Deng Y.-Q.,Renmin University of China |
Yang Y.-Q.,Renmin University of China |
Wang S.-B.,Yichang Yiling Hospital |
Li F.,Renmin University of China |
And 3 more authors.
PLoS ONE | Year: 2015
Mast cell (MC) degranulation is the foundation of the acute phase of allergic rhinitis (AR). Previously, down regulation of GATA binding protein 3 (GATA-3) was shown to suppress MC activation in an AR mouse model. Binding of microRNA-135a (miR-135a) to GATA-3 was also observed, and overexpression of this miRNA decreased GATA-3 mRNA and protein expression. However, the effects of miR-135a on MCs during AR are currently unknown. In the present study, we utilized a lentiviral (LV) vector to intranasally administer miR-135a to ovalbumin (OVA)-sensitized AR mice. Following miR-135a treatment, the total serum IgE concentration observed during AR was significantly reduced. In the nasal mucosa, the expression of T-box expressed in T cells (T-bet) was higher, whereas that of GATA-3 was lower in the AR mice following miRNA treatment. Notably, during AR, the ratio of type 1 T-helper cells (Th1) to type 2 (Th2) cells in the spleen is unbalanced, favoring Th2. However, administering miR-135a to the AR mice appeared to balance this ratio by increasing and decreasing the percentage of Th1 and Th2 cells, respectively. MiR-135a also appeared to strongly suppress the infiltration of eosinophils and MCs into the nasal mucosa, and it was specifically localized in the MCs, suggesting that its influence is modulated through regulation of GATA-3 in these cells. Additional work identifying the full therapeutic potential of miR-135a in the treatment of AR and diseases involving allergen-induced inflammation is warranted. © 2015 Deng et al.
Zhang Q.-M.,China Three Gorges University |
Peng Y.,China Three Gorges University |
You H.,China Three Gorges University |
Li S.-H.,Yichang Yiling Hospital |
And 6 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: There are a few reports addressing the application of mild hypothermia to the study of repairing the nerve injury, but few reports have addressed the effects of mild hypothermia on the migration of neural stem cells in the brain following transplantation. Objective: To explore the effects of mild hypothermia on the migration of bone marrow mesenchymal stem cells transplanted into the lateral ventricle of rats with cerebral ischemia, as well as the effect on the infarct volume. Methods: A focal cerebral ischemic injury model of right middle cerebral artery occlusion was established using modified Longa's method in Sprague-Dawley rats. Fifty rats were divided into mild hypothermia group, control group and sham-operation group. Local mild hypothermia was applied in the mild hypothermia group before transplantation for the treatment of acute cerebral ischemia. Normal body temperature was maintained in control group before transplantation for the treatment of acute cerebral ischemia. The right carotid artery of rats in the sham-operation group was separated and ligated after anesthesia. At 24 hours following model establishment in the mild hypothermia group and the control group, bone marrow mesenchymal stem cells labeled with 5-BrdU were transplanted into the rat lateral ventricle. The amount of BrdU-positive cells in the brain tissue in each group was measured by immunohistochemistry at 5, 14 and 21 days following injection in each group. Results and Conclusion: At 14 days after transplantation, a majority of labeled bone marrow mesenchymal stem cells had migrated to the infarct area. At various time points following transplantation, the number of BrdU-positive cells in the cortex around infarction focus was obviously greater in the hypothermia group compared with the control group (P < 0.05). Compared with the control group, the infarct volume in the mild hypothermia group at various time points was reduced significantly (P < 0.05). Results indicate that mild hypothermia before transplantation may promote the direct migration of bone marrow mesenchymal stem cells, and can reduce the infarct volume.
Zou Y.,Renmin University of China |
Wang Y.,Renmin University of China |
Wang S.-B.,Yichang Yiling Hospital |
Kong Y.-G.,Renmin University of China |
And 3 more authors.
