycemia any more after diabetic rats pretreated with insulin. Serum corticosterone was significantly increased in all treatment groups including saline group after 1 hour treatment compared with baseline values. Then the corticosterone declined in both saline treatment groups. However, ketamine induced a more significant increase in corticosterone at 1 hour after injection compared with that of saline control group of diabetic rats. And no decline trend of corticosterone was observed after ketamine treatment 2 hours. Insulin did not reduce the elevated corticosterone level induced by ketamine either. The results suggested that the diabetic rats had a risk of hyperglycaemia when they were treated with ketamine. Pretreatment with insulin is a good symptomatic treatment for hyperglycaemia induced by ketamine. © 2015, E-Century Publishing Corporation. All rights reserved.


Zhang G.-F.,Weifang Yidu Central Hospital | Li C.-X.,Weifang Yidu Central Hospital | Liu Z.-Q.,Weifang Yidu Central Hospital | Ma S.,Weifang Yidu Central Hospital | Chen H.-B.,Weifang Yidu Central Hospital
European Review for Medical and Pharmacological Sciences | Year: 2016

The varied therapeutic approache like radiotherapy, chemotherapy, surgery, etc primarily aimed to target cancer cells specifically Despite these efforts, they are not completel successful in eliminating this deadly pathologica state. These failures ultimately lead to cance reoccurrence, which is again, another burnin problem associated with cancer. The prime reaso for the above observation has been found t be the development of resistance by cancer cell towards cancer drugs or cancer-initiating cell (cancer stem cells) remain unaffected by existin treatment procedures. Recent research ha evolved two drugs, salinomycin and apoptin that hold great potential for the future of cance treatment not only for restricting malignancy, bu also in preventing tumor recurrence The present review article will put light on thes new upcoming cancer stem cell targeting agents.


PubMed | Weifang Yidu Central Hospital
Type: Journal Article | Journal: European review for medical and pharmacological sciences | Year: 2016

The varied therapeutic approaches like radiotherapy, chemotherapy, surgery, etc. primarily aimed to target cancer cells specifically. Despite these efforts, they are not completely successful in eliminating this deadly pathological state. These failures ultimately lead to cancer reoccurrence, which is again, another burning problem associated with cancer. The prime reason for the above observation has been found to be the development of resistance by cancer cells towards cancer drugs or cancer-initiating cells (cancer stem cells) remain unaffected by existing treatment procedures. Recent research has evolved two drugs, salinomycin and apoptin, that hold great potential for the future of cancer treatment not only for restricting malignancy, but also in preventing tumor recurrence. The present review article will put light on these new upcoming cancer stem cell targeting agents.


PubMed | Weifang Yidu Central Hospital
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2016

BACKGROUND This study aimed to analyze the relationship of UGT2B7 and UGT1A4 polymorphisms with metabolism of valproic acid (VPA) and lamotrigine (LTG) in epileptic children. MATERIAL AND METHODS We administered VPA (102) and LTG (102) to 204 children with epilepsy. Blood samples were collected before the morning dose. Serum concentration of LTG was measured by high-performance liquid chromatography (HPLC). Serum VPA concentration was tested by fluorescence polarization immunoassay. UGT2B7 A268G, C802T, and G211T polymorphisms, as well as UGT1A4 L48V polymorphism, were assayed by direct automated DNA sequencing after PCR. Evaluation of efficacy was conducted using the Engel method. RESULTS The adjusted serum concentration of VPA was 4.26 g/mL per mg/kg and LTG was 1.56 g/mL per mg/kg. Multiple linear regression analysis revealed that VPA or LTG adjusted concentration showed a good linear relation with sex and age. UGT2B7 A268G and C802T polymorphisms were demonstrated to affect the serum concentration of VPA (F=3.147, P=0.047; F=22.754, P=0.000). UGT1A4 L48V polymorphism was not related with the serum concentration of LTG (F=5.328, P=0.006). In the efficacy analysis, we found that C802T polymorphism exerted strong effects on efficacy of VPA (=9.265, P=0.010). L48V polymorphism also showed effects on efficacy of LTG (=17.397, P=0.001). CONCLUSIONS UGT2B7, UGT1A4 polymorphisms play crucial roles in metabolism of VPA and LTG.


PubMed | Weifang Yidu Central Hospital
Type: | Journal: Hormones & cancer | Year: 2016

The microRNAs (miRNAs) have been suggested as a tumor suppressor in recent years. miR-15b was reported to exert an anti-oncogenic role in the proliferation, migration, and invasion of diverse tumor cells. However, the mechanisms underlying miR-15b-mediated biology of glioblastoma are still unclear. In the present study, the expression of miR-15b was down-regulated in glioblastoma tumor tissues and U87 and U251 cells, but insulin-like growth factor receptor 1 (IGF1R) expression became up-regulated in these tumor tissues and cells (all p<0.001). Furthermore, IGF1R expression was inversely associated with miR-15b expression. Notably, patients with lower miR-15b expression have a much shorter survival period compared with high expression (log-rank test p=0.045). In vitro data demonstrated that miR-15b mimics inhibited the proliferation, cell cycle arrest, and invasion of U87 and U251 cells. Besides, we validated IGF1R as a direct target of miR-15b using dual luciferase assays, and IGF1R plasmids partially abrogated miR-15b mimics inhibited cell proliferation. In vivo, miR-15b mimics indeed repressed cell proliferation in mouse xenograft model. In conclusion, our study demonstrated that miR-15b inhibits the progression of glioblastoma cells through targeting IGF1R, and miR-15b can be recommended as a tumor suppressor in the progression of glioblastoma.

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