Chen X.-F.,Yantaishan Hospital |
Liu Y.,Xiangyang Central Hospital
Biomedicine and Pharmacotherapy | Year: 2016
Growing evidence suggests that microRNA plays an essential role in the development and metastasis of many tumor progressions, including cervical cancer. Aberrant miR-744 expression has been indicated in many growth of tumor, the mechanism of miR-744 inhibits both the proliferation and metastatic ability for cervical cancer remains unclear. Accumulating evidences reported that Bcl-2 signal pathway plays an important role in the cellular process, such as apoptosis, cell growth and proliferation. The goal of this study was to identify miR-744 that could inhibit the growth, migration, invasion, proliferation and metastasis of gastric cancer through targeting Bcl-2 expression. Real-time PCR (RT-qPCR) was used to quantify miR-744 expression in vitro and vivo experiments. The biological functions of miR-744 were determined via cell proliferation. Our study indicated that miR-744 targeted on Bcl-2, which leads to the inactivation of apoptosis signaling and the cell proliferation of cervical cancer cells, ameliorating cervical cancer growth and progression. In addition, both up-regulation of miR-744 and down-regulation of Bcl-2 could stimulate Caspase-3 expression, promoting apoptosis of cervical cancer cells. Therefore, our research revealed the mechanistic links between miR-744 and Bcl-2 in the pathogenesis of cervical cancer through modulation of Caspase-3, leading to the inhibition of cervical cancer cell growth. And targeting miR-744 could be served as a novel strategy for future cervical cancer therapy clinically. © 2016 Elsevier Masson SAS.
Gao J.,Yantaishan Hospital |
Lv C.,Yantaishan Hospital
Latin American Journal of Pharmacy | Year: 2014
Both tiliroside and noscapine are promising drugs for treating hypopharyngeal cancer, and high possibility exists for the co-administration of these two drugs. The present study aims to analyze the mechanism for the interaction between tiliroside and CYP3A4 using molecular docking method, and then predict the drug-drug interaction potential between tiliroside and noscapine. Protein data bank was used to search the crystal structure of CYP3A4, and molecular structure of tiliroside was drawn using Chem- Draw software. The missing residues were added using the protein preparation wizard in the Schrodinger suite of programs. The binding molecule ketoconazole was firstly extracted form the binding site of CYP3A4 before the docking of tiliroside into the activity cavity of CYP3A4. The results showed that strong hydrogen interaction existed between this compound and amino Glu374 and Arg372, and the distance between tiliroside catalytic site and active center is 2.08 Å, indicating the good interaction between tiliroside and CYP3A4. Co-docking using tiliroside and noscapine showed the new drug-drug interaction between tiliroside and noscapine. In conclusion, the interaction between tiliroside and cytochrome P450 (CYP) 3A4 was elucidated using molecular docking and new drug-drug interaction between tiliroside and noscapine was also indicated in the present study. © 2014, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.
Kong M.,Yantaishan Hospital |
Ba M.,Yantai University
NeuroReport | Year: 2012
Accumulated amyloid-β (Aβ) is a well-known cause of neuronal apoptosis in Alzheimer's disease and exerts its action partly by inducing mitochondrial dysfunction. Previous studies have suggested a neuroprotective role for mitochondrial ATP-sensitive potassium (K ATP) channel openers against Aβ damages, but the molecular details were unclear. Recent evidence indicates that endoplasmic reticulum (ER) stress also plays an important role in the process of cell apoptosis. It remains to be determined whether K ATP channel openers mediate their potential neuroprotective role by inhibiting ER stress pathways. The mRNA and protein expression levels of caspase-12, an ER-specific caspase, were observed. Here we showed that in response to the treatment with Aβ 25-35 (10 μM) for 24 h the mRNA and protein expression levels of caspase-12 were significantly upregulated; however, this change could be partly reversed by pretreatment with diazoxide (1 mM) for 1 h. This effect was negated by 5-hydroxydecanoate, a selective mitochondrial K ATP channel blocker. Our results indicate that the cytoprotective efficacy of diazoxide under Aβ 25-35-induced insults is mediated, at least in part, by inhibition of ER stress. Demonstration of the neuroprotective action of diazoxide provides additional insights into the pathogenic mechanisms of Aβ 25-35 toxicity and defines possible molecular targets for therapeutic intervention. © 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Zhao Y.,Yantaishan Hospital
Zhonghua wai ke za zhi [Chinese journal of surgery] | Year: 2016
OBJECTIVE: To investigate the influences of the distal femoral cut, the anterior and posterior femoral cuts, the proximal tibial cut on the dynamic alignment of the lower extremity in total knee arthroplasty.METHODS: Based on the three-dimensional geometric model, imitating the flexion movement of the knee without axial rotation after total knee arthroplasty, the influence of each of the three bone cuts on the dynamic alignment was analyzed with the assumption of standard bone cuts of the other two and equality of the medial and lateral soft tissue balancing. The dynamic alignment was defined as the angle between the mechanical axis of the tibia and the sagittal plane of the body when the knee was in any angle of flexion. With two of the three major bone cuts standard, the track of the tibial movement was established when the other one bone cut deviated from the ideal section of angle A. Based on the principle of geometry, the mathematical formula were established to present the influences of three bone cuts on the dynamic alignment of the lower extremity.RESULTS: All of the three kinds of bone cuts in total knee arthroplasty influenced the dynamic alignment of the lower extremity not just in one static position, but during the whole range of motion. At the θ angle of knee flexion, the alignment of the lower extremity was arcsin(cosθsinA) when the varus/valgus femoral component alignment was A; the alignment of the lower extremity was arcsin(sinθsinA) when the rotational femoral component alignment was A; the alignment of the lower extremity was A when the varus/valgus tibial component alignment was A.CONCLUSION: The influences of the distal femoral cut, the anterior and posterior femoral cuts, the proximal tibial cut on the dynamic alignment of the lower extremity in total knee arthroplasty are dynamically changed during the flexion movement of the knee.
