Yanan Hospital Of Kunming

Kunming, China

Yanan Hospital Of Kunming

Kunming, China
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Li Y.,Kunming Medical University | Yao Y.,Peking Union Medical College | Qian X.,Yanan Hospital of Kunming | Shi L.,Peking Union Medical College | And 2 more authors.
PLoS ONE | Year: 2015

Recently, in vitro studies have demonstrated that adiponectin has antiangiogenic and tumor growth-limiting properties. Additionally, serum adiponectin levels have been associated with the risk of several cancers; specifically, serum adiponectin was significantly lower in lung cancer patients with advanced-stage disease. In this study, we examined the association of adiponectin gene promoter variations associated with adiponectin gene expression and plasma levels in non-small cell lung cancer (NSCLC) in a Han Chinese population. A total of 319 patients with NSCLC and 489 healthy individuals were recruited to evaluate the association of four adiponectin gene promoter single-nucleotide polymorphisms (SNPs) (SNP-12140G>A, SNP-11426A>G, SNP-11391G>A and SNP-11377C>G) with NSCLS risk. Additionally, we constructed haplotypes of these four SNPs and evaluated the association of these haplotypes with NSCLS risk. Our results showed that among these four SNPs, only SNP-12140G>A was associated with NSCLC risk(P<0.05). The haplotype analysis showed that no haplotype was associated with NSCLC after performing a Bonferroni correction (P>0.05). Additionally, an association analysis of the four SNPs stratified into pathologic stages I+II and III+IV showed that these SNPs did not exhibit significant differences between pathologic stages I+II and III+IV. Moreover, we did not observe any differences in allele and genotype frequency for these SNPs between adenocarcinoma and squamous cell carcinoma. Our results indicated that the G allele of SNP-12140may be a risk factor for NSCLC (OR = 1.516; 95% CI: 1.098-2.094) in this Han Chinese population. © 2015 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


PubMed | Yan`an Hospital of Kunming, Kunming Medical University and Peking Union Medical College
Type: Journal Article | Journal: PloS one | Year: 2015

Recently, in vitro studies have demonstrated that adiponectin has antiangiogenic and tumor growth-limiting properties. Additionally, serum adiponectin levels have been associated with the risk of several cancers; specifically, serum adiponectin was significantly lower in lung cancer patients with advanced-stage disease. In this study, we examined the association of adiponectin gene promoter variations associated with adiponectin gene expression and plasma levels in non-small cell lung cancer (NSCLC) in a Han Chinese population. A total of 319 patients with NSCLC and 489 healthy individuals were recruited to evaluate the association of four adiponectin gene promoter single-nucleotide polymorphisms (SNPs) (SNP-12140G>A, SNP-11426A>G, SNP-11391G>A and SNP-11377C>G) with NSCLS risk. Additionally, we constructed haplotypes of these four SNPs and evaluated the association of these haplotypes with NSCLS risk. Our results showed that among these four SNPs, only SNP-12140G>A was associated with NSCLC risk (P<0.05). The haplotype analysis showed that no haplotype was associated with NSCLC after performing a Bonferroni correction (P>0.05). Additionally, an association analysis of the four SNPs stratified into pathologic stages I+II and III+IV showed that these SNPs did not exhibit significant differences between pathologic stages I+II and III+IV. Moreover, we did not observe any differences in allele and genotype frequency for these SNPs between adenocarcinoma and squamous cell carcinoma. Our results indicated that the G allele of SNP-12140 may be a risk factor for NSCLC (OR = 1.516; 95% CI: 1.098-2.094) in this Han Chinese population.


PubMed | Yanan Hospital of Kunming, Chinese Academy of Sciences, Wenzhou University, General Hospital of Yunnan Armed Police Force and Kunming Medical University
Type: | Journal: Scientific reports | Year: 2016

Human papillomavirus (HPV) infection plays a key role in the development of cervical cancer. The aim of the current study was to investigate the HPV type distribution in Chinese women from Yunnan Province, southwest China. A total of 28,457 individuals ranging in age from 17-84 years were recruited from 13 clinical hospitals located in 10 different regions of Yunnan Province. Cervicovaginal swabs were collected from each participant, and HPV screening was performed using Luminex xMAP technology. Our results showed that the HPV prevalence was 12.9% in Yunnan Province. Overall, 10.6% of the individuals were positive for a single HPV type, and 2.3% were positive for multiple types. Among the individuals who tested positive for a single HPV type and multiple HPV types, the three most prevalent high-risk types were 52, 16, and 58. Age subgroup analysis showed two peaks for the frequencies of single and multiple HPV infections, one for the group of women under 25 years old, and the other for the group over 56 years old. Here, we present data regarding the prevalence and type distribution of HPV infection, which will aid in the estimation of the potential clinical benefit and cost-effectiveness of HPV screening and vaccination in China.


