Yan J.,Soochow University of China |
Teng F.,Yancheng Institute of Health Science |
Chen W.,Yancheng Third Peoples Hospital |
Ji Y.,Soochow University of China |
Gu Z.,Soochow University of China
Experimental and Therapeutic Medicine | Year: 2012
Hydrogen sulfide (H 2S), is a member of the novel family of endogenous gaseous transmitters, termed gasotransmitters exhibiting diverse physiological activities, and is generated in mammalian tissues mainly by cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3MST) in conjunction with cysteine (aspartate) aminotranferase (CAT). The distributions of these enzymes are species-and tissue-specific. The liver, as the main organ that generates H 2S in vivo, functions in biotransformation and metabolism. However, the liver is vulnerable to damage from internal and external factors, including inflammatory mediators, drugs and poisons. The present study evaluated the endogenous CBS-H 2S synthesis regulating lipopolysaccharide (LPS)-induced apoptosis of hepatic cells. The rat hepatic cell line, BRL, was incubated with LPS for various time periods to establish a cell-damage model. Incubation with LPS resulted in a significant increase in CBS expression and H 2S production. It also stimulated apoptosis and decreased the mitochondrial membrane potential. Pretreatment with the CBS inhibitor aminooxyacetic acid (AOAA) or CBS small interfering RNA (siRNA) decreased LPS-enhanced H 2S production. Notably, apoptosis increased for a short period and then decreased gradually, while the mitochondrial membrane potential demonstrated the opposite trend. These results showed that endogenous CBS-H 2S synthesis demonstrated early anti-apoptotic activity and subsequent pro-apoptotic activity in LPS-induced apoptosis. These results suggest a new approach for developing novel drugs for this condition.
Wang Y.-H.,Yancheng Institute of Health science
Advanced Materials Research | Year: 2014
Image processing has been widely applied in various fields. Photoshop image processing software is the best one for the enthusiasts. At present, more and more people are interested in using Photoshop to create some characteristic pictures, while the key and difficulty part of image processing in Photoshop is matting method(create the image area).In order to help the image processing enthusiasts to accomplish in the process of image processing and create a more beautiful image, this paper introduces several commonly used matting methods in Photoshop and discusses in detail the scope of application, the using methods, the advantages and disadvantages of them. © (2014) Trans Tech Publications, Switzerland.
Yu M.,Yancheng Institute of Health science |
Zhang X.-Y.,Yancheng Institute of Health science |
Yu Q.,Yancheng Institute of Health science
Pakistan Journal of Medical Sciences | Year: 2015
Objective: To investigate the incidence of oral infection with Helicobacter pylori (H. pylori) and identify related epidemiological factors among freshmen of four colleges in Yancheng. Methods: The data, scored positive or negative, were collected on 160 individuals who had been diagnosed by H. pylori Saliva Test Cassette (HPS) during October 2013 to October 2014. H. pylori Saliva Test Cassette (HPS) is to use colloidal gold technique to specifically identify urease in saliva. A standard questionnaire, with variables including sex, educational degree of parents etc., was used in the subjects. Statistical data of diagnostic test were analyzed by SPSS17.0 software. Results: Out of 160, 82 subjects were detected positive and 78 were negative. In univariate analysis, dental plaque, family history of stomach diseases, habit of washing hands before meals and habit of brushing teeth twice daily were associated negatively with H. pylori infection. Multivariate logistic regression analysis showed that dental plaque and family history of stomach diseases were the risk factors which may be associated with H. pylori infection. Conclusions: Dental plaque and family history of gastric diseases were risk factors of oral H. pylori infection. It is vital for the prevention of H. pylori infection to focus on health education and oral hygiene, and avoid transmission by oral-oral route as well. © 2015, Professional Medical Publications, All Rights Reserved.
Cheng J.,Yancheng Institute of Health Science |
Di L.Q.,Nanjing University |
Bi X.L.,Nanjing University
Advanced Materials Research | Year: 2013
Purpose. Our aim was to investigate the uptake kinetics of forsythin in the small intestine and analyze the factors that affect absorption. Methods. A simple and convenient method was established by HPLC to detect forsythin. To characterize forsythin uptake kinetics in different sacs, three different forsythin concentrations, P-glycoprotein inhibitor verapamil hydrochloride and Tween-80 were assayed by the everted rat gut sac model. Results. Forsythin could be absorbed in the whole intestine, the data had concentration and time dependence, and ka had no significant differences (P>0.05) at three concentrations (10, 20, and 40μg*mL-1). The results showed linear correlation between the forsythin absorption in sac contents and the incubation time from 0~90min. Forsythin absorbed dose order in rat small intestine is ileum>jejunum>duodenum. The permeability increased when forsythin was perfused with Tween-80, but P-gp inhibitor verapamil hydrochloride didn't has this effect. Conclusion. The absorption of forsythin in the small intestine is first-order process. The absorption mechanism is inferred the passive diffusion. Forsythin is not the substrate of P-gp. © (2013) Trans Tech Publications, Switzerland.
Song J.,Nanjing Normal University |
Liu H.,Nanjing Normal University |
Ma L.,Nanjing Normal University |
Gao C.,Yancheng Institute of Health science |
And 2 more authors.
