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Zhai X.-X.,Traditional Chinese Medicine Hospital of Xuzhou City | Ding J.-C.,Xuzhou Central Hospital | Tang Z.-M.,Traditional Chinese Medicine Hospital of Xuzhou City
Cell Biochemistry and Biophysics | Year: 2015

The objective of this study was to determine the effect of resveratrol (Res) treatment on pathological scar fibroblasts and the changes in TGF-β1/Smads signaling pathway. For this purpose, cultured pathological scar fibroblasts were treated with various concentrations of Res (10, 50, and, 100 µmol/l), and the morphological changes in target cells were studied using scanning electron microscopy (SEM). The cellular proliferation was assessed by MTT assay; the mRNA and protein expressions of TGF-β1 and Smad-2,3,4,7 were determined by reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence assay, respectively. We found that Res-treated fibroblasts exhibited the typical apoptotic morphological changes. As shown by MTT assay, the OD values of Res-treated fibroblasts, as a measure of cell growth, were significantly lower than those of controls (P < 0.05). In addition, as compared to controls, TGF-β1 and Smad-2,3,4 mRNA/protein expression decreased but those of Smad7 increased in a dose-dependent manner (P < 0.05). It was, therefore, concluded that Res treatment inhibited the pathological scar fibroblast proliferation and induced cell apoptosis through the mechanism involving downregulation of TGF-β1, Smad-2,3,4, and upregulation of Smad7. © 2015, Springer Science+Business Media New York. Source


Zhai X.-X.,Traditional Chinese Medicine Hospital of Xuzhou City | Ding J.-C.,Xuzhou Central Hospital | Tang Z.-M.,Traditional Chinese Medicine Hospital of Xuzhou City | Li J.-G.,Traditional Chinese Medicine Hospital of Xuzhou City | And 4 more authors.
Oncology Letters | Year: 2016

The aim of the present study was to investigate the effect of resveratrol on cell apoptosis, ability of telomerase and the human telomerase reverse transcriptase (hTERT) protein expression in human A431 epidermoid carcinoma cells. A431 cells were treated with different concentrations of resveratrol, and the cell appearance was then observed under a microscope. In addition, the cell proliferation was examined using an MTT assay, and the ability of telomerase was detected using telomeric repeat amplification protocol-polymerase chain reaction-ELISA. Resveratrol significantly inhibited the ability of telomerase and decreased the expression of hTERT protein in a concentration-dependent manner. In conclusion, resveratrol is capable of downregulating the expression of hTERT protein and inhibits the ability of telomerase of A431, which is an important mechanism of action of resveratrol with regard to inhibition of A431 cell proliferation. © 2016, Spandidos Publications. All rights reserved. Source

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