Li B.,Yantai University |
Yu D.,Yantaishan Hospital |
Xu Z.,Yantaishan Hospital
Journal of Molecular Neuroscience | Year: 2013
Abstracts: Parkinson's disease (PD), which is estimated to affect approximately 1 % of the population over the age of 65, is the second most common neurodegenerative disorder after Alzheimer's disease. It was reported that pathogenic mutations in DJ-1 lead to autosomal recessive early-onset familial Parkinsonism. The L166P mutant of DJ-1 is the most commonly studied loss-of-function mutation in early onset familial PD, but the underlying mechanisms are still unknown. Edaravone is a powerful free radical scavenger used in clinical treatment for cerebral ischemic stroke. In the present study, we investigated the effects of edaravone on the neurotoxicity in PD-induced isoforms of DJ-1 containing the mutation L166P. Our results indicated that edaravone was able to significantly attenuate oxidative stress and improve mitochondrial function. Furthermore, edaravone was found to reduce apoptosis in Neuro2a cells through modulation of mitochondria-dependent apoptosis pathways. Interestingly, our result also demonstrated that edaravone was able to up-regulate VMAT2 expression in N2a cells in a dose-dependent manner. Our findings enhance the understanding of the neuro-protective effects of edaravone in cell models and suggest that edaravone offers significant protection in a PD-related in vitro model. © 2013 Springer Science+Business Media New York.
Cui Y.,Yantaishan Hospital |
Chen J.,Shanghai JiaoTong University |
He Z.,Nantong University |
Xiao Y.,Shanghai JiaoTong University
Cellular Physiology and Biochemistry | Year: 2013
Background/Aims: SUZ12 and EZH2 are two main components of polycomb repressive complex 2 (PRC2) that is known to be of great importance in tumorigenesis. EZH2 has been reported to play a vital role in pathogenesis of human cancer. However, whether SUZ12 has equivalent roles in tumorigenesis has not been demonstrated. Here, we investigated a possible role of SUZ12 for the proliferation of gastric cancer cells. Methods: Western-blot analysis was used to detected the levels of SUZ12, H3K27me3, EZH2 and p27 in ten gastric cell lines. SUZ12 was depleted by RNA interference. Cell cycle was detected by flow cytometry. Luciferase assays was to analyze whether miR-200b directly regulate SUZ12. Results: We found that SUZ12 depletion mediated by RNA interference (RNAi) led to a reduction of gastric cell numbers and arrested the cell cycle at G1/S point. As an important G1/S phase inhibitory gene, p27 is re-induced to some extent by SUZ12 knockdown. Furthermore, we demonstrated that SUZ12 was directly downregulated by miR-200b. Conclusion: We provide evidence suggesting that SUZ12 may be a potential therapeutic target for gastric cancer. Copyright © 2013 S. Karger AG, Basel.
Bi H.Y.,Yantaishan Hospital
Zhonghua wai ke za zhi [Chinese journal of surgery] | Year: 2013
To study the clinical effect of arthroscopic anterior cruciate ligament (ACL) construction with different transplants. From March 2006 to April 2009, 86 patients including 60 male and 26 female undergoing arthroscopic ACL reconstruction were prospectively randomized consecutively into autograft group (44 patients, using autogeneic hamstring tendons) and allograft group (42 patients, using allogenic lower extremity tendons). The age of those patients were 22 - 56 years, averaging (32 ± 7) years. The operations were made by the same doctor with the standard technology. The postoperative effects were assessed by the range of motion and tibia forward distance, Lachman test, pivot shift test, Daniel test, IKDC scores systems, Lysholm-Tegner scores. Seventy-nine patients were followed up, 41 patients in autograft groups averaged 39.6 months and 38 patients in allograft group averaged 37.4 months. The operation time of autograft group was (87 ± 11) minutes, that of allograft group was (55 ± 10) minutes (t = 15.732, P < 0.05). The time of postoperative fever of autograft group was (3.2 ± 1.4) days, that of allograft groups was (7.6 ± 5.3) days (t = 5.740, P < 0.05). The Lysholm scores of autograft group was 42 ± 7 before operation, and 89 ± 8 at final follow-up. The Lysholm scores of allograft group was 44 ± 6 before operation, and 87 ± 9 at final follow-up. There was statistic difference in both groups between before operation and final follow-up (t = 13.534 and 17.768, P < 0.05).But no statistic difference existed between the two groups (P > 0.05). The Tegner scores of autograft group was 2.9 ± 2.1 before operation, and 7.7 ± 1.2 at final follow-up. The Tegner scores of allograft group was 2.7 ± 1.4 before operation, and 7.1 ± 1.6 at final follow-up. There was statistic difference in both groups between before operation and final follow-up (t = 16.004 and 12.338, P < 0.05).No statistic difference existed between the two groups (P > 0.05). The KT2000 results showed that the anterior displacement of autograft groups was (10.7 ± 3.5) mm before operation and (5.0 ± 2.7) mm at final follow-up, the anterior displacement of allograft groups was (10.9 ± 2.9) mm before operation and (6.5 ± 2.4) mm at final follow-up, there was statistic difference between before and after operation in anterior displacement in two groups (t = 16.354 and 13.296 P < 0.05). There was no difference between two groups before operation and at final follow-up. Compared to before operation, the IKDC scores were improved greatly after operation (P < 0.05). The clinical effect of arthroscopic ACL construction with allograft transplants is near to autograft.
