Yuhuangding, China
Yuhuangding, China

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Cao B.,CAS Institute of Microbiology | Huang G.-H.,Chinese National Institute for Viral Disease Control and Prevention | Huang G.-H.,Xinjiang Medical University | Pu Z.-H.,YanTai Yu Huang Ding Hospital | And 16 more authors.
Chest | Year: 2014

Background: Since 2008, severe cases of emerging human adenovirus (HAdV) type 55 (HAdV-55) were reported sporadically in China. But no comparative studies had been conducted to discern the differences in epidemiologic and clinical abnormalities between HAdV-55 and other types (HAdV-7, HAdV-3, HAdV-14, HAdV-50, and HAdV-C). Methods: A multicenter surveillance study for adult and adolescent community-acquired pneumonia (CAP) was conducted prospectively in Beijing and Yan Tai between November 2010 and April 2012. A standardized data form was used to record clinical information. The viral DNA extracted from the clinical samples or adenovirus viral isolates was sequenced. Results: Among 969 cases, 48 (5%) were identified as adenovirus pneumonia. Six branches were clustered: HAdV-55 in 21, HAdV-7 in 11, HAdV-3 in nine, HAdV-14 in four, HAdV-50 in two, and HAdV-C in one. Most HAdV-55 cases were identified during February and March. All the hypervariable regions of the hexon genes of the 21 HAdV-55 strains were completely identical. Patients who had HAdV-55 were about 10 years older (P 5 .027) and had higher pneumonia severity index scores (P 5 .030) compared with those with other types (HAdV-7, HAdV-3, HAdV-14, HAdV-50, and HAdV-C). Systemic BP was also higher among patients in the HAdV-55 group (P 5 .006). Unilateral or bilateral consolidations were the most common radiologic findings in both patients with HAdV-55 and those with other types (57.9% vs 36%). More than one-half of the patients were admitted to hospital; oxygen therapy was given to 29.2% of the 48 patients, and two needed mechanical ventilation. Conclusions: HAdV-55 has established itself as a major pneumonia pathogen in the Chinese population, and further surveillance and monitoring of this agent as a cause of CAP is warranted. © 2014 American College of Chest Physicians.


Lopes T.,Hospital St Maria | Sangam K.,Vedanayagam Hospital and Postgraduate Institute | Alken P.,University of Mannheim | Barroilhet B.S.,Hospital San Borja Arriaran | And 3 more authors.
Journal of Endourology | Year: 2011

Purpose: The study focused on the use of balloon or telescopic/serial dilation methods in percutaneous nephrolithotomy (PCNL) in the Global PCNL Study. Patients and Methods: Centers worldwide provided data from consecutive patients who were treated with PCNL during a 1-year period. Tract dilation was performed using a balloon or telescopic/serial dilator. Patient characteristics, perioperative complications, and treatment outcomes were assessed by the treating physician. Postoperative complications were graded according to the modified Clavien grading system. Results: A total of 5537 eligible patients were entered in the database from November 2007 to December 2009, including 2277 (41.1%) who received balloon dilation and 3260 (58.9%) who received telescopic/serial dilation. The predominant method used was telescopic/serial dilation in Asia (94.7%) and South America (98.0%), and balloon dilation in North America (82.6%). In Europe, the rates of balloon (50.7%) and telescopic/serial (49.3%) dilation procedures were similar. The rates of bleeding (9.4% vs 6.7%), blood transfusions (7.0% vs 4.9%), and drop in mean hematocrit level (4.5% vs 2.5%) were higher in the balloon vs telescopic/serial dilator group. Clavien scores II and IIIA were slightly in favor of the telescopic/serial dilator group. Median operative time was longer in the balloon dilation group (94.0 min vs 60.0 min). Conclusions: The Global PCNL Study has identified differences in the method of dilation used between centers in Asia, Europe, and the United States. In the balloon dilation group, a total longer operative time and higher bleeding and transfusion rates were observed. The differences in outcome may be influenced by patient heterogeneity, including previous anticoagulation therapy or surgical procedures, in addition to the number of stones treated and rate of staghorn calculi, which were all higher in the balloon group. © 2011, Mary Ann Liebert, Inc. 2011.