Fish and Shellfish Immunology | Year: 2014
Interferon-γ-inducible lysosomal thiol reductase (GILT) plays a key role in the processing and presentation of MHC class II-restricted antigen (Ag) by catalyzing disulfide bond reduction, thus unfolding native protein Ag and facilitating subsequent cleavage by proteases. For this important function in the immune system, we cloned a GILT gene homologue from mandarin fish (designated mGILT), a kind of precious freshwater fish with high market value. Through reverse transcription PCR and rapid amplification of cDNA ends (RACE) strategies, we obtained the full-length cDNA of mGILT, which consists of 1008bp with a 771bp open reading frame, encoding a protein of 256 amino acids, with a putative molecular weight of 28.47kDa. The deduced protein possesses the typical structural features of known GILT proteins, including an active-site motif, a GILT signature sequence, and 6 conserved cysteines. The result of real-time quantitative PCR showed that mGILT mRNA was expressed in a tissue-specific manner. In addition, the expression of mGILT mRNA was obviously up-regulated in splenocytes and kidney after induction with lipopolysaccharide (LPS). Recombinant mGILT fused with His6 tag was efficiently expressed in Escherichia coli BL21 (DE3) and purified using Ni-nitrilotriacetic acid resin. Further study revealed that mGILT exhibit thiol reductase activity on IgG substrate. These results suggest mGILT is highly likely to play a role in the immune responses in mandarin fish. © 2014 Elsevier Ltd.
Wang F.,Yancheng Institute of Health science
Journal of Digital Information Management | Year: 2015
Visualization as one of the important tools for understanding complex phenomenon and resolving large-scale data is observed with a significant application value in natural science field. Since the concept of visualization was proposed in 1986, its research progress is constantly promoted. Based on visualization theory at home and abroad, this study discussed over the significance of introducing visualization theory, performed algorithm design and implementation based on the content and target of computer graphics teaching, and gave out problems found in practical process as well as solutions, in order to change the teaching means of teachers and help students to understand computer graphics algorithm using visualization teaching.
Yang P.,Nanjing Medical University |
Tuo L.,Yancheng Institute of Health Science |
Wu Q.,Yancheng Institute of Health Science |
Cao X.,Nanjing Medical University
Hepato-Gastroenterology | Year: 2014
Background/Aims: Esophageal carcinoma is one of the most aggressive human cancers, and novel treatment modality is required. Although expressing adequate levels of functional tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors DR4/DR5, significant proportion of esophageal cancer cells exhibit resistance to the cytotoxic effect of this ligand. Licochalcone-A (LA), a flavonoid present in a variety of edible plants, exhibits a wide spectrum of pharmacologic properties such as anticancer, antioxidant, and anti-inflammatory activities. Methodology: Eca109 and TE1 cells were cultured and transfected, then their viability was detected using MTT assay. Immunoprecipitation and immunoblotting analysis and RT-PCR analysis were also performed. Results: In this study, we found that LA synergistically caused the TRAIL-induced apoptosis in Eca109 and TE1 cells. Such potentiation was achieved through inhibiting Akt activation and promoting proteasomal degradation of X-linked Inhibitor of Apoptosis Protein (XIAP) which mediated the survival signals and allow the cells to escape from apoptosis in various human cancers. Conclusions: The combination of TRAIL and LA might be a novel therapeutic strategy for esophageal carcinoma patients who fail to respond to standard chemotherapy. © H.G.E. Update Medical Publishing S.A.
Lv G.,Fudan University |
Cui B.,Yancheng Institute of Health science |
Lan H.,Fudan University |
Wen Y.,Fudan University |
And 2 more authors.
Chemical Communications | Year: 2015
Two fluorescent switchable diarylethene derivatives which exhibit high affinity for amyloid-β aggregates with the increase of fluorescence intensity were reported. Moreover, the probes show excellent photochromic and anti-photobleaching properties both in vitro and in vivo. This journal is © The Royal Society of Chemistry 2015.
Wang F.,Yancheng Institute of Health science
BioTechnology: An Indian Journal | Year: 2014
This paper analyzes the concept of queue, the storage structure of the queue data and encoding of the queue data based on queue data. After analyzing and giving an expression to the main structure of queue encoding, this paper discusses DNA encoding process of queue data through instance. Furthermore, an actual queue with details is given out to prove that it is feasible to encode queue structure data by DNA computer. The method and conclusion of this study may shed light upon other studies on the applications of DNA computer. © Trade Science Inc.
PubMed | Yancheng Institute of Health science and China Pharmaceutical University
Type: Journal Article | Journal: PloS one | Year: 2015
Previous studies reported that the expression of miR-23b-27b cluster was downregulated in embryonic brain cortices during hypoxia-induced neuronal apoptosis. However, the mechanism underlying this downregulation is not completely understood. Here, we report that the transcription factor c-Myc plays an important role in regulating the expression of miR-23b-27b cluster during hypoxia. First, the c-Myc protein level was significantly elevated in embryonic brain cortices in a mouse model of fetal distress. Second, forced overexpression or knockdown of c-Myc could suppress or increase the expression of miR-23b-27b cluster polynucleotides. Third, we identified 2 conserved c-Myc binding sites (E-boxes) in the enhancer and promoter regions of miR-23b-27b cluster in the mouse genome. Finally, we showed that elevated c-Myc expression led to an increase in the Apaf-1 level by suppressing miR-23b-27b cluster expression and that this enhanced neuronal sensitivity to apoptosis. In summary, our study demonstrates that c-Myc may suppress the expression of the miR-23b-27b cluster, resulting in additional neuronal apoptosis during hypoxia.