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Yangzhou, China

Yangzhou University is a university in Jiangsu Province, China. It grew out of a merger in 1992 of six existing colleges. The school has around 2,000 faculty and 31,000 undergraduates. It offers 92 undergraduate programs covering 11 disciplines.The programs and research include humanity, science, business, engineering, architecture, computer, medicine, art, music, agriculture, animal husbandry, etc.Around 2,000 faculty members work on campus, including around 1000 professors and associate professors. 97 faculty members are entitled to State Fund for Specialists, 29 are entitled as Outstanding Contribution-making Young Experts by the government. At undergraduate level, the university has over 16,000 adult students in its continued education programs. Undergraduate programs are also available for students from Hong Kong, Macao and Taiwan. Students from foreign countries can be seen in this program as well.At the graduate level, around 3800 graduate students are studying in 21 doctoral and 122 master's programs, in which around 748 faculties are involved as doctoral and master's program supervisors.YZU has one national key laboratory, 14 ministerial and provincial key laboratories, and 37 teaching laboratory centers.YZU has seven libraries with 3.25 million volumes of books and journals. The libraries have a collection of rare Chinese classics. The university has its own Yangzhou University Journal , Jiangsu Agricultural Research, Jiangsu Clinical Medicine Journal and Yangzhou University Cuisine Journal.YZU closely combines industry, teaching and research. There are over 900 research projects underway at the university, including 65 national projects listed in 863 Hi-tech Projects, 973 High-tech Projects and National Priority Projects for the Ninth Five-Year Plan, and 144 provincial- or ministerial-level research projects. More than 260 research accomplishments have received awards at provincial or national level. The annual research fund amounts to over 120 Million RMB, ranking far above other universities of the same level.YZU has established ties with institutions of higher education in 10 countries. More than 100 foreign language teachers and experts and over 2500 foreign scholars have taught, delivered lectures or participated in academic exchange programs over the past few years.Over 700 faculty members have been sent abroad for academic visits, advanced studies, research cooperation or attendance at international academic conferences. Besides the exchanges at the faculty and researchers level, exchanges at the student level are developing. The number of international students in Yangzhou University is on the rise as is the number of Chinese students learning English. Wikipedia.


Shen J.J.,Yangzhou University
Advanced Materials Research | Year: 2014

To utilize time-space resource of intersections efficiently, signal timing based on efficiency optimization was studied. The number of vehicles and speed were selected as two parameters constructing efficiency model for four-phase intersections, the relation model between these two parameters was developed basing on field data. The volume corresponding to optimal efficiency through derivation and signal timing on the basis of this volume were obtained by applying Synchro software. Conclusion through an actual case indicates that this method can achieve minimum delay on the premise of efficiency optimization and make the performance of signalized intersection better. © (2014) Trans Tech Publications, Switzerland. Source


Liu C.,Yangzhou University | Bhole S.D.,Ryerson University
Science and Technology of Welding and Joining | Year: 2013

Recent economic and political events have further highlighted the need for new and strategically accessible sources of oil and gas. With the continually increasing demand for oil and gas, the requirement for pipeline steels with higher strength, toughness and weldability has been one of the most important factors driving the development of high strength pipeline steels, particularly with the oil exploration proceeding into arctic and deep sea regions, enhancing the weldability and mechanical properties of the new pipeline steels and weld consumables. Developments in the welding processes for manufacture and field welding are described in terms of process principles, equipment, consumables, weld quality, process economics and further developments. The increasing and changing requirement for weldability and mechanical properties in the heat affected zone and weld metal of pipeline welds are presented along with the reported solutions to the problems. © 2013 Institute of Materials, Minerals and Mining. Source


Wu X.,Shanghai Institute of Technology | He J.,Yangzhou University | Chen J.,Shanghai Institute of Technology | Yang S.,Shanghai Institute of Technology | Zha D.,Shanghai Institute of Technology
Protoplasma | Year: 2014

Cytokinins were recently shown to control plant adaptation to environmental stresses. To characterize the roles of cytokinins in the tolerance of eggplant (Solanum melongena Mill.) to salt stress, the protective effects of 6-benzyladenine (6-BA) on the growth, photosynthesis, and antioxidant capacity in the leaves of two eggplant cultivars Huqie12 (salt-sensitive) and Huqie4 (salt-tolerant) were investigated. Under 90 mM NaCl stress, Huqie4 showed higher biomass accumulation and less oxidative damage compared to the Huqie12. Application of exogenous 10 μM 6-BA significantly alleviated the growth suppression caused by salt stress in two eggplant genotypes. In parallel with the growth, 6-BA application in salt-stressed plants resulted in enhanced chlorophyll contents, as well as photosynthetic parameters such as net CO2 assimilation rate (P n), stomatal conductance (g s), transpiration rate (E), and intercellular CO2 concentration (C i). Furthermore, exogenous 6-BA also significantly reduced the O2 - production rate and malondialdehyde content and markedly increased the antioxidant enzymes superoxide dismutase and peroxidase, the antioxidant metabolites ascorbate and reduced glutathione (GSH), and proline in both genotypes under salt stress. The results indicate that exogenous 6-BA is useful to improve the salt resistance of eggplant, which is most likely related to the increase in photosynthesis and antioxidant capacity. © 2013 Springer-Verlag Wien. Source


Liu Y.,Jiangnan University | Sun J.,Jiangsu University | Rao S.,Yangzhou University | Su Y.,Jiangnan University | Yang Y.,Jiangnan University
Food and Chemical Toxicology | Year: 2013

It is the first time to extract polysaccharides (CVPs) from Catathelasma ventricosum. The antihyperglycemic and antioxidant activity of CVPs in streptozotocin-induced diabetic mice were examined. Compared with untreated diabetic mice, the administration of CVPs for 30days caused a significant decrease in the concentrations of blood glucose, total cholesterol (TC), triglycerides (TGs), low-density lipoprotein-cholesterol (LDL-C) and maleic dialdehyde (MDA), and a significant increase in the concentrations of high density lipoprotein-cholesterol (HDL-C) and the activities of antioxidant enzymes. Specially, when normal mice were treated with CVPs, all detection indexes and pathologic morphologies of liver, kidney and pancreas are similar to untreated normal mice, which indicated CVPs are safe for normal mice. In addition, the average molecular weight of CVPs was estimated to be from 3.7×103 to 1.7×107Da and they were mainly composed of glucose (93.5%) with the conformation of α-d-Glucopyranose. © 2013 Elsevier Ltd. Source


Yang J.,CAS Shanghai Institute of Applied Physics | Shi G.,CAS Shanghai Institute of Applied Physics | Tu Y.,Yangzhou University | Fang H.,CAS Shanghai Institute of Applied Physics
Angewandte Chemie - International Edition | Year: 2014

Recent experiments have shown the coexistence of both large unoxidized and oxidized regions on graphene oxide (GO), but the underlying mechanism for the formation of the GO atomic structure remains unknown. Now, using density functional calculations, 52 oxidation pathways for local pyrene structures on GO were identified, and a kinetic profile for graphene oxidation with a high correlation between oxidation loci was proposed, which is different from the conventional view, which entails a random distribution of oxidation loci. The high correlation is an essential nature of graphene oxidation processes and can be attributed to three crucial effects: 1) breaking of delocalized π bonds, 2) steric hindrance, and 3) hydrogen-bond formation. This high correlation leads to the coexistence of both large unoxidized and oxidized regions on GO. Interestingly, even in oxidized regions on GO, some small areas of sp2-hybridized domains, similar to "islands", can persist because of steric effects. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Lei Z.,Nanjing Southeast University | Jiang Y.,Yangzhou University | Li T.,Nanjing Southeast University | Zhu J.,Nanjing Southeast University | Zeng S.,Nanjing Southeast University
Journal of Neuropathology and Experimental Neurology | Year: 2011

Glial cell line-derived neurotrophic factor (GDNF) and its receptor GFRα1 have been implicated in the survival of ventral midbrain dopaminergic (DA) neurons, but the molecular mechanisms bywhich GDNF generates DA neurons in grafted midbrain-derived neural stem cells (mNSCs) are not understood. Midbrain-derived neural stem cells isolated from rat embryonic mesencephalon (embryonic day 12) were treated with GDNF or in combination with GFRα1 small interfering RNA. Reverse transcription-polymerase chain reaction, Western blot, and immunocytochemistry were used totest the expression of the orphan nuclear receptor Nurr1 and thetranscription factor Pitx3 and newborn tyrosine hydroxylase (TH)-positive cells. Treatment of mNSCs with GDNF increased mNSCs' sphere diameter, reduced expression of caspase 3, and increased expression of Bcl-2. Glial cell line-derived neurotrophic factor-treated mNSCs enhanced Nurr1 and Pitx3 expression and the fraction of TH-, TH/Pitx3-, and TH/Nurr1-positive cells in culture. Grafted GDNF-treated mNSCs significantly decreased apomorphine-induced rotation behavior in 6-hydroxydopamine-lesioned rats. Glialcell line-derived neurotrophic factor-treated mNSCs showed increased numbers of TH/Pitx3-and TH/Nurr1-postivie cells. The effect elicited by GDNF was inhibited by small interfering RNA-mediated knockdown of GFRα1. Our data demonstrate the contribution of GDNF to DA neuron development and may also elucidate pathogenetic mechanisms in Parkinson disease and contribute to the development of novel therapies for the disorder. © 2011 by the American Association of Neuropathologists, Inc. Source


Huang T.,Xian University of Architecture and Technology | Ma Y.,Xian University of Architecture and Technology | Cong H.,Yangzhou University | Tan P.,Xian University of Architecture and Technology
Desalination and Water Treatment | Year: 2014

The technology of water-lifting and aeration can be widely applied in water aeration, algal inhibition, and endogenous pollution control. The Jin-Pen reservoir has been undergoing increasing water pollution in the past few years. To solve this problem, the water-lifting aerator system was installed here in the summer of 2010, and its improving effect on water quality was investigated. The results showed that under the mixing and aeration conditions, the thermal stratification structure was disturbed but not destroyed. The vertical water temperature difference was reduced by 3-5°C, and the surface temperature fell at 2.1-3.6°C. The seasonal aerobic state was withheld at the bottom of the reservoir, and the concentration of DO was maintained above 2 mg/L at the sediment surface. The decay rate of DO in the hypolimnion decreased by 51.2 and 49.6%, respectively, compared to the same period in 2008 and 2009. The concentrations of TP and NH3-N in the overlying water on the sediment surface decreased to 0.12 and 0.25 mg/L, respectively. The algal abundance in the surface water was reduced by more than 75-80%. However, the present cost of water improvement considering the current water supply capacity (80 × 104 m3) was only 0.004 Yuan RMB/m3. © 2013 © 2013 Balaban Desalination Publications. All rights reserved. Source


Rao S.,Yangzhou University | Sun J.,Jiangnan University | Liu Y.,Jiangnan University | Zeng H.,Angel Yeast Co. | And 2 more authors.
Food Chemistry | Year: 2012

Lysozyme from hen egg white is a well-known antimicrobial protein with high ratio of hydrophobic and positively charged amino acid residues. In order to explore functional bioactivities of enzymatic hydrolysates of lysozyme, the protein was subjected to a simulated gastrointestinal digestion and the resulting hydrolysate (LPH2) showed a strong competitive angiotensin I-converting enzyme (ACE) inhibitory activity (IC50 = 12.6 μg/ml) and a remarkable antioxidant activity. The LPH2 was fractionated using a 3 kDa cut-off membrane and the obtained permeate LPH2-3 kDa was analysed by MALDI-TOF-TOF MS. Using this technology, 38 different peptides were identified and some of these peptides were well fit with structure requirements of ACE inhibitory peptides and/or antioxidant peptides. The findings from this study suggest that the protein containing high proportion of hydrophobic and positively charged residues have the potential to generate multifunctional peptides, and these peptides would be beneficial ingredient to be used in functional foods. © 2012 Elsevier Ltd. All rights reserved. Source


Zhu Y.M.,Yangzhou University
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] | Year: 2012

Based on the complete genome sequence of pigeon-origin Newcastle disease virus strain JS/07/04/ Pi(genotype VIb), nine overlapped fragments covering its full-length genome were amplified by RT-PCR. The fragments were connected sequentially and then inserted into the transcription vector TVT7/R resulting in the TVT/071204 which contained the full genome of strain JS/07/04/Pi. The TVT/071204 was co-transfected with three helper plasmids pCI-NP, pCI-P and pCI-L into the BSR cells, and the transfected cells and culture supernatant were inoculated into 9-day-old SPF embryonated eggs 60 h post-transfection. The HA and HI tests were conducted following the death of embryonated eggs. The results showed that the allantoic fluids obtained were HA positive and the HA could be inhibited by anti-NDV serum which indicated that the strain JS/07/04/Pi was rescued successfully. The rescued virus rNDV/071204 showed similar growth kinetics to its parental virus in CEF. The successful recovery of this strain would contribute to the understanding of the host-specificity of pigeon-origin NDV and to the development of the novel vaccines against the NDV infection in pigeons. Source


Chu H.J.,Yangzhou University | Chu H.J.,University of Akron | Chu H.J.,Los Alamos National Laboratory | Pan E.,University of Akron | And 3 more authors.
Journal of the Mechanics and Physics of Solids | Year: 2012

By applying semi-analytical point-force Greens functions obtained via the Stroh formulism, we derive simple line integrals to calculate the elastic displacement and stress fields for a three-dimensional dislocation loop in an anisotropic bimaterial system. The solutions for the case of anisotropy are more convenient for treating an arbitrary dislocation loop compared with traditional area integration. With this new formulation, we numerically examine the displacement, stress, and energy due to the interaction between a dislocation loop and the bimaterial interface in an AlCu system. The interactive image energy due to the elastic moduli mismatch across the interface is then numerically evaluated. The result shows that a dislocation loop is subjected to an attractive force by the interface when it lies in the stiff material, and a repulsive force when it lies in the soft material. Moreover, the dependence of the interactive image energy of a dislocation loop on the position and size of the dislocation loop are also demonstrated and discussed. Significantly, it is found that the interactive image energy for a dislocation loop depends only on the ratio d/a, where a is the loop diameter and d is its distance to the interface. The examples studied provide benchmark solutions for anisotropic bimaterial dislocation problems. Source


Shen Q.,Nanjing University of Aeronautics and Astronautics | Shen Q.,Yangzhou University | Jiang B.,Nanjing University of Aeronautics and Astronautics | Cocquempot V.,Lille University of Science and Technology
Proceedings of the Institution of Mechanical Engineers. Part I: Journal of Systems and Control Engineering | Year: 2012

This paper addresses a practical issue in fault diagnosis and estimation for a near-space hypersonic vehicle (NSHV) with sensor faults. First, the Takagi-Sugeno (T-S) fuzzy model is established to represent the NSHV non-linear dynamics, and sliding mode observers (SMO) are designed to generate a bank of residuals. Then, a novel sensor fault model is proposed, which contains time-varying bias faults and time-varying gain faults. Further, based on the Lyapunov stability theory, novel fault diagnostic algorithms are given to estimate the sensor fault, which removes the two classical assumptions in existing works that the time derivative of the output errors should be known and that the system states should be bounded. In addition, a sufficient condition for the existence of SMO is derived in the sense of the Lyapunov stability theory, which can be easily solved by Matlab« LMI toolbox. Finally, simulation results are presented to demonstrate the efficiency of the proposed approach. © IMechE 2012. Source


Chen M.,Key Laboratory of Environmental Materials and Environmental Engineering of Jiangsu Province | Wang J.,Key Laboratory of Environmental Materials and Environmental Engineering of Jiangsu Province | Zhang W.,Key Laboratory of Environmental Materials and Environmental Engineering of Jiangsu Province | Diao G.,Yangzhou University
Journal of Electroanalytical Chemistry | Year: 2013

A new water-solution inclusion complex of imidacloprid (IDP) with β-cyclodextrin polymer (β-CDP) was prepared by a facile strategy and characterized by FTIR, powder X-ray diffractometry, thermogravimetry, 1H NMR spectroscopy and UV-vis spectroscopy. The ratio of β-cyclodextrin (β-CD) unit in β-CDP to IDP is determined as 2:1. At 25 °C, the dissociated constant of IDP-β-CDP is measured as 1.79 ± 0.17 × 10-5 M2 by UV-vis spectroscopy. The well behaved electrochemical properties of IDP-β-CDP in water are observed. The diffusion coefficient of reduced state and the diffusion activation energy are calculated as 4.0 ± 0.5 × 10-7 cm2 s -1 and 9.8 ± 0.3 kJ mol-1, respectively. Compared with IDP, the solubility of IDP-β-cyclodextrin polymer (IDP-β-CDP) is greatly enhanced due to the water-soluble β-CDP host. © 2013 Elsevier Ltd. All rights reserved. Source


Yu G.,Yangzhou University | Zheng Q.-S.,Shanghai University | Li G.-F.,Shanghai University
AAPS Journal | Year: 2014

Physiologically based pharmacokinetic (PBPK) modeling holds great promise for anticipating the quantitative changes of pharmacokinetics in pediatric populations relative to adults, which has served as a useful tool in regulatory reviews. Although the availability of specialized software for PBPK modeling has facilitated the widespread applications of this approach in regulatory submissions, challenges in the implementation and interpretation of pediatric PBPK models remain great, for which controversies and knowledge gaps remain regarding neonatal development of the gastrointestinal tract. The commentary highlights the similarities and differences in the gastrointestinal pH and transit time between neonates and adults from a PBPK modeling prospective. Understanding the similarities and differences in these physiological parameters governing oral absorption would promote good practice in the use of pediatric PBPK modeling to assess oral exposure and pharmacokinetics in neonates. © 2014 American Association of Pharmaceutical Scientists Source


Jin W.-L.,Yangzhou University
Jisuanji Xuebao/Chinese Journal of Computers | Year: 2012

Suppose we are given an unsorted database of size N. Whereas the extremely complicated calculations make it almost impossible to establish a precise mathematical formulation to describe the conclusion that for a uniform initial amplitude distribution, there exist two sets of the phase rotation angles defined in the real domain such that a unique desired state can be found with certainty in a two-dimensional complex subspace, we resorted to an approximate computational method for simplifying the calculations and thus derived the multiphase matching equation by induction. This equation guarantees that a unique desired state can be found with high success probability provided the number j of elements in one of the sets of the phase rotation angles is relatively small compared to N (N is sufficiently large). In this case, for any given j>2, we analyze the computational complexity of Long algorithm by exploiting a recurrence relation derived in this paper. Finally, we further verify the validity of the multiphase matching equation by some examples of numerical simulation. Source


Yan D.,Yangzhou University
Paiguan Jixie Gongcheng Xuebao/Journal of Drainage and Irrigation Machinery Engineering | Year: 2012

Based on the existing formulas and those proposed by the author for pump and pumping system performance (efficiency and NPSHc) conversion, a further mathematical derivation was introduced and a group of universal conversion formula was developed in the paper. The universal formulas were applicable for head, flow rate, efficiency and NPSHc. The head curves of both pump and pumping system were presented in a quadratic polynomial, and the head coefficients were calculated by using the least square method. The overall efficiency formula was detailed and the method for determining the constant involved the formula was introduced. Also, a universal NPSHc formula, which is applicable to any operating points, and where the shock loss effect is include, was provided in this paper. The approach for determining the coefficient in that formula was proposed as well. The universal formulas for head, flow rate, efficiency and NPSHc were derived according to flow similarity in pumps and by means of the affinity laws of pump, and presented with head coefficient, efficiency constant and NPSHc coefficient. All the formulas, their coefficients and constants were integrated into one formula, respectively; thus they were considered as universal ones, which are applicable to both model and prototype pumps. The universal formulas can be used to estimate pump and pumping system performance and carry out performance conversion from model to prototype. Source


Shen Q.,Nanjing University of Aeronautics and Astronautics | Shen Q.,Yangzhou University | Jiang B.,Nanjing University of Aeronautics and Astronautics | Cocquempot V.,Lille University of Science and Technology
International Journal of Robust and Nonlinear Control | Year: 2013

The problem of fault-tolerant control (FTC) for a class of uncertain nonlinear systems with actuator faults is discussed, and an observer-based FTC scheme is proposed. Adaptive fuzzy observers are designed to provide a bank of residuals for fault detection and isolation. Using a backstepping approach, we proposed a novel fault diagnosis algorithm, which removes the classical assumption that the time derivative of the output error should be known. Further, an accommodation scheme is proposed to compensate for the effect of the fault, where it is not needed to know the bounds of the time derivative of the fault. The proposed controller guarantees that all signals of the closed-loop system are semi-globally uniformly ultimately bounded and converge to a small neighborhood of the origin by appropriately choosing designed parameters. In addition, a sufficient condition for the existence of an fault detection and isolation observer is derived using Lyapunov stability theory. Finally, a numerical example and a practical aircraft longitudinal motion dynamics are used to demonstrate the effectiveness of the proposed FTC approach. Copyright © 2013 John Wiley & Sons, Ltd. Source


Wang F.-B.,Nanjing University | Wang F.-B.,Yangzhou University | Wang J.,Nanjing University | Shao L.,Nanjing University | And 2 more authors.
Electrochemistry Communications | Year: 2014

An approach to the synthesis of gold nanoparticles-graphene (AuNPs-GOSH) hybrids via the AuS bond between the sulfhydryl-functionalized graphene oxide (GOSH) and gold nanoparticles (AuNPs) has been proposed. In this approach, the sulfhydryl groups highly dispersed on the GOSH nanosheets are used to assemble gold nanoparticles, forming the AuNPs-GOSH hybrids. It is found that the AuNPs-rGO hybrids show high electrochemical activity toward the oxygen reduction reaction (ORR) and high stability in alkaline media. © 2013 Elsevier Ltd. All rights reserved. Source


Jin H.,Nanjing Southeast University | Jiang Y.,Yangzhou University | Wei Q.,Nanjing Southeast University | Chen L.,Nanjing Southeast University | Ma G.,Nanjing Southeast University
NeuroRehabilitation | Year: 2013

Objective: Using heart rate recovery (HRR) after exercise as an index of autonomic function, we evaluate the effects of aerobic cycling training on HRR and cardiovascular fitness (peak VO2) in chronic stroke patients and investigate the relationship between changes in HRR and those in peak VO 2. Methods: 128 participants with chronic stroke were randomized to a 12-week (5×/week) progressive aerobic cycling training group (n = 65) or a control group (n = 63). Peak VO2, muscle strength, 6-minute walking distance (6MWD) and HRR were measured before and after the intervention. Results: Cycling training leads to significant increase in peak VO2, HRR, muscle strength and 6MWD. In the cycling group, percent changes in peak VO2 were positively associated with those in paretic and nonparetic muscle strength and HRR. Linear regression revealed that percent increases in peak VO2 were significantly correlated with percent changes in HRR when controlling for pre-peak VO2, age, gender, duration since stroke and improved muscle strength. Conclusion: Aerobic cycling training can favorably modify HRR in stroke survivors. Rapid HRR, as an indicator of enhanced autonomic function, is useful for predicting gains in cardiovascular fitness. These findings indicate the underlying importance of autonomic modulation on cardiovascular adaptations to stroke exercise rehabilitation. © 2013 - IOS Press and the authors. All rights reserved. Source


Huo Z.,China Agricultural University | Feng S.,China Agricultural University | Feng S.,Yangzhou University | Kang S.,China Agricultural University | Dai X.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research
Journal of Arid Environments | Year: 2012

We trained and tested artificial neural network (ANN) models for reference evapotranspiration (ET 0) using 50 years' meteorological data from three stations in northwest China. Multiple linear regressions (MLRs), the Penman equation, and two empirical equations were used to compare the performance of the ANNs. A connection weight method was used to quantify the importance of climate factors in performance. In addition, the error changes of the ANNs with seasons were evaluated according to absolute error, variance, and coefficient of variance. Results showed that in arid and semi-arid areas, the ANNs in which the climate data were used successfully estimated ET 0, and the ANNs with five inputs were more accurate than those with four or three. Relative to the MLRs, the Penman equation, and empirical equations, the ANNs exhibited high precision. Maximum air temperature, minimum air temperature, and relative humidity were the most crucial input of ANN-based ET 0 estimation for arid and semi-arid areas. In the study area, the importance of these three climate factors accounted respectively for 39.82-46.64%, 28.48-33.46%, and 10.73-26.17% to estimation of ET 0. Generally, ANNs underestimated ET 0 from January to July and overestimated it from August to December. © 2012. Source


Wei C.,Yangzhou University | Wei C.,Qufu Normal University | Zhang Y.,Qufu Normal University
Mathematical Problems in Engineering | Year: 2015

Entropy measure is an important topic in the fuzzy set theory and has been investigated by many researchers from different points of view. In this paper, two new entropy measures based on the cosine function are proposed for intuitionistic fuzzy sets and interval-valued intuitionistic fuzzy sets. According to the features of the cosine function, the general forms of these two kinds of entropy measures are presented. Compared with the existing ones, the proposed entropy measures can overcome some shortcomings and be used to measure both fuzziness and intuitionism of these two fuzzy sets; as a result, the uncertain information of which can be described more sufficiently. These entropy measures have been applied to assess the experts' weights and to solve multicriteria fuzzy group decision-making problems. © 2015 Cuiping Wei and Yuzhong Zhang. Source


Gu X.,The University of Oklahoma Health Sciences Center | Gu X.,Yangzhou University | Strom K.,The University of Oklahoma Health Sciences Center | Spaliviero M.,The University of Oklahoma Health Sciences Center | Wong C.,The University of Oklahoma Health Sciences Center
Journal of Endourology | Year: 2011

Purpose: GreenLight HPS™ laser photoselective vaporization prostatectomy (PVP) is a treatment option for lower urinary tract symptoms secondary to benign prostatic hyperplasia. We review our experience using the 120W GreenLight HPS laser system. Materials and Methods: We prospectively evaluated our experience with GreenLight HPS laser PVP. All patients who failed medical therapy underwent GreenLight HPS laser PVP (C.W.). All had American Urological Association Symptom Score, Quality of Life score, Sexual Health Inventory for Men, American Society of Anesthesiologists risk score, serum prostate-specific antigen, maximum flow rate, and postvoid residual determinations and volumetric measurements with transrectal ultrasonography. Transurethral PVP was performed using a GreenLight HPS side-firing laser system. Results: 170 consecutive patients were identified, having a mean age of 67.4±9.5 years. The mean prostate volume was 65.3±36.7 mL and mean American Society of Anesthesiologists score was 2.3±0.7. Mean laser time, operating time, and energy usage were 14.1±10.5 minutes, 33.5±24.7 minutes, and 95.0±72.3 kJ, respectively. All were outpatient procedures with 100 (58.8%) patients catheter-free at discharge. 35 (20.5%) and 26 (15.3%) patients had follow-up of 24 and 36 months, respectively. Eight (4.7%) patients developed a urinary tract infection. No bladder neck contracture or urethral stricture were reported. American Urological Association Symptom Score, Quality of Life, maximum flow rate, and postvoid residual showed immediate and stable improvement from baseline (p<0.05) that has been durable to 36 months. The Sexual Health Inventory for Men score did not change postoperatively. Conclusions: Our intermediate results suggest that GreenLight HPS laser PVP is safe, effective, and durable for the treatment of lower urinary tract symptoms secondary to benign prostatic hyperplasia. © Copyright 2011, Mary Ann Liebert, Inc. Source