Zhou S.,Central South University | Zhou S.,Hunan University | Wang B.,Central South University | Hu J.,Central South University | And 7 more authors.
Tumor Biology | Year: 2016

MicroRNAs (miRNAs) are a new class of prognostic and diagnostic biomarkers for many cancers. Recent studies have shown that miRNAs are highly stable in plasma/serum. The aim of this study was to investigate the role of miR-421 in osteosarcoma. We found that the serum expression levels of miR-421 were significantly higher in osteosarcoma patients than those in healthy volunteers. Moreover, miR-421 expression was significantly higher in osteosarcoma tissues compared with that in the adjacent normal tissues. Meanwhile, the expression of miR-421 was upregulated in 90 % (36/40) osteosarcoma tissues compared to non-tumor tissues. More importantly, the expression levels of miR-421 in osteosarcoma tissues were correlated with those in patients’ serum. In addition, patients with high miR-421 expression had shorter overall survival (OS) than those with low expressions. We also found that overexpression of miR-421 promoted osteosarcoma cell line MG-63 proliferation, migration, and invasion. In conclusion, miR-421 expression levels were upregulated in osteosarcoma tissue and serum and it may be a useful marker for diagnosis of osteosarcoma. © 2016 International Society of Oncology and BioMarkers (ISOBM)


Hu J.,The First Peoples Hospital Of Kunming | Shu J.,Kunming Medical University | Yuan X.,The First Peoples Hospital Of Kunming | Nie B.,The First Peoples Hospital Of Kunming | And 4 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Objective: To explore transplanting autologous bone marrow mesenchymal stem cells (MSCs) compounded with “two-phase” autologous bone matrix gelatin (BMG) into the defects after surgical removal of rabbit intervertebral disc and the feasibility and efficacy of repairing disc. Methods: 48 experimental Japanese rabbits were randomly divided into control group (n=24) and experimental group (n=24). After rabbit models of total removing intervertebral disc were made, experimental group was implanted with autogeneic cell-carrier complex, while control group was only implanted with autologous BMG. Then these rabbits were sacrificed at the 8th and 12th week. Specimens were obtained and underwent general observation and HE staining. Sulfuric acid-carbazole method was used to assay aggrecan, while immunohistochemistry and RT-PCR were performed to detect Collagen II. Results: Intervertebral discs of rabbits in experimental group were repaired with the growth of cartilage endplate and fibrocartilage tissue, while repairing discs in control group was not as good as that in experimental group. Moreover, levels of aggrecan and Collagen II of cartilage endplate and fibrocartilage tissue in experimental group were significantly increased when compared with that in control group (P<0.05) and the difference was statistically significant. Conclusion: MSCs can repair the rabbit inverterbral disc with the growth of cartilage endplate and fibrocartilage tissue. © 2016, E-Century Publishing Corporation. All rights reserved.


PubMed | Hunan University, Yanan Hospital of Kunming and Central South University
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2016

MicroRNAs (miRNAs) are a new class of prognostic and diagnostic biomarkers for many cancers. Recent studies have shown that miRNAs are highly stable in plasma/serum. The aim of this study was to investigate the role of miR-421 in osteosarcoma. We found that the serum expression levels of miR-421 were significantly higher in osteosarcoma patients than those in healthy volunteers. Moreover, miR-421 expression was significantly higher in osteosarcoma tissues compared with that in the adjacent normal tissues. Meanwhile, the expression of miR-421 was upregulated in 90% (36/40) osteosarcoma tissues compared to non-tumor tissues. More importantly, the expression levels of miR-421 in osteosarcoma tissues were correlated with those in patients serum. In addition, patients with high miR-421 expression had shorter overall survival (OS) than those with low expressions. We also found that overexpression of miR-421 promoted osteosarcoma cell line MG-63 proliferation, migration, and invasion. In conclusion, miR-421 expression levels were upregulated in osteosarcoma tissue and serum and it may be a useful marker for diagnosis of osteosarcoma.


Zhang L.,The First Peoples Hospital Of Qujing City | Yang J.,The First Peoples Hospital Of Qujing City | Xue Q.,Yanan Hospital of Kunming | Yang D.,Yanan Hospital of Kunming | And 6 more authors.
Journal of Thrombosis and Thrombolysis | Year: 2016

The expression of let-7 family members was differentiated in ischemic stroke (IS), functioning as an important regulating molecular in the pathophysiology of stroke. We hypothesized that genetic polymorphism in the promoters of let-7 family may be associated with the risk of IS. To test this hypothesis, we investigated the association of the rs10877887 and rs13293512 in the promoters of let-7 family with the susceptibility to IS. A hospital-based case–control study was performed. The rs10877887 genotype was determined by using a polymerase chain reaction-restriction fragment length polymorphism assay, and the rs13293512 genotype was determined by using a TaqMan assay. We found that the rs13293512CC genotype was associated with a reduced risk of IS (CC vs. TT: adjusted OR = 0.43, 95 % CI 0.26–0.71; dominant model: adjusted OR = 0.70, 95 % CI 0.49–0.98; recessive model: adjusted OR = 0.45, 95 % CI, 0.28-0.73). Stratification analysis showed that the rs10877887TT carriers had a higher level of total cholesterol compared to rs10877887TC/CC carriers (P = 0.03). Combined analysis showed that the rs10877887TC/CC and rs13293512TC/CC genotypes had a reduced risk of IS risk (adjusted OR = 0.58, 95 % CI 0.36–0.95). Our findings suggest that the rs13293512 polymorphism may be a protective factor for the development of IS. © 2016 Springer Science+Business Media New York


