Han Y.,Qingdao University |
Jiang Q.,Qingdao University |
Gao H.,Qingdao University |
Fan J.,Qingdao University |
And 6 more authors.
Cell Biochemistry and Biophysics | Year: 2014
To investigate the molecular mechanisms of polypeptide from Chlamys farreri (PCF)’s anti-apoptotic effect, HaCaT cells were exposed to 20 mJ/CM2 UVB, with or without pretreatment of TGF-β1 antagonist SB431542, inducible nitric oxide synthase (iNOS) inhibitor S-methylisothiourea sulfate (SMT), nitric oxide scavenger carboxy-PTIO, or 1.42, 2.84, and 5.69 mM PCF, or iNOS transfection (without UVB exposure). Apoptosis was confirmed with Hoechst 33258 staining; RT-PCR and western blot were used to determine the expression levels of iNOS and TGF-β1 signaling pathway. Both UVB exposure and iNOS transfection-induced apoptosis in UVB-exposed HaCat cells, while PCF, SB431542, SMT, and carboxy-PTIO all inhibited UVB-induced apoptosis. TGF-β1, Smad4, and Smad7 mRNA levels were all altered, similarly, iNOS, TGF-β1, and pSmad2/3 protein levels were all altered in UVB-exposed HaCaT cells. In pretreated cells, SB431542, SMT, carboxy-PTIO, and 1.42–5.69 mM PCF all inhibited UVB-induced apoptosis. Moreover, PCF treatment inhibited the expression levels of iNOS, TGF-β1, pSmad2/3, and Smad4, while increased the expression level of Smad7. SB431542 did not significantly alter iNOS expression, while SMT and carboxy-PTIO significantly altered TGF-β1 signaling level. The anti-apoptotic effect of PCF in UVB-exposed HaCaT cells involves the inhibition of iNOS expression and subsequently inhibition of TGF-β1 signaling pathway. © 2014, Springer Science+Business Media New York.
Gao Z.,Shandong University of Technology |
Miao X.,CAS Beijing Institute of Genomics |
Miao X.,University of Chinese Academy of Sciences |
Zhang X.,Yellow Sea Fishery Research Institute |
And 11 more authors.
Algal Research | Year: 2016
Astaxanthin in microalga Haematococcus pluvialis has been studied for years, but there is still little known about the metabolic variation of astaxanthin biosynthesis. Thus, iTRAQ-based proteomic data were analysed on H. pluvialis in response to salicylic acid (SA) and jasmonic acid (JA) inductions with a time course. While a subset of 257 proteins was screened in the JA treatment with 119 proteins up-regulated and 138 proteins down-regulated, 272 proteins were in the SA treatment, with 123 of significant up-regulation and 149 of down-regulation. Meanwhile, proteins enriched in lipid metabolism were differentially expressed in both JA and SA treatments over time. This was consistent with the genetic transcriptional expressions involved in the fatty acid biosynthesis. However, the proteins' coding for lipid metabolism was not correlated to the differential expressions of FA biosynthesis genes in either JA or SA inductions. These results provide a new insight on the interrelationship between FA synthesis genes' regulations and FA/astaxanthin biosynthesis, and also highlight the importance of protein post-translational modifications for the astaxanthin accumulation. Furthermore, about 61 differentially expressed proteins were identified as putative transcription factors (TFs) at the translational level and assigned to 24 families. Many different TFs were observed between JA and SA treatments, suggesting different signaling pathways involved in the JA and SA induced H. pluvialis cells. These results also form a fundamental basis to facilitate future research towards genetically bioengineered astaxanthin biosynthesis in H. pluvialis. © 2016 Elsevier B.V.
Gao Z.,Shandong University of Technology |
Gao Z.,Yellow Sea Fishery Research Institute |
Meng C.,Shandong University of Technology |
Meng C.,Yellow Sea Fishery Research Institute |
And 8 more authors.
Enzyme and Microbial Technology | Year: 2012
The green alga Haematococcus pluvialis can produce large amounts of pink carotenoid astaxanthin which is a high value ketocarotenoid. In our study, transcriptional expression patterns of eight carotenoid genes in H. pluvialis in response to SA were measured using qRT-PCR. Results indicated that both 25 and 50. mg/L salicylic acid (SA) could increase astaxanthin productivity and enhance transcriptional expression of eight carotenoid genes in H. pluvialis. But these genes exhibited different expression profiles. Moreover, SA25 (25. mg/L SA) induction had a greater effect on the transcriptional expression of ipi-1, psy, pds, crtR-B and lyc (more than 6-fold up-regulation) than on ipi-2, bkt and crtO, but SA50 (50. mg/L SA) treatment had a greater impact on the transcriptional expression of ipi-1, ipi-2, pds, crtR-B and lyc than on psy, bkt and crtO. Furthermore, astaxanthin biosynthesis under SA was up-regulated mainly by ipi-1, ipi-2, psy, crtR-B, bkt and crtO at transcriptional level, lyc at post-transcriptional level and pds at both levels. Summarily, these results suggest that SA constitute molecular signals in the network of astaxanthin biosynthesis. Induction of astaxanthin accumulation by SA without any other stimuli presents an attractive application potential in astaxanthin production with H. pluvialis. © 2012 Elsevier Inc.