Although hyperbaric oxygen (HBO) therapy has been reported to help heal chronic foot ulcers in patients with diabetes mellitus (DM), production of HBO-related oxidative stress is a concern. To assess the therapeutic effect and oxidative stress of HBO, a 2-week, prospective, randomized, controlled clinical study was conducted from January 1, 2010 to January1, 2012 among 36 consecutively admitted patients with diabetic foot ulcers (DFU). Average patient age was 60.08 ± 5.97 years and average DM duration was 16.4 ± 11.3 years; 86.1% had type 2 DM, and 47.2% had Wagner grade-III foot ulcers. Patients randomized to the control group (n = 18) received standard care including offloading, wound debridement, and glucose control. HBO treatment group patients (n = 18) received standard care and twice-daily HBO sessions for 90 minutes at 2.5 atmospheres absolute (ATA) 5 days a week for 2 weeks. Transcutaneous oxygen pressure (TcPo2) at the edge of the ulcer and wound size were measured at baseline and after 7 and 14 days of treatment. Ulcer tissues were harvested on days 7 and 14 to determine oxidative stress by measuring malondialdehyde (MDA) and antioxidant enzyme (superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx]) levels. Compared to baseline, TcPo2 in the HBO group increased on day 7 (477.8 ± 118.2 mm Hg versus 37.06 ± 5.23 mm Hg, P <0.01) and day 14 (501.1 ± 137.7 mm Hg versus 35.61 ± 4.85 mm Hg, P <0.01). Ulcer size reduction in the HBO group was greater than that of the control group (42.4% ± 20.0% versus 18.1% ± 6.5%, P <0.05). MDA levels, SOD, and CAT were all significantly higher in the HBO than in the control group on day 14 (P<0.05). The results of this study suggest HBO treatment for 2 weeks initiates a healing response in chronic DFUs, but the observed oxidative stress in local ulcer tissue may offset this effect long-term. Until needed additional research has been conducted, prolonged and/or inappropriate HBO treatment should be avoided. Source


Tang B.,Yangzhou University | Lu X.,Tsinghua University | Ye L.,Tsinghua University | Shi W.,Tsinghua University
Earthquake Engineering and Engineering Vibration | Year: 2011

According to the Code for Seismic Design of Buildings (GB50011-2001), ten typical reinforced concrete (RC) frame structures, used as school classroom buildings, are designed with different seismic fortification intensities (SFIs) (SFI=6 to 8. 5) and different seismic design categories (SDCs) (SDC=B and C). The collapse resistance of the frames with SDC=B and C in terms of collapse fragility curves are quantitatively evaluated and compared via incremental dynamic analysis (IDA). The results show that the collapse resistance of structures should be evaluated based on both the absolute seismic resistance and the corresponding design seismic intensity. For the frames with SFI from 6 to 7. 5, because they have relatively low absolute seismic resistance, their collapse resistance is insufficient even when their corresponding SDCs are upgraded from B to C. Thus, further measures are needed to enhance these structures, and some suggestions are proposed. © 2011 Institute of Engineering Mechanics, China Earthquake Administration and Springer-Verlag Berlin Heidelberg. Source


Chen Y.,Jiangsu University | Chen Y.,Yangzhou University | Quan L.,Jiangsu University | Zhu X.,Jiangsu University
IEEE Transactions on Magnetics | Year: 2012

In this paper, a new double-rotor stator permanent-magnet (DR-SPM) motor is proposed for hybrid electric vehicles, which consists of both armature windings and PMs in the stator and inner rotor while no PM in the middle rotor. This motor not only inherits the merits of simple rotor structure, high reliability and high power density, but also can avoid the middle rotor's cooling problem effectively. The motor topology and operation principle are presented and by using a finite element method (FEM), the electromagnetic performance of the DR-SPM motor is investigated. To verify the FEM results, a 2 kW prototype motor is newly designed and manufactured. Finally, some experiments are conducted to evaluate performance of the motor. © 2012 IEEE. Source


Fei M.,Soochow University of China | Hang Q.,Nantong University | Hang Q.,Tohoku Pharmaceutical University | Hou S.,Yangzhou University | Ruan C.,Soochow University of China
International Journal of Hematology | Year: 2013

Spy1 is a member of the Speedy/Ringo family, which regulates cell proliferation and survival. Spy1 has been demonstrated to promote the cell-cycle progress through p27Kip1 degradation. Previous investigations have suggested cell adhesion-mediated drug resistance (CAM-DR) in multiple myeloma (MM) is a primary factor for minimal residual disease (MRD) leading to relapse after chemotherapy. However, the precise mechanism remains elusive. In this study, we used MM cell lines to determine whether Spy1 plays a role in CAM-DR. We demonstrated that adhesion of MM cells to fibronectin (FN) decreased Spy1 expression. Overexpression of Spy1 did not affect MM cells adhesion to FN, but did reverse the doxorubicin- or mitoxantrone-induced CAM-DR phenotype. Spy1 protein level was also correlated with reciprocal up-regulation of p27 Kip1 when RPMI 8226 cells bound FN. Spy1 overexpression promoted p27Kip1 phosphorylation at T187, then induced the p27Kip1 degradation in the adhesion model. In addition, increasing p27Kip1 level or disturbing p27Kip1 phosphorylation at T187 abolished the CAM-DR reversion when Spy1 was overexpressed. Collectively, our data suggest that Spy1 plays an important role in CAM-DR, which depends on the function of p27Kip1. Our findings provide a rational framework for further development of Spy1 as a novel target for MM therapy. © 2013 The Japanese Society of Hematology. Source


Li S.-R.,Yangzhou University | Fu X.-H.,Lanzhou University of Technology | Batra R.C.,Virginia Polytechnic Institute and State University
Mechanics Research Communications | Year: 2010

We study free vibrations of a simply supported three-layer circular cylindrical shell with the inner and the outer layers made of the same homogeneous material and the middle layer composed of a functionally graded material. We use Flügge's shell theory to derive governing equations, express mid-plane displacements in terms of trigonometric functions that identically satisfy the boundary conditions, and compute natural frequencies in terms of the geometrical and the material parameters. Computed results show that the fundamental natural frequency decreases with an increase in the radius-to-thickness ratio, and increases with an increase in the ratio of Young's modulus at the mid-surface to that of the outer (or the inner) layer. © 2010 Elsevier Ltd. All rights reserved. Source


Huang D.,Zhejiang University | Xu F.,Zhejiang University | Chen T.,Yangzhou University | Wang Y.,Zhejiang University | Lin X.,Zhejiang University
RSC Advances | Year: 2013

Chiral SPINOL-phosphoric acids exhibited high activities and excellent diastereo- and enantioselectivities in the catalysis of the three-component Povarov reaction of benzyl N-vinylcarbamate, anilines and aldehydes with a catalyst loading of 5 mol%. The synthetic utility of this protocol was demonstrated by the asymmetric synthesis of enantioenriched benzo[e]indolizidine with excellent yield and excellent level of sterecontrol. © 2013 The Royal Society of Chemistry. Source


Poly(aniline-co-diphenylamine and 5-aminosalicylic acid) with p-type and n-type doping segments is first synthesized via electrochemical copolymerization of aniline, diphenylamine (DPA) and 5-aminosalicylic acid (5-ASA) in a solution of N, N-dimethylformamide (DMF) and sulfuric acid; a potential separation between two doping states is ≥1 V, depending slightly on pH in a pH range of 4.0 to 13.3. For simplicity, the product of the electrochemical copolymerization, i.e., poly(aniline-co-diphenylamine and 5-aminosalicylic acid), is called copolymer. Evidence for both doping types comes from results of cyclic voltammetry of the copolymer in the wide pH range and wide sweeping potential region. The p-type doping state of the copolymer is not only related to aniline units in the copolymer chain, but also related to the potential region of the cathodic scan. The copolymer is a mixture of oligomers that are proven by mass spectral analysis. The measurements of electron spin resonance (ESR) demonstrate that free radicals are present in the copolymer. In particular, the copolymer shows evident photoelectric response under visible light illumination in aqueous media. © 2014 Elsevier Ltd. All rights reserved. Source


Wang J.,Tangshan College | Hua I.,Yangzhou University
Neural Processing Letters | Year: 2011

In the past few decades, many face recognition methods have been developed. Among thesemethods, subspace analysis is an effective approach for face recognition. Unsuperviseddiscriminant projection (UDP) finds an embedding subspace that preserves local structure information, and uncovers and separates embedding corresponding to different manifolds. Though UDP has been applied in many fields, it has limits to solve the classification tasks, such as the ignorance of the class information. Thus, a novel subspace method, called supervised discriminant projection (SDP), is proposed for face recognition in this paper.In our method, the class information was utilized in the procedure of feature extraction.In SDP, the local structure of the original data is constructed according to a certain kind of similarity between data points, which takes special consideration of both the localinformation and class information. We test the performance of the proposed method SDP on three popular face image databases (i.e. AR database, Yale database, and a subset of FERET database). Experimental results show that the proposed method is effective. © Springer Science+Business Media, LLC. 2011. Source


Yang L.,Jiangsu University | Xu L.,Yangzhou University
Information Sciences | Year: 2013

Rough set theory is a useful mathematical tool for dealing with the uncertainty and granularity in information systems. In this paper, rough approximations are introduced into quantales, a kind of partially ordered algebraic structure with an associative binary multiplication. The notions of (upper, lower) rough (prime, semi-prime, primary) ideals of quantales are proposed and verified to be the extended notions of usual (prime, semi-prime, primary) ideals of quantales respectively. Global order properties of all lower (upper) rough ideals of a quantale are also investigated. The rough radical of an upper rough ideal in a quantale is defined and the prime radical theorem of rings is generalized to upper rough ideals of quantales. Some results about homomorphic images of rough ideals and rough prime ideals of semigroups and rings are also generalized and improved in the field of quantales. © 2012 Elsevier Inc. All rights reserved. Source


Yuan J.,Yangzhou University
Jiaotong Yunshu Gongcheng Xuebao/Journal of Traffic and Transportation Engineering | Year: 2011

The high-temperature shear strength of asphalt mixture was confirmed by using uniaxial penetration test at 60°C. The 2D morphological characters of coarse aggregate were described in terms of shape, angularity and surface texture by using digital image processing technology. The gradation of coarse aggregate was identified based on equivalent ellipse method and sieve size correction factor. The morphological characters of coarse aggregate were extracted by MATLAB, and some characters affecting the high-temperature shear strength of asphalt mixture were proved. Euler number of image in digital topology was applied to quantify the skeleton stability of coarse aggregate. Analysis result shows that the shear strength of asphalt mixture increases due to coarse aggregates with low aspect ratio and eccentricity, high angularity index of equal ellipse perimeter and surface texture parameter. As Euler number decreases, the skeleton structure of coarse aggregate in asphalt mixture with dense framework structure becomes steadier, which leads to higher high-temperature shear strength of asphalt mixture. 9 tabs, 3 figs, 9 refs. Source


Time-delay Takagi-Sugeno fuzzy drive-response dynamical networks (TD-TSFDRDNs) are defined by extending the drive-response dynamical networks. Based on the LaSalle invariant principle, a simple and systematic adaptive control scheme is proposed to synchronize the TD-TSFDRDNs with a desired scalar factor. A sufficient condition for the generalized projective synchronization in TD-TSFDRDNs is derived. Moreover, numerical simulations are provided to verify the correctness and effectiveness of the scheme. © 2012 Chinese Physical Society and IOP Publishing Ltd. Source


Zhang X.,Nanjing Southeast University | Zhang X.,Yangzhou University | Tian Z.,Nanjing Southeast University | Yang S.-W.,Institute of High Performance Computing of Singapore | Wang J.,Nanjing Southeast University
Journal of Physical Chemistry C | Year: 2011

We systematically investigate the stability and electronic and magnetic properties of one-dimensional (1D) bimetallic organic sandwich molecular wires (BOSMWs), [CpTiCpTM]∞ (TM = Sc-Co, Cp = C5H 5), [CpCrCpTM]∞ (TM = V, Mn, Co), and [CpFeCpTM]∞ (TM = Cr, Co), using ab initio methods. All the BOSMWs are highly stable due to mixed ionic-covalent bonding. With the exceptions of [CpTiCpV]∞, [CpTiCpMn]∞, and [CpCrCpV]∞ exhibiting antiferromagnetic behavior, all the other BOSMWs are ferromagnetic with tunable magnetic moments. In particular, magnetic moments of [CpTiCpCo]∞ and [CpCrCpMn]∞ can be as high as 5 μB per unit cell. Our calculations further show that [CpTiCpTM]∞ (TM = Cr, Fe), [CpCrCpTM]∞ (TM = Fe, Co), and [CpFeCpCo]∞ are robust half-metals (HMs) with large HM gaps. Most importantly, we identify an empirical valence electron filling rule for these BOSMWs, and a BOSMW is found to be a half-metallic ferromagnet whenever N - 5(10) = 5(7) (N is the sum of the valence electrons of two metal atoms). This electron filling rule, together with the HM equations formulized in this study, can be extended to predict new HM BOSMWs. © 2011 American Chemical Society. Source


Wang J.-C.,CAS Shanghai Institutes for Biological Sciences | Xu H.,Zhejiang Academy of Agricultural Sciences | Zhu Y.,Zhejiang Academy of Agricultural Sciences | Liu Q.-Q.,Yangzhou University | Cai X.-L.,CAS Shanghai Institutes for Biological Sciences
Journal of Experimental Botany | Year: 2013

Starch composition and the amount in endosperm, both of which contribute dramatically to seed yield, cooking quality, and taste in cereals, are determined by a series of complex biochemical reactions. However, the mechanism regulating starch biosynthesis in cereal seeds is not well understood. This study showed that OsbZIP58, a bZIP transcription factor, is a key transcriptional regulator controlling starch synthesis in rice endosperm. OsbZIP58 was expressed mainly in endosperm during active starch synthesis. osbzip58 null mutants displayed abnormal seed morphology with altered starch accumulation in the white belly region and decreased amounts of total starch and amylose. Moreover, osbzip58 had a higher proportion of short chains and a lower proportion of intermediate chains of amylopectin. Furthermore, OsbZIP58 was shown to bind directly to the promoters of six starch-synthesizing genes, OsAGPL3, Wx, OsSSIIa, SBE1, OsBEIIb, and ISA2, and to regulate their expression. These findings indicate that OsbZIP58 functions as a key regulator of starch synthesis in rice seeds and provide new insights into seed quality control. © The Author [2013]. Source


Kang Y.,Yangzhou University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2010

OBJECTIVE: The interaction between plant growth promoting rhizobacteria (PGPR) and plants can be unstable, PGPR with PGP activities may be well adapted to particular soil environment. Based on this, we isolated and identified PGPRs from different rhizosphere soils according to their multiple mechanisms. METHODS: Preliminary screening of PGPRs under the premises of PGPR may having the abilities of N2-fixing, phosphate and potassium solubilization, and resistance against six common pathogenic fungi as well as rhizosphere colonization. After that, multiple PGP activities were detected in vitro. Finally, PGPRs were classified and identified by combining physiological and biochemical tests and 16S rRNA gene sequence analysis. RESULTS: Fourteen strains having various mechanisms of PGP activities such as NH3, IAA, HCN, siderophore, antibiotics production, phosphate and potassium solubilization, and N2-fixing were isolated from different rhizosphere soils in Yangzhou and Yancheng, Jiangsu province. These 14 isolates could be identified as Pseudomonas (7 isolates), Paenibacillus (3 isolates), Bacillus (2 isolates), Burkholderia (1 isolate) and Erwinia (1 isolate). CONCLUSION: Isolates with multiple PGP activities can also be rhizospheric competent, able to survive and colonize in the rhizosphere, providing promising isolates for PGPRs combination to resolve the challenges in field application of PGPR. Source


Wang B.,Yangzhou University
IET Information Security | Year: 2016

Hazay et al. initiated the formal study of leakage-resilient message authentication code (MAC) and presented a MAC scheme that is both leakage resilient and unforgeable against chosen message and no verification query attack (uf-cm-nvq). As the communication overhead of their construction is linear with the parameters that control the leakage bound, their scheme sacrifices efficiency in exchange for leakage resilience. In this study, the authors study the problem of designing leakage-resilient MACs in the public-key setting with acceptable communication efficiency. In particular, a notion called 'hidden identity weak hash proof system'(HID-wHPS) is introduced. Then a generic MAC construction is presented under the abstraction framework of HID-wHPS. Security properties guaranteed by HID-wHPS enable us to prove the author's construction to be both leakage resilient and uf-cm-nvq in a modular way. Finally, performance analysis shows that their MAC construction yields improved tagging-key size, tag size as well as computation overhead under the given leakage bound. © The Institution of Engineering and Technology 2016. Source


Dong Q.,Yangzhou University | Ding J.,University of Southern Mississippi
Computers and Mathematics with Applications | Year: 2016

Let A be a square matrix that is diagonalizable. We find all the commuting solutions of the quadratic matrix equation AXA=XAX, by taking advantage of the Jordan form structure of A, together with the help of a well-known theorem on the uniqueness of a solution to Sylvester's equation. Two special classes of the given matrix A are further investigated, including circular matrices and those that are equal to some of their powers. Moreover, all the non-commuting solutions are constructed when A is a Householder matrix, based on a spectral perturbation result. © 2016 Elsevier Ltd. Source


Tao Y.,Changzhou University | Gu X.,Changzhou University | Deng L.,Changzhou University | Qin Y.,Changzhou University | And 2 more authors.
Journal of Physical Chemistry C | Year: 2015

We report a Cu2+-modified β-cyclodextrin, Cu2-β-CD, that was self-assembled on poly(l-glutamic acid) (P-l-Glu) for chiral recognition of d-tryptophan (d-Trp). Cu2+ formed binuclear hydroxy-bridge (Cu(OH)2Cu) at the wider opening of the cavity of β-CD and acted as a cap to prevent high-energy water molecules from being released while forcing Trp isomers to enter through its narrower opening. Because H-bonds were favored to form between the high-energy water molecules and the amino groups of d-Trp inside the cavity of β-CD, d-Trp was thus recognized. The recognition capability of the Cu2-β-CD appeared to depend on the way the Cu2-β-CD self-assembled onto P-l-Glu, preferring via H-bonds rather than via electrostatic interactions, because electrostatic interactions between P-l-Glu and Cu(OH)2Cu could alter the configuration of the Cu2-β-CD cavity, resulting in weakened H-bonds between high-energy water molecules and D-Trp. © 2015 American Chemical Society. Source


Cui W.,Donghua University | Fang J.-A.,Donghua University | Zhang W.,Yangzhou University | Zhang W.,Hong Kong Polytechnic University | Wang X.,Donghua University
IET Control Theory and Applications | Year: 2014

In this study, the authors study the finite-time cluster synchronisation problem for a class of Markovian switching complex networks with stochastic noise perturbations. By constructing the suitable stochastic Lyapunov-Krasovskii functional, using finite-time stability theorem, inequality techniques and the properties of Weiner process, sufficient conditions are obtained to ensure finite-time cluster synchronisation for the complex networks with or without time delays. The effects of control parameters on cluster synchronisation speed and time delays are also analysed. Since finite-time cluster synchronisation means the optimality in convergence time and has better robustness and disturbance rejection properties, this study has important theory significance and practical application value. Finally, numerical examples are examined to illustrate the effectiveness of the analytical results. © The Institution of Engineering and Technology 2013. Source


Chen X.,Yangzhou University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2010

In order to obtain the antagonistic protein of Bacillus subtilis G87 and definitude its characterization. Methods of ammonium sulfate precipitating and column chromatography analyzing were used to isolate and purify the protein. A purified protein (peak 6-2-1) was obtained which molecular weight was 50.8 kD by SDS-PAGE and isoelectric point was 5.90 by IEF-PAGE. The antifungal protein contained 0.62% saccharide and some proline or hydroxyproline, but no lipid and aromatic amino acid. The inhibitory activity of the antifungal protein would decreased distinctly at the higher temperature (> or = 60 degrees C) and in the condition of alkalinity (pH > 8), but tolerant to ultraviolet radiation, chloroform, trypsin, proteinase K and pepsin. Antifungal protein of Bacillus subtilis G87 was a kind of glycoprotein without aromatic hydrocarbon. It was sensitive to higher temperature and tight alkalinity but not to proteinase analog and ultraviolet radiation et al. Source


Zhuang X.,Yangzhou University | Zhuang X.,University of Louisville | Xiang X.,University of Louisville | Grizzle W.,University of Alabama at Birmingham | And 10 more authors.
Molecular Therapy | Year: 2011

In this study, exosomes used to encapsulate curcumin (Exo-cur) or a signal transducer and activator of transcription 3 (Stat3) inhibitor, i.e., JSI124 (Exo-JSI124) were delivered noninvasively to microglia cells via an intranasal route. The results generated from three inflammation-mediated disease models, i.e., a lipopolysaccharide (LPS)-induced brain inflammation model, experimental autoimmune encephalitis and a GL26 brain tumor model, showed that mice treated intranasally with Exo-cur or Exo-JSI124 are protected from LPS-induced brain inflammation, the progression of myelin oligodendrocyte glycoprotein (MOG) peptide induced experimental autoimmune encephalomyelitis (EAE), and had significantly delayed brain tumor growth in the GL26 tumor model. Intranasal administration of Exo-cur or Exo-JSI124 led to rapid delivery of exosome encapsulated drug to the brain that was selectively taken up by microglial cells, and subsequently induced apoptosis of microglial cells. Our results demonstrate that this strategy may provide a noninvasive and novel therapeutic approach for treating brain inflammatory-related diseases. © 2011 The American Society of Gene & Cell Therapy. Source


Wang Z.,Donghua University | Wang Z.,Brunel University | Wang Y.,Donghua University | Liu Y.,Yangzhou University
IEEE Transactions on Neural Networks | Year: 2010

In this paper, the problem of stochastic synchronization analysis is investigated for a new array of coupled discretetime stochastic complex networks with randomly occurred nonlinearities (RONs) and time delays. The discrete-time complex networks under consideration are subject to: 1) stochastic nonlinearities that occur according to the Bernoulli distributed white noise sequences; 2) stochastic disturbances that enter the coupling term, the delayed coupling term as well as the overall network; and 3) time delays that include both the discrete and distributed ones. Note that the newly introduced RONs and the multiple stochastic disturbances can better reflect the dynamical behaviors of coupled complex networks whose information transmission process is affected by a noisy environment (e.g., internet-based control systems). By constructing a novel Lyapunov-like matrix functional, the idea of delay fractioning is applied to deal with the addressed synchronization analysis problem. By employing a combination of the linear matrix inequality (LMI) techniques, the free-weighting matrix method and stochastic analysis theories, several delay-dependent sufficient conditions are obtained which ensure the asymptotic synchronization in the mean square sense for the discrete-time stochastic complex networks with time delays. The criteria derived are characterized in terms of LMIs whose solution can be solved by utilizing the standard numerical software. A simulation example is presented to show the effectiveness and applicability of the proposed results. © 2009 IEEE. Source


Wang B.,Yangzhou University
IET Information Security | Year: 2010

In 2000, Yi et al. proposed two proxy multi-signature schemes which enable two or more original signers to delegate their signing power to a proxy signer. Combining proxy multi-signature with identity-based cryptography, Wang et al. proposed an identity-based proxy multi-signature scheme in 2007. Their scheme is claimed to be secure in the random oracle model. However, in this study, the author shows that Wang et al.'s scheme is vulnerable to a forgery attack described in this study. © 2010 The Institution of Engineering and Technology. Source


Fan L.,Yangzhou University
Journal of Thermal Analysis and Calorimetry | Year: 2010

The interaction of a flavonoid molecule (puerarin) with bovine serum albumin (BSA) was characterized by isothermal titration calorimetry (ITC), optical spectroscopic technique, and molecular modeling method under physiological conditions. The binding parameters for the reaction were calculated according to ITC experiments at different temperatures. The thermodynamic parameters, negative enthalpy changes (ΔH), and positive entropy (ΔS) indicated that the binding processes were entropically driven. The alterations of protein secondary structure in the presence of puerarin in aqueous solution were estimated by the evidences from FT-IR and CD spectroscopy with reductions of α-helices. On the basis of fluorescence resonance energy transfer (FRET) between excited tryptophan in BSA and BSA bound puerarin, the critical transfer distance and mean distance between tryptophan in BSA and puerarin were estimated. © 2010 Akadémiai Kiadó, Budapest, Hungary. Source


Liu Z.,Nanjing Southeast University | Chen H.,Nanjing Southeast University | Liu W.,Nanjing University of Information Science and Technology | Xu J.,Nanjing Southeast University | And 2 more authors.
Quantum Information Processing | Year: 2013

From the perspective of quantum circuit, a construction framework and a measurement framework of a general kind of four-qubit states are sketched, respectively. By utilizing the properties of this kind of states, a quantum secure direct communication (QSDC) protocol is put forward, which adopts the idea of optimal quantum superdense coding to achieve a maximal efficiency and high resources capacity. The security of the proposed protocol is discussed in detail and it is proved to be secure theoretically. Moreover, the sufficient and necessary condition of which multipartite states are suitable for optimal quantum superdense coding in quantum secure direct communication is figured out. © 2012 Springer Science+Business Media, LLC. Source


Zhang J.-H.,Lanzhou University of Technology | Li S.-R.,Yangzhou University
Mechanics of Advanced Materials and Structures | Year: 2013

The free vibration analysis of a functionally gradedmaterial (FGM)truncated conical shell is presented using themethod of generalized differential quadrature (GDQ). Based on Love's first approximation theory, governing equations are derived. The material properties ofFGMshells are assumed to be graded in the thickness direction according to a simple power law distribution in terms of the volume fractions of the constituents. Then, using the GDQ method, the natural frequencies of the shells for various boundary conditions can be obtained. The effects of the materials' constitution and the shape geometry on the natural frequencies are discussed in detail. Copyright © Taylor &Francis Group, LLC. Source


Li J.,Yangzhou University
American Journal of the Medical Sciences | Year: 2015

BACKGROUND:: CD163, a marker of M2 macrophages, possesses anti-inflammatory properties. This study aims to investigate the clinicopathological significance of CD163-positive macrophages in proliferative glomerulonephritis. METHODS:: Renal tissue samples from patients with lupus nephritis (LN, n = 22), antineutrophil cytoplasmic autoantibody (ANCA)-associated pauci-immune necrotizing glomerulonephritis (PNGN, n = 10), type 1 membranoproliferative glomerulonephritis (n = 5), minimal change disease (n = 8) and normal control kidneys (n = 3) were included in this study. The expression of CD163, CD68, CD20 and CD3 in renal tissues was detected by immunohistochemistry or immunofluorescence. The level of urinary neutrophil gelatinase-associated lipocalin (NGAL) was determined by enzyme-linked immunosorbent assay. RESULTS:: CD163 was mainly expressed in active crescentic glomerulonephritis, proliferative glomerular lesions and areas of tubulointerstitial injury. Patients with LN-IV and PNGN had numerous CD163-positive cells in glomerular and acute tubulointerstitial lesions. CD163-positive cells in glomeruli positively correlated to proteinuria yet negatively correlated to estimated glomerular filtration rate. There was a positive correlation between the number of CD163 cells in acute tubulointerstitial lesions and NGAL levels, whereas a negative correlation between CD163 numbers and estimated glomerular filtration rate. The number of CD163-positive cells in crescentic glomerulonephritis was more than other groups. In LN, the number of CD163 cells in the tubulointerstitial and glomerular lesions had a positive correlation with activity index. Dual staining showed that CD163-positive cells also expressed CD68, although they did not show any staining for CD20 or CD3. CONCLUSIONS:: CD163-positive macrophages were involved in the pathogenesis of proliferative glomerular lesions, active crescentic glomerulonephritis and acute tubular injury of patients with PNGN and active LN. © 2015 by the Southern Society for Clinical Investigation. Unauthorized reproduction of this article is prohibited. Source


