Xia L.-P.,Yancheng Health Vocational and Technical College |
Xia L.-P.,Shanghai University |
Fan F.,Shanghai University |
Tang A.-L.,Shanghai University |
Ye W.-Q.,Shanghai University
International Journal of Clinical and Experimental Medicine | Year: 2014
Neurogenic bladder is a common complication of spinal cord injury and results in urinary bladder dysfunction through lost control of micturition, or urination. Although several treatment options exist, the efficacies of many of these treatments are unknown. In particular, electroacupuncture and bladder training have had some success as individual treatments. The aim of this study was to explore effects of electroacupuncture combined with bladder training on bladder function of patients with neurogenic bladder after spinal cord injury (SCI) above the sacral segment. Forty-two patients with neurogenic bladder after SCI were evenly divided into two groups (n=21) and given only bladder function training (control group) or electroacupuncture combined with bladder function training (treatment group). Urodynamic changes, IPSS score, and therapeutic efficacy were compared between groups pre- and post-treatment. After either treatment, patients had higher bladder volume and bladder compliance, but lower residual urine volume, bladder pressure, rectal pressure, and detrusor pressure, compared to pre-treatment (P<0.05). Compared to controls, treatment group patients had significantly increased bladder volume and bladder compliance, but significantly decreased residual urine volume, bladder pressure, rectal pressure, and detrusor pressure (P<0.05). Treatment group patients had lower IPSS scores post-treatment (P<0.05) and better therapeutic efficacy (P<0.05) than control group patients. Altogether, our results suggest that electroacupuncture combined with bladder function training can clinically improve bladder function of patients with neurogenic bladder after SCI above the sacral segment.
Xia L.P.,Yancheng Health Vocational and Technical College
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2010
To study the role of autophagy in the death of dopaminergic neurons induced by 6-hydroxydopamine (6-OHDA). Rat models of Parkinson disease (PD) were established by stereotaxic administration of 6-OHDA (8 μg) into the unilateral substantia nigra par compact (SNpc). Autophagosomes in the SNpc were observed with transmission electron microscopy (TEM), and the expression of autophagy-related protein LC3 was determined with immunofluorescence (IF) assay. Under TEM, the autophagosomes were found in the ipsilateral SNpc 6-24 h after 6-OHDA injection, which suggested the activation of autophagy. IF assay showed significantly increased LC3 expression in 6-OHDA-damaged TH-positive neurons as compared to the control group. The increase of autophagosomes and activation of autophagy may play a role in dopaminergic neuron death induced by 6-OHDA.
Cui Y.,Yancheng Health Vocational and Technical College |
Cui Y.,University of Texas at San Antonio |
Bastien D.A.,University of Texas at San Antonio
International Journal of Biological Sciences | Year: 2012
In the present study, an equilibrated system for the Aqy1 tetramer was developed, and molecular biophysics modeling showed that the Aqy1 channel was blocked by Tyr-31 in the N-terminus, which was also supported by the free energy profiles. However, bioinformatics analysis of the amino acid sequence of Aqy1 indicated this Tyr-31 is not conserved across all fungi. Analysis of the equilibrated structure showed that the central pore along the four-fold axis of the tetramers is formed with hydrophobic amino acid residues. In particular, Phe-90, Trp-198, and Phe-202 form the narrowest part of the pore. Therefore, water molecules are not expected to translocate through the central pore, a hypothesis that we confirmed by molecular dynamics simulations. Each monomer of the Aqy1 tetramers forms a channel whose walls consist mostly of hydrophilic residues, transporting through the selectivity filter containing Arg-227, His-212, Phe-92, and Ala-221, and the two conserved Asn-Pro-Ala (NPA) motifs containing asparagines 224 and 112. In summary, not all fungal aquaporins share the same gating mechanism by a tyrosine residue in the N-terminus, and the structural analysis in the present study should aid our understanding of aquaporin structure and its functional implications. © Ivyspring International Publisher.
Cui Y.,Yancheng Health Vocational and Technical College
Parasites and Vectors | Year: 2014
Domestic mite species found in indoor environments and in warm or tropical regions are well known for causing allergic disorders. However, little is known about human acariasis, in which mites invade and parasitize the human body in various tissues from the gastrointestinal tract to the lung. Here, we summarize the reported cases of human acariasis of pulmonary, intestinal, oral (anaphylaxis), urinary, otic, and vaginal systems. Because the clinical symptoms of acariasis often overlap with other disease symptoms leading to frequent misdiagnosis, we highlight the need for more attention on these infections. © 2014 CUI; licensee BioMed Central Ltd.
