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Tian X.-H.,Marine Fisheries Research Institute of Shandong Province | Gong X.-H.,Marine Fisheries Research Institute of Shandong Province | Xu Y.-J.,Marine Fisheries Research Institute of Shandong Province | Ren C.-B.,Yantai Shanshui Seafood Co. | And 4 more authors.
Modern Food Science and Technology | Year: 2013

Accumulation of Prometryn in Apostichopus japonicus under laboratory conditions was studied using ultra performance liquid chromatogram tandem mass spectrometry. Apostichopus japonicus was divided randomly, and then exposed to water containing 1.00 μg/L, 10.00 μg/L and 200.00 μg/L Prometryn at 15.0±1.0°C. After exposure, apostichopus japonicus was placed into clean water for the subsequent elimination experiment. The accumulation amount increased with the increase of Prometryn concentration, presenting significantly positive correlation. The highest concentrations (Cmax) of enriched Prometryn for the three concentrations were 4.70 μg/kg, 43.80 μg/kg and 674.00 μg/kg when placed for 1 d, 3 d and 4 d, and the biggest bioconcentration factors (BCF) were 4.70, 4.38 and 3.37. BCF decreased with the increase of the Prometryn concentration, presenting significantly inverse correlation. When Apostichopus japonicus was bred in clean water, Prometryn accumulated was eliminated rapidly. Prometryn was no detected at 2 d. Dtermination performed for 14 times from 7 h to 46 d, showed 23.70±3.07 μg/kg of Prometryn in Apostichopus japonicus. The accumulation and elimination curves of Prometryn in Apostichopus japonicus provided useful information for decontamination of aquatic organisms that have Prometryn contamination. Source


Ren C.-B.,Marine Fisheries Research Institute of Shandong Province | Ren C.-B.,Yantai Shanshui Seafood Co. | Tian X.-H.,Marine Fisheries Research Institute of Shandong Province | Tian X.-H.,Shandong Province Key Laboratory of Restoration for Marine Ecology | And 10 more authors.
Journal of Chinese Mass Spectrometry Society | Year: 2013

The triazine herbicides in seawater were determined by solid phase extraction ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry(UPLC-ESI-MS/MS). UPLC and MS/MS analytical conditions were examined and optimized critically by a series of experiments. Samples were filtrated and purified on the HLB solid phase extraction cartridges. The elution was collected and evaporated to less than 1 mL at 40°C by nitrogen blow, then added to 1 mL acetonitrile and water (1:1, V/V) and ultrasonic wave extraction for 1 min. After filtrated with 0.22 μm filter membrane, the residue was separated by Acquity™ UPLC HSS C18 column (2.1 mm×100 mm×1.8 μm) using gradient elution separation. The mobile phase was a mixture of A (acetonitrile) and B (5 mmol/L ammonium acetate containing 0.1% formic acid) in a flow rate of 0.25 mL/min. Finally analytes were confirmed and quantified using MS/MS system in multiple reaction mode with triple quadrapole analyzer using positive polarity mode. The analytes show good linearity in the range of 1 ng/L to 50 ng/L with correlation coefficient from 0.992 to 0.999. The detection limit is 1 ng/L, and quantification limit is 2 ng/L. The average recoveries at three dose levels(2 ng/L, 5 ng/L and 10 ng/L) are 65.8%-103% with relative standard deviations from 4.84% to 15.2%. In addition, the method has merits of simplicity, sensitivity and rapidity, and can be used for simultaneous determination of triazine herbicides in seawater. Source


Ren C.-B.,Shandong Marine Resource and Environment Research Institute | Ren C.-B.,Yantai Shanshui Seafood Co. | Tian X.-H.,Shandong Marine Resource and Environment Research Institute | Sun Y.,Shandong Marine Resource and Environment Research Institute | And 8 more authors.
Modern Food Science and Technology | Year: 2014

