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Liang J.,Xuzhou Medical College | Liang J.,Xuzhou Institute of Medical science | Pei Y.,Nanjing Southeast University | Gong Y.,Xuzhou Medical College | And 11 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2015

OBJECTIVE: The causal relationship between serum uric acid (SUA) level and non-alcoholic fatty liver disease (NAFLD) has not yet been clarified. The objective of the study was to determine the association between SUA and NAFLD, as well as assess the interactions between SUA and other metabolic risk factors regarding NAFLD. PATIENTS AND METHODS: The study samples related to a community-based health examination survey conducted in Central China. Initially, a total of 24,878 patients with medical examination were included. After excluding the individuals with confounding factors, the remaining 21,798 subjects with biomarkers available were included in the present study. RESULTS: The data show that the risk of NAFLD significantly increased with the elevated SUA levels. Further adjustments for sex, age, and other confounding metabolic factors did not change the increasing trend of NAFLD risk. The odds ratios [ORs, 95% confidence interval (CI)] of NAFLD across the increasing quintiles of SUA were 1.00, 1,530 (1.174-1.995), 2.24 (1.714-2.886), 2.636 (2.019-3.441), and 3.714 (2.828-4.877) (p for trend < 0.0001). Also, significant interaction was found between SUA and prehypertension in relation to the NAFLD risk (p for interaction < 0.05). CONCLUSIONS: SUA was significantly associated with NAFLD risk, independent of other metabolic risk factors, and SUA also had significant interaction with prehypertension regarding the risk of NAFLD.


Liang J.,Xuzhou Medical College | Liang J.,Xuzhou Institute of Medical science | Pei Y.,Nanjing Southeast University | Liu X.,Xuzhou Medical College | And 7 more authors.
Clinical Endocrinology | Year: 2015

Objective Insulin secretion and insulin resistance, which affect metabolic homoeostasis, each have a significant genetic component. Cyclin- dependent kinase 5 (CDK5) regulatory subunit-associated protein 1-like 1 (CDKAL1) rs10946398, a novel body mass index (BMI)-associated locus specifically in the Asian population, may impair insulin secretion and may be associated with insulin resistance and type 2 diabetes. Our objective was to investigate the impact of the rs10946398 polymorphism of CDKAL1 on insulin secretion, insulin resistance and glucose-related traits in the Chinese population. Subjects and Methods The study samples were based on a community-based health examination survey conducted in central China. Indices of insulin resistance and insulin secretion were derived from fasting glucose measurements and oral glucose tolerance tests (OGTTs). Using multivariate linear regression models, the relationships between the rs10946398 polymorphism of CDKAL1 and insulin secretion, insulin resistance and quantitative glucose-related traits were investigated in 2313 participants. Results The CDKAL1 rs10946398 C allele showed a significant association with decreased insulin secretion (β = -0·05, P < 0·0005), but not with insulin resistance (β = 0·02, P = 0·08). We also found that the CDKAL1 rs10946398 C allele was significantly associated with glucose-related traits (fasting glucose, fasting insulin, 2-h glucose and HbA1c). There was no significant relationship between rs10946398 and other metabolic traits. Conclusions rs10946398 of CDKAL1 was associated with markers of impaired insulin secretion. It is reasonable to infer that the relationship between CDKAL1 and metabolic diseases is mediated by its effect on glucose-related traits. © 2015 John Wiley & Sons Ltd.