PubMed | Yantai Yu Huang Ding Hospital, Peoples Hospital of Rizhao and Peoples Hospital of Weifang
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2017

BACKGROUND Slug has been found to promote migration and invasion of many cancer cells, including anaplastic thyroid cancer (ATC). Thus, targeting Slug expression could provide new approaches for the treatment of patients with ATC. MATERIAL AND METHODS Small interfering RNA (siRNA) targeting Slug (Slug siRNA) was used to construct clonal derivatives in the metastatic ATC SW1736 cells. Slug cDNA transfection was used to restore the Slug expression in the Slug siRNA-transfected SW1736 cells (Slug siRNA/SW1736). E-cadherin siRNA was used to inhibit E-cadherin expression in the Slug siRNA/SW1736 cells. The SW1736 cell migration, invasion, and signaling pathway was analyzed in vitro. Furthermore, the stable Slug siRNA-transfected SW1736 clones were used for the lung metastasis assay in an in vivo mouse model. RESULTS Targeting Slug expression in SW1736 cells showed a 45% decrease in migration and an 85% decrease in invasiveness in vitro. Knockdown of E-cadherin by E-cadherin siRNA transfection or Slug overexpression by Slug cDNA transfection restored the invasive and migrative ability in SW1736 cells. In addition, we found an 80% decrease in the number of macroscopic lung metastases nodes of mice by in vivo analysis. Western blot assay showed that Slug expression was inhibited and E-cadherin expression was increased in the Slug siRNA-transfected tumors. CONCLUSIONS Targeting Slug signaling pathway is effective in preventing lung metastasis in ATC.


Qu J.-X.,Capital Medical University | Gu L.,Capital Medical University | Pu Z.-H.,YanTai Yu Huang Ding Hospital | Yu X.-M.,Capital Medical University | And 5 more authors.
BMC Infectious Diseases | Year: 2015

Better knowledge of distribution of respiratory viruses (RVs) in adolescents and adults with community-acquired pneumonia (CAP) is needed. Methods: To investigate the RVs etiology among adolescents and adults with CAP, according to age and pneumonia severity index (PSI), a multi-center, prospective study was conducted from November 2010 to April 2012. Fifteen RVs were tested by polymerase chain reaction (PCR). Bacteria were detected by urinary antigen, conventional culture and PCR. Results: Mean (SD) age and median (IQR) PSI score of 954 patients enrolled was 45.2 (19.5) years (range 14-94) and 42 (36). RVs were found in 262 patients (27.5%): influenza virus A (IFV A, 9.9%) comprised of pandemic H1N1 (6.7%) and seasonal H3N2 (3.5%), human rhinovirus (4.3%), adenovirus (4.2%), human metapneumovirus (1.8%), parainfluenza virus 1, 3 and 2 (1.7%, 1.5% and 1.2%). Influenza virus B, enterovirus, respiratory syncytial virus, human coronavirus and parainfluenza virus 4 were rarely detected (<1%). Frequency of IFV A was highest among patients aged between 45-64 years (p < 0.001), while adenovirus among patients aged 14-17 years (p < 0.001), no differences was found in other RVs. The proportion of pandemic H1N1 increased with severity of pneumonia evaluated by PSI (P < 0.05). Conclusions: The proportion of RVs in CAP is higher than previously reported. IFV A pneumonia are usually found in patients older than 45 years, while, adenovirus pneumonia are common in adolescents and young adults. Pandemic H1N1 virus is still recognized by PSI as a high-severity pathogen. The findings contribute baseline data on viral CAP study in China. © Qu et al.


Fan H.,Yantai University | Qi D.,Yantai Yu Huang Ding Hospital | Yang M.,Shandong Target Drug Research Co. | Fang H.,Yantai University | And 3 more authors.
Phytomedicine | Year: 2013

In the present study, we evaluated the anti-inflammatory effect of 4-methoxy-5- hydroxycanthin-6-one (CAN), a natural alkaloid isolated from Picrasma quassioides. CAN significantly inhibited the production of NO and the release of TNF-α induced by LPS in macrophage RAW 264.7. Western blot showed that CAN can downregulate the expression of iNOS protein. After oral administration, CAN (3, 9, and 27 mg/kg) reduced the development of carrageenan-induced paw edema and complete Freund's adjuvant (CFA)-induced chronic arthritis in rats. The observed results indicated that pre-treatment with CAN might be an effective therapeutic intervention against inflammatory diseases including chronic arthritis. © 2012 Elsevier GmbH.


