Zhao X.,Xuzhou Medical College
Endocrine Journal | Year: 2014
Strategies for increasing functional beta cell mass are effective for diabetes therapy. Although controversy remains on the existence of facultative beta cell progenitors in the adult pancreas, most evidence does not support such a possibility. One of the greatest physiological increases in beta cells has been detected in the maternal pancreas during pregnancy, following neonatal period and in the setting of insulin resistance. However, no systematical analysis on the beta cell growth in this period has been ever performed. Here we analyzed beta cell replication by quantifying BrdU incorporated beta cells at different time points in the pregnant mice. Similarly, we evaluated the possible involvement of beta cell neogenesis (differentiation from progenitor cells) by analyzing expression of Neurog3, a key determinant of pancreatic endocrine cell neogenesis during embryogenesis, in the exocrine pancreas. We found a dynamic increase in beta cell replication, but failed to detect beta cell neogenesis, demonstrating that beta cell growth in the maternal pancreas during pregnancy is predominantly attributable to beta cell replication, rather than beta cell neogenesis. © The Japan Endocrine Society.
Liu L.M.,Xuzhou Medical College
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012
To investigate the change of Th22 cells in the peripheral blood of the patients with primary immune thrombocytopenia (ITP) and evaluate the significance of Th22 cells in ITP. The proportion of Th22 cells in the peripheral blood of ITP patients before therapy (group 1), ITP patients in complete response after therapy (ITP-CR, group 2) and healthy donors (group 3) was evaluated by flow cytometry. The cytokines IL-22, TGF-β, TNF-α and IL-6 of each group were measured by ELISA. The level of IL-22 mRNA of each group was examined by RT-PCR. The proportion of Th22 cells and the levels of IL-22, TNF-α, IL-6 and IL-22 mRNA in group l and group 2 were significantly higher than those in group 3 (P<0.01). The proportion of Th22 cells and the levels of IL-22, TNF-α, IL-6 and IL-22 mRNA in group 2 were lower than those in group 1(P<0.05). But the level of TGF-β in group l [(3.27±1.02) ng/L] and group 2 [(5.41±1.69) ng/L] was significantly lower than that in group 3 [(9.65±2.78) ng/L] (P<0.01), and the level of TGF-β in group 1 was lower than that in group 2 (P<0.05). In ITP patients, the number of Th22 cells and the levels of TNF-α and IL-6 increase, and the level of TGF-β decrease.
Qian Y.,East China Institute of Technology |
Wang C.,East China Institute of Technology |
Gao F.,Xuzhou Medical College
Biosensors and Bioelectronics | Year: 2015
A new strategy to combine Zn2+ assistant DNA recycling followed with hybridization chain reaction dual amplification was designed for highly sensitive electrochemical detection of target DNA. A gold electrode was used to immobilize molecular beacon (MB) as the recognition probe and perform the amplification procedure. In the presence of the target DNA, the hairpin probe 1 was opened, and the DNAzyme was liberated from the caged structure. The activated DNAzyme hybridized with the MB and catalyzed its cleavage in the presence of Zn2+ cofactor and resulting in a free DNAzyme strand. Finally, each target-induced activated DNAzyme underwent many cycles triggering the cleavage of MB, thus forming numerous MB fragments. The MB fragments triggered the HCR and formed a long double-helix DNA structure. Because both H1 and H2 were labeled by biotin, a lot of SA-ALP was captured on the electrode surface, thus catalyzing a silver deposition process for electrochemical stripping analysis. This novel cascade signal amplification strategy can detect target DNA down to the attomolar level with a dynamic range spanning 6 orders of magnitude. This highly sensitive and specific assay has a great potential to become a promising DNA quantification method in biomedical research and clinical diagnosis. © 2014 Elsevier B.V.
Tollini L.A.,University of North Carolina at Chapel Hill |
Jin A.,University of North Carolina at Chapel Hill |
Park J.,University of North Carolina at Chapel Hill |
Zhang Y.,University of North Carolina at Chapel Hill |
Zhang Y.,Xuzhou Medical College
Cancer Cell | Year: 2014
Mdm2 E3 ubiquitin ligase-mediated p53 degradation is generally accepted as the major mechanism for p53 regulation; nevertheless, the invivo significance of this function has not been unequivocally established. Here, we have generated an Mdm2Y487A knockin mouse; Mdm2Y487A mutation inactivates Mdm2 E3 ligase function without affecting its ability to bind its homolog MdmX. Unexpectedly, Mdm2Y487A/Y487A mice were viable and developed normally into adulthood. While disruption of Mdm2 E3 ligase function resulted in p53 accumulation, p53 transcriptional activity remained low; however, exposure to sublethal stress resulted in hyperactive p53 and p53-dependent mortality in Mdm2Y487A/Y487A mice. These findings reveal a potentially dispensable nature for Mdm2 E3 ligase function in p53 regulation, providing insight that may affect how this pathway is targeted therapeutically. © 2014 Elsevier Inc.
Li J.,Xuzhou Medical College |
Zhu J.-J.,Nanjing University |
Xu K.,Xuzhou Medical College
TrAC - Trends in Analytical Chemistry | Year: 2014
Fluorescent metal nanoclusters (NCs) are a class of emerging fluorescent materials. They have excellent photostability and biocompatibility with sub-nanometer size and are easy to synthesize. Taking advantage of these features, fluorescent metal NCs have been involved in exciting developments of analytical methods for fluorescent biosensing and bioimaging. In this review, we first summarize the approaches to synthesis and bioconjugation for fluorescent metal NCs (Ag, Au, Cu and Pt). We then highlight their applications as fluorescent probes for metal ions, small molecules, nucleic acids, and protein detection. We also summarize the use of metal NCs in cellular and in-vivo targeting and imaging. Finally, we envision the various prospects for research on fluorescent metal NCs in the future. © 2014 Elsevier Ltd.