Liu X.,Xuzhou Medical College | Liang J.,Xuzhou Medical College | Liang J.,Xuzhou Institute of Medical science | Qiu Q.,Xuzhou Medical College | And 6 more authors.
Cell Biochemistry and Biophysics | Year: 2014

Elevated blood pressure is regarded as an independent risk factor for cardiovascular diseases and diabetes. We examined the relation between hematocrit and pre-hypertension as well as the effect of sex, obesity, fasting glucose, and lipids in Chinese adults. The study samples were from a community-based health examination survey in China and included a total of 2,3691 patients with blood pressure in normal range. The odds ratios [ORs, 95 % confidence interval (CI)] of pre-hypertension across increasing quartiles of hematocrit were 1.000, 1.176 (1.050–1.318), 1.213 (1.081–1.363), and 1.364 (1.209–1.540) (P for trend < 0.001), when adjusted for age, sex, body mass index, glutamic-pyruvic transaminase, glutamic-oxalocetie transaminase, serum uric acid, glucose, and lipids. Associations were significant in both men and women, but not in individuals older than 60 years. In addition, low-density lipoprotein cholesterol significantly interacted with hematocrit (P for interaction <0.024). The associations were more evident in patients with low (P < 0.001) and median LDL-C levels (P < 0.013) than those with high glucose levels. Hematocrit was associated with pre-hypertension, and was independent of metabolic risk factors. These associations were not significant in older individuals and low-density lipoprotein cholesterol may modify these associations. © 2014, Springer Science+Business Media New York.


Zhu Y.,Xuzhou Medical College | Gong Y.,Xuzhou Central Hospital | Gong Y.,Xuzhou Medical College | Gong Y.,Xuzhou Institute of Medical science | And 12 more authors.
Journal of Clinical Hypertension | Year: 2014

The authors aimed to investigate the relationship between serum gamma-glutamyltransferase (GGT) and prehypertension, as well as the modification of other metabolic risk factors in a large cohort of Chinese individuals. The data were collected via a community-based health examination survey in central China. Blood pressure, body mass index (BMI), and levels of GGT, fasting blood glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lipid indicators were measured. In total, data from 18,302 patients with available biomarkers were included in the present study. Elevated blood pressure was associated with increased GGT concentration (P<.001). After adjusting for age, sex, BMI, fasting blood glucose, lipid indicators, AST, and family history of hypertension, the association between GGT levels and prehypertension remained significant (P=.021). The adjusted odds ratios (95% confidence interval) for prehypertension across quintiles of GGT level were 1.00, 1.057 (1.012-1.334), 1.068 (0.916-1.254), 1.024 (0.851-1.368), and 1.272 (1.027-1.593), respectively. In stratified analyses, the association between GGT levels and prehypertension was significant in women but was not significant in men. Moreover, additive effect of BMI and age on the effect of GGT levels on prehypertension (both P for interaction <.001) was observed. In summary, GGT levels were positively associated with prehypertension in women, independent of other metabolic factors. Furthermore, BMI and age may amplify the effects of GGT levels on prehypertension. These findings suggest that monitoring the levels of GGT could help in the diagnosis and monitoring of prehypertension. © 2014 Wiley Periodicals, Inc.


Zou C.,Central South University | Zou C.,Xuzhou Institute of Medical science | Qiu Q.,Xuzhou Medical College | Chen H.,Central South University | And 6 more authors.
Human and Experimental Toxicology | Year: 2016

The present study investigated the hepatoprotective role of selenium during alloxan-induced diabetes in rats. Male Wistar rats were divided into four groups, namely, normal control, selenium treated, diabetic, and selenium-treated diabetic. Diabetes was induced in the animals by injecting alloxan intraperitoneally at a dose rate of 150 mg/kg body weight. Selenium in the form of sodium selenite was supplemented to rats at a dose level of 1 ppm in drinking water, ad libitum for two time durations of 2 and 4 weeks. The effects of different treatments were studied on various parameters in rat liver, which included serum glucose levels, serum insulin levels, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lipid peroxidation (LPO), glutathione reduced (GSH), oxidized glutathione (GSSG), total glutathione (TG), superoxide dismutase (SOD), catalase (CAT), glutathione reductase, glutathione peroxidase, metallothionein (MT), and histoarchitecture. A significant increase in the serum glucose levels, LPO levels, and in enzyme activities of ALP, ALT, and AST was observed in diabetic rats which, however, got decreased significantly upon supplementation with selenium. On the contrary, decreased enzyme activities of GSSG, SOD, and CAT and depressed levels of GSH as well as serum insulin levels were observed in diabetic rats which got improved following selenium supplementation. Interestingly, MT levels were increased both in diabetic and selenium-treated diabetic rats. Further, marked alterations in histoarchitecture were seen in diabetic rats with the prominent features being congestion in sinusoids, lipid accumulation, and centrilobular hepatocyte degeneration. However, selenium treatment to diabetic rats showed overall improvement in the hepatic histoarchitecture. © The Author(s) 2015.


