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Kuz'mina V.V.,Russian Academy of Sciences | Shalygin M.V.,Russian Academy of Sciences | Skvortsova E.G.,Yaroslavl State Agricultural Academy
Journal of Evolutionary Biochemistry and Physiology | Year: 2012

Effect of temperature on proteinases activities of enteral microbiota and of intestinal mucosa was studied in five fish species (roach Rutilus rutilus, crucian carp Carassius carassius, common perch Perca fluviatilis, pike-perch Zander lucioperca, and pike Esox lucius) belonging by the nutrition type to different ecological groups. Essential differences of temperature characteristics of proteinases of intestinal mucosa and of enteral microbiota are revealed in fish belonging to different ecological groups. The character of the t-function of proteinases of intestinal mucosa and enteral microbiota for casein and hemoglobin as a rule is different. The values of the apparent Eact proteinases of intestinal mucosa for casein in most cases are higher than those of enteral microbiota, while those for hemoglobin, on the contrary, are lower. The lowest values of relative proteinase activities for casein in the zone of low temperatures (38 and 45.3% of the maximal activity) and the Eact value (less than 2.0 kcal/mol) are found at study of proteinases of enteral microbiota in common perch and crucian carp. The latter indicates a significant adaptability of the enteral microbiota proteinases of common perch and crucian carp to functioning at low temperatures. © 2012 Pleiades Publishing, Ltd. Source


Flerova E.A.,Yaroslavl State Agricultural Academy | Balabanova L.V.,Russian Academy of Sciences
Journal of Evolutionary Biochemistry and Physiology | Year: 2013

Analysis of data on ultrastructure of granulocytes of freshwater and marine bony fish of orders Salmoniformes, Cypriniformes, and Perciformes showed that in all studied species there were revealed two types of granulocytes-neutrophils and eosinophils. The exception was the bluefish Pomatomus saltatrix L. whose pronephros hemopoietic tissue was found to contain one type of the granulocyte line-neutrophils. The identification parameters of granular leukocytes are specific granules filling the cytoplasm. The main form of specific granules in neutrophils of bony fish of various phylogenetic groups is an elongated granule with different distribution of fibrils or a granule that has fibrils-formed crystalloid. Eosinophil granules are mainly of large, electron-dense, homogenous form. © 2013 Pleiades Publishing, Ltd. Source


Kuz'mina V.V.,Russian Academy of Sciences | Skvortsova E.G.,Yaroslavl State Agricultural Academy | Shalygin M.V.,Russian Academy of Sciences
Inland Water Biology | Year: 2015

The effect of temperature on some characteristics of proteinases functioning in the tissues of food items of fish (juvenile sterlet Acipenser ruthenus L., Black Azov Sea tyulka Clupeonella cultriventris (Nordm.), perch Perca fluviatilis L. and fish of fam. Cyprinidae) was studied for the first time. The differences in the shapes of curves describing the temperature dependence of prey-tissue enzymes hydrolyzing casein and hemoglobin were revealed. As a rule, the values of the energy of activation of caseinolytic proteinases of prey tissues are higher than those of hemoglobinolytic proteinases. The lowest values of this parameter are the characteristic of tissue hemoglobinolytic proteinases of perch and cyprinids. This suggests better adaptability of these fish tissues for functioning at low temperatures. Revealed differences show that thermal characteristics of tissue proteinases of potential food items of fish depend on species phylogenetic specificity. © 2015, Pleiades Publishing, Ltd. Source


Flerova E.A.,Yaroslavl State Agricultural Academy
Russian Journal of Marine Biology | Year: 2016

The ultrastructure of cells that form mesonephros tissues in eight species of bony fish of the Black Sea, Gaidropsarus mediterraneus (L.), Lisa aurata (Risso), Trachurus mediterraneus (Staindachner), Diplodus annularis (L.), Spicara flexuosa (Rafinesque), Gobius niger jozo L., Mullus barbatus ponticus (Essipov) and Scorpaena porcus (L.), was studied. It was shown that the ultrastructure of agranulocytes and rodlet cells in the studied species is similar to that of cells in freshwater bony fish. The differences were observed in the number of ionocyte mitochondria, in the vesicle ultrastructure in the cells with radial vesicle array, and in the ultrastructure of the specific secondary granules of neutrophils and eosinophils. © Pleiades Publishing, Ltd., 2016. Source


Kuz'mina V.V.,Russian Academy of Sciences | Shalygin M.V.,Yaroslavl State Agricultural Academy | Skvortsova E.G.,Yaroslavl State Agricultural Academy
Inland Water Biology | Year: 2012

The effect of temperature on the chyme proteinase activities of the perch Perca fluviatilis L. and the roach Rutilus rutilus (L.) was investigated. The two fish species showed different characters of temperature dependence and different values of activation energy for caseinolytic and hemoglobinolytic chyme proteinases. The perch was characterized by a higher level of relative proteinase activities and lower activation energy for protein hydrolysis at a temperature of 0-10°C than the roach. © 2012 Pleiades Publishing, Ltd. Source

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