Yamaguchi-shi, Japan
Yamaguchi-shi, Japan

Yamaguchi Prefectural University is a public university in Yamaguchi, Yamaguchi, Japan. The predecessor of the school was founded in 1941, and it was chartered as a university in 1975. In 1996 it became coeducative. Wikipedia.


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Matsui K.,Yamaguchi University | Takaki S.,Yamaguchi University | Shimada K.,Yamaguchi Prefectural University | Hajika M.,Japan National Agriculture and Food Research Organization
Bioscience, Biotechnology and Biochemistry | Year: 2011

Oxygenation of lipids during the processing soybeans affects the flavor properties of soy products. We prepared tofu under anaerobic conditions and then evaluated its sensory properties and the compositions of volatiles and oxidized lipids. Anaerobic processing resulted in tofu with less intense richness (kokumi) concomitant with reductions in the amounts of oxidized lipids and volatile compounds.


Kumamaru T.,Kyushu University | Uemura Y.,Kyushu University | Inoue Y.,Kyushu University | Takemoto Y.,Kyushu University | And 6 more authors.
Plant and Cell Physiology | Year: 2010

To identify the function of genes that regulate the processing of proglutelin, we performed an analysis of glup3 mutants, which accumulates excess amounts of proglutelin and lack the vacuolar processing enzyme (VPE). VPE activity in developing seeds from glup3 lines was reduced remarkably compared with the wild type. DNA sequencing of the VPE gene in glup3 mutants revealed either amino acid substitutions or the appearance of a stop codon within the coding region. Microscopic observations showed that α-globulin and proglutelin were distributed homogeneously within glup3 protein storage vacuoles (PSVs), and that glup3 PSVs lacked the crystalline lattice structure typical of wild-type PSVs. This suggests that the processing of proglutelin by VPE in rice is essential for proper PSV structure and compartmentalization of storage proteins. Growth retardation in glup3 seedlings was also observed, indicating that the processing of proglutelin influences early seedling development. These findings indicate that storage of glutelin in its mature form as a crystalline structure in PSVs is required for the rapid use of glutelin as a source of amino acids during early seedling development. In conclusion, VPE plays an important role in the formation of protein crystalline structures in PSVs.


Yoshimura K.,Yamaguchi University | Yoshimura K.,Yamaguchi Prefectural University | Aoki H.,Kurume University
International Journal of Vascular Medicine | Year: 2012

Abdominal aortic aneurysm (AAA) is a common disease causing segmental expansion and rupture of the aorta with a high mortality rate. The lack of nonsurgical treatment represents a large and unmet need in terms of pharmacotherapy. Advances in AAA research revealed that activation of inflammatory signaling pathways through proinflammatory mediators shifts the balance of extracellular matrix (ECM) metabolism toward tissue degradation. This idea is supported by experimental evidence in animal models that pharmacologic intervention at each pathological step can prevent AAA development. Previously, we identified c-Jun N-terminal kinase (JNK), a pro-inflammatory signaling molecule, as a therapeutic target for AAA. Abnormal activation of JNK in AAA tissue regulates multiple pathological processes in a coordinated manner. Pharmacologic inhibition of JNK tips the ECM balance back towards repair rather than degradation. Interventions targeting signaling molecules such as JNK in order to manipulate multiple pathological processes may be an ideal therapeutic strategy for AAA. Furthermore, the development of biomarkers as well as appropriate drug delivery systems is essential to produce clinically practical pharmacotherapy for AAA. © 2012 Koichi Yoshimura and Hiroki Aoki.


Washida H.,Washington State University | Washida H.,Nara Institute of Science and Technology | Sugino A.,Washington State University | Sugino A.,Nara Institute of Science and Technology | And 9 more authors.
Plant Journal | Year: 2012

Studies focusing on the targeting of RNAs that encode rice storage proteins, prolamines and glutelins to specific sub-domains of the endoplasmic reticulum (ER), as well as mis-localization studies of other storage protein RNAs, indicate a close relationship between the ER site of RNA translation and the final site of protein deposition in the endomembrane system in developing rice endosperm. In addition to prolamine and glutelin, rice accumulates smaller amounts of α-globulins, which are deposited together with glutelin in the protein storage vacuole (PSV). In situ RT-PCR analysis revealed that α-globulin RNAs are not distributed to the cisternal ER as expected for a PSV-localized protein, but instead are targeted to the protein body-ER (PB-ER) by a regulated process requiring cis-sorting sequences. Sequence alignments with putative maize Î-zein cis-localization elements identified several candidate regulatory sequences that may be responsible for PB-ER targeting. Immunocytochemical analysis confirmed the presence of α-globulin on the periphery of the prolamine protein bodies and packaging in Golgi-associated dense vesicles, as well as deposition and storage within peripheral regions of the PSV. Mis-targeting of α-globulin RNAs to the cisternal ER dramatically alters the spatial arrangement of α-globulin and glutelin within the PSV, with the accompanying presence of numerous small α-globulin particles in the cytoplasm. These results indicate that α-globulin RNA targeting to the PB-ER sub-domain is essential for efficient transport of α-globulins to the PSV and its spatial arrangement in the PSV. Such RNA localization prevents potential deleterious protein-protein interactions, in addition to performing a role in protein targeting. © 2011 Blackwell Publishing Ltd.


