Yantai Academy of Agricultural science

Yantai, China

Yantai Academy of Agricultural science

Yantai, China
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Wang Y.,CAS Institute of Genetics and Developmental Biology | Xin Q.,Yantai Academy of Agricultural science
Crop Journal | Year: 2014

Cytokinin oxidase/dehydrogenase (CKX; EC. regulates cytokinin (CK) level in plants and plays an essential role in CK regulatory processes. CKX proteins are encoded by a small gene family with a varying number of members in different plants. In spite of their physiological importance, systematic analyses of SiCKX genes in foxtail millet have not yet been examined. In this paper, we report the genome wide isolation and characterization of SiCKXs using bioinformatic methods. A total of 11 members of the family were identified in the foxtail millet genome. SiCKX genes were distributed in seven chromosomes (chromosome 1, 3, 4, 5, 6, 7, and 11). The coding sequences of all the SiCKX genes were disrupted by introns, with numbers varying from one to four. These genes expanded in the genome mainly due to segmental duplication events. Multiple alignment and motif display results showed that all SiCKX proteins share FAD- and CK-binding domains. Putative cis-elements involved in Ca2 +-response, abiotic stress response, light and circadian rhythm regulation, disease resistance and seed development were present in the promoters of SiCKX genes. Expression data mining suggested that SiCKX genes have diverse expression patterns. Real-time PCR analysis indicated that all 11 SiCKX genes were up-regulated in embryos under 6-BA treatment, and some were NaCl or PEG inducible. Collectively, these results provide molecular insights into CKX research in plants. © 2014 Crop Science Society of China and Institute of Crop Science, CAAS

Guan C.,Northwest Agriculture and Forestry University | Guan C.,Huazhong Agricultural University | Du X.,Yantai Academy of Agricultural science | Zhang Q.,Huazhong Agricultural University | And 3 more authors.
Frontiers in Plant Science | Year: 2017

The astringency of Chinese pollination-constant non-astringent (C-PCNA) persimmon (Diospyros kaki Thunb.) can be naturally removed on the tree. This process is controlled by a single locus and is dominant against other types of persimmons; therefore, this variant is an important candidate for commercial cultivation and the breeding of PCNA cultivars. In our previous study, six full-length coding sequences (CDS) for pyruvate kinase genes (DkPK1-6) were isolated, and DkPK1 is thought to be involved in the natural deastringency of C-PCNA persimmon fruit. Here, we characterize the eight other DkPK genes (DkPK7-14) from C-PCNA persimmon fruit based on transcriptome data. The transcript changes in DkPK7-14 genes and correlations with the proanthocyanidin (PA) content were investigated during different fruit development stages in C-PCNA, J-PCNA, and non-PCNA persimmon; DkPK7 and DkPK8 exhibited up-regulation patterns during the last developmental stage in C-PCNA persimmon that was negatively correlated with the decrease in soluble PAs. Phylogenetic analysis and subcellular localization analysis revealed that DkPK7 and DkPK8 are cytosolic proteins. Notably, DkPK7 and DkPK8 were ubiquitously expressed in various persimmon organs and abundantly up-regulated in seeds. Furthermore, transient over-expression of DkPK7 and DkPK8 in persimmon leaves led to a significant decrease in the content of soluble PAs but a significant increase in the expression levels of the pyruvate decarboxylase (DkPDC) and alcohol dehydrogenase genes (DkADH), which are closely related to acetaldehyde metabolism. The accumulated acetaldehyde that results from the up-regulation of the DkPDC and DkADH genes can combine with soluble PAs to form insoluble PAs, resulting in the removal of astringency from persimmon fruit. Thus, we suggest that both DkPK7 and DkPK8 are likely to be involved in natural deastringency via the up-regulation of DkPDC and DkADH expression during the last developmental stage in C-PCNA persimmon. © 2017 Guan, Du, Zhang, Ma, Luo and Yang.