Bian L.-Y.,Yancheng Health Vocational and Technical College
Chinese Journal of New Drugs | Year: 2016
Objective:To prepare naringenin-loaded solid lipid nanoparticles (NRG-SLNs) lyophilized powder, and investigate its physicochemical properties, release characteristics, and the pharmacokinetic characteristics in rats after pulmonary delivery. Methods:NRG-SLNs were prepared by solvent emulsification-evaporation method, the the formulation was optimized by orthogonal design with encapsulation efficiency as indicator. The particle size, morphology, Zeta potential, polydispersity index(PDI), and in vitro drug release behavior were determined. The appearance and color of lyophilized powder were evaluated with FT-IR analysis. The pulmonary pharmacokinetics of NRG-SLNs in rats was studied after pulmonary instillation. Results:The NRG-SLNs showed spherical shape with an even distribution of diameter. The particle size was (134.81±14) nm, PDI was 0.238, Zeta potential was (-27.6±0.87) mV, entrapment efficiency was (81.2±1.6)%, and drug loading was (8.11±0.5)%(n=3). 5% mannitol was the best protective agent for the preparation of NRG-SLNs lyophilized powder. It was indicated that the drug was dispersed in amorphous state in SLNs. The dissolution experiments showed that NRG-SLN had obviously sustained release compared with the bulk drug. After pulmonary administration to rats, the pharmacokinetic parameters of naringenin liposomes and solution were as follows:Cmax(173.00±25.05) and (280.00±36.34) ng·mL-1, AUC0-t(939.32±190.18) and (3 440.23±533.88) ng·mL-1·h, MRT (7.29±0.44)and (24.29±9.27) h, respectively. Conclusion:Naringenin solid lipid nanoparticles were successfully developed in this study. The in vitro drug release experiments showed that the preparation has obvious sustained-release effect, which overcame the bad solubility and stability of naringenin and improved the in vivo bioavailability, thus providing a suitable new dosage forms for pulmonary drug delivery. Therefore, naringenin solid lipid nanoparticles is a new dosage form with great research potential. Copyright © 2016 by the Editorial Board of Chinese Journal of Contemporary Neurology and Neurosurgery
Zhou Y.,Yancheng Health Vocational and Technical College |
Jiang Y.-Q.,The First Peoples Hospital of Yancheng City |
Wang W.-X.,The First Peoples Hospital of Yancheng City |
Zhou Z.-X.,The First Peoples Hospital of Yancheng City |
And 3 more authors.
Human Immunology | Year: 2012
Previous work indicated that high mobility group box-1 (HMGB1) protein may be involved in neutrophilic asthma. Here, we sought to investigate the correlation between HMGB1 and one of its receptors, receptor for advanced glycosylation end products (RAGE), with the severity of bronchial asthma. Compared to the control group (30 healthy individuals), patients in the asthma group (n=72) exhibited a higher percentage of neutrophils and higher HMGB1 and RAGE levels in induced sputum samples (P<0.05). Concurrently, FEV1% was significantly lower in the asthma group (P<0.05). Further, compared to mild and moderate asthma, in patients with severe asthma ACQ scores, the percentage of neutrophils, and HMGB1 levels were significantly higher, while FEV1% was significantly lower (P<0.05). The percentage of neutrophils and HMGB1 and RAGE levels were lower after treatment than before treatment (P<0.05). Finally, negative correlations were observed between HMGB1 or RAGE levels and FEV1% (r=-0.777 and r=-0.291, P<0.05), and positive correlations were detected between HMGB1 or RAGE levels and percentage of neutrophils (r=0.803 and r=0.326, P<0.05). Additionally, positive correlations were observed between HMGB1 and RAGE levels within the asthma group (r=0.306, P<0.05). Therefore, HMGB1 protein levels correlate with the severity of asthma, and HMGB1 may contribute to the inflammatory process of asthma. © 2012 .