Zong L.,Peoples Hospital of Jiangsu Province | Zong L.,University of Tokyo | Chen P.,Peoples Hospital of Jiangsu Province | Wang D.-X.,Yangzhou University
World Journal of Gastroenterology | Year: 2014

Aim: To evaluate human epidermal growth factor receptor 2 (HER2) and death decoy receptor (DcR3) as colorectal cancer prognostic indicators. Methods: Colorectal carcinoma specimens from 300 patients were analyzed by immunohistochemistry to detect the staining patterns of HER2 and DcR3. Classification of HER2 staining was carried out using the United States Food and Drug Administration semi-quantitative scoring system, with scores of 0 or 1+ indicating a tumor-negative (normal expression) status and scores of 2+ and 3+ indicating a tumor-positive (overexpression) status. Classification of DcR3 was carried out by quantitating the percentage of positive cells within the stained section, with < 10% indicating a tumor-negative status and ≥ 10% indicating a tumor-positive status. Correlation of the HER2 and DcR3 staining status with clinicopathological parameters [age, sex, tumor size, differentiation, and the tumor, node, metastasis (pTNM) classification] and survival was statistically assessed. Results: Tumor-positive status for HER2 and DcR3 was found in 18.33% and 58.33% of the 300 colorectal carcinoma specimens, respectively. HER2 tumorpositive status showed a significant correlation with tumor size (P = 0.003) but not with other clinicopathological parameters. DcR3 tumor-positive status showed a significant correlation with tumor differentiation (P < 0.001), pTNM stage (P < 0.001), and lymph node metastasis (P < 0.001). However, correlation coefficient analysis did not indicate that a statistically significant correlation exists between tumor-positive status for the HER2 and DcR3 overexpression (P = 0.236). Patients with specimens classified as DcR3-overexpressing had a significantly worse overall survival (OS) rate than those without DcR3 overexpression (median OS: 42.11 vs 61.21 mo; HR = 50.27, 95%CI: 44.90-55.64, P < 0.001). HER2 overexpression had no significant impact on median OS (35.10 mo vs 45.25 mo; HR = 44.40, 95%CI: 39.32-49.48, P = 0.344). However, patients with specimens classified as both HER2- and DcR3- overexpressing had a significantly poorer median OS than those with only HER2 overexpression (31.80 mo vs 52.20 mo; HR = 35.10, 95%CI: 22.04-48.16, P = 0.006). Conclusion: HER2 overexpression is not an independent prognostic marker of colorectal cancer, but DcR3 overexpression is highly correlated with lymph node metastasis and poor OS. © 2014 Baishideng Publishing Group Co., Limited. All rights reserved. Source


Pan Q.,Yangzhou University
Lecture Notes in Electrical Engineering | Year: 2013

Along with the social economy's development, security, environmental protection concept deepening, and requirements for emerging land redevelopment, sorting and recycling Survey and mapping technology is a new land development project, an important key link, which should increase the wealth of society, promote the protection of the ecological environment, and promote the social harmony, make sustainable development as the reference point, and make full use of Surveying and mapping technology for new land market development work to improve beneficial support. This paper summarizes the abundant use of surveying and mapping technology and work experience, uses the modern new surveying technology GPS real-time dynamic measurement system to measure and location the new land, and the establishment of management information system database. The surveying and mapping technology in emerging land market development projects play a superior effect and strong support. © 2013 Springer-Verlag Berlin Heidelberg. Source


Gong Z.,University of Wisconsin - Madison | Gong Z.,Yangzhou University | Wu Y.,University of Wisconsin - Madison | Koblizkova A.,Academy of Sciences of the Czech Republic | And 10 more authors.
Plant Cell | Year: 2012

Centromeres in most higher eukaryotes are composed of long arrays of satellite repeats. By contrast, most newly formed centromeres (neocentromeres) do not contain satellite repeats and instead include DNA sequences representative of the genome. An unknown question in centromere evolution is how satellite repeat-based centromeres evolve fromneocentromeres. We conducted a genome-wide characterization of sequences associated with CENH3 nucleosomes in potato (Solanum tuberosum). Five potato centromeres (Cen4, Cen6, Cen10, Cen11, and Cen12) consisted primarily of single- or low-copy DNA sequences. No satellite repeats were identified in these five centromeres. At least one transcribed gene was associated with CENH3 nucleosomes. Thus, these five centromeres structurally resemble neocentromeres. By contrast, six potato centromeres (Cen1, Cen2, Cen3, Cen5, Cen7, and Cen8) contained megabase-sized satellite repeat arrays that are unique to individual centromeres. The satellite repeat arrays likely span the entire functional cores of these six centromeres. At least four of the centromeric repeats were amplified from retrotransposon-related sequences and were not detected in Solanum species closely related to potato. The presence of two distinct types of centromeres, coupled with the boom-and-bust cycles of centromeric satellite repeats in Solanum species, suggests that repeat-based centromeres can rapidly evolve from neocentromeres by de novo amplification and insertion of satellite repeats in the CENH3 domains. © 2012 American Society of Plant Biologists. All rights reserved. Source


Song H.,Nanjing Agricultural University | Yin Z.,Yangzhou University | Chao M.,Nanjing Agricultural University | Ning L.,Nanjing Agricultural University | And 2 more authors.
Plant, Cell and Environment | Year: 2014

In this paper, we analyzed the function and modulation of the soybean phosphate transporter gene GmPT1. Overexpression of GmPT1 in tobacco showed that this gene played a role in Pi remobilization. In a soybean RIL population, the expression level of GmPT1 showed significantly positive correlations with seed yield, phosphorus-use efficiency, photosynthetic rate (PN) and the quantum yield of PSII primary photochemistry (ΦPSII). Expression quantitative trait loci (eQTL) mapping for GmPT1 revealed one cis-eQTL and one trans-eQTL. These results suggest the possibility to improve soybean seed yields under Pi-limiting conditions by modulating GmPT1 expression. Phosphate (Pi) remobilization within a plant is critical for plant survival under Pi-limiting conditions. In this paper, a soybean Pi transporter gene, GmPT1, was characterized. A marked induction of GmPT1 transcript was observed in young leaves, mature leaves and lateral roots during long-term Pi starvation. Transgenic tobacco plants containing the GmPT1 gene were obtained using an Agrobacterium-mediated transformation system. Compared with wild-type plants, transgenic plants showed significant increases in phosphorus-use efficiency (PUE), photosystem II (PSII) function, total dry weight and seed weight under Pi-deficient conditions. GmPT1 expression levels and PUE were determined in a soybean recombinant inbred line population during a pot experiment that was conducted to measure chlorophyll fluorescence parameters, photosynthetic rate (PN) and seed yield. Correlation analysis revealed that GmPT1 expression levels had significantly positive correlations with seed yield, PUE, PN and the quantum yield of PSII primary photochemistry (ΦPSII). Expression quantitative trait loci (eQTL) mapping for GmPT1 revealed two eQTLs, one of which coincided with both the physical location of GmPT1 and a QTL associated with seed yield. These results suggest that GmPT1 plays a role in Pi remobilization, and it may be possible to improve soybean seed yields under Pi-limiting conditions by modulating GmPT1 expression levels. © 2013 John Wiley & Sons Ltd. Source


Yin Y.,Yangzhou University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2013

Positive regulatory factor A (PrfA) protein plays a key role in the pathogenicity of Listeria monocytogenes by regulating the expression of virulence genes. We studied the regulation functions of PrfA and its role in Listeria monocytogenes (Lm) virulence. Extracellular proteins were obtained from the supernatants of parental strain LM4 and mutant strain LM4deltaprfA cultured in minimal medium. We used two-dimensional gel electrophoresis and matrix associated laser dissociation/ionization time of flight mass spectrometry (MALDI- TOF-MS) to analyze the differences of secreted proteins between LM4 and LM4deltaprfA. The electrophoresis results show that 31 different spots, 19 spots corresponding 12 proteins were identified by MALDI- TOF-MS. Some virulence related proteins were verified, such as InlC, ActA and LLO. Some new proteins that are regulated by PrfA include D-alanyl-D-alanine carboxypeptidase, dipeptide Glycine and Trytophan (GW) repeat-containing surface protein, transcriptional regulator and some hypothetical proteins with unknown functions. Real-time quantitative PCR was conducted to verify the proteomics results. The mRNA expression level of hly, actA and inlC gene was significantly reduced, and that of D-alanyl-D-alanine carboxypeptidase and GW repeat-containing surface protein's synthesis also had a reduction in LM4deltaprfA strain. PrfA plays key roles on the regulation of genes in LIPI- I and LIPI- II. Source


Jin Z.,Yangzhou University | Cui Z.,Shanghai JiaoTong University
Computational Materials Science | Year: 2012

To predict and to control the microstructural evolution during dynamic recrystallization (DRX), a modified cellular automaton (CA) model based on mathematical statistics theory and physical metallurgical principles is developed. Initial microstructure and thermo-mechanical parameters are used as input data to the CA model. Dislocation density is used as a crucial internal state variable to link microstructural evolution with macroscopic flow stress. The latter two are output data, which can be compared with experimental one. In order to exhibit the effect of deformation stored energy on DRX, both the nucleation rate and the growth velocity of each recrystallizing grain (R-grain) are calculated from the dislocation density. The growth kinetics of R-grain is calculated from the metallurgical principles, and the nucleation kinetics is evaluated from a statistically based dislocation-related nucleation model. Model parameters are identified by a flow stress-based inverse analysis method, and then their variations with thermo-mechanical parameters (strain rate and temperature) are estimated and integrated into the CA model. The good agreement between the simulations and the experiments demonstrates the availability and predictability of the modified CA model. © 2012 Elsevier B.V. All rights reserved. Source


Wei Z.,Nanjing Agricultural University | Yang X.,Nanjing Agricultural University | Yin S.,Yangzhou University | Shen Q.,Nanjing Agricultural University | And 2 more authors.
Applied Soil Ecology | Year: 2011

Ralstonia solanacearum (Smith) has been a severe pathogen on tomato worldwide and no effective control measure is available yet. To develop a new bio-product for controlling this disease, antagonistic Bacillus amyloliquefaciens strains QL-5 and QL-18 were isolated from rhizosphere soil of tomato and used separately or together to fortify organic fertilisers to control bacterial wilt of tomato (BWT), both in the greenhouse and in the field. The bio-organic fertiliser (BOF) fortified with a mixed inoculation of QL-5 and QL-18 effectively decreased R. solanacearum disease incidence in both greenhouse and field conditions. However, the suppressive efficacy in the field depended on the season. Disease incidence in the spring crop season in 2009 and 2010 was significantly reduced, while a low suppressive effect was observed in the autumn crop season in 2008 and 2009. The initial R. solanacearum population in the soil was nearly three times lower in the spring before transplanting seedlings than in the autumn crop season. The pathogen population in the field was positively and significantly correlated with air temperature but was not correlated with relative humidity. The interaction between BOF and other soil and climatic factors that affect pathogen density in the field should be clarified. © 2011 Elsevier B.V. Source


Guo P.,CAS Shanghai Institute of Applied Physics | Guo P.,University of Chinese Academy of Sciences | Tu Y.,Yangzhou University | Yang J.,CAS Shanghai Institute of Applied Physics | And 4 more authors.
Physical Review Letters | Year: 2015

By combining molecular dynamics simulations and quantum mechanics calculations, we show the formation of a composite structure composed of embedded water molecules and the COOH matrix on carboxyl-terminated self-assembled monolayers (COOH SAMs) with appropriate packing densities. This composite structure with an integrated hydrogen bond network inside reduces the hydrogen bonds with the water above. This explains the seeming contradiction on the stability of the surface water on COOH SAMs observed in experiments. The existence of the composite structure at appropriate packing densities results in the two-step distribution of contact angles of water droplets on COOH SAMs, around 0° and 35°, which compares favorably to the experimental measurements of contact angles collected from forty research articles over the past 25 years. These findings provide a molecular-level understanding of water on surfaces (including surfaces on biomolecules) with hydrophilic functional groups. © 2015 American Physical Society. © 2015 American Physical Society. Source


Zhang F.,Yangzhou University
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2013

To summarize the recent research situation and progress of decellularized matrix in tissue engineered trachea transplantation and to forecast the possible perspects. Recent original articles about study and application for decellularized matrix in tissue engineered trachea were reviewed. The application and study of different decellularized matrices involved in animals or patients with tracheal lesions were elaborated. Decellularized matrices researched and applied in tissue engineered trachea include jejunum, urinary bladder, aorta, and trachea. Decellularized urinary bladder matrix and jejunal matrix appears to be efficacious method for the patch repair of partial circumferential tracheal defects. The application of decellularized aortic matrix may need more study, and decellularized tracheal matrix has a bright future in long tracheal defects. Source


Yang X.,Chongqing Jiaotong University | Song Q.,Chongqing Jiaotong University | Liu Y.,Yangzhou University | Liu Y.,King Abdulaziz University | Zhao Z.,Huzhou Teachers College
Neurocomputing | Year: 2015

Stability analysis of fractional-order neural networks with delay is addressed in this paper. By using the contracting mapping principle, method of iteration and inequality techniques, a sufficient condition is established to ensure the existence, uniqueness and finite-time stability of the equilibrium point of the proposed networks. Finally, based on the Predictor-Corrector Approach, two numerical examples are presented to illustrate the validity and feasibility of the obtained result. © 2014 Elsevier B.V. Source


Hao D.,Nanjing Agricultural University | Hao D.,Jiangsu Yanjiang Institute of Agricultural science | Chao M.,Nanjing Agricultural University | Yin Z.,Yangzhou University | Yu D.,Nanjing Agricultural University
Euphytica | Year: 2012

Chlorophyll fluorescence parameters are generally used to characterize the intrinsic action of photosystem II (PSII), which is interrelated with the photosynthetic capacity. Mapping of quantitative trait loci for chlorophyll fluorescence parameters and associated traits is important for genetic improvement in soybean. In this study, a genome-wide association analysis was conducted to detect key single-nucleotide polymorphisms (SNPs) associated with chlorophyll content (chl) and chlorophyll fluorescence using 1,536 SNPs in a soybean landraces panel. The analysis revealed significant correlations among chl and five chlorophyll fluorescence parameters, including maximum quantum yield of PSII primary photochemistry in the dark-adapted state (Fv/Fm), light energy absorbed per reaction center (ABS/RC), quantum yield for electron transport (ETo/ABS), probability that a trapped exciton moves an electron into the electron transport chain beyond QA - (ETo/TRo), and performance index on absorption basis (PI ABS). Genome-wide association analysis using a mixed linear model detected 51 SNPs associated with chl and chlorophyll fluorescence parameters. Among these identified SNPs, 14 SNPs were co-associated with two or more different traits in this study, and 8 SNPs were co-associated with soybean yield and yield components in our previous study. These significant SNPs will help to better understand the genetic basis of photosynthesis-related physiological traits, and facilitate the pyramiding of favorable alleles for photosynthetic traits in soybean marker assisted selection schemes for high photosynthetic efficiency. © 2012 Springer Science+Business Media B.V. Source


Wang J.,Nanjing University of Information Science and Technology | Zhang Z.,Nanjing University of Information Science and Technology | Li B.,Yangzhou University | Lee S.,Kyung Hee University | Sherratt R.,University of Reading
IEEE Transactions on Consumer Electronics | Year: 2014

Various fall-detection solutions have been previously proposed to create a reliable surveillance system for elderly people with high requirements on accuracy, sensitivity and specificity. In this paper, an enhanced fall detection system is proposed for elderly person monitoring that is based on smart sensors worn on the body and operating through consumer home networks. With treble thresholds, accidental falls can be detected in the home healthcare environment. By utilizing information gathered from an accelerometer, cardiotachometer and smart sensors, the impacts of falls can be logged and distinguished from normal daily activities. The proposed system has been deployed in a prototype system as detailed in this paper. From a test group of 30 healthy participants, it was found that the proposed fall detection system can achieve a high detection accuracy of 97.5%, while the sensitivity and specificity are 96.8% and 98.1% respectively. Therefore, this system can reliably be developed and deployed into a consumer product for use as an elderly person monitoring device with high accuracy and a low false positive rate. © 1975-2011 IEEE. Source


Liu Y.,Yangzhou University | Ho D.W.C.,City University of Hong Kong | Wang Z.,Brunel University
International Journal of Control | Year: 2012

In this article, the distributed consensus problem is considered for discrete-time delayed networks of dynamic agents with fixed topologies, where the networks under investigation are directed and the time delays involved are distributed time delays including a single or multiple time delay(s) as special cases. By using the invariance principle of delay difference systems, a new unified framework is established to deal with the consensus for the discrete-time delayed multi-agent system. It is shown that the addressed discrete-time network with arbitrary distributed time delays reaches consensus provided that it is strongly connected. A numerical example is presented to illustrate the proposed methods. © 2012 Taylor & Francis Group, LLC. Source


Ye G.,Yangzhou University
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2013

To review the recent research progress of the bioreactor biophysical factors in cartilage tissue engineering. The related literature concerning the biophysical factors of bioreactor in cartilage tissue engineering was reviewed, analyzed, and summarized. Oxygen concentration, hydrostatic pressure, compressive force, and shear load in the bioreactor system have no unified standard parameters. Hydrostatic pressure and shear load have been in controversy, which restricts the application of bioreactors. The biophysical factors of broreactor in cartilage tissue engineering have to be studied deeply. Source


Xia P.,Yangzhou University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2013

It is difficult to stimulate efficient gut mucosal immune response to intestinal infection. This article critically reviews the research progressin Escherichia coli strain Nisslel917 ( EcN) actingas a safe vehicle for the intestinal mucosal immunity, to restore gastrointestinal disorder and relieve ulcerative colitis. EcN is an orally administered probiotics, combining the excellent colonization and non-immunogenic character, and can be an ideal live vector candidate. This strain could be a tumor-targeted delivery of TAT-Apoptin fusion gene to colorectal cancer. In the treatment of ulcerative colitis and Crohn's disease, the recombinant strain of EcN can be used as a target therapeutics for defensins presenting. Genetically modified EcN could be an ideal carrier organism for gut-focused in situ synthesis and expression of specific localized antigen delivery into the intestine, and stimulate specific mucosal immune response. In vitro trial demonstrated that intestinal recombinant E. coli Nissle-HA110-120 has the potential to stimulate antigen specific response, but EcN itself does not induce mucosal immune response and influence peripheral tolerance to self-antigen. At the same time, there are evidences that EcN is safe. Recombinant E. coli Nissle-HA110-120 does not migrate, clonally expand and activate specific CD4+ T cells, neither in healthy mice nor in other animals with acute colitis, even when the intestinal epithelium suffer from inflammation and the barrier function of the epithelial layer being destroyed. Source


Guo L.,Beihang University | Cao S.,Beihang University | Cao S.,Yangzhou University
ISA Transactions | Year: 2014

The problem of anti-disturbance control has been an eternal topic along with the development of the control theory. However, most methodologies can only deal with systems subject to a single equivalent disturbance which was merged by various types of uncertainties. In this paper, a review on anti-disturbance control is presented for systems with multiple disturbances. First, the classical control methods are briefly reviewed for disturbance attenuation or rejection problems. Then, recent advances in disturbance observer based control (DOBC) theory are introduced and especially, the composite hierarchical anti-disturbance control (CHADC) is firstly addressed. A comparison of different approaches is briefly carried out. Finally, focuses in the field on the current research are also addressed with emphasis on the practical application of the techniques. © 2013 ISA. Source


Liu Y.,Yangzhou University | Wang Z.,Brunel University | Liang J.,Nanjing Southeast University | Liu X.,Brunel University
IEEE Transactions on Cybernetics | Year: 2013

In this paper, the synchronization problem is studied for an array of N identical delayed neutral-type neural networks with Markovian jumping parameters. The coupled networks involve both the mode-dependent discrete-time delays and the mode-dependent unbounded distributed time delays. All the network parameters including the coupling matrix are also dependent on the Markovian jumping mode. By introducing novel Lyapunov-Krasovskii functionals and using some analytical techniques, sufficient conditions are derived to guarantee that the coupled networks are asymptotically synchronized in mean square. The derived sufficient conditions are closely related with the discrete-time delays, the distributed time delays, the mode transition probability, and the coupling structure of the networks. The obtained criteria are given in terms of matrix inequalities that can be efficiently solved by employing the semidefinite program method. Numerical simulations are presented to further demonstrate the effectiveness of the proposed approach. © 2012 IEEE. Source


Wang J.,Henan University of Science and Technology | Zhu H.,Henan University of Science and Technology | Yang Z.,Henan University of Science and Technology | Liu Z.,Yangzhou University
Indian Journal of Pharmacology | Year: 2013

Objectives: Oxidative stress with subsequent lipid peroxidation (LP) has been suggested as a mechanism for lead-induced toxicity. The current study was carried out to evaluate antioxidant activity of hesperetin against lead acetate-induced oxidative stress. Materials and Methods: The male rats were treated with hesperetin in combination with lead acetate (500 mg/L). Results: The results indicated that hesperetin alone did not induce any significant changes in the biochemistry of serum, liver, and kidney tissues. On the other hand, lead-induced oxidative stress as indicated by significant changes in serum biochemical parameters, including increased lipid peroxide and decreased reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) levels in liver and kidney tissues. Hesperetin succeeded in improving these biochemical parameters towards the normal values of control. Conclusions: It suggests that hesperetin shows antioxidant activity and plays a protective role against lead-induced oxidative damage in liver and kidney of rats. Source


Jin Z.,Shanghai JiaoTong University | Jin Z.,Yangzhou University | Cui Z.,Shanghai JiaoTong University
Materials Science and Engineering A | Year: 2010

Since it is difficult to accurately describe the nucleation behavior through analytical calculation or experimental observation, an inverse analysis method is used to evaluate the reasonability of two different nucleation models, i.e., a conventional constant nucleation model where the nucleation rate is assumed to be independent of strain and a dislocation-related nucleation model where it is strain dependent. In order to accurately estimate the parameters in the above two models, a flow stress-based inverse analysis method is developed. This method is implemented by coupling a cellular automata (CA) model with an adaptive response surface method (ARSM). Then the reasonability of each nucleation model is evaluated by comparing the level of agreement between the simulations and the experiments. Taking a low carbon steel deformed at 1173K and 0.1s-1 as an example, it is shown that the flow stress-based inverse analysis method is able to estimate the nucleation parameters accurately and the introduction of the dislocation-related nucleation model makes the simulation even closer to the actual physical process. © 2010 Elsevier B.V. Source


Ye X.-S.,Yangzhou University
Journal of Applied Physics | Year: 2014

We explore the structure of the energy spectra of quasi-one dimensional organic conductors subjected to the field-induced spin-density-wave (FISDW) state. We show that the structure of the energy spectra can exhibit Hofstadter butterfly, which is generally believed to appear in two dimensional systems. The phenomenon of the quantized Hall conductance due to FISDW is also investigated. We find that the Hall number L, which is defined by L = σxy/(e2/h), coincides with the number described by FISDW order parameter. The sign reversal of the quantized Hall conductance is discussed theoretically. © 2014 AIP Publishing LLC. Source


Yu C.-M.,Yuan Ze University | Chen C.-Y.,National Ilan University | Chao H.-C.,Yangzhou University
IEEE Network | Year: 2015

Cloud storage such as Dropbox and Bitcasa is one of the most popular cloud services. Currently, with the prevalence of mobile cloud computing, users can even collaboratively edit the newest version of documents and synchronize the newest files on their smart mobile devices. A remarkable feature of current cloud storage is its virtually infinite storage. To support unlimited storage, the cloud storage provider uses data deduplication techniques to reduce the data to be stored and therefore reduce the storage expense. Moreover, the use of data deduplication also helps significantly reduce the need for bandwidth and therefore improve the user experience. Nevertheless, in spite of the above benefits, data deduplication has its inherent security weaknesses. Among them, the most severe is that the adversary may have an unauthorized file downloading via the file hash only. In this article we first review the previous solutions and identify their performance weaknesses. Then we propose an alternative design that achieves cloud server efficiency and especially mobile device efficiency. © 1986-2012 IEEE. Source


Fei K.,Yangzhou University
Engineering Geology | Year: 2016

Clay-aggregate mixtures are widely distributed in nature and are often used in engineering applications. To improve the understanding of the effects of coarse particles on the mechanical behavior of clay-aggregate mixtures, a series of oedometer and triaxial tests were conducted on clay-steel bead and clay-fine gravel mixtures. Based on computed tomography scans, both the number and total volume of the inter-particle voids were observed to increase with the coarse aggregate content. Because of the combined effects of the inter-particle voids and clay-aggregate skeleton, the compression index of the mixture first increased and then decreased with the coarse aggregate content. During the undrained loading, the specimen with higher compressibility tended to generate a larger pore-water pressure and exhibit a smaller shear strength. The effective friction angle was observed to increase with the coarse aggregate content. For the clay specimens mixed with 33.3% 1-mm beads, 2-mm beads, and fine gravels, the increases in the effective friction angles were 4.4°, 3.4°, and 6.2°, respectively. © 2016 Elsevier B.V. Source


Chen H.,Wenzhou University | Dai W.,Wenzhou University | Chen Y.,Wenzhou University | Xu Q.,Wenzhou University | And 7 more authors.
Green Chemistry | Year: 2014

Unexpected dimethylsulfinyl anions (I), generated in situ from the superbase system CsOH-DMSO, was found to be a highly active catalyst for controllable nitrile hydration reactions in water, which selectively afforded the versatile amides via interesting Cs-activated I-catalyzed direct and indirect hydration mechanisms involving an O-transfer process from DMSO onto the nitriles. © the Partner Organisations 2014. Source


Li Y.,Northwestern Polytechnical University | Li Y.,Yangzhou University | Huang Y.,Northwestern Polytechnical University | Qi S.,Northwestern Polytechnical University | And 3 more authors.
Applied Surface Science | Year: 2012

Strontium ferrite particles were firstly prepared by sol-gel method and self-propagating synthesis, and then the polyaniline/strontium ferrite/multiwalled carbon nanotubes composites were synthesized through in situ polymerization approach. Structure, morphology and properties of the composite were characterized by various instruments. XRD analysis shows that the output of PANI increases with the increase of the content of MWCNTs, due to the large surface area of MWCNTs. Because of the coating of PANI, the outer diameter of MWCNTs increases from 10 nm to 20-40 nm. The electrical conductivity of the composites increases with the amount increase of MWCNTs and reaches 7.2196 S/cm in the presence of 2 g MWCNTs. The coercive force of the composites prepared with 2 g MWCNTs is 7457.17 Oe, which is much bigger than that of SrFe 12O 19 particles 6145.6 Oe, however, both the saturation magnetization and the remanent magnetization of the composite become much smaller than those of SrFe 12O 19 particles. The electromagnetic properties of the composite are excellent in the frequency range of 2-18 GHz, which mainly depend on the dielectric loss in the range of 2-9 GHz, and mainly on the magnetic loss in the range of 9-18 GHz. © 2011 Published by Elsevier B.V. All rights reserved. Source