Li B.,Qingdao University |
Yu D.,Yantaishan Hospital |
Xu Z.,Yantaishan Hospital
Brain Research | Year: 2014
Inhibition of Aβ production and clearance of senile plaques have been considered as potential strategies in the treatment of Alzheimer's disease (AD). Activated protein C (APC) is an important factor in the anticoagulant system. However, whether APC can influence the condition of a chronic neurodegenerative process, such as that present in AD, is unknown. In this study, we found that administration of APC on AD Tg2576 mice significantly reduced amyloid β production and helped to facilitate cognitive improvement. APC could also reduce levels of Aβ40 and Aβ42 produced in APPswe cells, an AD cell model. Further results demonstrated that APC did not change the levels of Aβ-degrading enzymes, insulin-degrading enzyme (IDE), or neprilysin (NEP). Next, we found that APC promoted sAPPα and CTFα release and inhibited sAPPβ and CTFβ release, thereby indicating that APC could regulate Aβ secretion by shifting APP processing from the amyloidogenic pathway toward the nonamyloidogenic pathway. Correspondingly, further study revealed that ADAM-10 expression was increased by APC, suggesting that APC inhibits Aβ secretion through stimulating activity of α-secretase. These findings support the idea that APC could hold therapeutic potential in the treatment of AD. © 2013 Elsevier B.V.
Wang S.,Yantaishan Hospital |
Zhao Y.,Yantaishan Hospital
Journal of Arthroplasty | Year: 2013
We explored if there was an increased risk of DVT in patients with diabetes mellitus undergoing total knee arthroplasty within 14. days followup. We reviewed 245 patients undergoing total knee arthroplasty in our hospital between 2003 and 2011. The incidence of DVT within 14. days after operations was compared between diabetic patients and non-diabetic patients. There were 37 patients with DVT in the diabetes group and 88 in the non-diabetes group within 14. days followup (p. =0.002). The risk of DVT in patients with diabetes mellitus was 2.76 times the risk in patients without diabetes mellitus using logistic regression modeling (OR. =2.76, p. =0.003). Although it is still controversial, patients with diabetes had a higher incidence rate of DVT after total knee arthroplasty in our study. © 2013 Elsevier Inc..
Li Q.,Yantaishan Hospital
American Journal of Therapeutics | Year: 2016
To determine the effects of 80-mg atorvastatin administration for the first time in patients with acute ST segment elevation myocardial infarction (STEMI) before emergency percutaneous coronary intervention (PCI). A total of 118 patients with STEMI who underwent emergency PCI were enrolled in this study. The patients were divided into 80-mg group (n = 59) and 40-mg group (n = 59), according to the loading dose of atorvastatin firstly before operation. The occurrence of no-reflows and changes of HbA1c were observed preoperatively and postoperatively on second and fifth days. All patients were followed up for 1 year with major adverse cardiac events (MACE) recorded. The incidence of no-reflow in 80-mg group was obviously lower than in 40-mg group (13.56% vs. 25.42%) (χ = 4.374, P = 4.374). The preoperative HbA1c levels exhibited no significant difference between 80-mg group and 40-mg group (P > 0.05). The postoperative HbA1c levels in 2 groups showed a trend of gradual decline, which were lower in 80-mg group than in 40-mg group for second day, fifth day, first month, sixth month, and 12th month (all P < 0.05). The postoperative incidence of MACE in 80-mg group was significantly lower than in 40-mg group for sixth and 12th months (both P < 0.05). The incidence of MACE in patients with reflow in 80-mg and 40-mg groups was significantly higher than in patients with no-reflow who were in 80-mg and 40-mg groups for postoperative 12th month (both P < 0.05). The first loading high dose of atorvastatin can significantly prevent occurrence of postoperative no-reflow in patients with STEMI after PCI, reduce HbA1c levels and the incidence of MACE. Clinical randomized controlled trial with larger sample size is required to confirm this finding. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.