A method for the determination of triazine herbicide residues in Apostichopus japonicus by ultra performance liquid chromatography tandem mass spectrometry was established. In order to investigate the situation of 13 triazine herbicide residues, food safety indexes and risk coefficient were used to evaluate the potential risk of triazine herbicide. The triazine herbicides of atrazine, prometryn and ametryn were detected in 57 samples. The highest detection rate was prometryn (77.2%), followed by atrazine (15.8%) and ametryn (8.77%). The maximum concentration of atrazine, prometryn and ametryn was 0.84 μg/kg, 54.4 μg/kg and 0.69 μg/kg, respectively. The detection ratio in August were significantly higher than those in September, October and November, which suggested that during the collected month, triazine herbicide had few influence on Apostichopus japonicus. According to the assessment of food safety indexes of 57 Apostichopus japonicus samples collected from six cities, the safety indexes of 13 analytes were less than 1, indicating Apostichopus japonicus were in safe and good condition. The risk coefficients of atrazine, prometryn and ametryn were 0.2, 0.38 and 0.2, all less than 1.5, which proved that the three analytes were at low risk group. Therefore, Apostichopus japonicus from six cities was at low risk on triazine herbicides and safe to eat. Source


Xu Y.-J.,Shandong Marine Resource and Environment Research Institute | Sun Y.,Shandong Marine Resource and Environment Research Institute | Ren C.-B.,Yantai Shanshui Seafood Co. | Xue J.-L.,Yantai Shanshui Seafood Co. | And 4 more authors.
Modern Food Science and Technology | Year: 2014

Diethylstilbestrol and its metabolite dienestrol in Ctenopharynodon idellus by oral administration were investigated in their tissue distribution and pharmacokinetics, and they were quantified by UPLC-MS. Diethylstilbestrol concentrations-versus-time was well described by a two-department open model with first-order absorption. For example, after single oral of 1.0 mg/kg (b.w.) diethylstilbestrol, the highest concentration of it was detected in liver, followed by muscle and plasma. The average elimination speed was 12.02 μg/(L·h), 1.33 μg/(kg·h) and 3.59 μg/(kg·h), respectively, for plasma, muscle and liver. No diethylstilbestrol was detected after oral administration of 10 h, 192 h and 168 h. The variation of dienestrol concentrations in plasma, muscle and liver of Ctenopharynodon idellus was similar to that of diethylstilbestrol, which was increased first and then decreased. Concentrations of dienestrol in plasma, muscle and liver reached the peak after oral administration of 1 h, 6 h and 6 h, and no detection after 8 h, 168 h and 144 h. The relevant pharmacokinetic parameters of diethylstilbestrol were calculated by using DAS 2.0. Taking the concentration of 1.0 mg/kg b.w. diethylstilbestrol as example, it was indicated that area under concentration-time curve (AUC) differed greatly and the tissues had different accumulation capability. The overall clearance rate was 1.434 L/(h·kg), 0.126 L/(h·kg) and 0.099 L/(h·kg), indicating diethylstilbestrol was widely distributed and fast eliminated in tissue. It was recommended the eliminated period should be 6 d, 8 d and 10 d. The concentrations versus time curve of diethylstilbestrol provided useful information for studying decontamination of Ctenopharynodon idellus that was contaminated by diethylstilbestrol. Source


Zhang H.-W.,Shandong Marine Resource and Environment Research Institute | Zhang H.-W.,Yantai Shanshui Seafood Co. | Liu H.-H.,Shandong Marine Resource and Environment Research Institute | Liu H.-H.,Shandong Province Key Laboratory of Restoration for Marine Ecology | And 12 more authors.
Journal of Chinese Mass Spectrometry Society | Year: 2015

A method for determination of 9 triazine herbicides residues in aquatic products by gel permeation chromatography-solid phase extraction-gas chromatography tandem mass spectrometry(GPC-SPE-GC-MS/MS) was established. The sample was extracted with ethyl acetate, then cleaned up by gel permeation chromatography and purified by carbon and NH2 solid phase extraction column. The analytes were determined in selected ion monitoring and quantified by external method. Propazine, atrazine, terbuthylazine, simazine and cyprazine show good linearity in the range of 1.0-50.0 μg/L, and the detection limits of the five analytes are 1.0 μg/kg. The average recoveries range from 86.3% to 119% when spiked at 1.0-10.0 μg/kg with relative standard deviations from 5.11% to 10.1%. Simetryn, prometryn, ametryn and terbutryn show good linearity in the range of 2.0-100.0 μg/L, and the detection limits are 2.0 μg/kg. The average recoveries range from 87.6% to 112% when spiked at 2.0-20.0 μg/kg with relative standard deviations from 5.27% to 9.56%. The method is stable and reasonable, and which can be used for the determination of 9 triazine herbicides residues in aquatic products. ©, 2015, Chinese Society for Mass Spectrometry. All right reserved. Source

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