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Cao J.-F.,The First Affiliated Hospital of Liaoning Medical College | Pang D.-B.,The First Affiliated Hospital of Liaoning Medical College | Ye W.-J.,Yangzhou Hospital of Traditional Chinese Medicine
International Eye Science | Year: 2013

AIM: To investigate the expression of the hypoxia-inducible factor-1α (HIF-1α) and the protein products of its target gene vascular endothelial growth factor (VEGF) in proliferative diabetic retinopathy (PDR) epiretinal membranes. METHODS: Totally 26 patients who have been obtained epiretinal membranes (PDRV-VI) were performed pars-plana vitrectomy. They were removed the epiretinal membrane intraoperation and to observe HIF-1α, VEGF and expression of vascular endothelial marker CD34 in epiretinal membranes through immunohistochemical method. RESULTS: Vascular endothelial cells of PDR expressed HIF-1α in 24 cases (92.3%) and VEGF in 15 cases (57.7%) in membranes, respectively. There were significant correlations between the numbers of blood vessels expressing CD34 and HIF-1α (r=0.556, P=0.028). There were significant correlations between the numbers of blood vessels expressing CD34 and VEGF (r=0.745, P=0.001). CONCLUSION: HIF-1α and VEGF may play an important role in the pathogenesis of PDR. Source


E M.,U.S. Center for Disease Control and Prevention | E M.,Shandong University | Yu S.,Shandong University | Dou J.,U.S. Center for Disease Control and Prevention | And 5 more authors.
Neurological Sciences | Year: 2016

The purpose of this study is to examine the association between alcohol consumption and amyotrophic lateral sclerosis. Published literature on the association between alcohol consumption and amyotrophic lateral sclerosis was retrieved from the PubMed and Embase databases. Two authors independently extracted the data. The quality of the identified studies was evaluated according to the Newcastle–Ottawa scale. Subgroup and sensitivity analyses were performed and publication bias was assessed. Five articles, including one cohort study and seven case–control studies, and a total of 431,943 participants, were identified. The odds ratio for the association between alcohol consumption and amyotrophic lateral sclerosis was 0.57 (95 % confidence interval 0.51–0.64). Subgroup and sensitivity analyses confirmed the result. Evidence for publication bias was detected. Alcohol consumption reduced the risk of developing amyotrophic lateral sclerosis compared with non-drinking. Alcohol, therefore, has a potentially neuroprotective effect on the development of amyotrophic lateral sclerosis. © 2016 Springer-Verlag Italia Source


Chen L.-L.,Yangzhou Hospital of Traditional Chinese Medicine | Bu P.,Yangzhou University
World Chinese Journal of Digestology | Year: 2015

AIM: To investigate the relationship between gastroesophageal reflux disease (GERD) and the changes of brain gut peptides and the relationship between the changes of brain gut peptides and TCM syndrome types. METHODS: One hundred and seventy-eight GERD patients were included, and they were divided into five groups based on syndrome types: stagnation of the liver and stomach, Qi stagnation and phlegm blockade, phlegm stagnation and blood stasis, Qi deficiency, and Qi stagnation and blood stasis. Plasma levels of brain gut peptides including 5-hydroxytryptamine (5-HT), somatostatin (SS), vasoactive intestinal peptide (VIP), endothelin (ET), and motilin (MTL) were determined. RESULTS: Of 178 patients with GERD, the incidence of stagnation of the liver and stomach was significantly higher in patients with reflux esophagitis (RE) than in those with nonerosive reflux disease (NERD) (P < 0.05), and the incidences of phlegm stagnation and blood stasis and Qi deficiency were significantly higher in the NERD group than in the RE group (P < 0.05). GERD patients had abnormal levels of multiple brain gut peptides. Plasma levels of VIP in both patients with RE and those with NERD were significantly higher than that in the control group (P < 0.05). Plasma VIP levels in patients were related with MTL levels and TCM syndrome types; plasma VIP levels were significantly higher in patients with stagnation of the liver and stomach than in those with Qi deficiency (P < 0.05), and in patients with Qi stagnation and blood stasis than in those with phlegm stagnation and blood stasis, Qi deficiency, and Qi stagnation and blood stasis (P < 0.05). CONCLUSION: Plasma VIP and MTL may be involved in the occurrence and development of GERD. Stagnation of the liver and stomach is more common in GERD. Plasma VIP and MTL levels are different from traditional Chinese medicine syndrome. © 2015 Baishideng Publishing Group Inc. All rights reserved. Source


Dai X.-J.,Yangzhou Hospital of Traditional Chinese Medicine | Dai X.-J.,Nanjing University of Traditional Chinese Medicine | Lu J.-W.,Jiangsu Cancer Hospital | Wang Z.,Nanjing University of Traditional Chinese Medicine | And 3 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2014

