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Kunming, China

Hu J.,The First Peoples Hospital of Kunming | Shu J.,Kunming Medical University | Yuan X.,The First Peoples Hospital of Kunming | Nie B.,The First Peoples Hospital of Kunming | And 4 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Objective: To explore transplanting autologous bone marrow mesenchymal stem cells (MSCs) compounded with “two-phase” autologous bone matrix gelatin (BMG) into the defects after surgical removal of rabbit intervertebral disc and the feasibility and efficacy of repairing disc. Methods: 48 experimental Japanese rabbits were randomly divided into control group (n=24) and experimental group (n=24). After rabbit models of total removing intervertebral disc were made, experimental group was implanted with autogeneic cell-carrier complex, while control group was only implanted with autologous BMG. Then these rabbits were sacrificed at the 8th and 12th week. Specimens were obtained and underwent general observation and HE staining. Sulfuric acid-carbazole method was used to assay aggrecan, while immunohistochemistry and RT-PCR were performed to detect Collagen II. Results: Intervertebral discs of rabbits in experimental group were repaired with the growth of cartilage endplate and fibrocartilage tissue, while repairing discs in control group was not as good as that in experimental group. Moreover, levels of aggrecan and Collagen II of cartilage endplate and fibrocartilage tissue in experimental group were significantly increased when compared with that in control group (P<0.05) and the difference was statistically significant. Conclusion: MSCs can repair the rabbit inverterbral disc with the growth of cartilage endplate and fibrocartilage tissue. © 2016, E-Century Publishing Corporation. All rights reserved.

Dong Y.,First Affiliated Hospital of Kunming Medical College | Kang B.,Yanan Hospital of Kunming | Ding P.,First Affiliated Hospital of Kunming Medical College | Dai X.-s.,First Affiliated Hospital of Kunming Medical College | And 4 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2012

BACKGROUND: Studies have shown that the chemokine stromal cell derived factor-1 and vascular endothelial growth factor have the ability to make neural stem cells (NSCs) migration. OBJECTIVE: To explore the effect of monocyte chemoattractant protein 1 (MCP-1) and its receptoron the migrati on of NSCs in vitro. METHODS: Hippocampal NSCs from SD fetal rats were isolated and cultured; meanwhile they could be amplified and identified in vitro; The expression of CCR2 was detected with immunofluorescence and RT-PCR; Effect of MCP-1 with different concentrations (50, 100, 200, 300, 500 ng/mL) on NSCs in vitro migration was obsened under agarose. RESULTS AND CONCLUSION: Immunofluorescence and RT-PCR results showed that NSCs from fetal rat hippocampal could express chemokine receptor CCR2; and the migration experiment under agarose showed that the MCP-1 with different concentrations could induce the migration of NSCs in vitro and with the higher the dose the better the migration. But the anti-CCR42 polyclonal antibodies could inhibit the migration of NSCs.

Shuai L.,Yanan Hospital of Kunming | Jing Q.,Kunming Medical University
Journal of Forensic Medicine | Year: 2014

Objective: To observe and analyze the mutation phenomenon of 17 STR loci of PowerPlex® 18D Kit in paternity test of Yunnan population. Methods: The DNA was extracted by Chelex-100 method. The PowerPlex® 18D Kit was used to test 1483 cases and their conclusions of paternity tests were verified. Results: In the 1483 cases, 1047 were parental triplet and 436 were uniparental diad. A total of 2530 times of meiosis was observed. One STR locus mutation was observed in 24 cases. And 11 mutation loci were found in the 17 STR loci. Conclusion: STR loci mutation is a common phenomenon. We should collect the data of STR loci mutation, choose other good polymorphism, low mutation rate of genetic markers, to ensure that the results are accurate and reliable.

Li Y.,Kunming Medical University | Yao Y.,Peking Union Medical College | Qian X.,Yanan Hospital of Kunming | Shi L.,Peking Union Medical College | And 2 more authors.
PLoS ONE | Year: 2015

Recently, in vitro studies have demonstrated that adiponectin has antiangiogenic and tumor growth-limiting properties. Additionally, serum adiponectin levels have been associated with the risk of several cancers; specifically, serum adiponectin was significantly lower in lung cancer patients with advanced-stage disease. In this study, we examined the association of adiponectin gene promoter variations associated with adiponectin gene expression and plasma levels in non-small cell lung cancer (NSCLC) in a Han Chinese population. A total of 319 patients with NSCLC and 489 healthy individuals were recruited to evaluate the association of four adiponectin gene promoter single-nucleotide polymorphisms (SNPs) (SNP-12140G>A, SNP-11426A>G, SNP-11391G>A and SNP-11377C>G) with NSCLS risk. Additionally, we constructed haplotypes of these four SNPs and evaluated the association of these haplotypes with NSCLS risk. Our results showed that among these four SNPs, only SNP-12140G>A was associated with NSCLC risk(P<0.05). The haplotype analysis showed that no haplotype was associated with NSCLC after performing a Bonferroni correction (P>0.05). Additionally, an association analysis of the four SNPs stratified into pathologic stages I+II and III+IV showed that these SNPs did not exhibit significant differences between pathologic stages I+II and III+IV. Moreover, we did not observe any differences in allele and genotype frequency for these SNPs between adenocarcinoma and squamous cell carcinoma. Our results indicated that the G allele of SNP-12140may be a risk factor for NSCLC (OR = 1.516; 95% CI: 1.098-2.094) in this Han Chinese population. © 2015 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Zhou S.,Central South University | Zhou S.,Hunan University | Wang B.,Central South University | Hu J.,Central South University | And 5 more authors.
Tumor Biology | Year: 2016

MicroRNAs (miRNAs) are a new class of prognostic and diagnostic biomarkers for many cancers. Recent studies have shown that miRNAs are highly stable in plasma/serum. The aim of this study was to investigate the role of miR-421 in osteosarcoma. We found that the serum expression levels of miR-421 were significantly higher in osteosarcoma patients than those in healthy volunteers. Moreover, miR-421 expression was significantly higher in osteosarcoma tissues compared with that in the adjacent normal tissues. Meanwhile, the expression of miR-421 was upregulated in 90 % (36/40) osteosarcoma tissues compared to non-tumor tissues. More importantly, the expression levels of miR-421 in osteosarcoma tissues were correlated with those in patients’ serum. In addition, patients with high miR-421 expression had shorter overall survival (OS) than those with low expressions. We also found that overexpression of miR-421 promoted osteosarcoma cell line MG-63 proliferation, migration, and invasion. In conclusion, miR-421 expression levels were upregulated in osteosarcoma tissue and serum and it may be a useful marker for diagnosis of osteosarcoma. © 2016 International Society of Oncology and BioMarkers (ISOBM)

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