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Liu Y.,Nanjing Medical University | Zhang Q.,Nanjing Medical University | Ren C.,Medical laboratory | Ding Y.,Yangzhou First Peoples Hospital | And 5 more authors.
Journal of Biomedical Research | Year: 2012

MET tyrosine kinase and its ligand, hepatocyte growth factor (HGF), play a pivotal role in the activties of tumor cells. A germline missense variant in exon 2 of the MET gene, N375S (rs33917957 A>G), may alter the binding affinity of MET for HGF and thus modify the risk of tumorigenesis. In this study, we performed a case-control study to assess the association between N375S and gastric cancer risk in 1,681 gastric cancer cases and 1,858 cancer-free controls. Logistic regression analysis was applied to estimate crude and adjusted odds ratios (ORs) and 95% confidence intervals (CIs) for the associations between genotypes and gastric cancer risk. We found that MET N375S variant genotypes (NS/SS) were associated with a significantly decreased risk of gastric cancer (OR = 0.78, 95% CI = 0.63-0.96, P = 0.021) compared with the wildtype homozygote (NN). The finding indicates that this germline variant in MET may decrease gastric cancer susceptibility in Han Chinese. © 2012 The Editorial Board of Journal of Biomedical Research. Source


Wang J.,Philips | Guan M.,Yangzhou First Peoples Hospital | Yamada K.,University of Washington | Hippe D.S.,University of Washington | And 5 more authors.
PLoS ONE | Year: 2016

Objectives: Simultaneous Non-contrast Angiography and intraPlaque hemorrhage (SNAP) technique was recently proposed for joint MRA and intraplaque hemorrhage (IPH) imaging. The purpose of this study is to validate SNAP's MRA performance in patients with suspected intracranial artery disease. Methods: SNAP and time-of-flight (TOF) techniques with matched field of view and resolution were applied on 15 patients with suspected intracranial artery disease. Both techniques were evaluated based on their detection of luminal stenosis of bilateral middle cerebral arteries (MCA) and the delineation of smallest visible branches (SVB) of the MCA. Statistical analysis was conducted on the artery level. Results: The SNAP MRA was found to provide similar stenosis detection performance when compared with TOF (Cohen's κ 0.79; 95% Confidence Interval: 0.56-0.99). For the SVB comparison, SNAP was found to provide significantly better small artery delineation than TOF (p = 0.017). Inter-reader reproducibility for both measurements on SNAP was over 0.7. SNAP also detected IPH lesions on 13% of the patients. Conclusions: The SNAP technique's MRA performance was optimized and compared against TOF for intracranial artery atherosclerosis imaging and was found to provide comparable stenosis detection accuracy. Along with its IPH detection capability, SNAP holds the potential to become a first-line screening tool for high risk intracranial atherosclerosis disease evaluation. © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source


Guo Q.F.,University of Sichuan | Zhang J.,Yangzhou First Peoples Hospital | Li D.X.,University of Sichuan | Zhao Q.,University of Sichuan | And 3 more authors.
Science of Advanced Materials | Year: 2014

In this study, the cationic microspheres were designed by oil-in water emulsion/solvent evaporation technique based on the degradable PEA, which was synthesized by L-lactide and low molecular weight branched polyethlenimine (PEI, Mn = 1800). The properties of microspheres were investigated by size distribution measurement and scanning electron microscopic (SEM), which revealed narrow size distribution, irregular pore distribution throughout microspheres, surface rough and hollow. The adsorption property of the cationic microspheres for bilirubin was mainly investigated, and effects of various factors, such as pH value, ionic intensity, the influence of temperature, and human serum albumin (HSA), were studied. These results showed that HSA and NaCl could prevent from the adsorption of PEA cationic microsphere for bilirubin. In the given temperature range, rising temperature can fasten adsorptivity velocity and enchance absorbability of PEA cationic. The pH value of the medium affects the adsorption capacity greatly, as in the nearly neutral solutions (pH 7.4), the PEA microspheres have the stronger adsorption ability for blilirubin. On the basis of results of the present study, we suggest that PEA microspheres delivery system may help in development of safe, effective strategy for the treatment of hyperbilirubinemic conditions in model animals. © 2014 by American Scientific Publishers. © 2014 by American Scientific Publishers. Source


Gan W.,Nanjing University | Zhou M.,Yangzhou First Peoples Hospital | Xiang Z.,Gothenburg University | Han X.,Nanjing University | Li D.,Nanjing University
International Journal of Environmental Research and Public Health | Year: 2015

The xenoestrogens nonylphenol (NP) and bisphenol A (BPA) are regarded as endocrine disrupting chemicals (EDCs) which have widespread occurrence in our daily life. In the present study, the purpose was to analyze the combined effects of NP and BPA on the human prostate epithelial cell line RWPE-1 using two mathematical models based on the Loewe additivity (LA) theory and the Bliss independence (BI) theory. RWPE-1 cells were treated with NP (0.01–100 μM) and BPA (1–5000 μM) in either a single or a combined format. A cell viability assay and lactate dehydrogenase (LDH) leakage rate assay were employed as endpoints. As predicted by the two models and based on the cell viability assay, significant synergism between NP and BPA were observed. However, based on the LDH assay, the trends were reversed. Given that environmental contaminants are frequently encountered simultaneously, these data indicated that there were potential interactions between NP and BPA, and the combined effects of the chemical mixture might be stronger than the additive values of individual chemicals combined, which should be taken into consideration for the risk assessment of EDCs. © 2015, by the authors. Source


Zhang H.,Nanjing Medical University | Jin G.,Nanjing Medical University | Li H.,Nanjing Medical University | Ren C.,Medical laboratory | And 7 more authors.
Carcinogenesis | Year: 2011

Two recent genome-wide association studies reported significant associations of genetic variants at 1q22, 10q23 and 20p13 with gastric cancer (GC) risk in Chinese populations. However, these findings have not been confirmed in other independent studies. Here, we performed an independent case-control study in a Chinese population by genotyping three loci (rs4072037A>G at 1q22, rs2274223A>G at 10q23 and rs13042395C>T at 20p13) in 1681 GC cases and 1858 controls. We found that rs4072037 at 1q22 and rs2274223 at 10q23 were significantly associated with risk of GC with per allele odds ratio (OR) of 0.72 [95% confidence interval (CI): 0.63-0.81; P 5 2.98 3 1027] and 1.42 (95% CI: 1.27-1.58;P 5 9.68 3 10210), respectively. The association was more prominent for rs2274223 in female (OR 5 1.86, 95% CI: 1.49-2.32) and gastric cardia adenocarcinoma (GCA) (OR 5 1.71, 95% CI: 1.49-1.95). Furthermore, we combined the two single-nucleotide polymorphisms to evaluate the joint effect and found that the GC risk significantly increased with the number of risk allele increasing with a trend P value of 6.66 3 10-16, and individuals with four risk alleles had a 3.28-fold (95% CI: 1.75-6.13) risk of GC compared with those having no risk alleles. However, no significant association was detected between rs13042395 at 20p13 and GC risk (OR 5 1.04, 95% CI: 0.94-1.15; P 5 0.452). In conclusion, our results indicate that genetic variants at 1q22 and 10q23 but not 20p13 may serve as candidate markers for GC susceptibility in the Chinese population. © The Author 2011. Published by Oxford University Press. Source

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