Zhang C.,Yanan University
Acta biochimica et biophysica Sinica | Year: 2013
MicroRNAs (miRNAs) function as negative regulators of gene expression involved in cancer metastasis. The aim of this study is to investigate the potential roles of miR-218 in non-small cell lung cancer and validate its regulation mechanism. Functional studies showed that miR-218 overexpression inhibited cell migration and invasion, but had no effect on cell viability. Enhanced green fluorescent protein reporter assay, real-time polymerase chain reaction and western blot analysis confirmed that miR-218 suppressed the expression of high mobility group box-1 (HMGB1) by directly targeting its 3'-untranslated region. Accordingly, silencing of HMGB1 accorded with the effects of miR-218 on cell migration and invasion, and overexpression of HMGB1 can restore cell migration and invasion which were reduced by miR-218. In conclusion, these findings demonstrate that miR-218 functions as a tumor suppressor in lung cancer. Furthermore, miR-218 may act as a potential therapeutic biomarker for metastatic lung cancer patients.
Shen W.-W.,Shanghai JiaoTong University |
Zeng Z.,Shanghai JiaoTong University |
Zhu W.-X.,Yanan University |
Fu G.-H.,Shanghai JiaoTong University
Journal of Molecular Medicine | Year: 2013
Studies have shown that the expression of CD133, leucine-rich-repeat- containing G-protein-coupled receptor 5 (Lgr5), and ATP binding cassette (ABC)G2 proteins is associated with malignancy and poor prognosis in colon cancer. However, molecular regulation mechanism of the three proteins has not been elucidated. Here, we report that microRNA-142-3p (miR-142-3p) inhibits the expression of CD133, Lgr5, and ABCG2 in colon cancer cells by binding to both the 3′-untranslated region and the coding sequences of the three genes. The miR-142-3p was markedly decreased in colon cancer specimens, in which it was negatively correlated with the expression of CD133, Lgr5, and ABCG2. Reduction of miR-142-3p corresponds to poor differentiation and bigger tumor size in colon cancers. Moreover, miR-142-3p levels were reduced in cells that formed spheres compared to cells that were cultured in regular media. Transfection of miR-142-3p mimics in colon cancer cells downregulated cyclin D1 expression, induced G1 phase cell cycle arrest, and elevated the sensitivity of the cells to 5-fluorouracil. Furthermore, OCT4 suppressed miR-142-3p, and hypomethylation of the OCT4 promoter was associated with a reduction in miR-142-3p. Finally, the miR-142-3p inhibited the growth of colon cancer cells in vivo, which was accompanied by the downregulation of CD133, Lgr5, and ABCG2 in tumor tissues. Our results elucidate a novel regulation pathway in colon cancer cells and suggest a potential therapeutic approach for colon cancer therapy. © 2013 Springer-Verlag Berlin Heidelberg.
He G.,Yanan University |
Yan H.,Shanghai Yaming Lighting Co.
Optics Express | Year: 2011
A model for spectra of the phosphor-coated white LED (p-W LED) with a blue chip, a red chip, and green and yellow phosphors is presented. The optimal spectra of p-W LEDs with correlated color temperatures (CCTs) of 2700-6500 K have been obtained with a nonlinear program for maximizing luminous efficacy of radiation (LER) under conditions of both color-rendering indices (CRIs) and special CRIs of R9 strong red above 98. The simulation results show that p-W LEDs with one InGaN blue (450 nm) chip, one AlGaInP red (634 nm) chip, and green (507 nm) and yellow (580 nm) silicate phosphors can realize white lights with CRIs of about 98 and special CRIs of R9 for strong red above 98. The average of the special CRIs R9 to R12 for the four saturated colors (red, yellow, green, and blue) is above 95. R13 for the skin of women's faces at about 100, as well as LERs above 296 lm/W at CCTs of 2700-6500 K. LERs of excellent CRI p-W LEDs with one InGaN blue chip, one AlGaInP red chip, and green and yellow silicate phosphors increased by 19-49% when compared with that of excellent CRI p-W LEDs with one InGaN blue chip and green and yellow silicate phosphors, as well as red nitride phosphor. © 2011 Optical Society of America.
He G.,Yanan University
Optics Letters | Year: 2011
This is a comment on a previous Letter [Opt. Lett. 35, 3372 (2010). The chromaticity coordinates of nanocrystal quantum dot (NQD)-integrated WLED1, WLED2, and WLED3 are out of the range of the chromaticity tolerance quadrangles of white light sources. So these NQD WLEDs do not satisfy the requirements recommended for general lighting with solid-state lighting products to ensure high-quality white light. Furthermore, correlated color temperatures of WLED2 and WLED3 should be 2682 and 2527K, respectively, according to the chromaticity coordinates of WLED2 and WLED3 at 12mA, not 2781 and 2390K. The NQD-integrated WLED1 and WLED2 located in tolerance quadrangles are simulated by the changing spectral power of green-, yellow-, and orange-emitting NQDs. The simulation results are presented. © 2011 Optical Society of America.
Wang J.,Yanan University
International Journal of Digital Content Technology and its Applications | Year: 2012
The problem of high-new technology innovation project performance evaluation with uncertain linguistic variables is the multiple attribute decision making (MADM) problems. In this paper, we investigate the multiple attribute decision making (MADM) problems for high-new technology innovation project performance evaluation with uncertain linguistic variables. We utilize the uncertain linguistic weighted average (ULWA) operator to aggregate the uncertain linguistic variables corresponding to each alternative and get the overall value of the alternatives, then rank the alternatives and select the most desirable one(s). Finally, an illustrative example is given.