Cui Y.,Yancheng Health Vocational and Technical College
Molecular Biology Reports | Year: 2013
Mite allergens contribute to a significant proportion of human allergic symptoms, including asthma and rhinitis. The development of therapies to treat and prevent these symptoms depends largely on our understanding of the properties of these allergens. Much effort has been devoted to determining the structure and organization of mite allergens, particularly of the house dust mites, toward understanding their activities and how they elicit immunological responses in humans. Here, we review the structural biology of the major allergens from two species of house dust mites, Dermatophagoides farinae and D. pteronyssinus, as well as allergens from a storage mite, Blomia tropicalis. The knowledge gained from the structural biology of these allergens will enable progress in producing novel, more effective treatments for mite allergies based on specific immunotherapy approaches. © 2012 Springer Science+Business Media Dordrecht.
Li L.,China Pharmaceutical University |
Lin R.,Yancheng Health Vocational and Technical College |
He H.,China Pharmaceutical University |
Jiang L.,China Pharmaceutical University |
Gao M.,China Pharmaceutical University
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2013
Carboxylated single-walled carbon nanotubes (c-SWNTs) were synthesized prosperously in order to improve dispersion of raw carbon nanotubes. Then, bovine serum albumin (BSA) was used as the template protein to study the biocompatibility of c-SWNTs by UV-Vis, fluorescence and circular dichroism (CD) spectroscopic methods at the molecular level. Results from fluorescence spectrum showed obvious decreases in fluorescence intensity of BSA induced by c-SWNTs, indicating the occurrence of interaction between BSA and c-SWNTs. Static quenching effect of c-SWNTs was verified by linear Stern-Volmer plots and K SV values. Thermodynamic parameters at different temperatures demonstrated that the interaction between c-SWNTs and BSA was mainly favored by hydrophobic force. In addition, Na+ interfered with the quenching effect of c-SWNTs, which revealed that electrostatic force played a role in binding roles of BSA to c-SWNTs simultaneously. The results of UV and synchronous fluorescence spectrum validated that hydrophobicity of amino acid residues expressly increased with the addition of c-SWNTs. The content of α-helix structure in BSA decreased by 14.06% with c-SWNTs viewed from CD spectrum. Effect of SWNTs on the conformation of BSA could be controlled by the surface chemistry of SWNTs. © 2012 Elsevier B.V. All rights reserved.
Cui Y.,Yancheng Health Vocational and Technical College
Clinical Reviews in Allergy and Immunology | Year: 2014
House dust mites and storage mites produce a number of allergens that can induce hypersensitivity reactions in humans and result in allergic diseases like asthma, rhinitis, and dermatitis. Recent advances in identifying and characterizing these allergens—and, in particular, their immunoglobulin E (IgE)-binding epitopes—have produced a wealth of knowledge. Here, methods for identifying IgE-binding epitopes, from immunoassays to in silico approaches, are summarized and placed in context with the identification of epitopes of mite allergens, particularly from the Dermatophagoides spp. major allergen groups 1 and 2. Finally, the transfer of this information to the clinical development and application of new diagnostic and immunotherapeutic approaches is discussed. While progress in recent years has built on the specific immunotherapies established decades ago, much work remains to be done to mitigate mite allergic disease. Future studies should seek to identify epitopes for mite species beyond Dermatophagoides and for minor allergens. Efforts in translational medicine should use the current epitope data to develop modified allergens for immunotherapy. © 2013, Springer Science+Business Media New York.
Wang N.,Yancheng Health Vocational and Technical College |
Ding L.,East China Normal University
Journal of Polymer Research | Year: 2012
A novel cyclodextrin-based hyperbranched polymer (HBP) was synthetized via acyclic diene metathesis (ADMET) polymerization in homogeneous water/organic mixtures. A modified α-cyclodextrin (α-CD) molecule with one electron-rich terminal alkene and many electron-poor acrylates was first prepared through the esterification reaction, and then utilized as an AB n-type monomer for subsequent ADMET polymerization between alkene and acrylate using the second generation Hoveyda-Grubbs catalyst, yielding HBP with the reaction time prolonged. The chemical structures of monomer and HBP were characterized by elemental analysis, IR, gel permeation chromatography with multiangle laser light scattering, and NMR measurements. The degree of branching was determined by using 1H NMR spectroscopy and the values ranged from 0.51 to 0.42. Influence of the molecular weight on the properties (thermal stability and solubility) was also investigated. The resulting HBPs showed the good thermal stability, and higher molecular weight resulted in higher decomposition temperature from 361 °C to 383 °C. These thermally stable HBPs also displayed the excellent solubility in aprotic polar solvents. © Springer Science+Business Media B.V. 2012.