Experimental and Therapeutic Medicine | Year: 2016
Chronic rhinosinusitis (CRS) is a heterogeneous disease, with varying immunological and histopathological features. The CC chemokine ligand 19 (CCL19) can stimulate T cells and antigen‑presenting cells into secondary lymphoid node formation, as observed in allergic rhinitis, inflammatory bowel disease and other inflammatory disorders. The purpose of this study was to investigate the expression and significance of CCL19 in CRS. Samples of uncinate process mucosa or nasal polyps were taken from patients with CRS (without or with nasal polyps) and normal controls during surgery. Hematoxylin and eosin, periodic acid Schiff and Masson trichrome staining were used for analysis of the nasal polyps. Western blot and immunofluorescence were used to detect CCL19 expression in the nasal polyps and normal nasal mucosa tissues. Spearman correlation analysis was used to analyze the association between CCL19 and blood eosinophil counts. The results showed that CCL19 protein levels in CRS (with or without nasal polyps) were significantly upregulated compared with those in controls. CCL19 expression in eosinophilic CRS was significantly higher than in non‑eosinophilic CRS. CCL19 expression in fibroinflammatory and edematous type CRS with nasal polyps was higher than in controls; the upregulation was greater in the edematous type. Immunofluorescence assays showed that CCL19 was mainly expressed by CD68+ macrophages. Spearman correlation analysis demonstrated a positive correlation between CCL19 and blood eosinophils. The upregulation of CCL19 in CRS may play a protective role by limiting eosinophil infiltration and the extent of edema to exert anti‑inflammatory and immunomodulatory effects. © 2016, Spandidos Publications. All rights reserved.
Zhang L.,Wuhan University of Science and Technology |
Gong R.-L.,Wuhan University of Science and Technology |
Han Q.-R.,Yichang Yiling Hospital |
Shi Y.-Q.,Wuhan University of Science and Technology |
And 4 more authors.
Asian Journal of Andrology | Year: 2015
There has been little focus on men's reproductive health (RH) in China. This descriptive study conducted in Yiling District, Yichang, China, surveyed male knowledge of sexual physiology and RH to assess levels of knowledge, attitudes and practices (KAPs) regarding prevention of sexually transmitted diseases (STDs). A total of 3933 men, aged 18-59 years (mean, 40.3 years), were recruited by cluster random sampling. They completed a questionnaire in the presence of an interviewer, with items related to subject characteristics, RH knowledge, and subjective symptoms of the reproductive system. Physical examination and reproductive system disease diagnosis were performed. Participants' occupations were predominantly skilled labor (80.5%). Nearly four-fifths (78.5%) respondents had at least one reproductive disease. Over half of respondents were aware of and declared a positive attitude about sexual physiology and safe sex, and 70% of them selected to visit a doctor when they had a reproductive disorder. However, only 41.9% believed human immunodeficiency virus/acquired immunodeficiency syndrome could be transmitted through breastfeeding, and 64.6% incorrectly thought they could avoid contracting STDs by cleaning their genitals after intercourse. In addition, 45% discriminated against and were unwilling to be friends with infected persons. Nearly 45% of those with a reproductive system disorder refused to discuss it with friends or family members. These results indicate that this cohort of Chinese men had a certain degree of KAP about RH, whereas some aspects require further public health education in the general population. It is necessary to disseminate accurate knowledge of STD risk in China based on sociodemographic characteristics. © 2015 AJA, SIMM & SJTU. All rights reserved.
Du J.-S.,Yichang Yiling Hospital |
Wang G.-Y.,Yichang Yiling Hospital |
Zhong B.,Yichang Yiling Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: A single hemostatic material has been proved not to facilitate wound healing or to produce certain adverse reactions; while composites composed of two or three different materials can improve the advantage and histocompatibility of hemostatic materials. OBJECTIVE: To investigate the effect of gelatin sponge impregnated with hemocoagulase solution on amount of bleeding in patients with lumbar fractures undergoing posterior spinal decompression. METHODS: Fifty patients with lumbar fractures who were scheduled for open reduction, pedicle screw fixation and laminectomy were enrolled, including 25 cases treated with gelatin sponge impregnated with hemocoagulase before surgical incision closure as test group and 25 cases treated with single gelatin sponge before surgical incision closure as control group. Postoperative drainage volume, drainage time, length of stay, number of re-admissions and postoperative complications were compared between the two groups. RESULTS AND CONCLUSION: The postoperative drainage volume, drainage time and length of stay in the test group were significantly less than those in the control group (P < 0.000 1). No infection, epidural hematoma, or re-admission of patients was found, and there was no hemocoagulase-impregnated absorbable gelatin spongerelated adverse reaction. These findings indicate that posterior laminectomy with hemocoagulase-impregnated gelatin sponge can significantly reduce patients’ postoperative wound drainage and shorten the length of stay. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All Rights Reserved.