Jin W.,Yangzhou University
Brazilian Journal of Physics | Year: 2014

For an initial uniform superposition over all possible computational basis states, we explore the performance of Grover's search algorithm geometrically when imposing a perturbation on the Walsh-Hadamard transformation contained in the Grover iteration. We give the geometric picture to visualize the quantum search process in the three-dimensional space and show that Grover's search algorithm can work well with an appropriately chosen perturbation. Thereby we corroborate Grover's conclusion that if the perturbation is small, then it will have little impact of an impact on the implementation of this algorithm. We also prove that Grover's path cannot achieve a geodesic under a perturbation of the Fubini-Study metric. © 2014 Sociedade Brasileira de Física. Source


Wang X.,Changshu Institute of Technology | Wang X.,Yangzhou University | Gu Z.,Changshu Institute of Technology | Jiang H.,Virginia Polytechnic Institute and State University
Animal | Year: 2013

MicroRNAs (miRNAs) are a class of ∼22 nucleotide-long small noncoding RNAs that target mRNAs for translational repression or degradation. miRNAs target mRNAs by base-pairing with the 3′-untranslated regions (3′-UTRs) of mRNAs. miRNAs are present in various species, from animals to plants. In this review, we summarize the identification, expression, and function of miRNAs in four important farm animal species: cattle, chicken, pig and sheep. In each of these species, hundreds of miRNAs have been identified through homology search, small RNA cloning and next generation sequencing. Real-time RT-PCR and microarray experiments reveal that many miRNAs are expressed in a tissue-specific or spatiotemporal-specific manner in farm animals. Limited functional studies suggest that miRNAs have important roles in muscle development and hypertrophy, adipose tissue growth, oocyte maturation and early embryonic development in farm animals. Increasing evidence suggests that single-nucleotide polymorphisms in miRNA target sites or miRNA gene promoters may contribute to variation in production or health traits in farm animals. Copyright © The Animal Consortium 2013. Source


Wang B.,Yangzhou University
International Review on Computers and Software | Year: 2012

Wu et al. defined three security notions for server aided verification (SAV) protocols to prevent a server from convincing a verifier that an invalid signature is valid. Security against strong collusion attacks provides the strongest security guarantee among these notions. They constructed SAV protocols to meet these notions respectively. But they did not provide concrete running time to show that the running time of a verifier in their SAV protocol is strictly less than that of a verifier in the original verification protocol. In addition, a problem left open by their work is to design a SAV protocol which is unforgeable without random oracles as well as sound against strong collusion attacks. To address the above issues, we first design a SAV-Hofheinz protocol for a short signature proposed by Hofheinz, which is unforgeable in the standard model. Then we implement SAV-Hofheinz by the PBC library and show that the running time of a verifier in SAV-Hofheinz is strictly less than that of a verifier in the original verification protocol. © 2012 Praise Worthy Prize S.r.l. Source


Pan J.,Yangzhou University
Wuli Xuebao/Acta Physica Sinica | Year: 2013

According to density functional theory of first-principles calculations theory, we systematically study the electronic structures, magnetisms and stabilities of transition metal (TM = V, Cr, Mn) doped MoS2 single-layers. The results show that V- and Mn- monodoped systems each have magnetism and the magnetic moment mainly concentrates on the transition metal dopant atom, but Cr-doped MoS2 does not display magnetism. Further study on the magnetic coupling of double atoms doped MoS2 shows that the stable ferromagnetic state at room temperature is observed in the Mn-doped MoS2. However, the system shows a non-spin polarization state due to doping with V. The calculated formation energy indicates that the Mn-doped MoS2 is the most stable system. Therefore, Mn-doped single-layer MoS2 maybe have potential applications in the spin electronic devices due to its good ferromagnetism and reliable stability. © 2013 Chinese Physical Society. Source


Tian C.,Yancheng Institute of Technology | Zhang L.,Technical University of Denmark | Lin Z.,Yangzhou University
Journal of the Franklin Institute | Year: 2011

In this paper, we develop a theoretical framework for investigating spatial patterns on plankton allelopathy with cross-diffusion. We show that under some conditions the cross-diffusion is able to induce the Turing instability, which is further confirmed by the numerical simulations. Moreover, applying the LeraySchauder degree theory, we demonstrate that the cross-diffusion leads to an inhomogeneous stationary pattern provided with even stronger conditions. Finally, the wavenumber and the type of pattern selection are computed numerically. Our theoretical results of the spatial pattern are coincident with the experimental observations. © 2010 The Franklin Institute. Published by Elsevier Ltd. All rights reserved. Source


Chu H.J.,Los Alamos National Laboratory | Chu H.J.,Yangzhou University | Wang J.,Los Alamos National Laboratory | Beyerlein I.J.,Los Alamos National Laboratory | Pan E.,University of Akron
International Journal of Plasticity | Year: 2013

When a lattice glide dislocation approaches a bi-metal interface with relatively low shear strength, it causes the interface to shear. Interfacial shearing is accommodated by the nucleation and growth of interfacial dislocations, which have an attractive interaction with the incoming dislocation. Thus a critical length scale exists at which the net force on the incoming lattice glide dislocation can transition from being initially repulsive to attractive. In this paper, we develop dislocation-based interface shear models in order to represent this mechanism of interface/dislocation interaction at the continuum scale. Three versions are devised with different degrees of complexity and hence computational cost: the continuous shear model (CSM), simplified-CSM model (SCSM), and single dislocation shear model (SDSM). We simulate the interaction processes with these three models by means of a Green's function method for an anisotropic bimaterial. All three models find that the critical length scale at which the dislocation becomes attracted to the interface increases as the interfacial shear resistance decreases. While the most complex model of the three, the CSM, performs the best, the SCSM and SDSM are more advantageous for implementation into higher-length scale dislocation dynamics models. © 2012 Elsevier Ltd. All rights reserved. Source


Shen J.,Yangzhou University | Wang W.,Nanjing Southeast University
Tumu Gongcheng Xuebao/China Civil Engineering Journal | Year: 2011

In order to completely measure the complexity of the spatial layout and orderliness of urban intersections, a complexity degree model was developed from macro and micro perspectives. Conflict point and conflict probability were selected as the two key factors, one of which was analyzed using the diagram method and photogrammetry principle, and the other determined by using statistical analysis and curve fitting. The complexity degree values of intersections of different spatial layout under various traffic demands were calculated. The results indicate that the complexity degree values of intersections of the same type increase with volume and those of intersections of different types increase with the number of lanes. Complexity degree is a comprehensive quantitative index showing the working condition of intersections, and can be applied to planning urban road networks and safety appraisal of intersections. Source


Qian Q.,Nanjing University of Aeronautics and Astronautics | Chen S.,Nanjing University of Aeronautics and Astronautics | Zhou X.,Yangzhou University
Knowledge-Based Systems | Year: 2013

Side information, like must-link (ML) and cannot-link (CL), has been widely used in single-view classification tasks. However, so far such information has never been applied in multi-view classification tasks. In many real world situations, data with multiple representations or views are frequently encountered, and most proposed algorithms for such learning situations require that all the multi-view data should be paired. Yet this requirement is difficult to satisfy in some settings and the multi-view data could be totally unpaired. In this paper, we propose an learning framework to design the multi-view classifiers by only employing the weak side information of cross-view must-links (CvML) and cross-view cannot-links (CvCL). The CvML and the CvCL generalize the traditional single-view must-link (SvML) and single-view cannot-link (SvCL), and to the best of our knowledge, are first definitely introduced and applied into the multi-view classification situations. Finally, we demonstrate the effectiveness of our method in our experiments. © 2013 Elsevier B.V. All rights reserved. Source


Wang Y.X.,Yangzhou University | Wang Y.X.,Leibniz University of Hanover | Specht A.,Leibniz University of Hanover | Horst W.J.,Leibniz University of Hanover
New Phytologist | Year: 2011

Zinc (Zn) deficiency has been recognized as a potential risk for human health in many developing regions where staple food with low micronutrient density represents a major proportion of the diet. The success of strategies to increase Zn content in the edible part of crops requires better understanding of Zn transport to, and distribution within, the grains. The transfer of Zn from the growth medium to wheat (Triticum aestivum) grains in an ear culture system was investigated by using the stable Zn isotope 70Zn, and the spatial distribution of Zn within the grains was studied by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Zinc was readily transported in the stem up to the rachis. More Zn accumulated in the stem when higher amounts of Zn were supplied to the medium. Once Zn was transported into the grain, Zn accumulated particularly in the crease vascular tissue. The gradient of 70Zn concentration between crease vascular tissue, aleurone layer and endosperm demonstrates that Zn is distributed within grain through the crease phloem. These results suggest that two barriers of Zn transport into wheat grains may exist: between the stem tissue rachis and the grain, and the maternal and filial tissues in the grain. © The Authors (2010). Journal compilation © New Phytologist Trust (2010). Source


Zhu H.H.,Yangzhou University
World journal of gastroenterology : WJG | Year: 2012

To determine if serum inter-cellular adhesion molecule 1 (ICAM-1) is an early marker of the diagnosis and prediction of severe acute pancreatitis (SAP) within 24 h of onset of pain, and to compare the sensitivity, specificity and prognostic value of this test with those of acute physiology and chronic health evaluation (APACHE) II score and interleukin-6 (IL-6). Patients with acute pancreatitis (AP) were divided into two groups according to the Ranson's criteria: mild acute pancreatitis (MAP) group and SAP group. Serum ICAM-1, APACHE II and IL-6 levels were detected in all the patients. The sensitivity, specificity and prognostic value of the ICAM-1, APACHE II score and IL-6 were evaluated. The ICAM-1 level in 36 patients with SAP within 24 h of onset of pain was increased and was significantly higher than that in the 50 patients with MAP and the 15 healthy volunteers (P < 0.01). The ICAM-1 level (25 ng/mL) was chosen as the optimum cutoff to distinguish SAP from MAP, and the sensitivity, specificity, positive predictive value, negative predictive value (NPV), positive likelihood ratio and negative likelihood ratio were 61.11%, 71.42%, 0.6111, 0.7142, 2.1382 and 0.5445, respectively. The area under the curve demonstrated that the prognostic accuracy of ICAM-1 (0.712) was similar to the APACHE-II scoring system (0.770) and superior to IL-6 (0.508) in distinguishing SAP from MAP. ICAM-1 test is a simple, rapid and reliable method in clinical practice. It is an early marker of diagnosis and prediction of SAP within the first 24 h after onset of pain or on admission. As it has a relatively low NPV and does not allow it to be a stand-alone test for the diagnosis of AP, other conventional diagnostic tests are required. Source


Liu C.,Yangzhou University
Nongye Jixie Xuebao/Transactions of the Chinese Society for Agricultural Machinery | Year: 2015

The low head pump system, mainly axial-flow pump system research and technological innovation in China are introduced. The research and development trend on hydraulic performance of the axial flow pump system are discussed. The applications of high speed axial-flow pump hydraulic model and development progress are presented. Through inductive characteristics of axial-flow pump system, the classification of the system is put forward according to the installation position of the motor, namely the shaft extension type axial-flow pump system and the tubular axial-flow pump system. The innovation applying of the different types of axial-flow pump systems in a quantity pumping station projects is introduced respectively. From the perspective of technological development, the development trends and application prospects of axial-flow pump system are analyzed. The limitations of the traditional pump selection method are discussed, and the applicable rationality of a new method of pump selection for the axial-flow pump system is introduced. The variable angle adjustment formula and applicability based on the test data of axial-flow pump system are introduced. The harmfulness to the pump units from the intake vortex and the safety policies are analyzed. The research results of the vortices in the pump sump and the measures of vortices prevention and elimination are described. As regarding the real and potential problems in research development on axial-flow pump system, the suggestions for further deepen researches are presented. Although the hydraulic performance of axial flow pump system research has reached a higher level, but possibility of partial breakthroughs still exists. The future research direction of pump system should be more to the structural optimization, the pump system reliability and adaptability, vibration and noise, and run quality promotion. ©, 2015, Chinese Society of Agricultural Machinery. All right reserved. Source


Ueda Y.,University of Bonn | Frimpong F.,University of Bonn | Qi Y.,Yangzhou University | Matthus E.,University of Bonn | And 4 more authors.
Journal of Experimental Botany | Year: 2015

Tropospheric ozone causes various negative effects on plants and affects the yield and quality of agricultural crops. Here, we report a genome-wide association study (GWAS) in rice (Oryza sativa L.) to determine candidate loci associated with ozone tolerance. A diversity panel consisting of 328 accessions representing all subgroups of O. sativa was exposed to ozone stress at 60 nl l-1 for 7h every day throughout the growth season, or to control conditions. Averaged over all genotypes, ozone significantly affected biomass-related traits (plant height -1.0%, shoot dry weight -15.9%, tiller number -8.3%, grain weight -9.3%, total panicle weight -19.7%, single panicle weight -5.5%) and biochemical/physiological traits (symptom formation, SPAD value -4.4%, foliar lignin content +3.4%). A wide range of genotypic variance in response to ozone stress were observed in all phenotypes. Association mapping based on more than 30 000 single-nucleotide polymorphism (SNP) markers yielded 16 significant markers throughout the genome by applying a significance threshold of P<0.0001. Furthermore, by determining linkage disequilibrium blocks associated with significant SNPs, we gained a total of 195 candidate genes for these traits. The following sequence analysis revealed a number of novel polymorphisms in two candidate genes for the formation of visible leaf symptoms, a RING and an EREBP gene, both of which are involved in cell death and stress defence reactions. This study demonstrated substantial natural variation of responses to ozone in rice and the possibility of using GWAS in elucidating the genetic factors underlying ozone tolerance. © The Author 2014. Source


Li S.C.,Yangzhou University
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] | Year: 2012

Samples of chicken, duck, quail, and pigeon were collected from Jiangsu, Anhui, and Hebei in 2009-2011, and sixteen H9N2 subtype isolates of avian influenza virus (AIV) were identified. The eight full-length genes of 16 AIV isolates were amplified by RT-PCR and sequenced. Genome sequence analysis showed that the amino acid motif of cleavage sites in the HA gene was P-S-R/K-S-S-R, which was consistent with the characterization of the LPAIV, and the Leucine (L) at the amino acid position 226 in the HA genes of all isolates indicated the potential of binding with SAalpha, 2-6 receptor. All isolates had a S to N substitution at residue 31 in the M2 gene, which is related to the resistance phenotype of adamantanes. The key molecular features of 16 AIV isolates from different hosts were same. Genome phylogenetic analysis revealed that all 16 H9N2 subtype AIVs originated from F98-like virus as backbone and formed two new genotypes through reassortment with HA gene of Y280-like virus and PB2 and M genes of G1-like virus. Our findings suggest that more attention should be paid to the surveillance of H9N2 influenza virus and its direction of reassortment. Source


Tao Y.C.,Nanjing Normal University | Hu J.G.,Yangzhou University
Journal of Applied Physics | Year: 2010

Superconducting spintronics is one of the most attractive subjects of spintronics. This article reviews superconducting spintronics based on the superconducting junctions with ferromagnetic semiconducting contact. The authors summarize recent theoretical developments with an emphasis on the interplay between ferromagnetic semiconductor (FS) and superconductor (SC). It is found that the spin-polarized transport in the superconducting junctions exhibits a rich dependence on hole types of FS, mismatches in the effective mass and Fermi velocity of holes between the FS and SC, as well as strengths of potential scattering at the interface. These systems have great intrinsic scientific importance and potential device applications including signal processing and general purpose computing. © 2010 American Institute of Physics. Source


Fang Y.,Huazhong Agricultural University | Fang Y.,Yangzhou University | Xiong L.,Huazhong Agricultural University
Cellular and Molecular Life Sciences | Year: 2015

Plants often encounter unfavorable environmental conditions because of their sessile lifestyle. These adverse factors greatly affect the geographic distribution of plants, as well as their growth and productivity. Drought stress is one of the premier limitations to global agricultural production due to the complexity of the water-limiting environment and changing climate. Plants have evolved a series of mechanisms at the morphological, physiological, biochemical, cellular, and molecular levels to overcome water deficit or drought stress conditions. The drought resistance of plants can be divided into four basic types-drought avoidance, drought tolerance, drought escape, and drought recovery. Various drought-related traits, including root traits, leaf traits, osmotic adjustment capabilities, water potential, ABA content, and stability of the cell membrane, have been used as indicators to evaluate the drought resistance of plants. In the last decade, scientists have investigated the genetic and molecular mechanisms of drought resistance to enhance the drought resistance of various crops, and significant progress has been made with regard to drought avoidance and drought tolerance. With increasing knowledge to comprehensively decipher the complicated mechanisms of drought resistance in model plants, it still remains an enormous challenge to develop water-saving and drought-resistant crops to cope with the water shortage and increasing demand for food production in the future. © 2014 Springer Basel. Source


Ye X.-S.,Yangzhou University
Physica C: Superconductivity and its Applications | Year: 2011

We analyze the magnetic properties through two-orbital Hubbard model with the spin-orbit coupling (SOC) interaction in the iron-based superconductors. With the help of the Ising approximation for the Hund's coupling between the itinerant electrons and the localized spins, we give a self-consistent account of the various magnetic orders observed in pnictides and the pairing symmetry. We also calculate the local density of states (LDOS) of the vortex state when a magnetic field is applied. The LDOS without SOC shows no resonant peak at the vortex core center in the superconducting state, while it shows an obvious resonant peak when SOC is applied. © 2011 Elsevier B.V. All rights reserved. Source


Huo Z.,China Agricultural University | Dai X.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | Feng S.,Yangzhou University | Kang S.,China Agricultural University | Huang G.,China Agricultural University
Journal of Hydrology | Year: 2013

Temporal variations in reference evapotranspiration (ET0) and aridity index (AI) were comprehensively investigated for 23 meteorological stations during 1955-2008 in the northwest China. The quantitative contributions of the major meteorological variables to the ET0 and AI trends were evaluated and the possible causes were also investigated. The results showed that in the past 50years annual temperature, humidity and precipitation had significant increasing trends with time, and wind speed and radiation had decreasing trends. ET0 had a significant decreasing trend with an averagely value of about 3mm per year, and AI had also witnessed a decreasing trend; For ET0, wind speed was the most sensitive meteorological variable, followed by relative humidity, temperature and radiation and for AI, precipitation was the most sensitive meteorological variable. The contribution of wind speed to the decrease of ET0 is more than that of other meteorological variables. The increase of precipitation contributes more than the decrease of ET0 to decrease of AI in past 50years. This study provides an understanding of the effect of recent climate change on drought in arid northwest China. © 2013 Elsevier B.V. Source


Li J.,CAS National Center for Nanoscience and Technology | Han Q.,CAS National Center for Nanoscience and Technology | Wang X.,CAS National Center for Nanoscience and Technology | Wang X.,Yangzhou University | And 4 more authors.
Small | Year: 2014

A novel and convenient method to synthesize the nanocomposites combining graphene oxides (GO) with gold nanoparticles (AuNPs) is reported and their applications to modulate amyloid peptide aggregation are demonstrated. The nanocomposites produced by pulsed laser ablation (PLA) in water show good biocompatibility and solubility. The reduced aggregation of amyloid peptides by the nanocomposites is confirmed by Thioflavin T fluorescence and atomic force microscopy. The cell viability experiments reveals that the presence of the nanocomposites can significantly reduce the cytotoxicity of the amyloid peptides. Furthermore, the depolymerization of peptide fibrils and inhibition of their cellular cytotoxicity by GO/AuNPs is also observed. These observations suggest that the nanocomposites combining GO and AuNPs have a great potential for designing new therapeutic agents and are promising for future treatment of amyloid-related diseases. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Lingling W.,Yangzhou University
Journal of Leukemia and Lymphoma | Year: 2015

Objective To evaluate the relationship between proportion of PR1 specific cytotoxic T lymphocytes (CTLs) in the peripheral blood and prognosis and curative effect in patients with HLA-A0201 positive chronic myelogenous leukemia (CML), and to discuss whether PR1 peptide could be used as the following immune therapeutic method for patients who had achieved the standard of stop treatment. Methods The soluble HLA-A0201/PR1 tetramer and flow cytometry were applied to determine the proportion and the frequency of PR1 specific CTLs in peripheral blood from 28 HLA-A0201 positive CML patients. The proportions were compared among different phases of patients. The correlations between the proportion of PR1 specific CTLs and clinical parameters were analyzed. Results There was a negative correlation between PR1 specific CTLs and PCR (bcr-abl/abl)IS (r=-0.658, P < 0.001). The frequencies of PR1 specific CTLs at 3-month, 6-month, 9-month, 12-month, 2-year, 3-year, 4-year, 5-year, 6-year were (0.06 ±0.02) %, (0.10 土 0.02) %, (0.14±0.02) %, (0.16±0.02) %, (0.20±0.03) %, (0.18±0.03) %, (0.18±0.01) %, (0.17±0.05) % and (0.18±0.03) %, respectively. The frequency of PR1 specific CTLs at 3-month, 6-month or 9-month was statistically different compared with that of the other time spots (P < 0.05), and there were no statistical differencies among the frequencies at 1 -year, 2-year, 3-year, 4-year, 5-year, 6-year (P > 0.05). For patients treated with IM 400 mg qd, the frequency of PR1 specific CTLs in high-risk group was lower than that in low-risk or intermediate-risk groups. Conclusion PR1 specific CTLs can be detected in patients who achieved good curative effect, and is correlated with tumor burden, which indicates that PR1 specific CTLs may be related to the action of resisting leukemia and provide the evidence for PR1 peptide as a potential immune therapeutic schedule in patients who have achieved stable MR4.5 and MR5.0. © 2015 Editorial Board of Journal of Leukemia and Lymphoma. All rights reserved. Source


Junliang L.,Yangzhou University | Junliang L.,Nanjing University of Technology | Yanwei Z.,Nanjing University of Technology | Cuijing G.,Nanjing University of Technology | And 2 more authors.
Journal of the European Ceramic Society | Year: 2010

Single phase barium hexaferrite nano-powders have been innovatively one-step synthesized via a microwave-assisted sol-gel auto-combustion in a specially designed quartz vessel using citric acid and ethylene diamine tetraacetic acid as composite chelating agents and freeze-drying technique to remove sols' moisture. The auto-combustion product powder is characterized by fluffy particle aggregates with the crystallites ranging from 50 to 100 nm in diameter and containing single magnetic domains for each with a low apparent coercive field of 260 Oe and a high saturation magnetization of 64.1 emu/g. The direct formation of barium hexaferrite is believed to result from the effective improvement in the spatial distribution homogeneity of metal ions and oxidant in the gels. Moreover, the quartz vessel with barium hexaferrite ceramic pad on sample's support and film-strips on its interior wall can effectively build up a favorable temperature environment to promote the direct formation of barium hexaferrite with microwave assistance during the gel's uniform auto-combustion. © 2009 Elsevier Ltd. All rights reserved. Source


Li X.Q.,Yangzhou University | Zhu P.,No. 401 Hospital | Myatt L.,University of Texas Health Science Center at San Antonio | Sun K.,Shanghai JiaoTong University | Sun K.,University of Texas Health Science Center at San Antonio
Placenta | Year: 2014

The pivotal role of glucocorticoids in the initiation of parturition has been very well documented in several domestic mammalian animal species. However the role of glucocorticoids in human parturition remains controversial mainly because of the absence of effect of synthetic glucocorticoids, given to promote fetal organ maturation in pregnant women with threatened preterm delivery, on the length of gestation. This article will review studies of glucocorticoids in human parturition and provide evidence for an important role of glucocorticoids in human parturition as well but a simultaneous high concentration of estrogen within the intrauterine tissues may be necessary for GCs to initiate parturition. The synthetic GCs dexamethasone and betamethasone pass through the placenta intact resulting in potent negative feedback on the fetal HPA axis and diminished production of DHEA from fetal adrenal glands for estrogen synthesis by the placenta. This may negate the effect of systemic administration of GCs on the induction of labor, especially in cases where the myometrium is not yet fully primed by estrogen. Endogenous glucocorticoids are inactivated by the placental 11β-HSD2 thus limiting the negative feedback of maternal cortisol on the fetal HPA axis and allowing the simultaneous rise of cortisol and estrogen levels towards the end of gestation. Therefore, endogenous glucocorticoids, particularly glucocorticoids produced locally in the intrauterine tissues may play an important role in parturition in humans by enhancing prostaglandin production in the fetal membranes and stimulating estrogen and CRH production in the placenta. © 2014 Elsevier Ltd. All rights reserved. Source


Qian L.,Second Military Medical University | Qian L.,Yangzhou University | Qian C.,Second Military Medical University | Chen Y.,Second Military Medical University | And 5 more authors.
Blood | Year: 2012

Regulatory dendritic cells (DCs) play important roles in the induction of peripheral tolerance and control of adaptive immune response. Our previous studies demonstrate that splenic stroma can drive mature DCs to proliferate and further differentiate into a unique subset of CD11b hiIa low regulatory DCs, which could inhibit T-cell response, program generation of immunosuppressive memory CD4 T cells. However, the effect of regulatory DCs on B-cell function remains unclear. Here, we report that regulatory DCs can induce splenic B cells to differentiate into a distinct subtype of IL-10-producing regulatory B cells with unique phenotype CD19 hiFcγIIb hi. CD19 hiFcγIIb hi B cells inhibit CD4 T-cell response via IL-10. CD19 hiFcγIIb hi B cells have enhanced phagocytic capacity compared with conventional CD19 + B cells, and FcγRIIb mediates the uptake of immune complex by CD19 hiFcγIIb hi B cells. We found that regulatory DC-derived IFN-β and CD40 ligand are responsible for the differentiation of CD19 hiFcγIIb hi B cells. Furthermore, an in vivo counterpart of CD19 hiFcγIIb hi B cells in the spleen and lymph nodes with similar phenotype and regulatory function has been identified. Our results demonstrate a new manner for regulatory DCs to down-regulate immune response by, at least partially, programming B cells into regulatory B cells. © 2012 by The American Society of Hematology. Source


Li W.,Yangzhou University | Li W.,Jiangsu Key Laboratory Of Intgd Traditional Chinese And W Med For Prev And Treatm Of Senile Diseases | Xing Y.,Dalian University of Technology | Liu Y.,Dalian University of Technology
Cancer Chemotherapy and Pharmacology | Year: 2015

Drug combinations including irinotecan and gefitinib have been evaluated in clinical trials. SN-38 is the active metabolite of irinotecan, and the increase in its concentration due to drug interactions will result in increased clinical toxicity. We aimed to investigate the effects of gefitinib and its predominant metabolite observed in human plasma, O-desmethyl-gefitinib (DMG), on SN-38 glucuronidation. Our data indicated that both gefitinib and DMG are potent inhibitors of SN-38 glucuronidation via UGT1A1 inhibition. It is predicted from in vitro data that gefitinib administered at 700 mg/day may result in about 149 % increase in SN-38 AUC, but there is no significant effects on SN-38 AUC at lower concentrations. Our prediction study provides a basis for design of clinical studies for the development and optimization of this combination. © 2015 Springer-Verlag Berlin Heidelberg. Source


Zhang Q.,Yangzhou University | Tian C.,Yancheng Institute of Technology
Nonlinear Dynamics | Year: 2014

This paper is concerned with the pattern formation and pattern dynamics of a diffusive Rössler model. We first show that the time-delay and the cross-diffusion can lead to Hopf bifurcation and Turing bifurcation, respectively, by computing Lyapunov characteristic exponent. Then by the calculation of the first Lyapunov number and weak nonlinear analysis, the dynamics of Hopf pattern and Turing pattern is investigated. Our results reveal that Hopf bifurcation generates the transient spiral wave, but the spiral wave will break up and becomes the terminate irregular pattern. Turing bifurcation generates a stable spotted pattern. © 2014, Springer Science+Business Media Dordrecht. Source


