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Song C.,Sichuan Agricultural University | Song C.,Yaan Vocational College | Liu H.,Sichuan Agricultural University | Kou J.,Sichuan Agricultural University | And 4 more authors.
Genetics and Molecular Research | Year: 2013

Insulin-like growth factors (IGFs) are regulators that modulate the proliferation and differentiation of muscle tissues. We quantified the messenger RNA (mRNA) expression of IGF-I, IGF-II, and type I and II IGF receptors (IGF-IR and IGF-IIR) in muscle tissues including the breast, leg, and myocardium during an early postnatal development growth stage (post-hatching weeks 1-8) in ducks. The results showed a significant age-related change in mRNA in these muscle tissues. In breast muscle, the developmental expression of IGF-I and IGF-II was highest during week 1 but decreased quickly and maintained a relatively lower level. Leg muscle had the highest mRNA expression of IGF-I and IGF-II genes at week 3. In myocardial tissues, the expression level of IGF-IR and IGF-IIR genes exhibited a "rise-decline" developmental trend. The expression patterns of IGF-I/IGF-IR and IGF-II/IGF-IIR were different between weeks 4 and 6. The same expression pattern was observed for IGF-I and IGF-IR; however, it was different from that observed for IGF-II and IGF-IIR. Our results showed a negative correlation between IGF-II mRNA expression and leg muscle weight at week 4 (P < 0.05). A negative correlation was also found between IGF-II mRNA expression and breast muscle weight (P <0.01), and a positive correlation was found between IGF-IR expression and breast muscle weight. At week 6, a positive correlation was found between IGF-IR expression and breast muscle weight. However, at week 8, a negative correlation was found between IGF-IR expression and breast muscle weight. The results showed that the expression of IGF mRNA in duck tissues exhibits a specific developmental trend and an age-related pattern, suggesting that the regulation mechanism of these 4 genes in proliferation and differentiation of muscle tissues differed. © FUNPEC-RP. Source


Fu Y.-K.,Southwest Jiaotong University | Fu Y.-K.,Yaan Vocational College | Zhou X.,Southwest Jiaotong University | Xiao D.-Q.,Southwest Jiaotong University | And 3 more authors.
Wuji Cailiao Xuebao/Journal of Inorganic Materials | Year: 2015

Hydroxyapatite (HAP) particles were hydrothermally synthesized with the surface morphologies adjusted by cyclohexane-1, 2, 3, 4, 5, 6-hexacarboxylic acid (H6E) as the template. HAP particles were characterized by XRD, BET, SEM and FTIR. The protein adsorption-desorption behaviors of positively charged lysozyme (LYS), fibrinogen (FN) and negatively charged bovine serum albumin (BSA) on these HAP particles were examined. The results indicate that using H6E as a template to fabricate micro-nano structures on HAP particles through hydrothermal reaction is simple and controllable. HAP particles with micro-nano structures show selective protein adsorption-desorption properties for different proteins. The protein-loaded shell-like HAP particle (HAP50-protein) shows an excellent protein release behavior in vitro. ©, 2015, Science Press. All right reserved. Source


Zhou Q.,Yaan Vocational College | Li M.,Sichuan Agricultural University | Wang X.,Sichuan Agricultural University | Li Q.,Sichuan Agricultural University | And 6 more authors.
International Journal of Biological Sciences | Year: 2011

Breast milk is a complex liquid rich in immunological components that affect the development of the infant's immune system. Exosomes are membranous vesicles of endocytic origin that are found in various body fluids and that can mediate intercellular communication. Mi-croRNAs (miRNAs), a well-defined group of non-coding small RNAs, are packaged inside exosomes in human breast milk. Here, we identified 602 unique miRNAs originating from 452 miRNA precursors (pre-miRNAs) in human breast milk exosomes using deep sequencing technology. We found that, out of 87 well-characterized immune-related pre-miRNAs, 59 (67.82%) are presented and enriched in breast milk exosomes (P < 10 -16, χ 2 test). In addition, compared with exogenous synthetic miRNAs, these endogenous immune-related miRNAs are more resistant to relatively harsh conditions. It is, therefore, tempting to speculate that these exosomal miRNAs are transferred from the mother's milk to the infant via the digestive tract, and that they play a critical role in the development of the infant immune system. © Ivyspring International Publisher. Source