PubMed | Yantai Academy of Agricultural science, China Institute of Technology, Chinese Academy of Agricultural Sciences, Beijing Research Center for Agricultural Standards and Testing and Northwest Agriculture and Forestry University
Type: | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2016

In the present study, the long-term and short-term cumulative risks of pyrethroids exposured for the Chinese general population and children through fruits consumption were evaluated. A total of 1450 fruit samples and seven pyrethroids were included based on the pesticide residues monitoring programme of China from 2013 to 2015. The exposure was estimated using both deterministic approach and semi-probabilistic model for comparison. The hazard index approach was used to assess cumulative risk. 26% of samples contained pyrethroid residues with concentrations ranged from 0.0050mg/kg to 1.2mg/kg, of which 30% simultaneously with 2-4 mixture residues. Results demonstrated that the cumulative health risks were extremely low for both general population and children (1-6 years old) of China in the long term. Acute risk estimations calculated by deterministic method were several or many times overestimated than the results based on semi-probabilistic method. Acute cumulative exposure of children to pyrethroid compounds in 0.76% samples were exceeded 1 in worst case scenario. More detailed assessments with adequate data in the future use probabilistic method is expected to reduce the uncertainties of cumulative dietary exposure.

PubMed | Shandong Peanut Research Institute, Yantai Academy of Agricultural science and Southwest University
Type: | Journal: Scientific reports | Year: 2016

Tillage intensities largely affect soil compaction dynamics in agro-ecosystems. However, the contribution of tillage intensities on compaction changes in underground peanut (Arachis hypogaea) fields has not been quantified. We thus aimed to better understand the role of soil tillage intensities in mitigation of compaction stress for peanuts. Using three field tillage experiments in major Chinese peanut producing areas, we quantified the effects of (1) no tillage, (2) shallow (20cm) plowing, (3) deep (30cm) plowing and (4) deep (30cm) loosening on changes in soil bulk density at 0-10cm, 10-20cm and 20-30cm depths, roots and pods growth, and nutrient accumulation. Results showed that tillage management effectively mitigated soil compaction stress for peanut growth and production. Greater beneficial improvement for the underground growth of roots and pods, and N accumulation ranked as deep plowing > shallow plowing and deep loosening. Respective increases of 7.5% and 4.6% in root biomass productions and peanut yields were obtained when soil bulk density was decreased by 0.1gcm

Wang Y.,CAS Institute of Genetics and Developmental Biology | Wang S.,Shandong Shengfeng Seeds Co. | Li H.,Shandong Shengfeng Seeds Co. | Xin Q.,Yantai Academy of Agricultural science
Plant Cell, Tissue and Organ Culture | Year: 2015

A common wheat cDNA encoding a prolyl aminopeptidase (PAP, EC was cloned by RT-PCR method and has been named as TaPAP1. The cDNA of TaPAP1 is 1,173 bp in length and encodes a predicted protein of 391 amino acids with a molecular mass and isoelectric point were 43.9 kDa and 7.94, respectively. Alignment of protein sequence indicated that TaPAP1 was evolutionarily conserved among other plants. Real-time PCR analysis revealed that TaPAP1 was predominantly expressed in seedling roots and up-regulated by various abiotic stresses, such as salt, drought, cold, copper and zinc. Compared to wild type plants, zinc-stressed TaPAP1 transgenic Arabidopsis displayed higher survival rate, the fresh weight, photosynthetic efficiency, proline levels, and PAP activity. Our results suggested that TaPAP1 controlled plant tolerance to zinc stress by means of improving the proline levels and PAP enzyme activity. Subcellular localization analysis showed that protein TaPAP1 is localized mainly in the cytoplasm. In conclusion, a novel cDNA sequence encoding wheat PAP gene was successfully obtained and the results showed that TaPAP1 is involved in the plant response to zinc stress, indicating a potential use in the transgenic breeding to improve heavy metal resistance in crop species. © 2015, Springer Science+Business Media Dordrecht.