Jin W.,Yangzhou University
Quantum Information Processing | Year: 2016

We discuss at length the dynamical behavior of Grover’s search algorithm for which all the Walsh–Hadamard transformations contained in this algorithm are exposed to their respective random perturbations inducing the augmentation of the dimension of the search space. We give the concise and general mathematical formulations for approximately characterizing the maximum success probabilities of finding a unique desired state in a large unsorted database and their corresponding numbers of Grover iterations, which are applicable to the search spaces of arbitrary dimension and are used to answer a salient open problem posed by Grover (Phys Rev Lett 80:4329–4332, 1998). © 2015, Springer Science+Business Media New York. Source


Xu X.,Nanjing Southeast University | Xu X.,Yangzhou University | Xu C.,Nanjing Southeast University | Lin Y.,Nanjing Southeast University | And 2 more authors.
Journal of Physical Chemistry C | Year: 2013

We carried out a detailed comparison on the defect-related photoluminescence and magnetism in two kinds of undoped ZnO nanorods synthesized by low-temperature chemical bath deposition and high-temperature chemical vapor deposition methods to clarify further the nature of visible emission and d 0 ferromagnetism in ZnO based on the fact that these two kinds of ZnO nanorods have significantly different crystallinity and defect states. The results obtained by analyzing X-ray diffraction, electron paramagnetic resonance, and Raman scattering show that the intrinsic ferromagnetism in ZnO nanorods is originated from the single ionized oxygen defects (vacancies and interstitials). Particularly, it is demonstrated that the lattice disorder along the c-axis along with the introduced oxygen interstitials can boost ferromagnetism and induce the red-shift in visible emission. © 2013 American Chemical Society. Source


Yang J.,Yangzhou University | Zhang J.,Hong Kong Baptist University
Journal of Experimental Botany | Year: 2010

Modern rice (Oryza sativa L.) cultivars, especially the newly bred 'super' rice, have numerous spikelets on a panicle with a large yield capacity. However, these cultivars often fail to achieve their high yield potential due to poor grain-filling of later-flowering inferior spikelets (in contrast to the earlier-flowering superior spikelets). Conventional thinking to explain the poor grain-filling is the consequence of carbon limitation. Recent studies, however, have shown that carbohydrate supply should not be the major problem because they have adequate sucrose at their initial grain-filling stage. The low activities of key enzymes in carbon metabolism may contribute to the poor grain-filling. Proper field practices, such as moderate soil drying during mid-and late grain-filling stages, could solve some problems in poor grain-filling. Further studies are needed by molecular approaches to investigate the signal transport, the hormonal action, the gene expressions, and the biochemical processes in inferior spikelets. Source


Rao S.-Q.,Jiangnan University | Rao S.-Q.,Yangzhou University | Ju T.,Jiangnan University | Sun J.,Jiangnan University | And 3 more authors.
Food Research International | Year: 2012

The aims of this study were to explore new functional bioactivity of hen egg white lysozyme (HEWL) protein hydrolysate and to identify potential angiotensin I-converting enzyme (ACE) inhibitory peptides from HEWL. The protein was hydrolyzed by gastrointestinal enzymes, including pepsin, α-chymotrypsin and trypsin, under simulated physiological conditions, and the hydrolysate exhibited potent ACE inhibitory activity with an IC 50 value of 15.6±1.4μg/mL. Three novel ACE inhibitory peptides, specifically, Met-Lys-Arg, Arg-Gly-Tyr and Val-Ala-Trp with IC 50 values of 25.7±0.2, 61.9±0.1 and 2.86±0.08μM, respectively, were isolated from the hydrolysate of HEWL in two stages of reverse-phase high-performance liquid chromatography (RP-HPLC), and their sequences were analyzed by UPLC-MS/MS. Kinetics studies suggested that the purified peptides were all competitive inhibitors; preincubation experiments implied that the peptide Arg-Gly-Tyr was a true substrate and that the other two peptides were true ACE inhibitors. These results indicate that HEWL may become an effective source of ACE inhibitory peptides. This study reveals the antihypertensive bioactivity of HEWL protein hydrolysate and provides further evidence that eggs are an excellent source of health-promoting food. © 2011 Elsevier Ltd. Source


Su D.,The Clean Tech Center | Wang C.,Yangzhou University | Ahn H.,Gyeongsang National University | Wang G.,The Clean Tech Center
Physical Chemistry Chemical Physics | Year: 2013

Single crystalline SnO2 nanocrystals (∼60 nm in size) with a uniform octahedral shape were synthesised using a hydrothermal method. Their phase and morphology were characterized by XRD and FESEM observation. TEM and HRTEM analyses identified that SnO2 octahedral nanocrystals grow along the [001] direction, consisting of dominantly exposed {221} high energy facets. When applied as anode materials for Na-ion batteries, SnO2 nanocrystals exhibited high reversible sodium storage capacity and excellent cyclability (432 mA h g-1 after 100 cycles). In particular, SnO 2 nanocrystals also demonstrated a good high rate performance. Ex situ TEM analysis revealed the reaction mechanism of SnO2 nanocrystals for reversible Na ion storage. It was found that Na ions first insert into SnO2 crystals at the high voltage plateau (from 3 V to ∼0.8 V), and that the exposed (1 × 1) tunnel-structure could facilitate the initial insertion of Na ions. Subsequently, Na ions react with SnO2 to form NaxSn alloys and Na2O in the low voltage range (from ∼0.8 V to 0.01 V). The superior cyclability of SnO 2 nanocrystals could be mainly ascribed to the reversible Na-Sn alloying and de-alloying reactions. Furthermore, the reduced Na2O "matrix" may help retard the aggregation of tin nanocrystals, leading to an enhanced electrochemical performance. This journal is © the Owner Societies 2013. Source


Ji L.,Imperial College London | Ji L.,Yangzhou University | Jell G.,Imperial College London | Jell G.,University College London | And 3 more authors.
Chemical Communications | Year: 2011

Hydroxyapatite has found wide application in bone tissue engineering. Here we use a macroporous carbon template to generate highly ordered macroporous hydroxyapatite bioceramics composed of close-packed hollow spherical pores with interconnected channels. The template has advantages for the preparation of ordered materials. © 2011 The Royal Society of Chemistry. Source


Jin Z.,Shanghai JiaoTong University | Jin Z.,Yangzhou University | Cui Z.,Shanghai JiaoTong University
Journal of Materials Science and Technology | Year: 2010

A modified cellular automata (CA) model of dynamic recrystallization (DRX) and a flow stress-based nucleation parameter identification method have been developed. In the method, the modified CA model, which takes the role of deformation degree on nucleation behavior into consideration, is coupled with an adaptive response surface model (ARSM) to search for the optimum nucleation parameter. The DRX behavior of an oxygen free high conductivity (OFHC) copper with different initial grain sizes has been taken as an example to validate the model. Good agreement is found between the simulated and the experimental results, which demonstrates that the new method can effectively improve the simulation accuracy. © 2010 The Chinese Society for Metals. Source


Objective: To study the effect of exercise intervention on left ventricular hypertrophy in spontaneously hypertensive rats(SHR) by determining the mRNA expression of natriuretic peptide receptor A(NPRA) and the contents of atrial natriuretic peptide (ANP), cyclic guanosine monophosphate (cGMP) in cardiac muscle. Method: Sixteen male SHR were randomly divided into SHR control group(SC, n=8) and SHR exercise training group (SE, n=8), another eight male Wistar rats were as normal matched control group (WC). SE group carried on 60min swimming 6 times a week, all rats feed normal feedstuff for 8 weeks. And then the rats' blood pressure were determined weekly, the contents of ANP were detected by radioimmunoassay, cGMP in cardiac muscle by ELISA, and the mRNA expression of NPR in cardiac muscle by real time-PCR. Result: After 8-week exercise, BP of SE group was little higher than before, but no significant difference. However, that of SC group was just opposite (P<0.01); To compared with SC group, in SE group BP, index of HWI and LVI decreased dramatically(P<0.01), the level of ANP increased, but no significant difference; the level of NPR mRNA expression and cGMP content elevated markedly(P<0.01). Conclusion: The 8-week 60-min swimming exercise can increase the ANP amount of plasma in SHR. Meanwhile, the expression of NPR-A mRNA (the ANP receptor) and content of cGMP in cardiac muscle also increased, then ANP play a role against myocardial hypertrophy and proliferation. Source


Wang J.,Jiangsu University | Wang J.,Anhui University of Science and Technology | Wang J.,CAS Hefei Key Laboratory of Materials for Energy Conversion | Chen K.,Jiangsu University | And 3 more authors.
Nano Letters | Year: 2013

The catalytic mechanism offers an efficient tool to produce crystalline semiconductor nanowires, in which the choice, state, and structure of catalysts are active research issues of much interest. Here we report a novel solution-solid-solid (SSS) mechanism for nanowire growth catalyzed by solid-phase superionic conductor nanocrystals in low-temperature solution. The preparation of Ag2Se-catalyzed ZnSe nanowires at 100-210 C is exampled to elucidate the SSS model, which can be extendable to grow other II-VI semiconductor (e.g., CdSe, ZnS, and CdS) nanowires by the catalysis of nanoscale superionic-phase silver or copper(I) chalcogenides (Ag2Se, Ag2S, and Cu2S). The exceptional catalytic ability of these superionic conductors originates from their structure characteristics, known for high-density vacancies and fast mobility of silver or copper(I) cations in the rigid sublattice of Se2- or S2- ions. Insights into the SSS mechanism are provided based on the formation of solid solution and the solid-state ion diffusion/transport at solid-solid interface between catalyst and nanowire. © 2013 American Chemical Society. Source


Hao D.,Nanjing Agricultural University | Hao D.,Jiangsu Yanjiang Institute of Agricultural science | Cheng H.,Nanjing Agricultural University | Yin Z.,Yangzhou University | And 4 more authors.
Theoretical and Applied Genetics | Year: 2012

Genome-wide association analysis is a powerful approach to identify the causal genetic polymorphisms underlying complex traits. In this study, we evaluated a population of 191 soybean landraces in five environments to detect molecular markers associated with soybean yield and its components using 1,536 single-nucleotide polymorphisms (SNPs) and 209 haplotypes. The analysis revealed that abundant phenotypic and genetic diversity existed in the studied population. This soybean population could be divided into two subpopulations and no or weak relatedness was detected between pair-wise landraces. The level of intra-chromosomal linkage disequilibrium was about 500 kb. Genome-wide association analysis based on the unified mixed model identified 19 SNPs and 5 haplotypes associated with soybean yield and yield components in three or more environments. Nine markers were found co-associated with two or more traits. Many markers were located in or close to previously reported quantitative trait loci mapped by linkage analysis. The SNPs and haplotypes identified in this study will help to further understand the genetic basis of soybean yield and its components, and may facilitate future high-yield breeding by marker-assisted selection in soybean. © 2011 Springer-Verlag. Source


Wang Y.,Nanjing Southeast University | Liu Q.,Nanjing Southeast University | Qi Q.,Nanjing Southeast University | Ding J.,Hefei University of Technology | And 3 more authors.
Electrochimica Acta | Year: 2013

The magnetite/reduced graphene oxide (Fe3O4/RGO) composite was synthesized by a facile one-pot in-situ method and characterized by transmission electron microscopy, atomic force microscopy, vibratingsample magnetometer, X-ray powder diffraction, surface area and pore size distribution, thermogravi-metric analysis, Raman spectroscopy and X-ray photoelectron spectroscopy, respectively. The compositeconsisted of the RGO substrate with a single-layered Fe3O4nanoparticle film, which showed the largesurface area of 352 m2g-1and the saturated magnetization of 26.7 emu g-1. The average diameter andcoverage ratio of Fe3O4were about 8 nm and 50%, respectively. The electrocatalytic oxidation anddetection of N-acetylcysteine (NAC) based on composite-modified glassy carbon electrodes (GCE) wereinvestigated by cyclic voltammetry, amperometry, double potential step chronoamperometry and differ-ential pulse voltammetry. Especially, the kinetic performance of oxidation processes were estimated bythe Cottrell equation, indicating the diffusion coefficient of 2.06 × 10-5cm2s -1and the catalytic reactionrate constant of 3.47 × 10 6cm3mol-1s-1. Compared with the kinetic parameters, it was found that theoxidation process included both slow absorption and quick diffusion processes. The detection limit ofNAC was 1.11 × 10-5mol L-1 in the range of 0.10-10.0 mmol L -1.© 2013 Elsevier Ltd. Source


Yang D.-G.,Suzhou University | Liu J.-L.,Yangzhou University
Mathematical and Computer Modelling | Year: 2010

Let q be analytic in the open unit disk U with q(0)=1 and q(z)≠0(z∈U). By using the method of differential subordinations, we derive certain conditions involving q and zq' under which the functions q satisfy the following two-sided inequality: for some α 1 and α 2(0<α 1,α2≤1). Several interesting consequences of the main results are also given. All these results presented here are sharp. © 2010 Elsevier Ltd. Source


Wu S.,Yangzhou University | Tomic S.,University of Salford
Journal of Applied Physics | Year: 2012

The effects of a transverse electric field on the electronic structure, exciton states, and oscillator strengths in a cylindrical quantum wire (QWR) are theoretically investigated. We consider a QWR made of GaAs material surrounded by a barrier of Al 0.3Ga 0.7As of finite depth potential. The electronic structure of the QWR, at the single electron level of theory, is obtained inside the effective mass approximation using the plane wave method, while the exciton states and transition oscillator strengths are calculated using the variational principle. The results show that the exciton oscillator strength is strongly enhanced due to the excitonic effect. The external electric field lifts the degeneracy of the electron or hole states. The energy levels of the electron and hole, exciton binding energy and exciton oscillator strength decrease with the increasing of the strength of the electric field. The stronger the electric field, the weaker the excitonic effect is. The influence of the electric field on exciton states and oscillator strengths becomes more significant for wide quantum wires. However, in sufficiently narrow wires, the influence of the electric field is also significant at small fields. © 2012 American Institute of Physics. Source


Yixiang B.,Yangzhou University | Jinhao Q.,Nanjing University of Aeronautics and Astronautics
International Journal of Applied Electromagnetics and Mechanics | Year: 2011

The metal core piezoelectric fiber (MPF) is one of the new type piezoelectric devices for sensors and actuators. To investigate the dynamic characteristics of a cantilever MPF, the dynamic admittance matrix is established. It can describe the response of a cantilever MPF subjected to driving parameters, a harmonically varying axial force F at the tip and a harmonically varying voltage V on the electrodes. The responses parameters of a cantilever MPF, including a dynamic tip elongation δ and a dynamic electric charge Q, can be obtained using the vibration theory and the average distributed electrical charge method. The response parameters are related to the driving parameters by a 2 × 2 matrix. The dynamic models for measuring piezoelectric parameters of a MPF are given in order to illustrate the applications of the previous dynamic admittance matrix. The piezoelectric parameters, the elastic coefficient s11E , the piezoelectric coefficient d31 and the dielectric constant ε33T of MPF were measured experimentally. © 2011 - IOS Press and the authors. All rights reserved. Source


Chen L.,China University of Petroleum - East China | Zhang X.,Yangzhou University
Tenside, Surfactants, Detergents | Year: 2013

This paper evaluated the oil displacing performance of novel surfactants called dodecyl polyoxyethylene ethers sulfonates (AESO) at high-temperature, high-salinity and low-permeability reservoir conditions. We found that AESO-9 and AESO-12 with better thermal stability could create ultra-low interfacial tension (IFT) at high-temperature and high-salinity conditions, and the IFT was largely independent of both temperature and salinity. Compared to AES-12, temperature and salinity had much less effect on the emulsification property of AESO-12. The dynamic equilibrium adsorption capacity of AESO-12 was only 0.09 mg/g sand at 90 °C. The incremental recovery by only using AESO-12 could attain 8.81 % at difficult conditions. When the core permeability decreased to 2.67 × 10-3 μm2, the incremental recovery by using the composite oil displacement agents based on AESO-12 could still attain 10.98% at the temperature of 90°C and the salinity of 60g/L. In brief, AESO-12 exhibits excellent oil-displacement capacity at high-temperature, high-salinity and low-permeability reservoir conditions. Source


Wu J.,Yangzhou University
World journal of surgical oncology | Year: 2014

CT findings in three cases with solitary fibrous tumors (SFTs) confirmed by histopathology and immunohistochemistry were reviewed retrospectively, and compared with pathological results. The three tumors were large, well-defined, and smooth contour masses and SFT consisted of solid components of two different densities. On enhanced CT scans, tumors were strongly enhancing, the multiple vascular shadows were seen within the tumor in the arterial phase. There is progressive enhancement from the arterial to the venous phase, and the tumor capsule can be observed. Histologically, the tumors are composed of spindle cells within a background of collagen stroma, and showed a wide range of growth patterns, alternating hypercellular (tumor cell-rich) and hypocellular (collagen-rich) areas. The diagnosis is confirmed by characteristic positive immunohistochemical staining for CD34. Source


Chu H.J.,Los Alamos National Laboratory | Chu H.J.,Yangzhou University | Wang J.,Los Alamos National Laboratory | Zhou C.Z.,Los Alamos National Laboratory | Beyerlein I.J.,Los Alamos National Laboratory
Acta Materialia | Year: 2011

In this work we investigate the self-energy of elliptical dislocation loops in anisotropic crystals and determine the functional dependencies on loop circumference, shape, and dislocation core radius. Systematic numerical calculations using the anisotropic point force Green's function method are carried out with the goal of developing an analytical expression for the self-energy associated with these loops. The resulting formula is shown to accurately predict the self-energies for elliptical loops in anisotropic crystals, as well as the self-energies for simple loop configurations in isotropic crystals, for which analytical expressions exist. We apply this expression to predict the critical shell thickness corresponding to defect-free core/shell nanowires (NW) and further for the first time consider the effect of image energy due to the finite size of NW in anisotropic media using the boundary element method. Consequently, self-energy in NWs is corrected by an energy factor. Moreover, we discuss the dependence of the critical shell thickness on growth direction, with 〈1 1 0〉 NW having the largest, 〈1 1 1〉 NW the next largest, and 〈1 1 2〉 NW the finest. © 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. Source


Yan X.,Yangzhou University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2012

OBJECTIVE To develop a PCR method for detecting the newly reported genotype (variant 2c, V2c) of Mycoplasma pneumoniae strains. Specific primer was designed for detecting the V2c type based on the variant region of V2c strain p1 gene. A nested multiple PCR method for V2c strain detection was set up and confirmed by related gene sequencing. In total 214 clinical strains isolated from Beijing between 2008 and 2011 were analyzed by this typing method. Nest multiple PCR typing method is effective to detect the V2c strain. Of the 214 M. pneumoniae strains 90.2% (193/214) were type 1, 0.9% (2/214) were variant 2a, and 8.9% (19/214) were V2c. No type 2 was detected. This typing method is effective to distinguish the V2c strains from other variant M. pneumoniae strains, and important for the epidemiological study of Mycoplasma pneumonia infection. Source


Xu W.,Chinese University of Hong Kong | Xu W.,CAS Nanjing Institute of Soil Science | Jia L.,Chinese University of Hong Kong | Jia L.,Inner Mongolia Agricultural University | And 5 more authors.
New Phytologist | Year: 2013

Maintenance of root growth is essential for plant adaptation to soil drying. Here, we tested the hypothesis that auxin transport is involved in mediating ABA's modulation by activating proton secretion in the root tip to maintain root growth under moderate water stress. Rice and Arabidopsis plants were raised under a hydroponic system and subjected to moderate water stress (-0.47 MPa) with polyethylene glycol (PEG). ABA accumulation, auxin transport and plasma membrane H+-ATPase activity at the root tip were monitored in addition to the primary root elongation and root hair density. We found that moderate water stress increases ABA accumulation and auxin transport in the root apex. Additionally, ABA modulation is involved in the regulation of auxin transport in the root tip. The transported auxin activates the plasma membrane H+-ATPase to release more protons along the root tip in its adaption to moderate water stress. The proton secretion in the root tip is essential in maintaining or promoting primary root elongation and root hair development under moderate water stress. These results suggest that ABA accumulation modulates auxin transport in the root tip, which enhances proton secretion for maintaining root growth under moderate water stress. © 2012 New Phytologist Trust. Source


Zhang G.,University of Cincinnati | Zhang Y.C.,Yangzhou University | Nadagouda M.,National Risk Management Research Laboratory | Han C.,University of Cincinnati | And 4 more authors.
Applied Catalysis B: Environmental | Year: 2013

Motivated from the increasing environmental concerns associated with the formation of cyanotoxins released by cyanobacteria in waters, this study focused on the synthesis and evaluation of visible light-sensitized S, N and C co-doped polymorphic titanium dioxide (CDPM-TiO2) nanoparticles for photocatalytic destruction of microcystin-LR, one of the most common and toxic cyanotoxins. The CDPM-TiO2, containing anatase, brookite and rutile phase, was synthesized using a modified sol-gel method followed by calcination at 300600°C. Thiourea was utilized as a precursor for the dopants. This work took the initiative to have detailed characterization on the co-doped polymorphic TiO2 by several techniques and utilize the CDPM-TiO2 on cyanotoxin treatment. The results showed that the physicochemical properties of CDPM-TiO2 samples were highly dependent on the calcination temperature. The CDPM-TiO2 sample calcined at 300°C (CDPM300) exhibited better physicochemical characters including higher surface area and stronger photo-absorption in the visible light region. The sulfur dopant was attributed to S6+ species; nitrogen was ascribed to interstitial N; carbon was assigned to the TiOC bond. Moreover, CDPM300 showed highest photocatalytic activity for microcystin-LR destruction under visible light irradiation among all CDPM-TiO2 nanoparticles, which can be considered as a promising demonstration of such visible light-sensitized photocatalysts in the treatment of an important cyanotoxin in water. © 2013 Elsevier B.V. Source


Pan Z.Y.,Yangzhou University
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2011

Using the PCR-SSCP method, the genetic variation in exon 1 of the TLR4 gene was detected among 893 animals, including Asian wild boars, 3 imported commercial and 10 Chinese indigenous swine breeds. This was conducted to analyze the polymorphisms of exon 1 of TLR4 gene in native and foreign pig breeds and aimed at providing a theoretical foundation for further research on the role that TLR4 gene played in immune and defense system. New alleles were isolated for exon 1 of the swine TLR4 gene for the first time, There were 6 genotypes and 3 alleles, in which the Duroc appeared AA, BB, CC, AB, AC and BC genotypes; Sutai pig, which has Duroc pig origin, were detected to be BB, CC, and BC genotypes; Yorkshire and Landrace were detected to be CC and BC genotypes. Wild boar and all 10 Chinese native pig breeds appeared highly conserved in exon 1 of TLR4 gene, with only CC genotype. Among the 3 homozygous genotypes, the CC genotype matches the sequence in GenBank, while a G93C synonymous mutation and a G194A nonsense mutation were found in the BB and AA genotypes, respectively. The correlation between these two mutation points of TLR4 gene with resistance to stress and disease is worthy of further study. Source


Zhu C.,Yangzhou University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011

In order to reveal why SEF14 fimbriae are restrictively expressed on strains of serogroup D salmonella, mainly S. enteritidis and S. dublin, the difference and variation of the sef14 operon gene clusters in S. enteritidis and related serogroup-D Salmonella were analyzed. The genes encoding subunits of sefA, sefD and sefR in S. pullorum, S. enteritidis and S. dublin were amplified by PCR method and then sequenced to analyze the the difference and variation, respectively. The results of PCR amplification showed that prevalence of sefA, sefD and sefR genes in S. enteritidis and S. dublin was 100%. In 18 isolates of S. pullorum, the prevalence of sefA gene was 100%,while the prevalence of sefD and sefR genes was 38.9% (7/18), and 11 strains isolated after 1980s did not contain any gene sefDor sefR. The sequencing data of PCR products revealed that sequences of sefA, sefD and sefR genes in S. enteritidis and S. dublin were identical with those those from NCBI GenBank data which accession number were L11008, U07129 and AF233854, respectively. Interestingly, among the 7 strains of S. pullorum before 1980s, the sefD sequence has a missing base pair at position 196 and caused open reading frame (ORF) shift, resulting in a stop codon (TAG) at position 71 amino acid residual (Leu of TTA at position 214 - 216 shift into stop codon of TAG at position 215 - 217). Unlike S. pullorum, all S. enteritidis and S. dublin tested could express SEF14 fimbriae in vitro. Based on the data of the difference and variation of sef14 operon gene clusters between S. enteritidis and S. pullorum, we may explain why SEF14 fimbriae in S. pullorum could not be expressed. Source


Hu K.,Yangzhou University
Journal of Theoretical and Applied Information Technology | Year: 2012

Trajectory clustering can predict moving trend of objects effectively. The traditional trajectory clustering algorithms take moving trajectory of a whole object as a research object, which will lose similar subtrajectories. However, in practical applications, such as in RFID system, the users may only focus on some specific regions of trajectories. We propose PT-CLUS algorithms in this paper, according to coarse-fine algorithm, which first dividing a trajectory into a group of line segments and prunes by coarse-fine strategy, and then searching cluster in the sub-trajectories by checking neighborhood region of segments, using hierarchical clustering to accomplish the clustering of sub-trajectories. Experiment result shows that PTCLUS algorithm can find the similar sub-trajectories from RFID trajectory database effectively. © 2005 - 2012 JATIT & LLS. All rights reserved. Source


Ye J.,University of Maryland University College | Shao H.,University of Maryland University College | Shao H.,Yangzhou University | Perez D.R.,University of Maryland University College
Immunotherapy | Year: 2012

Although vaccination significantly reduces influenza severity, seasonal human influenza epidemics still cause more than 250,000 deaths annually. Vaccine efficacy is limited in high-risk populations such as infants, the elderly and immunosuppressed individuals. In the event of an influenza pandemic (such as the 2009 H1N1 pandemic), a significant delay in vaccine availability represents a significant public health concern, particularly in high-risk groups. The increasing emergence of strains resistant to the two major anti-influenza drugs, adamantanes and neuraminidase inhibitors, and the continuous circulation of avian influenza viruses with pandemic potential in poultry, strongly calls for alternative prophylactic and treatment options. In this review, we focus on passive virus neutralization strategies for the prevention and control of influenza type A viruses. © 2012 Future Medicine Ltd. Source


Ye L.,Yangzhou University
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2011

Based on the established resource populations of Sutai pig, the expression of BPI gene was assayed by Real-time PCR to detect the tissue expression and analyze the differential expression between Escherichia coli F18-resistant and sensitive piglets. This study aimed at providing a theoretical foundation for further research on the role BPI gene in host immunity and resistance to E. coli F18. The results showed that the expression of BPI gene was extremely low or undetectable in tissues including heart, liver, spleen, lung, kidney, stomach, muscle, thymus, and lymph nodes, which was in a stark contrast to the significantly high levels in duodenum and jejunum. In the tissues of both jejunum and duodenum, the mRNA expression of BPI gene in resistant individuals was significant higher than that in the sensitive individuals (Pamp;0.05). The results suggested that BPI gene was likely to be related to the intestinal infection caused by E. coli F18. It is possible that the increased expression of BPI gene in intestinal is in connection with the resistance to E. coli F18. Source


