Shanxi, China
Shanxi, China

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Zhang D.,Shanxi Datong University | Wang C.,Yangquan Normal College | Wang C.,China Research Institute of Daily Chemical Industry | Wana G.,China Research Institute of Daily Chemical Industry
Tenside, Surfactants, Detergents | Year: 2012

A new tetrasiloxane-tailed dicephalic surfactant was synthesized. The structures of intermediate and product were confirmed by infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy. Surface activity and wetting ability of the surfactant were also investigated. The results show that the prepared surfactant exhibited excellent surface activity reducing the equilibrium surface tension of water to a minimum value of approximate 21.8 mN • m -1. © Carl Hanser Publisher, Munich.


Mao X.,Harbin Medical University | Miao S.,Harbin Medical University | He H.,Harbin Medical University | Miao S.,Yangquan Normal College | And 8 more authors.
Tumor Biology | Year: 2014

Krüppel-like factor 5 (KLF5), a zinc fingercontaining transcription factor, is involved in important biological processes including cell transformation, proliferation, and carcinogenesis. However, its clinical significance has remained largely unknown in laryngeal cancer. Here, specimens from 144 patients with laryngeal tumors were investigated by immunohistochemical staining for KLF5, integrinlinked kinase (ILK), and E-cadherin expressions. A clinicopathological study revealed that the KLF5 expression level in tumor cells was significantly correlated with lymph node metastasis (P <0.05) and local recurrence (P <0.05). In addition, KLF5, ILK, and E-cadherin (epithelial-mesenchymal transition (EMT) biomarker) expressions were correlated with each other. These findings suggest that KLF5 may be an epithelial- mesenchymal transition-associated biomarker in human laryngeal carcinomas and play important roles in the progression of laryngeal carcinomas. KLF5 immunoreactivity is therefore considered a potential lymph node metastasis and recurrence factor in human laryngeal cancers. In addition, the KLF5-mediated pathway is a potential target for elimination of laryngeal cancer in the future.


Miao S.,Harbin Medical University | Mao X.,Harbin Medical University | Pei R.,Harbin Medical University | Miao S.,Yangquan Normal College | And 6 more authors.
International Journal of Biological Macromolecules | Year: 2013

In our previous study, a potential antitumor polysaccharide (LSPc1) was screened from the fruiting bodies of Lepista sordida. However, its molecular mechanism of cell death induction on Hep-2 human laryngocarcinoma cells has still not been determined. The present study evaluated the anticancer efficacy and associated mechanisms of LSPc1 on Hep-2 cells in vitro. We found that LSPc1-induced inhibition of cell proliferation was associated with an increase in G2/M-phase arrest at 48. h. Typical morphological and biochemical features of apoptosis were also observed in LSPc1-treated cells. Furthermore, LSPc1 led a loss of mitochondrial membrane potential (δΨm), increased the ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2, induced cytochrome c release, and increased the activity of caspase-3 and -9, which altogether account for apoptotic cell death. Taken together, our study suggests that intrinsic mitochondrial caspase dependent pathway plays a very important role in LSPc1-induced cancer apoptosis and these results provided strong experimental evidence for the use of LSPc1 as a potential therapeutic agent in cancer for laryngocarcinoma. © 2013 Elsevier B.V.


Miao S.,Harbin Medical University | Mao X.,Harbin Medical University | Pei R.,Harbin Medical University | Miao S.,Yangquan Normal College | And 6 more authors.
International Journal of Biological Macromolecules | Year: 2013

Four water-soluble polysaccharides (LSPa1, LSPb1, LSPb2 and LSPc1) extracted from the fruiting bodies of Lepista sordida were prepared by DEAE cellulose-52 anion-exchange and Sepharose 6 Fast Flow gel-permeation chromatography. LSPa1 was composed of Glc, Man and Gal with a molar ratio of 3.2: 1.3: 0.6. LSPb1, LSPb2 and LSPc1 were composed of Glc, Man, Gal, Rha and Ara in the proportions of 2.6: 2.1: 0.9: 0.6: 0.3, 1.2: 1.0: 2.3: 0.6: 0.5, 0.2: 0.3: 2.3: 0.3: 2.0, respectively. The high-performance gel-permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of four polysaccharides were approximately 156, 134, 96 and 57. kDa, respectively. MTT and lactate dehydrogenase (LDH) assays indicated that three acidic polysaccharides, namely LSPb1, LSPb2 and LSPc1, possessed more potent antiproliferative effect on human laryngocarcinoma Hep-2 cells than neutral polysaccharide LSPa1 in a dose- and time-dependent manner and the effect increased in the order of LSPa1. <. LSPb2. <. LSPb1. <. LSPc1, which is the same as their uronic acid content. Furthermore the tumor volume and tumor weight were also obviously suppressed after two weeks' treatment of LSPc1 to mice engrafted with Hep-2 cancer cells. Results of these studies demonstrated that the polysaccharide LSPc1 had a potential application as natural antitumor drugs. © 2013 Elsevier B.V.

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