Li M.,Sichuan Agricultural University | Li M.,Tsinghua University | Tian S.,Novogene Bioinformatics Institute | Jin L.,Sichuan Agricultural University | And 49 more authors.
Nature Genetics | Year: 2013

We report the sequencing at 131× coverage, de novo assembly and analyses of the genome of a female Tibetan wild boar. We also resequenced the whole genomes of 30 Tibetan wild boars from six major distributed locations and 18 geographically related pigs in China. We characterized genetic diversity, population structure and patterns of evolution. We searched for genomic regions under selection, which includes genes that are involved in hypoxia, olfaction, energy metabolism and drug response. Comparing the genome of Tibetan wild boar with those of neighboring Chinese domestic pigs further showed the impact of thousands of years of artificial selection and different signatures of selection in wild boar and domestic pig. We also report genetic adaptations in Tibetan wild boar that are associated with high altitudes and characterize the genetic basis of increased salivation in domestic pig. Source


Yang J.-W.,Zunyi Medical University | Nie X.-Q.,Zunyi Medical University | Nie X.-Q.,Shanghai University of Traditional Chinese Medicine | Shi H.-X.,Shanghai JiaoTong University | And 5 more authors.
Zhongguo Zhongyao Zazhi | Year: 2014

II is now well established that inflammation plays an important role in the development of numerous chronic metallic diseases including insulin resistance (IR) and tyjx1 2 diabetes (T2DM). Skeletal muscle is responsible for 75% of total insnlin-de- pendent glucose uptake; consequently, skeletal muscle IR is considered to be the primary defect of systemic IR development. Our previous study has shown that rutaecarpine (Rut) can benefit blood lipid profile, mitigate inflammation, and improve kidney, liver, pancreas pathology status of T2DM rats. However, the effects of Rut on inflammatory cytokines in the development of IR-skeletal muscle cells have not been studied. Thus, our objective was to investigate effects of Rut on inflammatory cytokines interleukin (IL)-l , 11,-6 and tumor necrosis factor (TNF)-α in insulin resistant primary skeletal muscle cells (IR-PSMC). Primary cultures of skeletal muscle cells were prepared from 5 neonate SI) rats, ; nd the primary rat skeletal muscle cells were identified by cell morphology, effect of rutaecarpine on cell proliferation by MTT assay. IR-PSMC cells were induced by palmitic acid (PA), the glucose concentration was measured by glucose oxidase and peroxidase (GOD-POD) method. The effects of Rut on inflammatory cytokines IL-1 , 11,-6 and TNF-a in IR-PSMC cells were tested by enzyme-linked immunosorbent assay ( FJ.ISA) kit. The results show that the primary skeletal muscle cells from neonatal rat cultured for 2-4 days, parallel alignment regularly, and cultured for 7 days, cells fused and myotube formed. It was shown that Rut in concentration 0-180. 0 jjliuoI L-1 possessed no cytotoxic effect towards cultured primary skeletal muscle cells. However, after 24 h exposure to 0. 6 mmol L-1 PA, primary skeletal muscle cells were able to induce a state of insulin resistance. The results obtained indicated significant decrease (P<0. 05 to P <0.001) IL-1 , IL-6 and TNF-a production by cultured IR-PSMC cells when incubating 24 hours with Rut, beginning from 20 to 180. 0 moloI L -1. IL-1 , IL-6 and TNF-a in the Rut treated groups were dose-dej>endently decreased compared with that in the IR-PSMC control group. Our results demonstrated that the Rut promoted glucose consumption and improved insulin resistance possibly through suppression of inflammatory cytokines in the IR-PSMC cells. Source

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