Chen Y.,Guangzhou University |
Lin H.,Yangjiang Peoples Hospital |
Zhang Z.,Guangzhou University |
Lin Y.,Guangdong Academy of Medical science |
And 13 more authors.
Experimental and Toxicologic Pathology
Cyclosporine, tacrolimus and sirolimus are commonly used in renal transplant recipients to prevent rejection. Various adverse effects of these agents on the multiple organ system have been reported clinically. However, animal studies are necessary to determine and compare these effects on individual organ given the presence of multiple confounding factors and multi-pharmacy in clinical settings. In a physiologically and clinically relevant rat model of unilateral nephrectomy, the long-term impacts of commonly used immunosuppressants at doses equivalent to the therapeutic levels used for post-renal transplant patients on hepatic function and histological changes of the liver were examined. Cyclosporine induced significant hepatocellular injury, impairment of synthetic function of the liver, hyperbilirubinemia and cholestasis, and dyslipidemia accompanied by profound histological changes of hepatic structures on both light and electron microscopic examinations. On the other hand, neither tacrolimus nor sirolimus developed any hepatotoxic effects except for more remarkable dyslipidemia was observed in animals treated with sirolimus. Our study indicates that long-term administration of commonly used immunosuppressants has various impacts on biochemical parameters as well as histological alterations of the liver even at therapeutic levels. These data may therefore provide useful information for judicious selection of immunosuppressive agents based on different clinical settings. © 2014 Elsevier GmbH. Source
Liu D.-G.,Yangjiang Peoples Hospital |
Wei Z.-C.,Sun Yat Sen University |
Cai D.-Z.,Southern Medical University |
Zheng J.,Jiangmen Central Hospital |
And 2 more authors.
Chinese Journal of Tissue Engineering Research
Background: Autogenous bone graft is the best way to treat bone defects, but its limited sources and donor site complications restrain its clinical application. Therefore, to develop a substitute material has been a hotspot in the orthopedics. Objective: To investigate the feasibility and effectiveness of the compound of coralline hydroxyapatite porous, platelet-rich plasma and fibrin glue acting as a bone substitute material for repair of bone defects. Methods: 1.5 cm radial segments from the bilateral forearms of New Zealand white rabbits were removed to prepare bone defect models. Then, bone defect models were randomly divided into a compound group (coralline hydroxyapatite porous, platelet-rich plasma and fibrin glue), control group treated with autogenous bone graft, and blank group without implantation. Results and Conclusion: (1) X-ray observation: Bone defects were repaired completely in the compound group till the end of postoperative week 12, appearing with complete plasticity. The healing process in the compound group was synchronized with that in the control group. However, there were no changes in bone defects of the blank group. (2) Histopathological examination: Bone repair basically completed in the compound and control groups at week 12 after operation, presenting with mature lamellar bone and Haversian canals. In the blank group, only a great amount of fibroblasts proliferated, but no bone formed. (3) Biomechanical analysis: The maximum torque and torsional stiffness of the compound group were better than those of the control group at 2 weeks postoperation (P < 0.05). However, there was no significant difference between the maximum torque and torsional stiffness of the compound and control groups. These results demonstrate that the compound of coralline hydroxyapatite porous, platelet-rich plasma and fibrin glue acting as a bone substitute is superior to autologous bone in the early repair of bone defects, which is beneficial to bone healing. Source