Purely nanostructured polyaniline with the conductivity of 7.2 S cm -1 was synthesized via the quick addition of the oxidant of the solid ammonium peroxydisulfate into a cooled solution containing aniline and hydrochloric acid without any templates. The morphology of polyaniline is constructed of interwoven fibers with an average diameter of about 50 nm with lengths varying from 250 nm to 370 nm. In general, the conventional polyaniline completely lost its electric activity including conductivity and redox activity at pH 6; however, the polyaniline reported here shows two pairs of redox peaks on its cyclic voltammogram in 1.0 M NaCl solution with pH 7.0, which is similar to that of the conventional polyaniline in the more acidic solutions; and it still holds the redox activity until pH 9.0. The pH dependence of conductivity of polyaniline is also improved compared to that of the conventional polyaniline. The ESR measurements show that the deprotonated polyaniline still holds rather high unpaired spin densities. The 1H NMR spectra of polyaniline synthesized using interface polymerization are different those of the conventional polyaniline. The electrochemical behavior and spectra of polyaniline synthesized via the quick addition of an oxidant solution into a solution of aniline were reported and discussed. © 2010 Elsevier B.V. Source


Cui S.,Yangzhou University | Cui S.,Helmholtz Center for Infection Research | Wienhoefer N.,Helmholtz Center for Infection Research | Bilitewski U.,Helmholtz Center for Infection Research
International Immunopharmacology | Year: 2014

Genistein is a well known natural compound which is present in soy foods and exerts many beneficial functions such as anticancer, anti-inflammatory and antioxidant. However, until now little is known about the effects of genistein on the function of macrophages. The murine macrophage cell line RAW264.7 was used as target cell line. The results show that at concentrations of 50-100 μM, genistein reduced cell viability to 70%-80% (after 24 h) and 50%-60% (after 48 h), which was due to G2/M phase cell cycle arrest. Treatment of the macrophages with genistein for 24 or 48 h also led to significant morphological changes, such as elongation of the cells and development of long pseudopodia-like protrusions. By staining the F-actin cytoskeleton, we observed accumulation of actin-filaments at the edges of the cells. The morphology change and G2/M phase arrest after genistein treatment is due to the activation of the phosphorylation of MAP kinase p38. The morphology change and cell cycle arrest can be significantly reverted when treatment is combined with p38 inhibitor SB203580. Moreover, after treatment of the macrophages with genistein for 24 and 48 h, the phagocytotic efficiency for Candida albicans was decreased in a time- and dose-dependent manner which correlates to the morphology change. The production of cytokines (TNF-α) stimulated by C. albicans was strongly inhibited by genistein. In conclusion, genistein showed a strong immune modulatory effect on the macrophages. © 2013 Elsevier B.V. All rights reserved. Source


Xu J.,University of Strasbourg | Xu J.,Yangzhou University | Houssin R.,University of Strasbourg | Bernard A.,Ecole Centrale Nantes | Caillaud E.,University of Strasbourg
CIRP Journal of Manufacturing Science and Technology | Year: 2013

Manufacturing companies are compelled to innovate through design that is a knowledge-intensive process. However, there lacks a comprehensive knowledge model while considering the specific requirements of innovation in design. In this paper, we propose a systemic model of knowledge for innovation based on our analyses and argumentations, which is characterized by the knowledge content and context from the perspective of systems thinking. By using Unified Modeling Language (UML), the model is implemented and developed into a prototype system of knowledge management for innovation. Then, a simple industrial use case is presented for demonstrating the applicability of our model. Finally, our major contributions and future work about the performance evaluation of knowledge development are discussed. © 2012 CIRP. Source


Hegab I.M.,Suez Canal University | Wei W.,Yangzhou University
Physiology and Behavior | Year: 2014

Predator odors are non-intrusive and naturalistic stressors of high ethological relevance in animals. Upon exposure to a predator or its associated cues, robust physiological and molecular anti-predator defensive strategies are elicited thereby allowing prey species to recognize, avoid and defend against a possible predation threat. In this review, we will discuss the nature of neuroendocrine stress responses upon exposure to predator odors. Predator odors can have a profound effect on the endocrine system, including activation of the hypothalamic-pituitary-adrenal axis, and induction of stress hormones such as corticosterone and adrenocorticotropic hormone. On a neural level, short-term exposure to predator odors leads to induction of the c-fos gene, while induction of δFosB in a different brain region is detected under chronic predation stress. Future research should aim to elucidate the relationships between neuroendocrine and behavioral outputs to gage the different levels of anti-predator responses in prey species. © 2014 Elsevier Inc. Source


Poly(aniline-co-diphenylamine and 5-aminosalicylic acid), i.e., poly(aniline-co-DPA-and 5-ASA), was electrochemically synthesized in a acidic solution containing aniline, diphenylamine (DPA) and 5-aminosalicylic acid (5-ASA) in the presence of N,N-dimethylformamide (DMF), which was deposited on a reduced graphene oxide (RGO)/glassy carbon (GC) electrode to form a poly(aniline-co-DPA and 5-ASA)/RGO/GC electrode. Cyclic voltammetry demonstrated that this electrode can effectively catalyze the reduction of m-nitrophenol in aqueous solutions. Therefore, this electrode can be used to determine m-nitrophenol in aqueous media. The electrode exhibited a linear region of 20-400 μM m-nitrophenol in a phosphate buffer solution of pH 6.5 with a correlation coefficient of 0.9955, a linear region of 20-600 μM m-nitrophenol in a Na2SO4 solution of pH 6.0 with a correlation coefficient of 0.9997, a good repeatability and long working life. © 2015 Elsevier B.V. All rights reserved. Source


Wang B.,University of Technology, Sydney | Park J.,Gyeongsang National University | Su D.,University of Technology, Sydney | Wang C.,Yangzhou University | And 3 more authors.
Journal of Materials Chemistry | Year: 2012

A CoS 2-graphene nanocomposite was prepared by a facile solvothermal method. FESEM and TEM analyses have confirmed that CoS 2 nanoparticles with sizes of 5-15 nm are densely anchored on graphene nanosheets. The as-prepared nanocomposite was electrochemically tested as an electrode material for supercapacitors. The CoS 2-graphene nanocomposite exhibited specific capacitances of 314 F g -1 in the aqueous electrolyte and 141 F g -1 in the organic electrolyte at a current rate of 0.5 A g -1 with excellent cycling stability. The electrochemical performance of the nanocomposite has been significantly improved, compared to bare graphene nanosheets and CoS 2 nanoparticles. This could be credited to the 3D nanoarchitecture, in which CoS 2 nanoparticles were sandwiched between graphene nanosheets, and the additional electrochemical contribution of the decorated CoS 2 nanoparticles. © 2012 The Royal Society of Chemistry. Source


Feng L.G.,Yangzhou University
Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban | Year: 2010

A water culture experiment with controlled dissolved oxygen concentration was conducted to explore the effects of exogenous NO3- on the root function and enzyme activities related to nitrogen metabolism of cherry (Prunun cerasus x P. canescens) seedlings under hypoxia stress. Comparing with the control (7.5 mmol NO3- x L(-1)), treatments 15 and 22.5 mmol NO3- x L(-1) made the materials for plant metabolism abundant, ensured the synthesis of enzyme proteins, increased root activity, maintained root respiration, improved the activities of enzymes related to nitrogen metabolism, such as nitrate reductase (NR), glutamine synthethase (GS), and glutamate dehydrogenase (NADH-GDH) in roots, and thereby, supplied enough energy for root respiration and NAD+ to glycolytic pathway, ensured electron transfer, and avoid ammonium toxicity under hypoxia stress. As a result, the injury of hypoxia stress to cherry plant was alleviated. Applying NO3- at the concentration of 22.5 mmol x L(-1) was more advisable. However, NO3- deficiency (0 mmol x L(-1)) showed opposite results. The above results suggested that applying exogenous NO3- to growth medium could regulate cherry root function and nitrogen metabolism, and antagonize the damage of hypoxia stress on cherry roots. Source


Cheng Y.,Jingdezhen Ceramic Institute | Pan S.,Yangzhou University
Materials Letters | Year: 2013

Nanosized silicalite-2 zeolite was successfully synthesized by steam-assisted dry-gel conversion technique with TBAOH (tetrabutylammonium hydroxide) as structure directing agent and TEOS (tetraethoxysilane) as silica source. The products were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, N2 adsorption and desorption (BET) and Field emission scanning electron microscopy (FESEM). XRD pattern confirmed the crystallization of silicalite-2 obtained at 170 C for 48 h, the diffraction lines of samples were broadened as a result of the decreased in particle size with the content of TBAOH increased. FESEM images showed that the silicalite-2 nanocrystals with a particle size of about 70-90 nm. N2 adsorption and desorption provides manifest evidence that the porosity is fully accessible from the external surface of the zeolite crystal. The vibration bands at around 551.5 and 1229.2 cm-1 indicated the presence of double 5-ring in the pentasil zeolite structure of MEL, values of the 550 cm -1/460 cm-1 band intensity ratios demonstrated that the crystallinity of zeolite closed to 100%. Source


Zhou B.,Nanjing Agricultural University | Liu H.L.,Nanjing Agricultural University | Shi F.X.,Nanjing Agricultural University | Wang J.Y.,Yangzhou University
Animal Genetics | Year: 2010

MicroRNAs (miRNAs) are endogenous non-coding RNAs of ∼22 nucleotides in length that play important roles in multiple biological processes by degrading targeted mRNAs or repressing mRNA translation. To evaluate the roles of miRNA in porcine skeletal muscle, miRNA expression profiles were investigated using longissimus muscle tissue from pigs at embryonic day 90 (E90) and postpartum day 120 (PD120). First, we used previously known miRNA sequences from humans and mice to perform blast searches against the porcine expressed sequence tag (EST) database; 98 new miRNA candidates were identified according to a range of filtering criteria. These miRNA candidates and 73 known miRNAs (miRBase 13.0) from pigs were chosen for porcine miRNA microarray analysis. A total of 16 newly identified miRNAs and 31 previously known miRNAs were detected in porcine skeletal muscle tissues. During later foetal development at E90, miR-1826, miR-26a, miR-199b and let-7 were highly expressed, whilst miR-1a, miR-133a, miR-26a and miR-1826 showed highest abundance during the fast growing stage at PD120. Using the 47 miRNAs detected by the microarray assay, we performed further investigations using the publicly available porcine mRNA database from NCBI and computed potential target hits using the software rnahybrid. This study identified 16 new miRNA candidates, computed potential target hits for 18 miRNA families and determined the miRNA expression profiles in porcine skeletal muscle tissues at different developmental stages. These results provide a valuable resource for investigators interested in post-transcriptional gene regulation in pigs and related animals. © 2010 Stichting International Foundation for Animal Genetics. Source


Qi Z.-M.,U.S. Department of Agriculture | Feng S.-Y.,Yangzhou University | Helmers M.J.,Iowa State University
Pedosphere | Year: 2012

Human health has been potentially threatened by cadmium (Cd) contained in sewage irrigation water. Previous studies of Cd transport in soils were mainly conducted using small soil cores with pH values less than 6. The objectives of this study were to determine the parameters of the convection-dispersion equation (CDE) for Cd transport in relatively larger columns with neutral and alkaline soils, and to investigate the parameters' variability with depth. The soil columns were 50 cm in length and 12.5 cm in diameter. Ceramic suction lysimeters were buried at depths of 2.5, 7.5, 17.5, 27.5, and 37.5 cm to abstract soil solution. Cd concentration in the soil solution samples were subsequently analyzed to obtain breakthrough curves (BTCs). Equilibrium and nonequilibrium models in CXTFIT program were used to estimate parameters of the CDE. The results suggested that both equilibrium and non-equilibrium models performed well in modeling Cd transport. The hydrodynamic dispersion coefficient (D) ranged from 0.18 to 10.70 cm2 h-1, showing large differences among different depths. The retardation factor (Rd) ranged from 25.4 to 54.7 and the standard deviation of Rd value was lower than 30% of the mean value. Precipitation coefficient (Rp) decreased consistently with increasing depth, varying from 1.000 × 10-10 to 0.661 h-1. Sensitivity tests showed that D was less sensitive than Rd. These results would be helpful in understanding the transport and retention of Cd in non-acidic soils. © 2012 Soil Science Society of China. Source


Bao W.B.,Yangzhou University
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2011

Based on the paired full-sib individuals selected from the established resource populations of Sutai pig that were characterized as resistant or sensitive to ETEC F18, Agilent double labeled cDNA microarray was used to identify the gene expression profiles in duodenum on purpose of investigating the genes related to Escherichia coli F18 receptor, which may cause edema disease and post-weaning diarrhea in piglets, as well as exploring the molecular mechanism about the differences involved in two different lineages. The results showed that thirteen differently expressed genes were found in one matched group including sensitive ones with GG genotype comparing with resistant ones with AA genotype at a two-fold filter, where there were 6 up-regulated genes and 7 down-regulated genes. In the other matched group composed of sensitive ones with AG genotype, 4 up-regulated genes and 2 down-regulated genes, 6 in total were screened out. GO analy-sis revealed that the differently expressed genes participated in many biological processes, such as immune response, ex-tracellular region, bacterial binding, response to external stimulus and so on. Meanwhile, these genes were mainly related to the Glycan Biosynthesis and Metabolism and Immune System pathways. Actually, the roles that they may play in edema disease and post-weaning diarrhea need further study and verification. Source


Chen Y.,China Pharmaceutical University | Hui H.,China Pharmaceutical University | Yang H.,China Pharmaceutical University | Zhao K.,China Pharmaceutical University | And 5 more authors.
Blood | Year: 2013

Wogonoside is the main flavonoid component derived from the root of Scutellaria baicalensis Georgi. It is a popular Chinese herbal medicine with the potential to treat hematologic malignancies. In this study, we investigated the anticancer effects of wogonoside in acute myeloid leukemia (AML) cell lines and primary patient-derived AML cells. Wogonoside exerted antiproliferative properties both in vitro and in vivo. Furthermore, it efficiently inhibited the proliferation of U937 and HL-60 cells through the induction of G1 phase arrest and the promotion of differentiation. We also demonstrated that wogonoside significantly increased the transcription of phospholipid scramblase 1 (PLSCR1) due to its influence on the expression of cell cycle- and differentiation-related genes, including the upregulation of p21waf1/cip1 and downregulation of the oncogenic protein c-Myc. Wogonoside also promoted PLSCR1 trafficking into the nucleus and facilitated its binding to the inositol 1,4,5-trisphosphate receptor 1 (IP3R1) promoter, thus increasing the expression of IP3R1. Finally, inhibition of PLSCR1 expression with small interfering RNA partially blocked wogonoside-induced cell cycle arrest and differentiation and disturbed the wogonoside-associated molecular events. The results of this study therefore suggest that wogonoside may represent a therapeutic candidate for the treatment of AML. © 2013 by The American Society of Hematology. Source


Wang K.,CAS Institute of Genetics and Developmental Biology | Tang D.,CAS Institute of Genetics and Developmental Biology | Hong L.,CAS Institute of Genetics and Developmental Biology | Xu W.,CAS Institute of Genetics and Developmental Biology | And 5 more authors.
PLoS Genetics | Year: 2010

Sexual reproduction is essential for the life cycle of most angiosperms. However, pseudovivipary is an important reproductive strategy in some grasses. In this mode of reproduction, asexual propagules are produced in place of sexual reproductive structures. However, the molecular mechanism of pseudovivipary still remains a mystery. In this work, we found three naturally occurring mutants in rice, namely, phoenix (pho), degenerative palea (dep), and abnormal floral organs (afo). Genetic analysis of them indicated that the stable pseudovivipary mutant pho was a double mutant containing both a Mendelian mutation in DEP and a non-Mendelian mutation in AFO. Further map-based cloning and microarray analysis revealed that dep mutant was caused by a genetic alteration in OsMADS15 while afo was caused by an epigenetic mutation in OsMADS1. Thus, OsMADS1 and OsMADS15 are both required to ensure sexual reproduction in rice and mutations of them lead to the switch of reproductive habit from sexual to asexual in rice. For the first time, our results reveal two regulators for sexual and asexual reproduction modes in flowering plants. In addition, our findings also make it possible to manipulate the reproductive strategy of plants, at least in rice. © 2010 Wang et al. Source


Chen Y.,Nanjing Southeast University | Chen Y.,Yangzhou University | Wang C.,Nanjing Southeast University
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2014

We investigate hydrodynamic interaction between two neutrally buoyant circular drops in a confined shear flow based on a computational fluid dynamics simulation using the volume-of-fluid method. The rheological behaviors of interactive drops and the flow regimes are explored with a focus on elucidation of underlying physical mechanisms. We find that two types of drop behaviors during interaction occur, including passing-over motion and reversing motion, which are governed by the competition between the drag of passing flow and the entrainment of reversing flow in matrix fluid. With the increasing confinement, the drop behavior transits from the passing-over motion to reversing motion, because the entrainment of the reversing-flow matrix fluid turns to play the dominant role. The drag of the ambient passing flow is increased by enlarging the initial lateral separation due to the departure of the drop from the reversing flow in matrix fluid, resulting in the emergence of passing-over motion. In particular, a corresponding phase diagram is plotted to quantitatively illustrate the dependence of drop morphologies during interaction on confinement and initial lateral separation. © 2014 American Physical Society. Source


Tang B.-X.,Yangzhou University
Gongcheng Lixue/Engineering Mechanics | Year: 2012

Inspired by the thought of an orthogonal expansion, this paper defines a new concept of a monophyletic random vector, proves the independence of its members, and introduces its application in stochastic analysis. An example calculation shows the effectiveness of the monophyletic analysis method (MAM). By use of the monophyletic random vector, only one basic random variable is needed to express a complicated stochastic system. This method will sharply reduce the difficulties of analysis and calculation, and can be applied in the stochastic analysis based on the correlation theory, such as a random structure, a random field, a stochastic process and a composite stochastic question. After simulating a stochastic system with the use of a monophyletic random vector, the analysis methods corresponding to MAM must be further researched. Source


Liu S.Z.,Kaifeng University | Zhang Y.C.,Yangzhou University | Wang T.X.,Henan Institute of Science and Technology | Yang F.X.,Henan Institute of Science and Technology
Materials Letters | Year: 2012

ZnO 2 hollow nanospheres and nanotubes were synthesized via hydrothermal treatment of ZnO submicron-sized powder in 20 vol.% H 2O 2 aqueous solution at 120 °C for 6 h, and ZnO with similar hollow nanostructures could be obtained by thermal decomposition of the hydrothermally-synthesized ZnO 2 hollow nanospheres and nanotubes in air at 200 °C for 6 h. The possible formation mechanisms of the ZnO 2 and ZnO hollow nanostructures were proposed. The present work pointed out a simple, cost-effective and green way of converting bulk ZnO powder into hollow-nanostructured ZnO 2 and ZnO powders. The as-synthesized hollow-nanostructured ZnO 2 and ZnO powders have promising uses as catalysts, adsorbents and light weight fillers, etc. © 2011 Elsevier B.V. All rights reserved. Source


Rapeseed (Brassica napus L.) is one of the most important economic crops worldwide, and Sclerotinia sclerotiorum is the most dangerous disease that affects its yield greatly. Lipid transfer protein (LTP) has broad-spectrum anti-bacterial and fungal activities. In this study, B. napus was transformed using Agrobacterium tumefaciens harboring the plasmid-containing LTP gene to study its possible capability of increasing plant's resistance. First, we optimized the petiole genetic transformation system by adjusting the days of explants, bacterial concentrations, ratio of hormones, and cultivating condition. Second, we obtained 8 positive plants by PCR analysis of T0 generation. The PCR results of T1 generation were positive, indicating that the LTP gene had been integrated into B. napus. Third, T1 transgenic plants inoculated by detached leaves with mycelia of S. sclerotiorum showed better disease resistance than non-transformants. Oxalic acid belongs to secondary metabolites of S. sclerotiorum, and several studies have demonstrated that the resistance of rapeseed to oxalic acid is significantly consistent with its resistance to S. sclerotiorum. The result from the seed germination assay showed that when T1 seeds were exposed to oxalic acid stress, their germination rate was evidently higher than that of non-transformant seeds. In addition, we measured some physiological changes in T1 plants and control plants under oxalic acid stress. The results showed that T1 transgenic plants had lower malondialdehyde (MDA) content, higher super oxide dismutase (SOD), and peroxidase (POD) activities than non-transformants, whereas disease resistance was related to low MDA content and high SOD and POD activities. Source


Zhang J.,Yangzhou University | Peng A.,Huaihai Institute of Technology
Transactions of Nanjing University of Aeronautics and Astronautics | Year: 2010

Based on analyzing the influences of a slicing scheme on stair-stepping effect, supporting structure, efficiency and deformation, etc., analytical hierarchical process (AHP) combining with fuzzy synthetic evaluation is introduced to make decision in slicing schemes for a processing part. The application in determining the slicing scheme for a computer mouse during prototyping shows that the method increases the rationality during decision-making and improves quality and efficiency for the prototyping part. Source


Comparative proteomics of seed filling between yellow-seeded progeny from somatic hybrids Brassica napus-Sinapis alba and black-seeded parent (B. napus) were taken out using two-dimensional electrophoresis (2-DE). The process indicated distinct differences in 2, 3, 4, 5, 6 weeks after fertilization (WAF) and mature seed. A total of 8 out of the 27 discriminate proteins were identified by mass spectrum analysis and MASCOT comparison, including protein kinase, enolase, triosephosphate isomerase, and dioxygenase. PCR primers contrived for the putative genes were applied for further identification of progenies and both parents, which indicated that spot A3-5 might be the novel protein of intergeneric hybrid, i.e., A5-2 derived from S. alba. Applying these specific primers, this study demonstrates that the new yellow-seeded germplasm is different from the existing yellow seed materials of rapeseed. Source


Huang J.,Yangzhou University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2010

OBJECTIVE: We expressed and purified Campylobacter jejuni flagellin FlaA protein to develop monoclonal antibodies (mAbs) against this protein. METHODS: The C. jejuni flaA gene was amplified and inserted into the expression plasmids, pET30a (+) and pGEX-6p-1. The purified rHis-FlaA protein was used as an immunogen in 8-week-old BALB/c mice, and injected subcutaneously. The purified rGST-FlaA protein used as a detecting antigen for screening mAbs against FlaA was prepared by using a denaturation and renaturation technique. The specificity of mAbs was characterized by Dot-ELISA and Western blot assays. RESULTS: The recombinant expression plasmids, pET30a (+)-flaA and pGEX-6p-1-flaA were obtained. The sizes of the recombinant proteins, rHis-FlaA and rGST-FlaA, were consistent with their predicted size. Specific reaction was found between FlaA positive serum and expressed protein by Western-blot assay, confirming its identification as a Campylobacter jejuni immunogen. Three hybridoma cell lines, designated 2D12, 5A12 and 6A9, secreting mAbs against FlaA were obtained. Their immunoglobulin subclasses were IgG2a, IgG1 and IgG1, respectively. The ELISA titers of the ascites fluid were 1:102 400, 1:102 400 and 1:51 200, respectively. Western blot analysis confirmed that the three mAbs reacted with the rHis-FlaA fusion protein but not the His tag. The Dot-ELISA results demonstrated that the three mAbs only with FlaA and not the tags for the expression vectors. CONCLUSION: The successful preparation of three mAbs specific for the FlaA protein lays the foundation for further study regarding the biological characteristics of FlaA and the pathogenesis of C. jejuni. Source


Chen W.,Anhui Normal University | Luo J.,Central China Normal University | Tang Y.,Yangzhou University
IEEE Transactions on Information Theory | Year: 2015

In this paper, we consider a class of exponential sums from some half quadratic binomials. The exponential sums are proven to be eleven-valued with maximal magnitude (1}/2(q-q) except for the trivial value q. As applications, first, we investigate the autocorrelation and cross-correlation distribution among the sequences in a sequence family. Second, we determine the weight distributions of several classes of linear codes. Some of the dual codes have minimum distance four, which are optimal with respect to the Hamming bound. Our results extend the result by Choi et al. and show that some correlation values in it do not occur. © 2014 IEEE. Source


Yang Y.,Yangzhou University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2012

To clone and express fan operon gene clusters of K99 fimbriae in enterotoxigenic Escherichia coli (ETEC) in vitro, and study the activity of the recombinant E. coli expressing K99 fimbriae. K99 fimbriae gene clusters were amplified by long-PCR method, using the genomic DNA of K99-fimbriae E. coli C8307 as the DNA template. The 5.7Kb PCR products were inserted into expressing vector pBR322 with restriction endonuclease, then positive clones were screened. The positive recombinant plasmid was transformed into non-fimbriae E. coli SE5000 strains, and pBR322 plasmid was also transformed into SE5000 for negative control strain. The recombination E. coli expressing K99 fimbriae was tested with agglutination assay, using monoclonal antibody serum and brush border vesicles from the piglet small intestinal epithelia cells. The expressed fimbriae on the surface of the recombinant E. coli SE5000 were observed by transmissible electromicroscope. Heat extraction method was employed to isolate and purify K99 fimbriae, which was exerted SDS-PAGE, and 18.5 kDa protein band was detected. The mouse sera produced from recombinant fimbriae was used to test K99-fimbriae strains C83907, C83914, C83260 with positive agglutination results, while negative results were found with E. coli contain other kinds of fimbriae. The assays of SDS-PAGE, Western blot, agglutination assay were used to evaluate antigenicity and biologic activity between C83907 and recombinant strain. Adhesion test with HeLa cell line demonstrated the recombinant strain and wild type have the similar adherence ability, and this adhesion can be inhibited with mouse serum containing polyclonal antibody against recombinant K99 fimbriae. This study has laid a good foundation for further study on bioactivity of K99. Source


Li K.-Q.,Nanjing Agricultural University | Xu X.-Y.,Yangzhou University | Huang X.-S.,Nanjing Agricultural University
PLoS ONE | Year: 2016

Drought is a major abiotic stress that affects plant growth, development and productivity. Pear is one of the most important deciduous fruit trees in the world, but the mechanisms of drought tolerance in this plant are still unclear. To better understand the molecular basis regarding drought stress response, RNA-seq was performed on samples collected before and after dehydration in Pyrus betulaefolia. In total, 19,532 differentially expressed genes (DEGs) were identified. These genes were annotated into 144 Gene Ontology (GO) terms and 18 clusters of orthologous groups (COG) involved in 129 Kyoto Encyclopedia of Genes and Genomes (KEGG) defined pathways. These DEGs comprised 49 (26 up-regulated, 23 down-regulated), 248 (166 up-regulated, 82 down-regulated), 3483 (1295 up-regulated, 2188 down-regulated), 1455 (1065 up-regulated, 390 down-regulated) genes from the 1 h, 3 h and 6 h dehydration-treated samples and a 24 h recovery samples, respectively. RNAseq was validated by analyzing the expresson patterns of randomly selected 16 DEGs by quantitative real-time PCR. Photosynthesis, signal transduction, innate immune response, protein phosphorylation, response to water, response to biotic stimulus, and plant hormone signal transduction were the most significantly enriched GO categories amongst the DEGs. A total of 637 transcription factors were shown to be dehydration responsive. In addition, a number of genes involved in the metabolism and signaling of hormones were significantly affected by the dehydration stress. This dataset provides valuable information regarding the Pyrus betulaefolia transcriptome changes in response to dehydration and may promote identification and functional analysis of potential genes that could be used for improving drought tolerance via genetic engineering of non-model, but economically-important, perennial species. Copyright © 2016 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source