OBJECTIVE: To investigate whether Ginsenoside Rg3 immune-nanoemulsion (VRIN) can inhibit growth and lymphangiogenesis of gastric cancer in vivo. METHODS: A gastric cancer model was developed by surgical orthotopic implantation of red fluorescent protein (RFP)-expressing NUGC-4 tumor in nude mice. Thirty-two Balb/c nude mice were randomly divided into 4 groups: saline treated group, 5-FU treated group (20 mg/kg), high dose(1 mg/kg)VRIN treated group and low dose(0.1 mg/kg) VRIN treated group. At the end of the experiment, all mice were sacrificed and open fluorescent images of tumors and metastasis expressing RFP were acquired, and the tumor was removed from each mouse. The size of transplantation tumor and tumor weight were measured; gene and protein expressions of VEGF-C in tumor tissues were detected by Real time-PCR and immunohistochemistry; lymphatic microvessel density (LMVD) in tumors was measured with immunohistochemistry. RESULTS: At the end of the experiment, open RFP imaging demonstrated that tumor weight decreased by 72.9%, 14.5% and 69.2% in high dosage VRIN treated group (1 mg/kg), low dosage VRIN treated group (0.1 mg/kg) and 5-FU treated group (20 mg/kg). Compared to saline treated group, tumor weight decreased significantly in high dosage VRIN treated group and 5-FU treated group (both P values<0.001). The metastasis ratio of lymph nodes in saline treated group, 5-FU treated group, high dosage VRIN treated group (1 mg/kg) and low dosage VRIN treated group (0.1 mg/kg) were 87.5%(7/8), 50.0%(4/8), 12.5%(1/8) and 37.5%(3/8) respectively. Compared with saline treated group, lymph node metastasis was significantly inhibited (P=0.01) in high dosage (1 mg/kg) VRIN treated group. LMVD in tumor tissues treated with saline treated group, 5-FU treated group, high dose VRIN treated group (1 mg/kg) were 9.29±2.06, 6.42±1.38, 4.25±1.08, respectively. LMVD in tumor tissues treated with high dose VRIN (1 mg/kg) and 5-FU (20 mg/kg) were both statistically significantly lower than that in the saline treated group(F=20.895, P<0.001). In regard to tumor VEGF-C expressions in saline groups(0.269±0.051) and VRIN at the dose of 1 mg/kg (0.190±0.059), the difference between VRIN treated group and saline treated group was statistically significant (P=0.014). VEGF-C mRNA expressions in saline treated group, 5-FU (at 20 mg/kg) treated group and VRIN (at the dose of 1 mg/kg) treated group were 1.05±0.19, 0.62±0.25 and 0.49±0.19 respectively. Compared with the group treated with saline, the mRNA expression of VEGF-C were significantly lower in the group treated with VRIN at the dose of 1 mg/kg and in 5-FU treated group (F=8.190, P=0.002). CONCLUSIONS: VRIN can inhibit the growth and lymphatic metastasis of human gastric cancer cell transplantation tumor in nude mice. It inhibits tumor lymphangiogenesis through down-regulating VEGF-C expressions. ©, 2014, The Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved. Source


Chen L.-L.,Yangzhou Hospital of Traditional Chinese Medicine | Bu P.,Yangzhou Hospital of Traditional Chinese Medicine | Fang X.-H.,Yangzhou Hospital of Traditional Chinese Medicine
World Chinese Journal of Digestology | Year: 2016

AIM: To investigate the relationship between plasma brain gut peptides, traditional Chinese medicine (TCM) syndrome and the course of gastroesophageal reflux disease (GERD). METHODS: One hundred and sventy-eight GERD patients were included. They had five TCM syndromes: liver stomach heat, gas exchange resistance, phlegm stagnation and blood stasis, Qi deficiency, and Qi stagnation and blood stasis. Plasma levels of brain gut peptides including 5-hydroxytryptamine, somatostatin, vasoactive intestinal peptide, endothelin, and motilin were detected. RESULTS: In the 178 patients with GERD, the proportion of patients with liver stomach heat syndrome was significantly higher in the reflux esophagitis (RE) group than in the non-erosive gastroesophageal reflux disease (NERD) group (P<0.05), while the proportions of patients with phlegm stagnation and blood stasis syndrome and Qi deficiency syndrome were significantly higher in the NERD group than in the RE group (P<0.05). Of the 178 GERD patients, 73 (41%) had a disease course<1 year, 72 (40.5%) had a disease course between 1 and 5 years, and 33 (18.5%) had a course>5 years. The proportion of patients with a course>5 years was significantly higher in the NERD group than in the RE group (P<0.05). The proportion of patients with a course<1 year or between 1 and 5 years in patients with liver stomach heat syndrome was significantly higher that of patients with a course>5 years (P<0.05), the proportion of patients with a course between 1-5 years or>5 years in patients with Qi deficiency syndrome was significantly higher than that of patients with a course<1 year (P<0.05), and the proportion of patients with a course>5 years in patients with phlegm stagnation and blood stasis was significantly higher than that of patients with a course less than 1 year (P < 0.05). Motilin levels were significantly lower in patients with a course>5 years than in patients with a course<1 year (P<0.05), and there was significant differences between the three groups (P<0.05); 5-hydroxytryptamine and endothelin levels were higher in patients with a course>5 years than in patients with a course between 1-5 years, but the difference was not significant. CONCLUSION: The course of GERD patients with different TCM syndromes is different. The course of NERD patients is more likely to increase. GERD patients with a long course of disease often show Qi deficiency syndrome, liver stomach heat syndrome, and phlegm stagnation and blood stasis. Plasma 5-hydroxytryptamine, endothelin and motilin levels may be related to the prolongation of GERD. © 2016 Baishideng Publishing Group Inc. All rights reserved. Source

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