Qiu Q.,Xuzhou Medical College | Gong Y.,Xuzhou Medical College | Gong Y.,Xuzhou Institute of Medical science | Liu X.,Xuzhou Medical College | And 9 more authors.
Cell Biochemistry and Biophysics | Year: 2015

Serum uric acid (SUA) elevation has been previously related to impaired fasting glucose and type 2 diabetes. The present study was comprehensive to examine the associations between SUA and impaired glucose tolerance (IGT) in Chinese adults. For this purpose, data were collected from a community-based health examination survey conducted in Central China; 2-h glucose (OGTT) and SUA were measured in 1956 men and women. In multivariate models, SUA levels were significantly associated with an increasing trend of 2-h glucose (OGTT) (P for trend < 0.0001). The odds ratios (OR; 95 % CI) of IGT across increasing quartiles of SUA were 1.0, 1.354 (0.948–2.087), 1.337 (0.959–2.251), and 2.192 (1.407–3.416), after adjusting for age, sex, body mass index, waist circumference, fasting insulin, blood pressure, serum lipids, serum creatinine, and estimated glomerular filtration rate. (P for trend = 0.001). In addition, we found an additive pattern between SUA and triglyceride (TG; P = 0.038) or between SUA and low-density lipoprotein cholesterol (LDL-C; P = 0.041) in relation to IGT. SUA was related to IGT in the Chinese adults, independent of other conventional metabolic risk factors. TG and LDL-C might modify the associations. © 2015, Springer Science+Business Media New York.


Zou C.-Y.,Xuzhou Medical College | Zou C.-Y.,Xuzhou Institute of Medical science | Gong Y.,Xuzhou Medical College | Gong Y.,Xuzhou Institute of Medical science | And 2 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2014

Pancreatic β-cell is responsible for insulin secretion in response to the availability of nutrients. Type 2 diabetes mellitus (T2D) is the result of pancreatic β-cell failure to supply sufficient amount of insulin accompanied with decreased sensitivity of the body tissues to respond to insulin. The insulin secret ion apparatus of β-cel l is uniquely equipped with multiple metabolic and signaling steps that are under rigorous control. The metabolic machinery of β-cell is designed to sense the fluctuations in blood glucose level and supply insulin accordingly to the needs of body. Besides glucose, amino acids including glutamine and leucine and also fatty acids are known to either stimulate the β-cells directly or potentiate the glucose stimulated insulin secretion (GSIS) response. Glucose metabolism dependent GSIS is linked with the production of ATP that is needed for K+ ATP channel inhibition and influx of calcium, necessary for insulin granule exocytosis. Besides glucose metabolism, amino acid metabolism and lipid metabolism derived metabolites mediate the optimal glucose response of β-cells to secrete insulin. Metabolites derived from nutrient secretagogues that directly or indirectly participate in the enhancement of GSIS are considered as metabolic coupling factors. In this review, we will discuss the regulation of insulin secretion by β-cell keeping the recent developments in metabolic signaling in focus. The relevant metabolic pathways in pancreatic β-cell and their role in the control of fuelstimulated insulin secretion will be reviewed to arrive at a consensus picture with respect to the metabolic signaling of insulin secretion.