Wang Y.-G.,CAS Institute of Genetics and Developmental Biology | Wang S.-P.,Shandong Shengfeng Seeds Co. | Li H.-J.,Shandong Shengfeng Seeds Co. | Xin Q.-G.,Yantai Academy of Agricultural science
Acta Physiologiae Plantarum | Year: 2014

Allene oxide synthase (AOS, EC is the first specific jasmonate biosynthetic pathway gene. In this study, a full-length cDNA of AOS gene was cloned from common wheat nannong 9918. The gene contains an open reading frame (1,446 bp) encoding 418 amino acids. Comparative and bioinformatic analysis revealed that the deduced protein of TaAOS was highly homologous to AOSs from other plant species. The transcript of TaAOS was found to be abundantly expressed in the flag leaves, and was up-regulated by the inoculation of B. Graminis (DC.) E.O. Speer f. sp. Tritici (Bgt) in wheat leaves. In addition, it was also induced by high concentration of NaCl and ZnCl2. When TaAOS was overexpressed in tobacco leaves via Agrobacterium tumefaciens infection, the transgenic tobacco plants displayed stronger tolerance to ZnCl2 stress compared to transgenic GFP plants. Taken together, the above facts demonstrated that TaAOS may play a role in response to diverse stresses in plants. © 2014 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.

Ge J.,Nanjing Agricultural University | Li B.,Nanjing Agricultural University | Li B.,Yantai Academy of Agricultural science | Shen D.,Nanjing Agricultural University | And 3 more authors.
BMC Plant Biology | Year: 2016

Background: Plant TRANSPARENT TESTA GLABRA (TTG) proteins regulate various developmental activities via the auxin signaling pathway. Recently, we elucidated the developmental role of tobacco (Nicotiana tabacum L.) NtTTG2 in association with 12 genes that putatively encode AUXIN RESPONSIVE FACTOR (ARF) proteins, including NtARF8, NtARF17, and NtARF19. Here we show that NtTTG2 regulates tobacco growth and development by involving the NtARF8, NtARF17, and NtARF19 genes, with the NtARF8 gene playing a predominant contribution. Results: Independent silencing of the NtARF8 gene more strongly repressed tobacco growth than silencing the NtARF17 or NtARF19 gene and more effectively eradicated the growth enhancement effect of NtTTG2 overexpression. In contrast, plant growth was not affected by silencing additional nine NtTTG2-regulated NtARF genes. In double and triple gene silencing combinations, silencing the NtARF8 gene was more effective than silencing the NtARF17 or NtARF19 gene to repress growth as well as nullify growth enhancement. Therefore, the NtARF8 predominantly cooperated with the NtARF17 and NtAFR19 of the NtTTG2 functional pathway. NtARF8 also contributed to NtTTG2-regulated seed production as concurrent NtTTG2 and NtARF8 overexpression played a synergistic role in seed production quantity, whereas concurrent silencing of both genes caused more severe seed abortion than single gene silencing. In plant cells, the NtTTG2 protein facilitated the nuclear import of NtARF8 as well as increased its function as a transcription activator. Conclusions: NtARF8 is an integral component of the NtTTG2 functional pathway, which regulates tobacco growth and development. © 2016 The Author(s).

Wang Y.,CAS Institute of Genetics and Developmental Biology | Xin Q.,Yantai Academy of Agricultural science
Plant Molecular Biology Reporter | Year: 2015