Nagamine A.,Kyushu University | Matsusaka H.,Kyushu University | Ushijima T.,Kyushu University | Kawagoe Y.,Japan National Institute of Agrobiological Science | And 3 more authors.
Plant and Cell Physiology | Year: 2011

The rice prolamins consist of cysteine-rich 10 kDa (CysR10), 14 kDa (CysR14) and 16 kDa (CysR16) molecular species and a cysteine-poor 13 kDa (CysP13) polypeptide. These storage proteins form protein bodies (PBs) composed of single spherical intracisternal inclusions assembled within the lumen of the rough endoplasmic reticulum. Immunofluorescence and immunoelectron microscopy demonstrated that CysR10 and CysP13 were asymmetrically distributed within the PBs, with the former concentrated at the electron-dense center core region and the latter distributed mainly to the electron-lucent peripheral region. These results together with temporal expression data showed that the formation of prolamin-containing PB-I in the wild-type endosperm was initiated by the accumulation of CysR10 to form the center core. In mutants deficient for cysteine-rich prolamins, the typical PB-I structures containing the electron-dense center core were not observed, and instead were replaced by irregularly shaped, electron-lucent, hypertrophied PBs. Similar, deformed PBs were observed in a CysR10 RNA interference plant line. These results suggest that CysR10, through its formation of the central core and its possible interaction with other cysteine-rich prolamins, is required for tight packaging of the proteins into a compact spherical structure. © 2011 The Author.


Onda Y.,Japan National Institute of Agrobiological Science | Nagamine A.,Japan National Institute of Agrobiological Science | Sakurai M.,Japan National Institute of Agrobiological Science | Kumamaru T.,Kyushu University | And 2 more authors.
Plant Cell | Year: 2011

In the rice (Oryza sativa) endosperm, storage proteins are synthesized on the rough endoplasmic reticulum (ER), in which prolamins are sorted to protein bodies (PBs) called type-I PB (PB-I). Protein disulfide isomerase (PDI) family oxidoreductase PDIL2;3, an ortholog of human P5, contains a conserved structural disulfide in the redox-inactive thioredoxin-like (TRX) domain and was efficiently targeted to the surface of PB-I in a redox active site-dependent manner, whereas PDIL1;1, an ortholog of human PDI, was localized in the ER lumen. Complementation analyses using PDIL1;1 knockout esp2 mutant indicated that the a and a′ TRX domains of PDIL1;1 exhibited similar redox activities and that PDIL2;3 was unable to perform the PDIL1;1 functions. PDIL2;3 knockdown inhibited the accumulation of Cys-rich 10-kD prolamin (crP10) in the core of PB-I. Conversely, crP10 knockdown dispersed PDIL2;3 into the ER lumen. Glutathione S-transferase-PDIL2;3 formed a stable tetramer when it was expressed in Escherichia coli, and the recombinant PDIL2;3 tetramer facilitated a-globulin(C79F) mutant protein to form nonnative intermolecular disulfide bonds in vitro. These results indicate that PDIL2;3 and PDIL1;1 are not functionally redundant in sulfhydryl oxidations of structurally diverse storage proteins and play distinct roles in PB development. We discuss PDIL2;3-dependent and PDIL2;3-independent oxidation pathways that sustain disulfide bonds of crP10 in PB-I. © American Society of Plant Biologists.


Yamazaki F.,Yamaguchi Prefectural University
Journal of Physiological Sciences | Year: 2015

To clarify the characteristics of the thermal control of skin blood flow (SkBF) in individuals with a cold constitution, we examined the cutaneous vasoconstrictor responses in the calf and dorsal foot during whole-body and local skin cooling in young women complaining of chilliness (C group) and young women not suffering from it (N group). During whole-body skin cooling, the vasoconstrictor sensitivity in the dorsal foot, but not in the calf, was greater in the C group than in the N group. The C group also showed greater vasoconstrictor responses in the dorsal foot, but not in the calf, during local skin cooling and the iontophoretic application of norepinephrine. These findings suggest that the C group possesses a specific SkBF controlling system that is characterized by higher adrenergic sensitivity for greater cutaneous vasoconstriction in the distal portion of the lower extremities during cold exposure. © 2015, The Physiological Society of Japan and Springer Japan.


