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Shizuoka-shi, Japan

Shigemura Y.,Tokyo Kasei University | Kubomura D.,Yaizu Suisankagaku Industry Co. | Sato Y.,Tokyo Kasei University | Sato K.,Kyoto Prefectural University
Food Chemistry | Year: 2014

The presence of hydroxyproline (Hyp)-containing peptides in human blood after collagen hydrolysate ingestion is believed to exert beneficial effects on human health. To estimate the effective beneficial dose of these peptides, we examined the relationship between ingested dose and food-derived Hyp levels in human plasma. Healthy volunteers (n = 4) ingested 30.8, 153.8 and 384.6 mg per kg body weight of collagen hydrolysate. The average plasma concentration of Hyp-containing peptides was dose-dependent, reaching maximum levels of 6.43, 20.17 and 32.84 nmol/ml following ingestion of 30.8, 153.8 and 384.6-mg doses of collagen hydrolysate, respectively. Ingesting over 153.8 mg of collagen hydrolysate significantly increased the average concentrations of the free and peptide forms of Hyp in plasma. The Hyp absorption limit was not reached with ingestion of as much as 384.6 mg of collagen hydrolysate. These finding suggest that ingestion of less than 30.8 mg of collagen hydrolysate is not effective for health benefits. © 2014 Elsevier Ltd. All rights reserved. Source


Uno K.,Aichi Konan College | Higashioto Y.,Yaizu Suisankagaku Industry Co. | Chaweepack T.,Chanthaburi Coastal Fisheries Research and Development Center | Ruangpan L.,Ministry of Agriculture and Cooperative
Turkish Journal of Fisheries and Aquatic Sciences | Year: 2012

The present study examined the influences of industrial chitin extraction processes on the residual oxytetracycline (OTC) and oxolinic acid (OA) in shrimp carapaces and shells. The drugs were orally administered by catheter to the kuruma shrimp (Penaeus japonicus) and vannamei shrimp (Penaeus vannamei). The shrimps were sampled at 6-h post-dosing and their carapaces and shells were collected and used as raw material in the chitin extraction. Residual OTC levels in raw materials were 9 - 18 fold higher than the maximum residue limit (MRL) (0.2 μg/g). The residual OTC was reduced 10 - 30% by washing treatment. By the acid treatment with HCl, the OTC residues were reduced >98%. The deproteinized material contained negligible levels of OTC. Residual OA levels in raw materials was much higher (134 - 376 fold) than the MRL (0.03 μg/g). The residual OA was reduced considerably (34 - 68%) by washing treatment. By the demineralization, the residual OA could not be detected in both shrimp carapaces, while the levels of OA residues were determined more than MRL in shells. In the deproteinized materials, no residual OA were detected. Finally, residual OTC and OA could not be detected in the chitin materials for both shrimps. © Published by Central Fisheries Research Institute (CFRI) Trabzon, Turkey in cooperation with Japan International Cooperation Agency (JICA), Japan. Source


Shintani T.,Kyoto University | Yamazaki F.,Kyoto University | Katoh T.,Kyoto University | Umekawa M.,Kyoto University | And 6 more authors.
Biochemical and Biophysical Research Communications | Year: 2010

Autophagy is a cellular process that nonspecifically degrades cytosolic components and is involved in many cellular responses. We found that amino sugars with a free amino group such as glucosamine, galactosamine and mannosamine induced autophagy via an mTOR-independent pathway. Glucosamine-induced autophagy at concentrations of at least 500 μM to over 40 mM. In the presence of 40 mM glucosamine, autophagy induction was initiated at 6 h and reached a plateau at 36 h. Glucosamine-induced autophagy could remove accumulated ubiquitin-conjugated proteins as well as 79-glutamine repeats. Therefore, orally administered glucosamine could contribute to the prevention of neurodegenerative diseases and promotion of antiaging effects. © 2010 Elsevier Inc. All rights reserved. Source


Kubomura D.,Yaizu Suisankagaku Industry Co. | Matahira Y.,Yaizu Suisankagaku Industry Co. | Nagai K.,Osaka University | Nagai K.,ANBAS Corporation | Niijima A.,Niigata University
Nutritional Neuroscience | Year: 2010

Anserine and L-carnosine are similar dipeptides synthesized by muscles of vertebrates. The functional role of anserine is unknown, although previous studies showed hypoglycemic effects of carnosine through autonomic nerves. Thus, we evaluated the effects of anserine on blood glucose levels and neural activities. Intraperitoneal administration of specific doses of anserine to hyperglycemic rats reduced hyperglycemia and plasma glucagon concentrations, whereas thioperamide eliminated the effects of anserine. Intraduodenal injection of 0.1 mg anserine to anesthetized rats after laparotomy suppressed sympathetic nerve activity and enhanced activity of the vagal gastric efferent. In addition, oral administration of anserine reduced blood glucose levels during oral glucose tolerance testing in humans. These results suggest the possibility that anserine might be a control factor for blood glucose, and that histaminergic nerves may be involved in the hypoglycemic effects of anserine. © 2010 W. S. Maney & Son Ltd. Source


Kubomura D.,Yaizu Suisankagaku Industry Co. | Matahira Y.,Yaizu Suisankagaku Industry Co. | Nagai K.,Osaka University | Nagai K.,ANBAS Corporation | Niijima A.,Niigata University
Nutritional Neuroscience | Year: 2010

Anserine and L-carnosine are similar dipeptides synthesized by muscles of vertebrates. The functional role of anserine is unknown, although previous studies showed hypoglycemic effects of carnosine through autonomic nerves. Thus, we evaluated the effects of anserine on blood glucose levels and the neural activities. Intraperitoneal administration of specific doses of anserine to hyperglycemic rats reduced hyperglycemia and plasma glucagon concentrations, whereas thioperamide eliminated the effects of anserine. Intraduodenal injection of 0.1 mg anserine to anesthetized rats after laparotomy suppressed sympathetic nerve activity and enhanced activity of the vagal gastric efferent. In addition, oral administration of anserine reduced blood glucose levels during oral glucose tolerance testing in humans. These results suggest the possibility that anserine might be a control factor for the blood glucose, and that histaminergic nerves may be involved in the hypoglycemic effects of anserine. © 2010 W. S. Maney & Son Ltd. Source

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