Liang J.,Xuzhou Medical College | Liang J.,Xuzhou Institute of Medical science | Qiu Q.,Xuzhou Medical College | Gong Y.,Xuzhou Medical College | And 10 more authors.
Journal of Clinical Hypertension | Year: 2015

The authors examined whether the adiponectin gene (ADIPOQ) variant was associated with blood pressure and arterial stiffness in Chinese adults. A genome-wide association study of the adiponectin variant rs864265 in the ADIPOQ gene was genotyped in a total of 2364 participants. After adjustment for sex, age, body mass index (BMI), fasting glucose, and lipids, participants carrying the T allele of rs864265 showed a greater increase in carotid-femoral pulse wave velocity (cfPWV) and systolic blood pressure (SBP). Further adjustment for blood pressure did not appreciably change the association with cfPWV. The authors found significant interactions between rs864265 and BMI, waist circumference, body fat percentage, and SBP in relation to cfPWV (P for interaction =035,.001,.003,.013, respectively). The T allele of rs864265 was associated with high blood pressure and arterial stiffness. BMI, body fat percentage, waist circumference, and SBP might modify the effects of genetic polymorphism on arterial stiffness. © 2015 Wiley Periodicals, Inc.


Chen H.,Xuzhou Medical College | Chen H.,Xuzhou Institute of Medical science | Qiu Q.,Xuzhou Medical College | Zou C.,Xuzhou Medical College | And 5 more authors.
Chemico-Biological Interactions | Year: 2015

Abstract In the present study, we have tried to unravel the role of Selenium supplementation in containing hyperglycemia by regulating enzymes activities involved in carbohydrate metabolism in liver of diabetic animals. Male wistar rats were divided into four groups: normal control, diabetic, Selenium treated control and Selenium treated diabetic group. Diabetes was induced in the animals by injecting alloxan intraperitoneally at a dose level of 150 mg/kg body weight. Selenium in the form of sodium selenite was supplemented to rats at a dose level of 1 PPM in drinking water, ad libitum for two time durations of 2 and 4 weeks. Animals were sacrificed and livers were excised for the analyses of enzymes involved in carbohydrate metabolism as well as the levels of glycogen. In-vitro 14C-d glucose uptake and its turnover were also assessed in liver slices of all the treatment groups using radiorespirometry. Selenium supplementation to the diabetic rats normalized the enzyme activities of glucose-6-phosphatase, lactate dehydrogenase and glycogen phosphorylase as well as restored the glycogen levels to within the normal limits which were altered during diabetes. Interestingly, when Selenium was supplemented to diabetic rats, 14C-d glucose uptake and its turnover showed a statistically significant increase in their values which however, were decreased in diabetic rats. In conclusion, Selenium mediates insulin-like role during diabetes by tending to normalize the altered activities of glucose metabolizing enzymes and also improves the glucose uptake and its metabolism by the liver. © 2015 Elsevier Ireland Ltd. All rights reserved.


Ren S.-p.,Xuzhou Institute of Medical science | Han G.-y.,Xuzhou Institute of Medical science | Shi L.,Xuzhou Institute of Medical science | Tan K.,Xuzhou Institute of Medical science | And 3 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Cytokine induced killer (CIK) cells are cultured mostly in 10% serum, but there have been few studies describing the effects of serum concentration on growth characteristics of CIK cells. OBJECTIVE: To investigate the proliferation of CIK cells in different concentrations of serum. METHODS: CIK cells were cultured with 10%, 20% and 30% fetal bovine serum supplemented with recombinant human interleukin (IL-2). The effects of serum concentration on cell cycle were analyzed using flow cytometry. The proliferation of CIK in different concentrations of serum was determined by MTT assay and the growth characteristics of CIK cells were observed. RESULTS AND CONCLUSION: The proliferation rate and proliferation index of CIK cells were the lowest in 10% serum and highest in 30% serum. There was statistical significance in proliferation rate among three groups (P < 0.05 or P < 0.01). The proliferation of CIK cells cultured are serum concentration-dependent in vitro.

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