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Naikawadi V.B.,University of Pune | Naikawadi V.B.,Chandmal Tarachand Bora College | Ahire M.L.,University of Pune | Ahire M.L.,Yashwantrao Chavan Institute of Science | And 2 more authors.
Applied Microbiology and Biotechnology

Evolvulus alsinoides L. is used for preparation of ‘Shankhapushpi’, an important popular ayurvedic drug that contributes considerably to the improvement of memory power. The improvement is attributed to the presence of furanocoumarin scopoletin, a metabolite with a wide range of biological activities. This report describes, for the first time, an in vitro culture system for propagation and enhanced production of scopoletin. Different concentrations of auxins and cytokinins individually and in combination were used in Murashige and Skoog (MS) medium to induce shoot regeneration in cotyledonary nodal explants and callus formation in leaf explants. The best response was achieved in MS medium fortified with 5.0 μM 6-benzyladenine (BA) in which 96 % of cultures produced 7.6 ± 0.6 shoots per explant. Regenerated shoots were rooted on MS medium with 5.0 μM indole-3-acetic acid (IAA). Plantlets were successfully acclimatized and established in soil. MS medium fortified with 10 μM BA + 5.0 μM IAA showed maximum growth and accumulation of scopoletin in cell cultures. Cell cultures could be maintained over 24 months. The influences of auxins, cytokinins, organic acids, amino acids, and fungal-derived elicitors on production of scopoletin were studied. Presence of either l-arginine, sodium pyruvate, or yeast extract highly promoted scopoletin production as compared with control and achieved 75.02-, 72.13-, and 57.98-fold higher accumulation, respectively. The results presented herein have laid solid foundation for large-scale production of scopoletin and further investigation of its purification and utilization as a novel pharmaceutical drug. © 2015 Springer-Verlag Berlin Heidelberg Source

Pai S.R.,Shivaji University | Nimbalkar M.S.,Sahyadri Group Enriching Nature | Pawar N.V.,Yashwantrao Chavan Institute of Science | Dixit G.B.,Shivaji University
Industrial Crops and Products

Simple, cost effective, quick and sustainable technique was investigated for determining Betulinic acid (BA) from samples of Ancistrocladus heyneanus. The methods comprised of continuous shaking, Soxhlet, ultra sonication and microwave assisted extraction techniques. RP-HPLC technique was used to quantify BA from varied samples and confirmation of the samples was done using TLC, FT-IR and FT-Raman methods. The study also evaluated productivity of operational parameters such as solvent composition and extraction time for three distinctive parts of the plant (green leaves, brown leaves and stem). Brown leaves showed response to continuous shaking extraction and ultrasonic extraction techniques with 95% Aq. MeOH as a good extraction solvent. BA was detected and quantified in continuous shaking method with 15, 30 and 45. min of exposure time. Comparison of 6. min with 12. min of ultra sonication, showed longer sonication diminished extraction efficiencies. Concluding, brown leaves in 95% MeOH and ultrasonic extraction technique to be the fastest, easiest and best method for detection and screening of BA from A. heyneanus. © 2011 Elsevier B.V. Source

Nimbalkar M.S.,Shivaji University | Nimbalkar M.S.,Sahyadri Group for Enriching Nature | Pai S.R.,Regional Medical Research Center | Pai S.R.,Sahyadri Group for Enriching Nature | And 4 more authors.
Food Chemistry

Reliable and reproducible techniques for identification and quantification of amino acids usually require derivatization. However, techniques such as LC-MS/MS may perhaps sideline the derivatization with significant accuracy. Understanding the interaction between free amino acids and several physiological processes apparently requires that the pool be studied in the seed itself. Data on free amino acid content of grain amaranth is very inadequate. The present analysis revealed a higher concentration of essential amino acids especially Threonine and Tryptophan than non essential amino acids. The study constitutes first report of use of LC-MS/MS method for analysing the free amino acid composition of amaranth grain. © 2012 Elsevier Ltd. All rights reserved. Source

Khogare B.T.,Raje Ramrao Mahavidyalaya | Khogare B.T.,Yashwantrao Chavan Institute of Science | Anuse M.A.,Shivaji University | Piste P.B.,Yashwantrao Chavan Institute of Science | Kokare B.N.,Raje Ramrao Mahavidyalaya
Desalination and Water Treatment

A newly reported chelating agent, 4-heptylaminopyridine has been used for selective extraction and separation of palladium(II) from salicylate media at pH 0.5 is studied. The effect of different parameters such as pH, weak organic acid concentration, extractant concentration, equilibrium time, diluents, and stripping agent has been evaluated. Successful stripping of palladium(II) from the loaded organic phase is achieved with 6.0 M ammonia (2 × 10 mL) solution. The method affords separation of palladium(II) from binary and ternary mixtures and is applicable to the analysis of synthetic mixtures, alloys, catalysts, and water samples. The method is simple, selective, cost effective, and reproducible. © 2015 Balaban Desalination Publications. All rights reserved. Source

Patil V.S.,Lal Bahadur Shastri College | Patil S.V.,Yashwantrao Chavan Institute of Science | Deshmukh H.V.,Yashwantrao Chavan Institute of Science | Pathade G.R.,Fergusson College
Nature Environment and Pollution Technology

Soil salinity is a major problem in Maharashtra. Attempt is made to isolate salt-tolerant, thermotolerant, nitrogen fixing, phosphate solubilising Azotobacter spp. from the saline soil of Khodashi village in Satara district. Eight Azotobacter spp. were isolated from the saline soils. They were confirmed based on morphological, cultural and biochemical characteristics. They were tested for saline and thermal tolerance. The phosphate solubilizing potential of the these Azotobacter isolates was qualitatively evaluated by the formation of halos (clear zones) around the colonies growing on solid medium containing tribasic calcium phosphate as a sole phosphorus source. The results showed that phosphate solubilising, salt tolerant and thermotolerant Azotobacter spp. could be a promising source for the development of saline-Alkali soil-based agriculture. Source

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