Galactinol synthase (GolS, EC, a key enzyme in the biosynthesis of raffinose family oligosaccharides (RFOs), plays roles in plant growth and developmental processes. The in vitro roles of GolS in plant responses against heavy metal stress are not well clarified. In the present study, a suppression-subtractive hybridization (SSH) cDNA library has been constructed using RNA extracted from wheat cultivar Jinan 18 treated with ZnCl2 as the tester and RNA from untreated seedlings as the driver. Sixteen expressed sequence tags (ESTs) highly homologous with known proteins associated with stress tolerance have been obtained. Among these, a 1000-bp cDNA sequence encoding GolS protein has been isolated and designated as TaGolS3. Real-time quantitative PCR (qPCR) analysis revealed that TaGolS3 was mainly expressed in young roots and upregulated by exogenous ABA treatment and several abiotic stresses, such as ZnCl2, CuCl2, low temperature, and NaCl. Subcellular localization analysis showed that TaGolS3 protein is a nuclear-localized protein. A detailed analysis of Arabidopsis and rice transgenic plants overexpressing TaGolS3 gene displayed that transgenic plants exhibited increased lateral root number, primary root length, plant survival rate, and plant height. Moreover, in comparison with the wild-type (WT) plants, the TaGolS3-overexpressing lines showed a higher expression of ROS-scavenging genes, activities of antioxidative enzymes, proline contents, and a lower level of malondialdehyde (MDA) contents and electrolyte leakage under zinc stress. These results confirmed the positive roles of TaGolS3 in improving plant tolerance to heavy metal stress, indicating a potential resource in the transgenic breeding to enhance heavy metal stress tolerance in crop plants. © 2015 Springer Science+Business Media New York

Wang L.,Ludong University | Xu H.,Ludong University | Gu L.,Yantai Academy of Agricultural science | Han T.T.,Ludong University | And 2 more authors.
Materials Technology | Year: 2016

Biosynthesis of silver nanoparticles (AgNPs) has become a promising synthetic strategy in nanotechnology in recent years. In this study, the purple sweet potato (Ipomoea batatas L.) root extract was found to have a new application for the synthesis of AgNPs. It is a natural, eco-friendly and low-cost procedure that uses purple sweet potato extract as reducing and stabilizing agent. The as-prepared AgNPs were characterized by UV–visible spectroscopy, transmission electron microscopy, atomic force microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. In addition, the biosynthesized AgNPs showed remarkable broad-spectrum antimicrobial activity against eight typical pathogens, including four clinic pathogens and four aquatic pathogens. Thus, the AgNPs synthesized with the help of purple sweet potato extract can be used as a powerful antibacterial agent and have great potential in different applications. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Zhu Q.,Nanjing Agricultural University | Li B.,Nanjing Agricultural University | Li B.,Yantai Academy of Agricultural science | Mu S.,Nanjing Agricultural University | And 5 more authors.
BMC Genomics | Year: 2013

Background: The phytohormone auxin mediates a stunning array of plant development through the functions of AUXIN RESPONSE FACTORs (ARFs), which belong to transcription factors and are present as a protein family comprising 10-43 members so far identified in different plant species. Plant development is also subject to regulation by TRANSPARENT TESTA GLABRA (TTG) proteins, such as NtTTG2 that we recently characterized in tobacco Nicotiana tabacum. To find the functional linkage between TTG and auxin in the regulation of plant development, we performed de novo assembly of the tobacco transcriptome to identify candidates of NtTTG2-regulated ARF genes.Results: The role of NtTTG2 in tobacco growth and development was studied by analyzing the biological effects of gene silencing and overexpression. The NtTTG2 gene silencing causes repressive effects on vegetative growth, floral anthocyanin synthesis, flower colorization, and seed production. By contrast, the plant growth and development processes are promoted by NtTTG2 overexpression. The growth/developmental function of NtTTG2 associates with differential expression of putative ARF genes identified by de novo assembly of the tobacco transcriptome. The transcriptome contains a total of 54,906 unigenes, including 30,124 unigenes (54.86%) with annotated functions and at least 8,024 unigenes (14.61%) assigned to plant growth and development. The transcriptome also contains 455 unigenes (0.83%) related to auxin responses, including 40 putative ARF genes. Based on quantitative analyses, the expression of the putative genes is either promoted or inhibited by NtTTG2.Conclusions: The biological effects of the NtTTG2 gene silencing and overexpression suggest that NtTTG2 is an essential regulator of growth and development in tobacco. The effects of the altered NtTTG2 expression on expression levels of putative ARF genes identified in the transcriptome suggest that NtTTG2 functions in relation to ARF transcription factors. © 2013 Zhu et al.; licensee BioMed Central Ltd.

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