Yu Z.H.,Yantai Yu Huang Ding Hospital
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2010

AIM: To investigate the expression of TLR4 and NF-kappaB in ulcerative colitis (UC). METHODS: Colonic biopsy specimens were collected from active UC and controls. The expression of TLR4 and NF-kappaBp 65 were analyzed by immunohistochemistry (IHC) and RT-PCR. RESULTS: RT-PCR revealed a significant increase of TLR4 and NF-kappaBp 65 antigen expression in colonic mucosa of UC compared with colonic mucosa of controls (TLR4: 143.658+/-33.870, 30.531+/-8.442, t=24.253, P<0.01; NF-kappaBp65: 185.773+/-37.625, 23.810+/-7.038, t=31.664, P<0.01). IHC show that the expression of TLR4 and NF-kappaB was significantly higher in colonic mucosa of UC compared with colonic mucosa of controls. CONCLUSION: The expression of the TLR4 and NF-kappaB was increased in the colonic mucosa of UC compared with colonic mucosa of controls. It may be closely associated with the course of ulcerative colitis.


Huang L.-Y.,Yantai Yu Huang Ding Hospital | Cui J.,Yantai Yu Huang Ding Hospital | Lin S.-J.,Yantai Yu Huang Ding Hospital | Zhang B.,Yantai Yu Huang Ding Hospital | Wu C.-R.,Yantai Yu Huang Ding Hospital
World Journal of Gastroenterology | Year: 2014

AIM: To evaluate the efficacy, safety and feasibility of endoscopic full-thickness resection (EFR) for the treatment of gastric submucosal tumors (SMTs) arising from the muscularis propria. METHODS: A total of 35 gastric SMTs arising from the muscularis propria layer were resected by EFR between January 2010 and September 2013. EFR consists of five major steps: injecting normal saline into the submucosa; pre-cutting the mucosal and submucosal layers around the lesion; making a circumferential incision as deep as the muscularis propria around the lesion using endoscopic submucosal dissection and an incision into the serosal layer around the lesion with a Hook knife; a full-thickness resection of the tumor, including the serosal layer with a Hook or IT knife; and closing the gastric wall with metallic clips. RESULTS: Of the 35 gastric SMTs, 14 were located at the fundus, and 21 at the corpus. EFR removed all of the SMTs successfully, and the complete resection rate was 100%. The mean operation time was 90 min (60-155 min), the mean hospitalization time was 6.0 d (4-10 d), and the mean tumor size was 2.8 cm (2.0-4.5 cm). Pathological examination confirmed the presence of gastric stromal tumors in 25 patients, leiomyomas in 7 and gastric autonomous nerve tumors in 2. No gastric bleeding, peritonitis or abdominal abscess occurred after EFR. Postoperative contrast roentgenography on the third day detected no contrast extravasation into the abdominal cavity. The mean follow-up period was 6 mo, with no lesion residue or recurrence noted. CONCLUSION: EFR is efficacious, safe and minimally invasive for patients with gastric SMTs arising from the muscularis propria layer. This technique is able to resect deep gastric lesions while providing precise pathological information about the lesion. With the development of EFR, the indications of endoscopic resection might be extended. © 2014 Baishideng Publishing Group Inc. All rights reserved.


Liu J.,Yantai Yu Huang Ding Hospital | Chi N.,Yantai Yu Huang Ding Hospital | Chen H.,Yantai Yu Huang Ding Hospital | Zhang J.,Yantai Yu Huang Ding Hospital | And 3 more authors.
Brain Research | Year: 2013

Neurotoxicity of amyloid β (Aβ) plays an important role in Alzheimer's disease (AD) pathogenesis. In this study, we researched the potential protective effects of resistin against Aβ neurotoxicity in mouse Neuro2a (N2a) cells transfected with the Swedish amyloid precursor protein (Sw-APP) mutant and Presenilin exon 9 deletion mutant (N2a/D9), which overproduced Aβ with abnormal intracellular Aβ accumulation. The results show increased levels of ROS, NO, protein carbonyls, and 4HNE in N2a/D9 cells, which were attenuated by resistin treatment in a dose dependent manner. We also found that resistin could improve mitochondrial function in N2a/D9 cells through increasing the level of ATP and mitochondrial membrane potential. MTT and LDH assay indicated that N2a/D9 cells show increased vulnerability to H 2O2-induced insult, which could be ameliorated by resistin. Mechanically, we found that resistin prevented apoptosis signals through reducing the ratio of Bax/Bcl2, the level of cleaved caspase-3, and attenuating cytochrome C release. Finally, the results demonstrated that resistin did not change the production of Aβ1-40 and Aβ1-42 in N2a/D9 cells, which suggests that the protective effects of resistin are independent of APP metabolism. This raises the possibility of novel AD therapies using resistin. © 2013 Elsevier B.V.


