No 1 Peoples Hospital of Yancheng City

Yancheng, China

No 1 Peoples Hospital of Yancheng City

Yancheng, China
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Tang D.,Nanjing Medical University | Li J.-P.,No 1 Peoples Hospital Of Yancheng City | Zheng X.-F.,Nanjing Medical University | He X.-P.,Nanjing Medical University | And 4 more authors.
Chinese Pharmacological Bulletin | Year: 2012

Aim: To investigate the effects of ursolic acid on cell proliferation and apoptosis in cyclooxygenase-2 (COX-2) -expressing (SGC-7901) and -non-expressing (MGC-803) gastric cancer cell lines and to reveal whether the signal transducer and activator of transcription 3 (STAT3) signaling pathway mediates these effects. Methods: SGC-7901 and MGC-803 cells were seeded in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and routinely incubated for 24 h. After treatment with ursolic acid at a final concentration of 10, 20, 40 and 80 μmol·L -1 for 24, 36 and 48 h, the cell proliferation was determined with MTT colorimetric assay. Hoechst staining was used to observe the morphologic changes of apoptotic cells. Apoptosis rates were detected by flow cytometric analysis. The expression of STAT3, phosphorylated STAT3 (p-STAT3), COX-2 and procaspase-3 was detected by Western blot analysis. Results: Ursolic acid significantly inhibited SGC-7901 and MGC-803 cell proliferation in a dose- and time-dependent manner. Hoechst staining showed typical apoptotic morphologic changes in both SGC-7901 and MGC-803 cells treated with ursolic acid. The apoptosis rates in SGC-7901 and MGC-803 cells treated with 10-80 μmol·L -1 ursolic acid for 24 h were (4.7 ± 0.4)% and (3.3 ± 0.2)%, (6.4 ± 0.5)% and (4.6 ± 0.3)%, (9.9 ± 1.8)% and (6.8 ± 0.5)%, (14.5 ± 1.7)% and (10.3 ± 1.6)%, respectively. Ursolic acid dose-dependently inhibited STAT3 phosphorylation in both SGC-7901 and MGC-803 cells as well as COX-2 expression in SGC-7901 cells and procaspase-3 expression in MGC-803 cells. Conclusions: Ursolic acid inhibits proliferation and induces apoptosis of gastric cancer cells, which may be mediated by down-regulation of COX-2 or procaspase-3 expression through inhibition of the STAT3 signaling pathway.


Cao L.-H.,No 1 Peoples Hospital of Yancheng City | Zhang M.-H.,No 1 Peoples Hospital of Yancheng City | Lu D.,No 1 Peoples Hospital of Yancheng City | Qin P.-P.,No 1 Peoples Hospital of Yancheng City | Zhou Y.,No 1 Peoples Hospital of Yancheng City
Journal of Clinical Dermatology | Year: 2012

Objectives: To observe the clinical effects of long-pulsed 1 064 nm Nd:YAG laser in the treatment of hemangiomas of skin in infant and investigate its related factors. Methods: Total 215 cases were treated with long-pulsed 1 064 nm Nd:YAG laser according to different disease types, including 19 cases of port wine stains, 179 cases of strawberry hemangiomas and 17 cases of mixed hemangiomas. The association between clinical effect and types, size, location and times of treatment of hemangiomas was also analyzed. Results: Among 215 cases, 133 were cured, 67 gained marked effect, 8 gaining effective therapy and 7 ineffective. The cure rate and total efficacy rate were 61.9% and 93.1%, respectively. The effect was related with the type, size and treatment times of hemangiomas. However, the location of hemangiomas was not associated with the therapy effect. The side-effect rate was 48.8% with temporary hyper-pigmentation, 8.3% with temporary hypo-pigmentation, 4.6% with superficial scar and 3.7% with hair reduction or disappearance. Conclusion: The long-pulsed 1 064nm Nd:YAG laser is an effective, safe and low side-effect treatment for infancy hemangiomas.

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