The hedgehog (Hh) pathway has been implicated in the pathogenesis of cancer including pancreatic ductal adenocarcinoma (PDAC). Recent studies have suggested that Hh plays an important role in maintaining the cancer stem cell (CSCs) pool. Gemcitabine-resistant pancreatic cancer cells highly express some of the CSCs markers. However, the expression level of Hh members in gemcitabine-resistant pancreatic cancer cells remains unknown. The aim of this study was to verify the expression of HH members, such as Shh, Ptc, SMO and Gli-1 in gemcitabine-resistant PDAC cell lines, and to explore a new strategy to overcome chemoresistance in PDAC. Quantitative real-time RT-PCR (Q-PCR) and western blot were used to evaluate the relative expression level of HH members in SW1990, CFPAC-1 cells and gemcitabine-resistant SW1990, CFPAC-1 cells. The change of cancer stem cell markers and the expression level of HH members before and after cyclopamine treatment was evaluated using flow cytometry and Q-PCR, western blot, respectively. Cell apoptosis after cyclopamine treatment was measured by flow cytometry. CD44, CD133 and the expression level of HH members, including Shh, SMO, Gli-1, were found to be highly expressed in gemcitabine-resistant cells, which were significantly down-regulated by cyclopamine treatment. Flow cytometry analysis showed increased cell apoptosis after cyclopamine treatment. Gemcitabine-resistant pancreatic cancer cells highly express CSCs markers and some of the HH members, and inhibition of HH by cyclopamine is an effective method of reversing gemcitabine resistance in pancreatic cancer. Source


Zhou H.,Yangzhou University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2012

Animal-originated enterotoxigenic Escherichia coli (ETEC) are major pathogens resulting in newborn and young animal diarrhea. Adhesins and enterotoxins, both are essential for the pathogenicity of ETEC, are two major virulent factors of ETEC. Adhesion of animal-originated ETEC fimbrial adhesins (mainly including K88, K99, 987P, F18, F17 and F41) to intestinal epithelial cells is the initial and most important step involved in the ETEC infection. From the 1960s, studies on ETEC fimbrial genes, structure, biosynthesis, regulation of expression, interaction between fimbriae and host receptors have helped to better understand the biology and role of these organelles in pathogenesis. These studies also provide insight into new diagnostic tools and development of vaccines and inhibitors of ETEC colonization. Source


Yin Y.H.,Yangzhou University
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2012

To explore the possibility of transgenic animals by testicular injection, the goat heart-type fatty acid binding protein (H-FABP) expression vector pEGFP-H-FABP was injected into the testis of 6 mice randomly by liposome mediated transfection. By detection of testis slice, sperm fluorescence and sperm DNA PCR, the exogenous gene was expressed in the parental mice. The exogenous gene was expressed at different levels in both the F1 generation mice gave birthed by treated male mice and normal female mice and the F2 generation mice generated by mating F1 could be detected that the exogenous gene expressed at different levels with the positive rates of 4% and 30.23%, respectively. The results suggested that testicu-lar injection, as an effective method to generate transgenic animal, could realize the stable integration of exogenous gene. The amelioration and maturity of testicular injection provides theoretical and practical significance in generation of trans-genic animals and even in the animal trait improvement and breeding. Source


Huo Z.,China Agricultural University | Feng S.,China Agricultural University | Feng S.,Yangzhou University | Kang S.,China Agricultural University | And 3 more authors.
Journal of Hydrology | Year: 2012

Streamflow model including rainfall-runoff and river flow models play an important role in water resources management, especially in arid inland area. Traditional conceptual models have the disadvantage of requirement of spatial variation parameters about the physical characteristics of the catchments. To overcome this difficulty, in this study, several integrated Artificial Neural Networks (ANNs) were presented to estimate monthly river flow, and the models include the semi-distributed forms of ANNs that can explore spatial variation in hydrological process (such as rainfall distribution and evaporation distribution) and no requirement of physical characteristic parameters of the catchments. In an arid inland basin of Northwest, integrated ANNs were developed using hydrological and agricultural data, and its performance was compared with that of lumped ANN and local linear regression model (LLR). Results showed that the integrated ANNs perform well to estimate the monthly streamflow at outlet of mountain with Root Mean Square Error (RMSE) of 0.36×10 7m 3 and Relative Error (RE) of 9%. Similarly, the integrated ANNs can also accurately estimate the monthly river flow downstream of the basin with RMSE of 0.35-0.38×10 7m 3 and RE of 22-27%. When compared with integrated ANNs, the lumped ANN and LLR models have lower precision to simulate monthly streamflow in arid inland basin. Presented integrated ANN models retain the advantages of the semi-distributed models considering the heterogeneity and spatial variation of hydrological factors and the physical characteristics in the catchment, while taking advantage of the potential of ANNs as an effective tool in nonlinear mapping or functional relationship establishment. In contrast to traditional models either in the lumped ANN or in empirical regression forms, the new approach of integration of Artificial Neural Networks has shown great potential in streamflow modeling. © 2011 Elsevier B.V. Source


Wang D.R.,Yangzhou University
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2012

To investigate the safety and feasibility of laparoscopic D2 gastrectomy for advanced gastric cancer. The clinical data of 210 cases of laparoscopic gastrectomy and 180 cases of open gastrectomy for radical (D2) gastrectomy from May 2007 to Dec 2010 were analyzed retrospectively. A total of 206 cases underwent laparoscopic-assisted surgery with 4 conversions. Compared to the open group, the laparoscopic group was associated with less bleeding [(208±38) ml vs. (300±52) ml, P<0.05], quicker postoperative recovery of bowel function [(2.9±0.7) d vs. (3.9±1.8) d, P<0.05], shorter postoperative length of hospital stay[(12.8±6.2) d vs. (15.6±6.8) d, P<0.05], longer operative time [(258±42) min vs. (193±30) min, P<0.05]. The number of lymph node harvested was 20.5±1.9 in the laparoscopic group and 25.8±1.5 in the open group, and the postoperative complication rate was 8.1% (17/201) vs. 8.5% (15/180), and differences were not statistically significant (both P>0.05). The recurrence rate was 2.9% (6/210) and 2.8% (5/180), and the 3-year overall survival rate was 35.6% and 37.8%, the differences were not statistically significant (both P>0.05). Laparoscopic radical gastrectomy for gastric cancer is safe and effective, which can reach the same range of lymph node dissection as open gastric cancer surgery and similar survival rate. Source


Jiang B.,Nanjing Medical University | Li Z.,Nanjing Medical University | Zhang W.,Yangzhou University | Wang H.,Nanjing Medical University | And 7 more authors.
Journal of Gastroenterology | Year: 2014

Background: Aquaporin-3 (AQP3) is a water transporting protein which plays an oncogenic role in several malignant tumors. However, its regulatory mechanism remains elusive to date. In this study, we investigated the microRNA-mediated gene repression mechanism involved in AQP3's role. Methods: The potential microRNAs targeting AQP3 were searched via bioinformatic methods and identified by luciferase reporter assays, microRNA RT-PCR and western blotting. The expression patterns of miR-874 and AQP3 in human gastric cancer (GC) specimens and cell lines were determined by microRNA RT-PCR and western blotting. 5-ethynyl-2′-deoxyuridine, cell migration and invasion assays and tumorigenicity in vivo were adopted to observe the effects of miR-874 depletion or ectopic miR-874 expression on GC cell phenotypes. Cell apoptosis was evaluated by FACS and TUNEL in vitro and in vivo respectively. Results: miR-874 suppressed AQP3 expression by binding to the 3′UTR of AQP3 mRNA in GC cells. miR-874 was significantly down-regulated and reversely correlated with AQP3 protein levels in clinical samples. Analysis of the clinicopathological significance showed that miR-874 and AQP3 were closely correlated with GC characteristics. Functional analyses indicated that ectopic miR-874 expression suppressed the growth, migration, invasion and tumorigenicity of GC cells, whereas miR-874 knockdown promoted these phenotypes. Down-regulation of Bcl-2, MT1-MMP, MMP-2 and MMP-9 and upregulation of caspase-3 activity and Bax were involved in miR-874 inducing cell apoptosis, and inhibiting migration and invasion. Conclusions: These results provide a mechanism by which AQP3 is upregulated, as well as highlight the importance of miR-874 in gastric cancer development and progression. © 2013 Springer. Source


Ding W.M.,Yangzhou University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012

To prepare the mouse polyclonal antibody against human BRDT-NY prokaryotic protein and analyze the expression of BRDT-NY protein in digestive tract tumors. The N-terminal amino acids of BRDT-NY protein was amplified by PCR and cloned into the expression vector pET28a(+). The recombinant plasmid pET28a+-BRDT-NY was transformed into E.coli BL21 and induced to express the recombinant protein with IPTG. We immunized BALB/c mice with the purified BRDT-NY protein for preparing the specific polyclonal antibody. The titer of the antibody was analyzed by ELISA. The expression of BRDT-NY protein in digestive tract tumors was detected by immunohistochemistry. Recombinant human BRDT-NY protein was expressed in BL21 and purified successfully by Ni-affinity chromatography. Two months after the mice were immunized with the purified BRDT-NY protein, we obtained the specific polyclonal antibody of high titer 1:100 000. Immunohistochemical analysis revealed that BRDT-NY protein was highly expressed in digestive tract tumors. The successful preparation of mouse polyclonal antibody against BRDT-NY lays a foundation for the research on the role of BRDT-NY protein in the pathology of human digestive tract tumors. Source


Hilgers R.H.P.,University of Alabama at Birmingham | Xing D.,University of Alabama at Birmingham | Gong K.,University of Alabama at Birmingham | Gong K.,Yangzhou University | And 3 more authors.
American Journal of Physiology - Heart and Circulatory Physiology | Year: 2012

Acute increases in cellular protein O-linked N-acetyl-glucosamine (OGlcNAc) modification (O-GlcNAcylation) have been shown to have protective effects in the heart and vasculature. We hypothesized that D-glucosamine (D-GlcN) and Thiamet-G, two agents that increase protein O-GlcNAcylation via different mechanisms, inhibit TNF-α-induced oxidative stress and vascular dysfunction by suppressing inducible nitric oxide (NO) synthase (iNOS) expression. Rat aortic rings were incubated for 3h at 37°C with D-GlcN or its osmotic control L-glucose (L-Glc) or with Thiamet-G or its vehicle control (H 2O) followed by the addition of TNF-α or vehicle (H 2O) for 21 h. After incubation, rings were mounted in a myograph to assess arterial reactivity. Twenty-four hours of incubation of aortic rings with TNF-α resulted in 1) a hypocontractility to 60 mM K + solution and phenylephrine, 2) blunted endothelium-dependent relaxation responses to ACh and substance P, and 3) unaltered relaxing response to the Ca 2+ ionophore A-23187 and the NO donor sodium nitroprusside compared with aortic rings cultured in the absence of TNF-α. D-GlcN and Thiamet-G pretreatment suppressed the TNF-α-induced hypocontractility and endothelial dysfunction. Total protein OGlcNAc levels were significantly higher in aortic segments treated with D-GlcN or Thiamet-G compared with controls. Expression of iNOS protein was increased in TNF-α-treated rings, and this was attenuated by pretreatment with either D-GlcN or Thiamet-G. Dense immunostaining for nitrotyrosylated proteins was detected in the endothelium and media of the aortic wall, suggesting enhanced peroxynitrite production by iNOS. These findings demonstrate that acute increases in protein O-GlcNAcylation prevent TNF-α-induced vascular dysfunction, at least in part, via suppression of iNOS expression. © 2012 the American Physiological Society. Source


Ye X.-S.,Yangzhou University
Physica C: Superconductivity and its Applications | Year: 2013

Quasiparticle bound states and vortex core contraction effects in iron-based superconductors are studied by solving the Bogoliubov de Gennes (BdG) equations self-consistently including pair coupling effects. We find that the appearance of quasiparticle bound states in the vortex core is controlled not only by the pair coupling effects but also by the inter-orbit coupling strength. We also point out that the rapid vortex core contraction is controlled by quasiparticle interference effects. We suggest that these results deserve more attention in analysis of vortex quasiparticle bound states and vortex core contraction effects found in scanning tunneling microscopy (STM) experiments for different iron-based superconductors. © 2012 Elsevier B.V. All rights reserved. Source


Shi H.,Arizona State University | Shi H.,Yangzhou University | Wang S.,Arizona State University | Curtiss III R.,Arizona State University
Clinical and Vaccine Immunology | Year: 2013

We developed regulated delayed attenuation strategies for Salmonella vaccine vectors. In this study, we evaluated the combination of these strategies in recombinant attenuated Salmonella enterica serovar Typhi and Salmonella enterica serovar Typhimurium vaccine vectors with similar genetic backgrounds in vitro and in vivo. Our goal is to develop a vaccine to prevent Streptococcus pneumoniae infection in newborns; thus, all strains delivered a pneumococcal antigen PspA and the impact of maternal antibodies was evaluated. The results showed that all strains with the regulated delayed attenuated phenotype (RDAP) displayed an invasive ability stronger than that of the S. Typhi vaccine strain, Ty21a, but weaker than that of their corresponding wild-type parental strains. The survival curves of different RDAP vaccine vectors in vitro and in vivo exhibited diverse regulated delayed attenuation kinetics, which was different from S. Typhi Ty21a and the wild-type parental strains. Under the influence of maternal antibody, the persistence of the S. Typhimurium RDAP strain displayed a regulated delayed attenuation trend in nasal lymphoid tissue (NALT), lung, and Peyer's patches, while the persistence of S. Typhi RDAP strains followed the curve only in NALT. The bacterial loads of S. Typhi RDAP strains were lower in NALT, lung, and Peyer's patches in mice born to immune mothers than in those born to naive mothers. In accordance with these results, RDAP vaccine strains induced high titers of IgG antibodies against PspA and against Salmonella lipopolysaccharides. Immunization of mothers with S. Typhi RDAP strains enhanced the level of vaginal mucosal IgA, gamma interferon (IFN-γ), and interleukin 4 (IL-4) and resulted in a higher level of protection against S. pneumoniae challenge. Copyright © 2013, American Society for Microbiology. All Rights Reserved. Source


Chen Y.,Yangzhou University | Chen Y.,Nanjing Southeast University | Liu X.,Nanjing Southeast University | Shi M.,Nanjing Southeast University
Applied Physics Letters | Year: 2013

Hydrodynamic behaviors of double emulsion droplets in shear flow, both deformation and breakup, are investigated numerically. We find that the inner drop is deformed in a uniform vortical flow interior to the outer drop under steady state deformation conditions and provides an additional deformation resistance for the integral droplet especially when its volume fraction is large. In particular, we report four types of breakup modes via three mechanisms (i.e., necking, end pinching, and capillary instability), respectively, and present the corresponding phase diagrams to describe the breakup criteria (critical capillary numbers) and morphologies. © 2013 American Institute of Physics. Source


Wang H.,CAS Shanghai Institute of Materia Medica | Jiang Y.,CAS Shanghai Institute of Materia Medica | Peng H.,CAS Shanghai Institute of Materia Medica | Chen Y.,CAS Shanghai Institute of Materia Medica | And 2 more authors.
Advanced Drug Delivery Reviews | Year: 2015

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression. Because of significant changes in their expression in cancer, miRNAs are believed to be key factors in cancer genetics and to have potential as anticancer drugs. However, the delivery of miRNAs is limited by many barriers, such as low cellular uptake, immunogenicity, renal clearance, degradation by nucleases, elimination by phagocytic immune cells, poor endosomal release, and untoward side effects. Nonviral delivery systems have been developed to overcome these obstacles. In this review, we provide insights into the development of non-viral synthetic miRNA vectors and the promise of miRNA-based anticancer therapies, including therapeutic applications of miRNAs, challenges of vector design to overcome the delivery obstacles, and the development of miRNA delivery systems for cancer therapy. Additionally, we highlight some representative examples that give a glimpse into the current trends into the design and application of efficient synthetic systems for miRNA delivery. Overall, a better understanding of the rational design of miRNA delivery systems will promote their translation into effective clinical treatments. © 2014 Elsevier B.V. Source


Wu Z.X.,Yangzhou University
Applied Mechanics and Materials | Year: 2013

A gradientless method for two-dimensional shape optimization is developed based on the magnitude of local relative-stress difference along the design boundary. The design boundary is modeled by using cubic splines, which are determined by a number of control points. The optimal shape of a design boundary with constant stress is achieved iteratively by moving control points consecutively (correspondingly, changing the shape of the design boundary) by an amount depending on the relative-stress difference between two neighboring boundary points. The key feature of the optimization method is that no arbitrary threshold stress is required. The result quality in terms of accuracy and efficiency are tested and discussed with several finite element analysis examples. © (2013) Trans Tech Publications, Switzerland. Source


Target For the lotus roots have caring characteristics on inventions of obesity and preventions of“Three Height”, which compared the test on the absorption function of lotus roots absorbing cholesterol and fatty acids after cooking ,provide the basis for reasonable edible method and develop the special adsorbent. Method The lotus roots were steamed, boiled, fried and microwaved, then tested and compared the changes of the adsorption of cholesterol and fatty acid, studied on the influence factors of adsorption, and got the processing method of high adsorbability. Results ① With the extension of adsorption time, the amount of cholesterol absorbed by all samples surges, which peaks after 1-1.5h. While as more lotus roots are used ,the amount of cholesterol absorbed declines, which compared absorption by different methods: microwaving > poaching>steaming>frying. ② The lotus roots absorbed saturated fatty acids>the lotus roots absorbed unsaturated fatty acids,which compared absorption by different methods: steaming> poaching>microwaving>frying.Conclusion In order to treat disease of heart head blood-vessel and ensure to play a role in absorption and excretion on cholesterol and saturated fatty acids after eating ,Lotus roots are promoted to use cooking recipes of microwaving,steaming and poaching instead of frying. © (2014) Trans Tech Publications, Switzerland. Source


Jang Y.,Yangzhou University
Journal of Convergence Information Technology | Year: 2011

To better embody the evolutionary characteristics, namely dynamic variety and smooth transition of network users, the method of network user identification based on evolutionary K-means clustering algorithm is proposed. Every clustering result of the sequence reflects the classification information of network user at a special period. Furthermore, the information of historical data is considered and used to adjust the clustering result of the current data. Then, the current clustering result is smoothed and will not shift dramatically from the previous clustering. The experiment results show that the method of evolutionary K-means can improve the accuracy of network user behavior analysis and the efficiency of network management. Source


Wang T.,Henan Institute of Science and Technology | Zhang Y.,Yangzhou University | Ding T.,Yongcheng Vocational College
Materials Letters | Year: 2014

A one-step in situ solvothermal method was proposed for the synthesis of SnIn4S8/TiO2 nanocomposite with 6.5 mol% SnIn4S8, using SnCl4·5H2O, InCl3·4H2O, thiourea, tetrabutyl titanate and absolute ethanol as the source materials. The structure, composition, BET specific surface area and optical property of the as-synthesized SnIn 4S8/TiO2 nanocomposite were characterized by powder X-ray diffraction, energy dispersive X-ray spectroscopy, field emission scanning electron microscopy, N2 adsorption and UV-vis diffuse reflectance spectra. The photocatalytic activity of the as-synthesized SnIn 4S8/TiO2 nanocomposite was tested in the reduction of aqueous Cr(VI) under visible-light (λ>420 nm) irradiation, and compared with those of nanostructured SnIn4S 8 and TiO2. The photocatalytic results demonstrated that the as-synthesized SnIn4S8/TiO2 nanocomposite exhibited much higher visible-light-activated photocatalytic activity than SnIn4S8 and TiO2 nanoparticles. The reasons accounting for the photocatalytic results were also discussed. © 2014 Elsevier B.V. Source


Wang J.,Yangzhou University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2014

To develop a novel H5 subtype avian influenza vaccine candidate based on flagellin. HA1-2-fljB was amplified through splicing hemagglutinin gene fragment HA1-2 of A/Goose/Jiangsu/1/2000 H5N1 and Salmonella typhimurium II phase flagellin fljB gene by overlap PCR, and then the gene was inserted to pET32a(+) to construct the recombinant plasmid pET32a-HA1-2-fljB. The recombinant plasmid was transformed into E.coli BL21 (DE3) and was induced with IPTG. The expression of the fusion protein was identified by SDS-PAGE and Western blotting. The biological activity of the fusion protein was evaluated by stimulating HEK293-TLR5 cells. The recombinant plasmid pET32a-HA1-2-fljB was constructed. The fusion protein, whose relative molecule weight was about 100 000, had a good immunoreactivity. TLR5 bioassay demonstrated the flagellin part of the fusion protein could be recognized by TLR5 of HEK293-TLR5 cell line to secrete a higher level of IL-8 when compared with HA1-2 protein (P<0.01). The flagellin-based fusion protein HA1-2-fljB with TLR5 bioactivity was expressed correctly, and the results would establish a basis for the further research on avian influenza virus H5 vaccine. Source


Kruglova O.,Catholic University of Leuven | Demeyer P.-J.,Catholic University of Leuven | Zhong K.,Catholic University of Leuven | Zhou Y.,Catholic University of Leuven | And 3 more authors.
Soft Matter | Year: 2013

In nature the spontaneous formation of ordered structures from molecules, called self-assembly, is a very common process occurring in inorganic matter and living organisms. It is driven by atoms, molecules, particles, granular matter, etc. trying to reach the lowest possible energy state while interacting with each other. Deeper understanding of the subtleties of such interactions will allow mimicking of this kind of behaviour to build custom structures from synthetic molecules. This review attempts to cover the existing techniques for directed self-assembly that are currently used for colloidal crystal growth with a brief explanation of the interactions involved in each technique. It provides examples of the fundamental phenomena occurring in photonic crystals that, in the future, can be exploited in various applications. © 2013 The Royal Society of Chemistry. Source


Fang Y.,Nanjing Southeast University | Fang Y.,Yangzhou University | Ma X.,Nanjing Southeast University
Dianli Xitong Zidonghua/Automation of Electric Power Systems | Year: 2011

A topology of coupled inductor double boost inverter (CIDBI) is proposed based on the photovoltaic micro-inverter module system. The operation principle and control strategy of CIDBI are analyzed in detail and the gain expression from DC side to AC side is deduced. It can generate an AC output voltage much larger than the DC input one, depending on the proper setup of instantaneous duty cycle and turn ratio of the coupled inductors. Firstly, based on a given driving signal series, the operational principle of CIDBI is discussed. Secondly, the voltage stress of switches in CIDBI and the relationship between input and output are analyzed. The gain is approximately proportional to the duty cycle when the duty cycle is around 0.5 and the sinusoidal pulse width modulation (SPWM) is applicable to this novel inverter. Finally, the proposed CIDBI serves as a grid-connected inverter in a photovoltaic generation system. Experimental results show that the CIDBI can competently implement the single-stage grid-connected generation of photovoltaic system with a low level total harmonic distortion of current and high efficiency, without traditional transformer and DC/DC converter. © 2011 State Grid Electric Power Research Institute Press. Source


Jin H.,Nanjing Southeast University | Jiang Y.,Yangzhou University | Wei Q.,Nanjing Southeast University | Wang B.,Nanjing Southeast University | Ma G.,Nanjing Southeast University
Disability and Rehabilitation | Year: 2012

Purpose: To evaluate the effect of aerobic cycling training with lower limb weights on cardiovascular fitness (peak VO 2) and walking ability in chronic stroke survivors, and to investigate the relationship between changes in these parameters. Methods: 133 Chinese patients with chronic hemiparetic stroke (mean age 58 years) were randomized to either 8-week (5×/week) aerobic cycling training with lower limb weights group (n = 68) or a low-intensity overground walking group (n = 65). Peak VO 2, 6-minute walk distance (6MWD), knee muscle strength, balance and spasticity were measured before and after intervention. Results: Cycling training increased peak VO 2 (24% vs. 3%, p < 0.001), 6MWD (2.7% vs. 0.5%, p < 0.001), paretic (11% vs. 1.6%, p < 0.001) and nonparetic knee strength (16% vs. 1.0%, p < 0.001). In the cycling group, percent changes in peak VO 2 were positively associated with those in paretic (r = 0.491, p < 0.001) and nonparetic knee strength (r = 0.432, p < 0.001). Increased 6MWD correlated significantly with improved balance, spasticity and paretic knee strength by the stepwise regression analysis (r = 0.342, p = 0.004), but not fitness gains. Conclusions: The enhanced cardiovascular fitness after aerobic cycling training in Chinese patients with chronic stroke is not associated with the increased walking ability. Unparallel improvements in these parameters related different determinants may have implications for intervention strategy. © 2012 Informa UK, Ltd. Source


Rong J.-H.,Jinan University | Ji L.-J.,Yangzhou University | Yang Z.-Z.,CAS Institute of Chemistry
Chinese Journal of Polymer Science (English Edition) | Year: 2013

This review summarizes recent progress of the ordered macroporous composites with the opals and inverse opal structure. Synthesis and performance of the composites are emphasized. Composition of the ordered composites is tunable ranging within metal, metal alloy, metal oxide, polymer, carbon and hydrogel. The ordered structure gives brilliant color effects, which is useful for sensors and photonic crystals. The interconnected macroporous structure provides easiness for mass transportation and species culturing. © 2013 Chinese Chemical Society, Institute of Chemistry, Chinese Academy of Sciences and Springer-Verlag Berlin Heidelberg. Source


Cosnier S.,CNRS Molecular Chemistry Department | Shan D.,CNRS Molecular Chemistry Department | Shan D.,Yangzhou University | Ding S.-N.,CNRS Molecular Chemistry Department | Ding S.-N.,Nanjing University
Electrochemistry Communications | Year: 2010

We report on the easy and fast immobilization of glucose oxidase (GOD) and laccase by mechanical compression with graphite particles to form disc electrodes. The electrical wiring of GOD and laccase was efficiently carried out by their co-inclusion with ferrocene (Fc) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonate) diammonium salt (ABTS) respectively. A glucose/air compartment-less biofuel cell was constructed based on the association of GOD-ferrocene-graphite disc and laccase-ABTS - graphite disc electrodes as bioanode and biocathode respectively. Such biofuel cell yielded a power density of 23 μW cm-2 at 0.33 V as well as an open-circuit voltage and a short-circuit current of 0.63 V and 166 μA, respectively. © 2009 Elsevier B.V. All rights reserved. Source


Wang J.,University of California at Davis | Wang J.,Yangzhou University | Yang G.-X.,University of California at Davis | Tsuneyama K.,University of Toyama | And 3 more authors.
Seminars in Liver Disease | Year: 2014

Within the last decade, several mouse models that manifest characteristic features of primary biliary cirrhosis (PBC) with antimitochondrial antibodies (AMAs) and immune-mediated biliary duct pathology have been reported. Here, the authors discuss the current findings on two spontaneous (nonobese diabetic autoimmune biliary disease [NOD.ABD] and dominant negative transforming growth factor-β receptor II [dnTGFβRII]) and two induced (chemical xenobiotics and microbial immunization) models of PBC. These models exhibit the serological, immunological, and histopathological features of human PBC. From these animal models, it is evident that the etiology of PBC is multifactorial and requires both specific genetic predispositions and environmental insults (either xenobiotic chemicals or microbial), which lead to the breaking of tolerance and eventually liver pathology. Human PBC is likely orchestrated by multiple factors and hence no single model can fully mimic the immunopathophysiology of human PBC. Nevertheless, knowledge gained from these models has greatly advanced our understanding of the major immunological pathways as well as the etiology of PBC. Copyright © 2014 by Thieme Medical Publishers, Inc. Source


