Jin W.,Yi du Central Hospital |
Dong T.,Yi du Central Hospital |
Luan C.,Yi du Central Hospital |
Zhao C.,Yi du Central Hospital |
Zhang Y.,Yi du Central Hospital
Chinese Journal of Clinical Oncology | Year: 2010
Objective: To investigate the effect of simvastatin on proliferation and nuclear factor kappa B (NFκB) expression in the esophageal cancer (EsC) cell EC9706. Methods: The human esophageal cancer cell line EC9706 was cultured. MTT assay was used to determine the effect of simvastatin on the proliferation of EC9706 cells at different concentrations. Morphological changes of the cells were observed by transmission electron microscopy and laser scanning confocal microscopy. Flow cytometry was used to detect the effect of simvastatin on the apoptotic rates and cell cycle distribution, and immunocytochemistry was conducted to investigate the effect of the drug on expression of NF-κB in the EC9706 cells. Results: It was confirmed by MTT assay that within the concentration interval of 0-20 μmol/L, as the concentration and exposure time were increased, the inhibition ratio of simvastatin on the EsC cell EC9706 gradually increased. It could be seen by transmission electron microscopy and laser scanning confocal microscopy that the typical morphological change of apoptosis occurred in the cells. Flow cytometry was used to detect the apoptotic rate, and the cell cycle was blocked at the phase G0/G1. Cells were exposed to simvastatin at increasing concentrations (0, 5, 10, 20 μmol/L) for 72 h and positive rates of the NF-κB nuclear staining were observed (46.2 ± 5.0, 38.3 ± 4.8b, 30.8 ± 4.0b, 23.4 ± 2.1b %) (a P<0.05, b P<00.01 vs. control group). After exposure to 20 μmol/L of simvastatin for increasing times (0, 24, 48 and 72 h), the positive rates of NF-κB nuclear staining decreased (37.8 ± 2.8, 31.6 ± 2.6a, 25.6 ± 2.2b, 16.8 ± 2.0b) (a P<00.05, b P<00.01 vs. control group). With an increase of the concentration and extension of the time of simvastatin exposure, the NF-κB expression in the EsC cell EC9706 gradually decreased. Conclusion: Simvastatin significantly inhibits the proliferation of EsC cell EC9706 and there is a positive correlation between the concentration and effectiveness of the drug. This mechanism of action might be related to the apoptosis resulting from inhibition of NF-κB expression in the EC9706 cells.