Lei P.,Peoples Hospital Of Songgang | Lei G.,Guangdong Medical College | Tian J.,Kunming Medical University | Tian J.,Yanan Hospital Of Kunming | And 3 more authors.
International Journal of Colorectal Disease | Year: 2014

Purpose: This paper is aimed to develop the irritable bowel syndrome (IBS) scale of the system of Quality of Life Instruments for Chronic Diseases (QLICD-IBS) by the modular approach and validate it by both classical test theory and generalizability theory. Methods: The QLICD-IBS was developed based on programmed decision procedures with multiple nominal and focus group discussions, in-depth interview, and quantitative statistical procedures. One hundred twelve inpatients with IBS were used to provide the data measuring QOL three times before and after treatments. The psychometric properties of the scale were evaluated with respect to validity, reliability, and responsiveness employing correlation analysis, factor analyses, multi-trait scaling analysis, t tests and also G studies and D studies of generalizability theory analysis. Results: Multi-trait scaling analysis, correlation, and factor analyses confirmed good construct validity and criterion-related validity when using SF-36 as a criterion. Test-retest reliability coefficients (Pearson r and intra-class correlation (ICC)) for the overall score and all domains were higher than 0.80; the internal consistency α for all domains at two measurements were higher than 0.70 except for the social domain (0.55 and 0.67, respectively). The overall score and scores for all domains/facets had statistically significant changes after treatments with moderate or higher effect size standardized response mean (SRM) ranging from 0.72 to 1.02 at domain levels. G coefficients and index of dependability (Ф coefficients) confirmed the reliability of the scale further with more exact variance components. Conclusions: The QLICD-IBS has good validity, reliability, responsiveness, and some highlights and can be used as the quality of life instrument for patients with IBS. © 2014, Springer-Verlag Berlin Heidelberg.


Shuai L.,Yanan Hospital of Kunming | Wang J.,Kunming Forensic Hospital | Jing Q.,Kunming Medical University | Xu B.,Kunming Forensic Hospital | And 3 more authors.
Journal of Forensic Medicine | Year: 2014

Objective: To observe and analyze the mutation phenomenon of 17 STR loci of PowerPlex® 18D Kit in paternity test of Yunnan population. Methods: The DNA was extracted by Chelex-100 method. The PowerPlex® 18D Kit was used to test 1483 cases and their conclusions of paternity tests were verified. Results: In the 1483 cases, 1047 were parental triplet and 436 were uniparental diad. A total of 2530 times of meiosis was observed. One STR locus mutation was observed in 24 cases. And 11 mutation loci were found in the 17 STR loci. Conclusion: STR loci mutation is a common phenomenon. We should collect the data of STR loci mutation, choose other good polymorphism, low mutation rate of genetic markers, to ensure that the results are accurate and reliable.


PubMed | Yanan Hospital of Kunming and The First Peoples Hospital of Qujing City
Type: Journal Article | Journal: Journal of thrombosis and thrombolysis | Year: 2016

The expression of let-7 family members was differentiated in ischemic stroke (IS), functioning as an important regulating molecular in the pathophysiology of stroke. We hypothesized that genetic polymorphism in the promoters of let-7 family may be associated with the risk of IS. To test this hypothesis, we investigated the association of the rs10877887 and rs13293512 in the promoters of let-7 family with the susceptibility to IS. A hospital-based case-control study was performed. The rs10877887 genotype was determined by using a polymerase chain reaction-restriction fragment length polymorphism assay, and the rs13293512 genotype was determined by using a TaqMan assay. We found that the rs13293512CC genotype was associated with a reduced risk of IS (CC vs. TT: adjusted OR=0.43, 95% CI 0.26-0.71; dominant model: adjusted OR=0.70, 95% CI 0.49-0.98; recessive model: adjusted OR=0.45, 95% CI, 0.28-0.73). Stratification analysis showed that the rs10877887TT carriers had a higher level of total cholesterol compared to rs10877887TC/CC carriers (P=0.03). Combined analysis showed that the rs10877887TC/CC and rs13293512TC/CC genotypes had a reduced risk of IS risk (adjusted OR=0.58, 95% CI 0.36-0.95). Our findings suggest that the rs13293512 polymorphism may be a protective factor for the development of IS.

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