He L.,Yangzhou University
Applied Mechanics and Materials | Year: 2013

In rural waterworks automatic control application and development situation are discussed in this paper, aiming at the characteristics of rural waterworks, the design principles of automatic control system are put forward, and the rural waterworks automatic control system model and unit control technology solutions are analyzed, in order to provide scientific and effective means for rural water construction, and promote modernization operation and management level of rural water supply project. © (2013) Trans Tech Publications, Switzerland. Source


Yang C.,Yangzhou University
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi | Year: 2013

When we try to establish the gene recombinant yeast cell to screen the androgenic endocrine disruptors, the key procedure is the androgen receptor (AR) expression in the yeast cell. For this purpose, we obtained the GPD (glyceraldehyde-3-phosphote dehydrogenase) promoter from the yeast genosome of W303-1A using PCR system and inserting it into Swa I and BamH I sites of pYestrp2. The new constructed vector was named pGPD. The V5 epitope tag DNA with a 5'-BamH I and a 3'-EcoR I sticky end was cloned into the corresponding site of the pGPD vector to yield the vector of pGPDV5. The 2 723 bp full length AR ORF amplified by PCR from pcDNA3.1/AR was fused to V5 epitope tag DNA in pGPDV5 to give the AR yeast expression vector of pGPDV5/AR. This fused vector was transformed into the yeast cell (W303-1A). Western blot was used to detect the V5 fused protein of AR, in the protocol of which the primary monoclonal antibody (IgG(2a)) of mouse anti-V5 and the polyclonal secondary antibody of goat anti-mouse (IgG) linked to horseradish peroxidase (HRP) were used to detect the specific protein in the given sample of the transformed yeast extract. The result showed that the fused protein of AR was expressed successfully in the yeast cell. Source


Chu H.J.,Yangzhou University | Chu H.J.,University of Akron | Pan E.,University of Akron | Wang J.,Los Alamos National Laboratory | Beyerlein I.J.,Los Alamos National Laboratory
International Journal of Solids and Structures | Year: 2011

Dislocations and the elastic fields they induce in anisotropic elastic crystals are basic for understanding and modeling the mechanical properties of crystalline solids. Unlike previous solutions that provide the strain and/or stress fields induced by dislocation loops, in this paper, we develop, for the first time, an approach to solve the more fundamental problem - the anisotropic elastic dislocation displacement field. By applying the point-force Greens function for a three-dimensional anisotropic elastic material, the elastic displacement induced by a dislocation of polygonal shape is derived in terms of a simple line integral. It is shown that the singularities in the integrand of this integral are all removable. The proposed expression is applied to calculate the elastic displacements of dislocations of two different fundamental shapes, i.e. triangular and hexagonal. The results show that the displacement jump across the dislocation loop surface exactly equals the assigned Burgers vector, demonstrating that the proposed approach is accurate. The dislocation-induced displacement contours are also presented, which could be used as benchmarks for future numerical studies. © 2011 Elsevier Ltd. All rights reserved. Source


Purpose: In view of the lotus root has significant antioxidant properties but if improperly cooked easily destroyed, compared with traditional cooking method, and discusses the applicability of microwave processing and establish the optimum processing conditions. Method: By setting different microwave power and heating time, handling fresh-cut lotus root vacuum packaging products with microwave, measured flavonoids, total phenolic content and DPPH clearance, and synchronization researched the traditional cook methods cooking, steaming and stir-frying, comprehensive comparison the antioxidant effect of all kinds of processed products. Results: ①Under following conditions microwave power ≥60 P, processing time≤6min, flavonoids and total phenol content were high, DPPH clearance was bigger also. ②Cooking 6min,water volume between 500-1000mL, flavonoids and total phenol contents was high, but change with the further increased water volume, the contents decrease, DPPH clearance was relatively stable, when confirmed water volume 1500mL, cooking 2-4min, flavonoids content slightly ascending, then changed into degressive changes, the change of DPPH clearance was similar. Total phenol content was decrease progressively along with extending of the cooking time. ③Handing under steaming 15min, three index of the products were high voltage products > atmospheric products, the latter's steamed time should be less than 20 min. ④Making products in oil capacity 25mL or less 3 min, with the increase of oil capacity, flavonoids, total phenolic content and DPPH clearance were progressive increase. ⑤Lotus root food antioxidant activity: steamed food < cooked food < microwave food < fried food. Conclusion: In protecting lotus root antioxidant health care function in different cropping system, microwave is a kind of superior to boiling, steaming, and pressure cooking method and second only to oil fry. © (2014) Trans Tech Publications, Switzerland. Source


Luo J.,Yangzhou University | Luo J.,University of Bergen | Helleseth T.,University of Bergen
IEEE Transactions on Information Theory | Year: 2011

Constant composition codes are codes where the frequency distribution of the elements in a codeword is the same for all codewords. In this paper, three classes of constant composition codes are constructed. These codes are subcodes of cyclic codes which have few weights occurring among the codewords. The new codes are excellent asymptotically compared to the previously best known constant composition codes. © 2011 IEEE. Source


Zhang W.,Yangzhou University | Fang J.-A.,Donghua University | Cui W.,Donghua University
Journal of the Franklin Institute | Year: 2013

This paper concerns the stability analysis problem for stochastic delayed switched genetic regulatory networks (GRNs) with both stable and unstable subsystems. By employing the piecewise Lyapunov functional method combined with the average dwell time approach, we show that if the average dwell time is chosen sufficiently large and the derivative of the Lyapunov-like function for unstable subsystems is bounded by certain kind of continuous function, then exponential stability criteria of a desired degree are guaranteed. The derived results show that the minimal average dwell time is proportional to the time delays. Finally, an example is given to illustrate the effectiveness of the derived results. © 2013 The Franklin Institute. Source


Zhu P.T.,Yangzhou University
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2011

To study the correlation between the serum levels of phospholipid transfer protein (PLTP), cholesteryl ester transfer protein (CETP), and Chinese medical syndrome types of coronary heart disease (CHD) patients, thus probing a new pathway for the objectivity of CHD syndrome typing and developing therapeutic drugs. 201 patients with CHD confirmed by coronary angiography were selected. The comprehensive analysis database by the four examination methods was established using generally accepted standard for Chinese medical syndrome typing. Twenty healthy subjects were randomly recruited as the control group. Serum samples were separated from venous blood. The serum activities of PLTP and CETP were assayed by ELISA. The triglyceride (TG) content was determined using acetic acetone coloring method. Contents of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were determined using precipitation floating enzyme couplet method. Serum levels of PLTP, CETP, and TC of CHD patients of all Chinese medical syndrome types were significantly higher than those of the control group, showing statistical difference (P<0.05, P<0.01). Statistical difference existed in serum levels of TG, HDL-C, and LDL-C of all Chinese medical syndrome types when compared with the control group (P<0.05, P<0.01). Statistical difference existed in serum levels of PLTP between the sthenia in superficiality groups of all Chinese medical syndrome types and the asthenia in origin groups of all Chinese medical syndrome types (P< 0.05). Statistical difference existed in serum levels of TG, HDL-C, and LDL-C between the sthenia in superficiality groups of all Chinese medical syndrome types and the asthenia in origin groups of all Chinese medical syndrome types (P<0.05, P<0.01). The serum PLTP levels of CHD patients of sthenia in superficiality significantly increased. Changes in serum lipids was more significant in CHD patients of the sthenia in superficiality syndrome than in those of the asthenia in origin syndrome. Source


Liu J.-L.,Yangzhou University
Tamkang Journal of Mathematics | Year: 2011

The object of the present paper is to derive an univalent condition for a family of integral operators. Source


Shen Q.,Nanjing University of Aeronautics and Astronautics | Shen Q.,Yangzhou University | Jiang B.,Nanjing University of Aeronautics and Astronautics | Cocquempot V.,Lille University of Science and Technology
IEEE Transactions on Fuzzy Systems | Year: 2012

This paper addresses the problem of fault-tolerant control for Takagi-Sugeno (T-S) fuzzy systems with actuator faults. First, a general actuator fault model is proposed, which integrates time-varying bias faults and time-varying gain faults. Then, sliding-mode observers (SMOs) are designed to provide a bank of residuals for fault detection and isolation. Based on Lyapunov stability theory, a novel fault-diagnostic algorithm is proposed to estimate the actuator fault, which removes the classical assumption that the time derivative of the output errors should be known as in some existing work. Further, a novel fault-estimation observer is designed. Utilizing the estimated actuator fault, an accommodation scheme is proposed to compensate for the effect of the fault. In addition, a sufficient condition for the existence of SMOs is derived according to Lyapunov stability theory. Finally, simulation results of a near-space hypersonic vehicle are presented to demonstrate the efficiency of the proposed approach. © 1993-2012 IEEE. Source


Zhang X.,Hong Kong Polytechnic University | Zhang X.,Yangzhou University | Xin J.,Hong Kong Polytechnic University | Ding F.,Hong Kong Polytechnic University
Nanoscale | Year: 2013

The edge of two dimensional (2D) graphene, as the surface of a three dimensional (3D) crystal, plays a crucial role in the determination of its physical, electronic and chemical properties and thus has been extensively studied recently. In this review, we summarize the recent advances in the study of graphene edges, including edge formation energy, edge reconstruction, method of graphene edge synthesis and the recent progress on metal-passivated graphene edges and the role of edges in graphene CVD growth. We expect this review to provide a guideline for readers to gain a clear picture of graphene edges from several aspects, especially the catalyst-passivated graphene edges and their role in graphene CVD growth. © The Royal Society of Chemistry 2013. Source


Zou M.,China Pharmaceutical University | Lu N.,China Pharmaceutical University | Hu C.,China Pharmaceutical University | Liu W.,Nanjing Medical University | And 6 more authors.
Cellular Signalling | Year: 2012

Autophagy is a tightly-regulated catabolic process that involves the degradation of intracellular components via lysosomes. Although the pivotal role of autophagy in cell growth, development, and homeostasis has been well understood, its function in cancer prevention and intervention remains to be delineated. The aim of this study was to investigate the function and mechanism of autophagy induced by oroxylin A, a natural mono-flavonoid extracted from Scutellariae radix. We found for the first time that oroxylin A induced Beclin 1-mediated autophagy in human hepatocellular carcinoma HepG2 cells. Time-lapse video microscopy and western blotting studies showed that treatment of cells with 80 μM oroxylin A resulted in the conversion of water soluble MAP-LC3 (LC3-I) to the lipidated and autophagosome-associated form (LC3-II) after 12. hours; then autophagosome-lysosome fusion and lysosome degradation after 24. hours was required in oroxylin A-mediated cell death. This induction was associated with the suppressing of PI3K-PTEN-Akt-mTOR signaling pathway by oroxylin A. Our results also showed that autophagy took place before noticeable apoptosis can be observed. It was further demonstrated that oroxylin A-triggered autophagy contributed to cell death using over-expression of autophagy-related gene (Atg5 and Atg7) and inhibition of autophagy by siBeclin 1 and 3-methyladenine (3-MA). In vivo study, oroxylin A inhibited xenograft tumor growth and induced obvious autophagy in tumors. Taken together, we conclude that oroxylin A exhibits autophagy-mediated antitumor activity in a dose and time-dependent manner in vivo and in vitro. These findings define and support a novel function of autophagy in promoting death of hepatocellular carcinoma cells. © 2012 Elsevier Inc. Source


Guo X.,University of North Carolina at Chapel Hill | Guo X.,Yangzhou University | Huang L.,University of North Carolina at Chapel Hill
Accounts of Chemical Research | Year: 2012

Gene therapy has long been regarded a promising treatment for many diseases, whether acquired (such as AIDS or cancer) or inherited through a genetic disorder. A drug based on a nucleic acid, however, must be delivered to the interior of the target cell while surviving an array of biological defenses honed by evolution. Successful gene therapy is thus dependent on the development of an efficient delivery vector.Researchers have pursued two major vehicles for gene delivery: viral and nonviral (synthetic) vectors. Although viral vectors currently offer greater efficiency, nonviral vectors, which are typically based on cationic lipids or polymers, are preferred because of safety concerns with viral vectors. So far, nonviral vectors can readily transfect cells in culture, but efficient nanomedicines remain far removed from the clinic. Overcoming the obstacles associated with nonviral vectors to improve the delivery efficiency and therapeutic effect of nucleic acids is thus an active area of current research. The difficulties are manifold, including the strong interaction of cationic delivery vehicles with blood components, uptake by the reticuloendothelial system (RES), toxicity, and managing the targeting ability of the carriers with respect to the cells of interest.Modifying the surface with poly(ethylene glycol), that is, PEGylation, is the predominant method used to reduce the binding of plasma proteins to nonviral vectors and minimize clearance by the RES after intravenous administration. Nanoparticles that are not rapidly cleared from the circulation accumulate in the tumors because of the enhanced permeability and retention effect, and the targeting ligands attached to the distal end of the PEGylated components allow binding to the receptors on the target cell surface. Neutral and anionic liposomes have been also developed for systemic delivery of nucleic acids in experimental animal models. Other approaches include (i) designing and synthesizing novel cationic lipids and polymers, (ii) chemically coupling the nucleic acid to peptides, targeting ligands, polymers, or environmentally sensitive moieties, and (iii) utilizing inorganic nanoparticles in nucleic acid delivery.Recently, the different classes of nonviral vectors appear to be converging, and the ability to combine features of different classes of nonviral vectors in a single strategy has emerged. With the strengths of several approaches working in concert, more hurdles associated with efficient nucleic acid delivery might therefore be overcome.In this Account, we focus on these novel nonviral vectors, which are classified as multifunctional hybrid nucleic acid vectors, novel membrane/core nanoparticles for nucleic acid delivery, and ultrasound-responsive nucleic acid vectors. We highlight systemic delivery studies and consider the future prospects for nucleic acid delivery. A better understanding of the fate of the nanoparticles inside the cell and of the interactions between the parts of hybrid particles should lead to a delivery system suitable for clinical use. We also underscore the value of sustained release of a nucleic acid in this endeavor; making vectors targeted to cells with sustained release in vivo should provide an interesting research challenge. © 2011 American Chemical Society. Source


Shen Q.,Yangzhou University | Shi P.,University of Adelaide | Shi P.,Victoria University of Melbourne
Automatica | Year: 2015

This paper investigates the distributed consensus tracking problems of multi-agent systems on undirected graph with a fixed topology. Each follower is assumed to be in strict-feedback form with unknown state-dependent controlling effects. A distributed robust adaptive neural networks-based control scheme is designed to guarantee the consensus output tracking errors between the followers and the leader are cooperatively semi-globally uniformly ultimately bounded. Command filtered backstepping technique is extended to the consensus tracking control problems, which avoids the classical "explosion of complexity" problem in standard backstepping design and removes the assumption that the first n derivatives of the leader's output should be known. The function approximation technique using neural networks is employed to compensate for unknown functions induced from the controller design procedure. Stability analysis and parameter convergence of the proposed algorithm are conducted based on algebraic graph theory and Lyapunov theory. © 2014 Elsevier Ltd. All rights reserved. Source


Wang Y.,Nanjing Medical University | Guo Q.,Yangzhou University | Zhao Y.,Nanjing Medical University | Chen J.,Nanjing Medical University | And 3 more authors.
Oncology Letters | Year: 2014

Long non-coding RNAs (lncRNAs) are novel regulators in cancer biology. BRAF-activated lncRNA (BANCR) is overexpressed in melanoma and has a potential functional role in melanoma cell migration. However, little is known about the role of BANCR in the development of papillary thyroid carcinoma (PTC). In the present study, BANCR expression was examined in six pairs of PTC and matched adjacent normal tissues. The results revealed that BANCR levels were significantly higher in the PTC tissues and PTC IHH-4 cells compared with the normal controls. Knockdown of BANCR in the IHH-4 cells inhibited proliferation and increased apoptosis of the cells in vitro. Further investigation of the underlying mechanisms revealed that BANCR markedly activated autophagy. Overexpression of BANCR inhibited apoptosis in the IHH-4 cells, whereas inhibition of autophagy stimulated apoptosis in the BANCR-overexpressed cells. BANCR overexpression also increased cell proliferation and the inhibition of autophagy abrogated BANCR overexpression-induced cell proliferation. In addition, the overexpression of BANCR resulted in an increase in the ratio of LC3-II/LC3-I, a marker for autophagy, while the knockdown of BANCR decreased the ratio of LC3-II/LC3-I. These results revealed that BANCR expression levels are upregulated in PTC. Additionally, BANCR increases PTC cell proliferation, which could activate autophagy. © 2014, Spandidos Publications. All rights reserved. Source


Zhou F.-X.,Lanzhou University of Technology | Zhou F.-X.,CAS Lanzhou Cold and Arid Regions Environmental and Engineering Research Institute | Li S.-R.,Lanzhou University of Technology | Li S.-R.,Yangzhou University | Lai Y.-M.,CAS Lanzhou Cold and Arid Regions Environmental and Engineering Research Institute
Journal of Sound and Vibration | Year: 2011

The generalized coupled thermoelasticity based on the LordShulman theory is considered to study the transient thermoelastic response of functionally graded rectangular plates. The state equations of functionally graded rectangular plate subjected to time-dependent thermal loads were established by using of state space approach, in which three displacement components, three stress components, the temperature and the heat flux were chosen as state variables. By giving simply supported boundary conditions and assuming that the material properties of the plate have an exponential law distribution along the thickness-coordinate, the equations were solved by the numerical Laplace transformations and shooting methods for transient thermal responses of a three dimensional functionally graded rectangular plate due to a thermal shock on its top surface. Effects of the volume fraction distributions of material constituents on the thermal responses, including the temperature change, the displacement and the stresses distributions were investigated. © 2011 Elsevier Ltd. All rights reserved. Source


Linquist B.A.,University of California at Davis | Liu L.,Yangzhou University | van Kessel C.,University of California at Davis | van Groenigen K.J.,Northern Arizona University | van Groenigen K.J.,Trinity College Dublin
Field Crops Research | Year: 2013

Nitrogen is deficient in most soils and is applied in the greatest quantities of all nutrients. Given its high potential for loss, efficient fertilizer N management has both economic and environmental consequences. Enhanced efficiency nitrogen fertilizers (EENF) have been developed to decrease N losses and improve N use efficiency. However, studies evaluating the effectiveness of EENF products in rice systems show mixed results. The objective of this meta-analysis was to quantify the benefits of EENF (i.e. nitrification and urease inhibitors, neem, and slow release fertilizers) in terms of yield and N uptake and to determine under what conditions EENF are most effective. The analysis included 32 field studies (178 observations) for the effects of EENF on crop yield and 14 studies (82 observations) on N uptake. Overall, the use of EENF led to a 5.7% (95% CI = 3.9-7.7%) increase in yield and an 8.0% (95% CI = 5.2-10.7%) increase in N uptake. Soil pH (pH of dry soil) had a significant impact on EENF effectiveness. In acidic soils (pH ≤ 6.0) the application of EENF did not significantly affect yield or N uptake; however the yield response to EENF increased to 10.2% (95% CI = 5.3-16.6%) in alkaline soils (pH ≥ 8.0). There was no difference among the classes of EENF when separated by their mode of action (i.e. urease inhibitors, nitrification inhibitors or slow release). When EENF products were analyzed separately, NBPT [N-(n-butyl) phosphoric triamide] and neem proved effective in increasing yield, while PPD (phenyl phosphorodiamidate) and DCD (dicyandiamide) were not effective. The EENF effectiveness was not dependent on N rate, method of first N application (incorporated, surface applied, or applied into water), timing of first N application in relation to a permanent flood being established, and how water was managed during the season (permanent flood vs. intermittent wet and dry). Overall, this meta-analysis suggests that certain EENF products can increase yield and N uptake but the average increase is modest. © 2013 Elsevier B.V. Source


Ding S.-N.,Nanjing Southeast University | Cosnier S.,CNRS Molecular Chemistry Department | Shan D.,Yangzhou University | Sun Y.-M.,Nanjing Southeast University | Wang Y.,Nanjing Southeast University
Electrochemistry Communications | Year: 2010

Elelctrogenerated chemiluminescence (ECL) of electropolymerized films based on [(2,2′-bipyridyl)(4-(2-pyrrol-1-ylethyl)-4′-methyl-2,2′- bipyridyl)2]ruthenium (II) was firstly investigated in both organic and aqueous solution. The ECL behaviors have been explained by two typical mechanisms, namely, redox-cycling type and oxidative-reduction type. For the former, no co-reactant was required and for the latter, tripropylamine (TPA) and (NH4)2C2O4 were selected as co-reactants in the organic and aqueous system, respectively. © 2010 Elsevier B.V. All rights reserved. Source


Du H.,Nanjing Medical University | Zhang X.,Yangzhou University | Du M.,Nanjing Medical University | Guo N.,Nanjing Medical University | And 5 more authors.
Scientific Reports | Year: 2014

Previous studies have reported that the Asp1104His polymorphism in Xeroderma Pigmentosum complementation group G (XPG) was associated with the susceptibility to colorectal cancer (CRC), although the results were inconsistent. This study was aim to investigate whether there existed an association betweenXPG Asp1104His polymorphism and CRC risk in the Chinese population, and a further meta-analysis was performed to consolidate the results. We found thatXPG Asp1104His polymorphism was associated with a significantly increased CRC risk (dominant model: His/His + Asp/His vs. Asp/Asp, adjusted OR = 1.39, 95% CI = 1.14-1.69). Stratification analysis by clinical characteristics indicated that the His/His + Asp/His genotypes were associated with increased CRC susceptibility in patients with moderately differentiated grade, but not in poorly and well differentiated grade. Furthermore, a total of 5 eligible studies, including 2,649 CRC cases and 2,848 controls, were recruited for the meta-analysis. We identified that the meta-analysis reported a similar result in dominant model (OR = 1.35; 95% CI = 1.20-1.51). Especially, when stratified by ethnicity, an evidently increased risk was identified in the Asian population. In conclusion, our findings suggest that XPG Asp1104His polymorphism may increase the susceptibility of CRC, especially in Asian populations. Source


Chen X.,China Agricultural University | Cui Z.,China Agricultural University | Fan M.,Stanford University | Vitousek P.,Chinese Academy of Agricultural Sciences | And 28 more authors.
Nature | Year: 2014

Agriculture faces great challenges to ensure global food security by increasing yields while reducing environmental costs1,2. Here we address this challenge by conducting a total of 153 site-year field experiments covering the main agro-ecological areas for rice, wheat and maize production in China. As et of integrated soil-crop systemmanagement practices based ona modern understanding of crop ecophysiology and soil biogeochemistry increases average yields for rice, wheat and maize from 7.2 million grams per hectare (Mg ha-1), 7.2Mg ha-1 and 10.5Mgha-1 to 8.5Mgha-1, 8.9Mgha-1 and 14.2Mgha-1, respectively, without any increase in nitrogen fertilizer. Model simulation and life-cycle assessment3 show that reactive nitrogen losses and greenhouse gasemissions are reduced substantially by integrated soil-crop system management. If farmers in China could achieve average grain yields equivalent to 80%of this treatment by 2030, over the sameplanting area as in 2012, total production of rice, wheat and maize in China would be more than enough to meet the demand for direct human consumption and a substantially increased demand for animal feed, while decreasing the environmental costs of intensive agriculture. © 2014 Macmillan Publishers Limited. All rights reserved. Source


Gu J.,Wageningen University | Gu J.,Yangzhou University | Yin X.,Wageningen University | Stomph T.-J.,Wageningen University | Struik P.C.,Wageningen University
Plant, Cell and Environment | Year: 2014

Rice productivity can be limited by available photosynthetic assimilates from leaves. However, the lack of significant correlation between crop yield and leaf photosynthetic rate (A) is noted frequently. Engineering for improved leaf photosynthesis has been argued to yield little increase in crop productivity because of complicated constraints and feedback mechanisms when moving up from leaf to crop level. Here we examined the extent to which natural genetic variation in A can contribute to increasing rice productivity. Using the mechanistic model GECROS, we analysed the impact of genetic variation in A on crop biomass production, based on the quantitative trait loci for various photosynthetic components within a rice introgression line population. We showed that genetic variation in A of 25% can be scaled up equally to crop level, resulting in an increase in biomass of 22-29% across different locations and years. This was probably because the genetic variation in A resulted not only from Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase)-limited photosynthesis but also from electron transport-limited photosynthesis; as a result, photosynthetic rates could be improved for both light-saturated and light-limited leaves in the canopy. Rice productivity could be significantly improved by mining the natural variation in existing germ-plasm, especially the variation in parameters determining light-limited photosynthesis. © 2013 John Wiley & Sons Ltd. Source


Wang Z.,Brunel University | Wang Z.,Donghua University | Liu Y.,Yangzhou University | Wei G.,Donghua University | Liu X.,Brunel University
Automatica | Year: 2010

This paper is concerned with the state feedback control problem for a class of discrete-time stochastic systems involving sector nonlinearities and mixed time-delays. The mixed time-delays comprise both discrete and distributed delays, and the sector nonlinearities appear in the system states and all delayed states. The distributed time-delays in the discrete-time domain are first defined and then a special matrix inequality is developed to handle the distributed time-delays within an algebraic framework. An effective linear matrix inequality (LMI) approach is proposed to design the state feedback controllers such that, for all admissible nonlinearities and time-delays, the overall closed-loop system is asymptotically stable in the mean square sense. Sufficient conditions are established for the nonlinear stochastic time-delay systems to be asymptotically stable in the mean square sense, and then the explicit expression of the desired controller gains is derived. A numerical example is provided to show the usefulness and effectiveness of the proposed design method. © 2009 Elsevier Ltd. All rights reserved. Source


Xu L.,Yangzhou University
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013

To investigate the clinical features, the CT, MRI features and therapeutic strategy of nasal neuroendocrine carcinoma (NEC). A retrospective study was carried out on 6 cases of NEC. 2 were female and 4 were male. Routine preoperative CT and MRI examination were performed. In one case,the tumor had invaded the brain and then was treated in department of oncology. Five cases were undertaken tumor resection under nasal endoscope, and combined with postoperative chemoradiation therapy. The postoperative pathological report of 1 cases was large cell type, the remaining 4 cases were small cell type. One case treated in department of oncology had lived for 2 month with tumor in his body. One cases relapsed and died at 4 month after postoperation, 1 case was lost and 3 case did not relapse till now. NEC of paranasal sinuses is malignant, but early detection, early diagnosis and treatment can improve the prognosis of this disease. For limited lesion of NEC, nasal endoscopic operation is an effective, safe and minimally injury method. Source


N-doped ZnO micropolyhedrons were fabricated by calcining the mixture of commercial ZnO (analyticalgrade) and NH4NO3at 600C for 1.5 h, in which NH4NO3was utilized as the nitrogen source. The structure,composition, BET specific surface area and optical properties of N-doped ZnO sample were characterizedby X-ray diffraction, X-ray photoelectron spectroscopy, wavelength dispersive X-ray fluorescence spec-troscopy, field emission scanning electron microscopy, high resolution transmission electron microscopy,N2adsorption-desorption isotherms, and UV-vis diffuse reflectance spectroscopy. The photocatalyticresults demonstrated that the as-synthesized N-doped ZnO microcrystals possessed much higher pho-tocatalytic activity than N-doped TiO2(which was synthesized by calcining the mixture of P25 TiO2and NH4NO3at 600C for 1.5 h) and commercial pure ZnO in the decomposition of formaldehyde undervisible-light (λ > 420 nm) irradiation. The present work suggests that NH4NO3is a promising nitrogensource for one-step calcination synthesis of microcrystalline N-doped ZnO, which can be applied as avisible-light-activated photocatalyst in efficient utilization of solar energy for treating formaldehydewastewater. © 2014 Elsevier B.V. All rights reserved. Source


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