Huang L.-Y.,Yantai Yu Huang Ding Hospital | Cui J.,Yantai Yu Huang Ding Hospital | Liu Y.-X.,Yantai Yu Huang Ding Hospital | Wu C.-R.,Yantai Yu Huang Ding Hospital | Yi D.-L.,Yantai Yu Huang Ding Hospital
World Journal of Gastroenterology | Year: 2012

AIM: To explore endoscopic therapy methods for gastric stromal tumors originating from the muscularis propria. METHODS: For 69 cases diagnosed as gastric stromal tumors originating from the muscularis propria, three types of endoscopic therapy were selected, based on the size of the tumor. These methods included endo-scopic ligation and resection (ELR), endoscopic submu-cosal excavation (ESE) and endoscopic full-thickness resection (EFR). The wound surface and the perforation of the gastric wall were closed with metal clips. Immunohistostaining for CD34, CD117, Dog-1, S-100 and smooth muscle actin (SMA) was performed on the resected tumors. RESULTS: A total of 38 cases in which the tumor size was less than 1.2 cm were treated with ELR; three cases were complicated by perforation, and the perforations were closed with metal clips. Additionally, 18 cases in which the tumor size was more than 1.5 cm were treated with ESE, and no perforation occurred. Finally, 13 cases in which the tumor size was more than 2.0 cm were treated with EFR; all of the cases were complicated by artificial perforation, and all of the perforations were closed with metal clips. All of the 69 cases recovered with medical treatment, and none required surgical operation. Immunohistostaining demonstrated that among all of the 69 gastric stromal tumors diagnosed by gastroscopy, 12 cases were gastric leiomyomas (SMA-positive), and the other 57 cases were gastric stromal tumors. CONCLUSION: Gastric stromal tumors originating from the muscularis propria can be treated successfully with endoscopic techniques, which could replace certain surgical operations and should be considered for further application. © 2012 Baishideng. All rights reserved.


Liu J.,Yantai Yu Huang Ding Hospital | Rong C.T.,Fuyang Peoples Hospital | Li Y.,Yantai University | Liu X.X.,Yantai Yu Huang Ding Hospital | And 2 more authors.
Journal of Urology | Year: 2014

Purpose We investigated oxidative damage caused by and antioxidant responses to peroxiredoxins in the mouse testis at different time points after vasectomy. Materials and Methods Mice were divided into a sham operated control group and a vasectomized group. Testicular samples were collected 2 to 120 days postoperatively. Histology and cell apoptosis were evaluated by hematoxylin and eosin staining, and TUNEL assay, respectively. Oxidative damage was determined using a commercial malondialdehyde kit. The expression of peroxiredoxin and other antioxidant enzyme mRNAs was detected by reverse transcriptase-polymerase chain reaction. Western blot and immunohistochemistry were performed to examine peroxiredoxin protein expression. Results Histology revealed severe damage in vasectomized testes with high malondialdehyde levels. The alteration in malondialdehyde was in parallel with the process of histological injury and apoptosis. Germ cell apoptosis developed in a time dependent and cell specific manner. The expression of peroxiredoxin 1, 2 and 3, especially peroxiredoxin 2, was significantly up-regulated at the mRNA and protein levels in the testis after vasectomy, which subsequently ameliorated testicular damage toward the control level. In addition, other related antioxidant enzymes, such as Cat and Sod3, showed decreased expression at the mRNA level in vasectomized testes. Conclusions This study shows that vasectomy induces oxidative stress in the mouse testis in the short-term early period after surgery, resulting in histological injury and germ cell apoptosis. Peroxiredoxins may have important roles as antioxidant defenses in vasectomized testis and provide new prevention and therapy alternatives for testicular damage after vasectomy. © 2014 by American Urological Association Educaton and Research, Inc.

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