Yamashita O.,Yamaguchi University | Yoshimura K.,Yamaguchi University | Yoshimura K.,Yamaguchi Prefectural University | Nagasawa A.,Yamaguchi University | And 4 more authors.
PLoS ONE | Year: 2013

Aims: Abdominal aortic aneurysms (AAAs) are characterized by chronic inflammation, which contributes to the pathological remodeling of the extracellular matrix. Although mechanical stress has been suggested to promote inflammation in AAA, the molecular mechanism remains uncertain. Periostin is a matricellular protein known to respond to mechanical strain. The aim of this study was to elucidate the role of periostin in mechanotransduction in the pathogenesis of AAA. Methods and Results: We found significant increases in periostin protein levels in the walls of human AAA specimens. Tissue localization of periostin was associated with inflammatory cell infiltration and destruction of elastic fibers. We examined whether mechanical strain could stimulate periostin expression in cultured rat vascular smooth muscle cells. Cells subjected to 20% uniaxial cyclic strains showed significant increases in periostin protein expression, focal adhesion kinase (FAK) activation, and secretions of monocyte chemoattractant protein-1 (MCP-1) and the active form of matrix metalloproteinase (MMP)-2. These changes were largely abolished by a periostin-neutralizing antibody and by the FAK inhibitor, PF573228. Interestingly, inhibition of either periostin or FAK caused suppression of the other, indicating a positive feedback loop. In human AAA tissues in ex vivo culture, MCP-1 secretion was dramatically suppressed by PF573228. Moreover, in vivo, periaortic application of recombinant periostin in mice led to FAK activation and MCP-1 upregulation in the aortic walls, which resulted in marked cellular infiltration. Conclusion: Our findings indicated that periostin plays an important role in mechanotransduction that maintains inflammation via FAK activation in AAA. © 2013 Yamashita et al.


Yamashita T.,Yamaguchi Prefectural University | Hayashi T.,Yamaguchi Prefectural University
No To Hattatsu | Year: 2012

Guidelines for the diagnosis of reading disorders in elementary school students were published recently in Japan. On the basis of these guidelines, we administrated reading test batteries to 43 Japanese junior high-school students from grade two. The reading test consisted of single sounds, single words, and single sentences. We evaluated the reading speed and the number of reading errors made by the test takers; their performance was compared with the normal value for elementary school students in grade six, as stated in the guidelines. The reading ability of the junior high-school students was not higher than that of the elementary school students. Seven students (16.3%) were found to have reading difficulties (RD group) and they met the criterion for diagnosis of reading disorder as per the guidelines. Three students had difficulties in reading single sounds and single words, but they faced no problems when reading single sentences. It was supposed that the strategies used by the students for reading sentences may have differed from those used for reading single sounds or single words. No significant differences were found between the RD and non-RD group students on scores of scholastic self-evaluation, self-esteem, and depressive symptoms. Therefore, reading difficulty did not directly influence the level of self-evaluation or depression.


Tan Y.,Yamaguchi Prefectural University
[Nihon kōshū eisei zasshi] Japanese journal of public health | Year: 2011

To establish an effective distribution method and necessary content for a pamphlet on pediatric emergencies for parents and guardians of kindergarteners by studying the possession rate of a pamphlet and impressions and opinions about its content. The number of subjects was 269. The pamphlet was delivered via kindergartens to parents and guardians; two months later, an anonymous questionnaire was sent inquiring about their possession rate and impressions and opinions about the content of the pamphlet. The A6 20-page all-color pamphlet (designed by the author) comprised questions regarding their decision to take their children to the hospital for 6 symptoms (fever, seizures, vomiting, diarrhea, cough, and abdominal pain), knowledge about physical assessment methods and homecare methods for children, and the phone numbers and URLs of "24-hour emergency lines for children." The number of valid responses was 111 (41.3%) (mean age: 36.0 +/- 4.50), with 74 (66.7%) retaining the pamphlet. There were significant differences in their experiences of illness in their children (P < 0.05) between the possession group and disposed of group, the percentage being higher in the former. Among the subjects who retained the pamphlet, 67 (90.5%) had read through it. Among them, 51 subjects (76.1%) answered that it had useful pages, and 63 subjects (94.0%) answered that they would use it in the future. Out of the information provided, including the 6 symptoms, frequently asked questions, records, and a list of phone numbers and URLs, the most useful information according to them was "a list of phone numbers and URLs" (28/54.9%), followed by "the decision to take the children to the hospital for fever" (24/47.1%) and "frequently asked questions" (21/41.2%). The possession rate was significantly higher in subjects who had experienced sudden illnesses in their children. Therefore, the likelihood of the pamphlet being retained and used would be higher if delivered to parents and guardians with children of an age more susceptible to sudden illnesses. Furthermore, many respondents chose "a list of phone numbers and URLs" as the most useful information. Therefore, a pamphlet that demonstrates how necessary information can be easily obtained and used may be more practical than one containing all the information.

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