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Wang Z.H.,Shanghai JiaoTong University | Wang Z.H.,Yancheng Peoples First Hospital | Zhu W.,Shanghai JiaoTong University | Tao J.P.,Shanghai JiaoTong University | And 2 more authors.
Brazilian Journal of Medical and Biological Research | Year: 2013

Angiopoietin (Ang)-1 and Ang-2 interact in angiogenesis to activate the Tie-2 receptor, which may be involved in new vessel maturation and regression. Mast cells (MCs) are also involved in formation of new blood vessels and angiogenesis. The present study was designed to test whether MCs can mediate angiogenesis in myocardial microvascular endothelial cells (MMVECs). Using a rat MMVEC and MC co-culture system, we observed that Ang-1 protein levels were very low even though its mRNA levels were increased by MCs. Interestingly, MCs were able to enhance migration, proliferation, and capillary-like tube formation, which were associated with suppressed Ang-2 protein expression, but not Tie-2 expression levels. These MCs induced effects that could be reversed by either tryptase inhibitor [N-tosyl-L-lysine chloromethyl ketone (TLCK)] or chymase inhibitor (N-tosyl-L-phenylalanyl chloromethyl ketone), with TLCK showing greater effects. In conclusion, our data indicated that MCs can interrupt neovessel maturation via suppression of the Ang-2/Tie-2 signaling pathway.

Wang Y.,Zhejiang Chinese Medical University | Wu J.,Yancheng Peoples First Hospital | Cao Y.,Zhejiang Chinese Medical University
International Journal of Clinical and Experimental Medicine | Year: 2015

Aim: to analyze the detection rate of intestinal enteroaggregative Escherichia coli (EAEC) in healthy elderly (≥60 years) individuals in the Hangzhou area of China, and to investigate the extended spectrum β-lactamases and virulence genes of EAEC. Methods: Stool specimens provided by healthy elderly individuals were cultured on blood agar, SS, and MAC plates. The bacterial strains were identified using Vitek-2 Compact automatic microorganism identification system and mass spectrometry. The resistance phenotypes of the bacteria were determined using the double-disk synergy method. The resistance genes and the EAEC virulence gene, astA and aggR, were amplified by PCR and compared to the sequences available in Gen Bank. Results: Among the 1050 healthy volunteers, the majority of bacteria were E. coli, accounting for 960 strains, with an ESBL-positive rate of 36.3% (348/960). The EAEC detection rate was 10% (96/960), among them, 84 strains were astA, the detection rate of which was 8.75%, 12 strains were aggR, the detection rate of which was 1.25%. The ESBL-positive rate of EAEC strains were 56.25% (54/96), all of which carried the CTX-M type, with the CTX-M-14 predominating at 66.7% (36/54). Conclusions: The ESBL-positive rate of intestinal E. coli in healthy elderly individuals in the Hangzhou area of China was higher than the rate detected in other regions of china, and there was a high rate of antibiotic resistance among the intestinal EAEC in healthy elderly individuals. The results of this study suggest that EAEC is not only a pathogenic bacteria detected in diarrhea patients, but can also be present in healthy individuals, and high-resistance clinical strains have spread to the healthy population in the Hangzhou area. So vigilance is critical. © 2015 E-Century Publishing Corporation. All rights reserved.

Wu J.,Yancheng Peoples First Hospital | Shang A.-Q.,The Sixth Peoples Hospital Of Yancheng | Lu W.-Y.,The Sixth Peoples Hospital Of Yancheng
International Journal of Clinical and Experimental Medicine | Year: 2016

Objective: To investigate the significance of neutrophil gelatinase-associated lipocalin (NGAL) and matrix metalloproteinase-9 (MMP-9) protein expression in epithelial ovarian cancers. Methods: NGAL and MMP-9 serum and ovarian tissue levels were detected in 150 ovarian epithelial cancers. 42 benign ovarian tumors and 30 healthy women as controls by immunohistochemistry technique and reverse transcriptase-polymerase chain reaction (RT-PCR). Results: NGAL and MMP-9 expression in tissue and serum in ovarian cancer group were significantly higher than that in benign ovarian tumor group and normal control group (both P<0.05). Two high expression and clinical staging, lymph node metastasis positive correlation (both P<0.05). Moreover, in the serum and ovarian tissue of patients with epithelial ovarian cancer, the expression of NGAL had positive correlation with histological differentiation (both P<0.05) and the expression level of MMP-9 had negative correlation with histological differentiation (P<0.05). In patients with ovarian carcinoma tissue or serum, NGAL expression were positive expression of MMP-9 (r=0.740, r=0.676, both P<0.05). Conclusions: The high expression of NGAL and MMP-9 in epithelial ovarian cancer may be associated with the initiation and progression of epithelial ovarian cancer. © 2016, E-Century Publishing Corporation. All rights reserved.

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